Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,315,088 T→A 100% K61I (AAA→ATA)  USA300HOU_2172 ← MFS family major facilitator transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,315,0880TA100.0% 58.4 / NA 20K61I (AAA→ATA) USA300HOU_2172MFS family major facilitator transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (7/13);  total (7/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATTTATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGTCCAATCCAATT  >  CP000730/2314966‑2315224
                                                                                                                          |                                                                                                                                        
tCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTg                                                                                                                        <  2:234459/141‑1 (MQ=255)
   cATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGccc                                                                                                                     >  2:12498/1‑141 (MQ=255)
   cATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGCCAATTGCCCCTGccc                                                                                                                     >  1:216064/1‑141 (MQ=255)
     tatatcATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTAGGAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCcac                                                                                                                   <  2:105767/141‑1 (MQ=255)
     tatataATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCcac                                                                                                                   <  2:198959/141‑1 (MQ=255)
          aataatGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACt                                                                                                              >  1:260093/1‑141 (MQ=255)
                    cTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGACCCTGCCCACAGACTTGGTaatgat                                                                                                    >  1:182789/1‑141 (MQ=255)
                          ggAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGt                                                                                              <  2:278123/141‑1 (MQ=255)
                                   gttaaaCTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTg                                                                                     <  2:277119/138‑1 (MQ=255)
                                    tGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGc                                                                                    <  1:118906/141‑1 (MQ=255)
                                    tGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGc                                                                                    <  2:225345/141‑1 (MQ=255)
                                                  aGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATc                                                                      >  1:118178/1‑141 (MQ=255)
                                                          aaaaaTTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATa                                                              <  1:95065/140‑1 (MQ=255)
                                                           aaaaTTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATaa                                                             <  2:237420/141‑1 (MQ=255)
                                                           aaaaGTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCTACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATaa                                                             <  1:238161/141‑1 (MQ=255)
                                                                 tcAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTTCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCg                                                       <  2:7374/140‑1 (MQ=255)
                                                                  cAGTTACGAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGt                                                      <  2:150096/141‑1 (MQ=255)
                                                                          aaaaaTGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACCGCGTTATCATaa                                              >  2:251823/1‑141 (MQ=255)
                                                                                                     aGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACCCTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCACATAAACTGCGTTATGATAAGTGGTAAGTACAAACCACTTTAGCCCt                   >  2:171687/1‑141 (MQ=255)
                                                                                                            ccccTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGtc            <  2:215316/140‑1 (MQ=255)
                                                                                                                      atatatatCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGTCCAATCCCAtt  <  2:216064/141‑1 (MQ=255)
                                                                                                                      atatatatCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGTCCAATCCAAtt  <  2:260093/141‑1 (MQ=255)
                                                                                                                          |                                                                                                                                        
TCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATTTATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGTCCAATCCAATT  >  CP000730/2314966‑2315224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: