Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,143,110 G→A 100% V163V (GTG→GTA USA300HOU_2026 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,143,1100GA100.0% 49.6 / NA 17V163V (GTG→GTAUSA300HOU_2026hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/4);  total (13/4)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTGGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACGG  >  CP000730/2142975‑2143236
                                                                                                                                       |                                                                                                                               
gCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCAtt                                                                                                                            >  1:160243/1‑141 (MQ=255)
   aaTTATTATGTCTCGAAACATACCGTGATTGAACTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATttat                                                                                                                         <  1:134631/141‑1 (MQ=255)
         tattGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAAGCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGc                                                                                                                  <  1:59818/141‑1 (MQ=255)
              cTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGCCGGACAGTTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTacaa                                                                                                             <  1:66069/141‑1 (MQ=255)
                                     ttcttTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCa                                                                                      >  2:198343/1‑141 (MQ=255)
                                           gTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAAAAGTAAGTTCATTAATATTTGCATTAGcc                                                                                >  2:936/1‑140 (MQ=255)
                                                         tCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTGATTAAGAAATATGTAGTTAATTAATATTTGCATTAGCACATAATGATTTCaa                                                                  >  1:280103/1‑141 (MQ=255)
                                                              tattaGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATGAAAGGTAATCGTATCGTAGCATTTATTATTGGTACAACAGTAAGTTCATTAATATTTGCATTATCACATAATGATTTCAAATTTa                                                             >  2:157189/1‑141 (MQ=255)
                                                                     agaaTATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGt                                                      >  2:17341/1‑141 (MQ=255)
                                                                           tGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTAttt                                                >  1:63694/1‑141 (MQ=255)
                                                                                 cAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTAt                                          >  1:246352/1‑141 (MQ=255)
                                                                                    aaaaGGAATGTTTGGANAATCATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATggg                                       <  1:223527/141‑1 (MQ=255)
                                                                                                          tttAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCTTTTTTTTTTTAgtttat                 >  2:8565/1‑136 (MQ=255)
                                                                                                          tttAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCatat                 >  1:218008/1‑141 (MQ=255)
                                                                                                          tttAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCatat                 >  2:248512/1‑141 (MQ=255)
                                                                                                                      aaaGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCACCTTTACTCCAGGTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACaaaa     >  1:208067/1‑141 (MQ=255)
                                                                                                                         ggTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAAAgg  >  2:22518/1‑141 (MQ=255)
                                                                                                                                       |                                                                                                                               
GCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTGGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACGG  >  CP000730/2142975‑2143236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: