Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | CP000730 | 2,315,088 | T→A | 100% | K61I (AAA→ATA) | USA300HOU_2172 ← | MFS family major facilitator transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | CP000730 | 2,315,088 | 0 | T | A | 100.0% | 58.4 / NA | 20 | K61I (AAA→ATA) | USA300HOU_2172 | MFS family major facilitator transporter |
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (7/13); total (7/13) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATTTATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGTCCAATCCAATT > CP000730/2314966‑2315224 | tCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTg < 2:234459/141‑1 (MQ=255) cATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGccc > 2:12498/1‑141 (MQ=255) cATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGCCAATTGCCCCTGccc > 1:216064/1‑141 (MQ=255) tatatcATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTAGGAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCcac < 2:105767/141‑1 (MQ=255) tatataATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCcac < 2:198959/141‑1 (MQ=255) aataatGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACt > 1:260093/1‑141 (MQ=255) cTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGACCCTGCCCACAGACTTGGTaatgat > 1:182789/1‑141 (MQ=255) ggAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGt < 2:278123/141‑1 (MQ=255) gttaaaCTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTg < 2:277119/138‑1 (MQ=255) tGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGc < 1:118906/141‑1 (MQ=255) tGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGc < 2:225345/141‑1 (MQ=255) aGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATc > 1:118178/1‑141 (MQ=255) aaaaaTTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATa < 1:95065/140‑1 (MQ=255) aaaaTTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATaa < 2:237420/141‑1 (MQ=255) aaaaGTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCTACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATaa < 1:238161/141‑1 (MQ=255) tcAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTTCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCg < 2:7374/140‑1 (MQ=255) cAGTTACGAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGt < 2:150096/141‑1 (MQ=255) aaaaaTGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACCGCGTTATCATaa > 2:251823/1‑141 (MQ=255) aGCAATCGCCCTTTATCATATATATCGGCAATTGCCCCTGCCCACACCCTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCACATAAACTGCGTTATGATAAGTGGTAAGTACAAACCACTTTAGCCCt > 2:171687/1‑141 (MQ=255) ccccTTTATCATATATATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGtc < 2:215316/140‑1 (MQ=255) atatatatCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGTCCAATCCCAtt < 2:216064/141‑1 (MQ=255) atatatatCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGTCCAATCCAAtt < 2:260093/141‑1 (MQ=255) | TCGCATATATAATAATGACGCTAATTGGAATTGCAGTGAAACTATACGTGAGCACACATAAAATTGCAGTTACTAAAAATGATGATGAAATTGTAATTCTCAGCAATCGCCCTTTATCATATTTATCGGCAATTGCCCCTGCCCACACACTTAGTAATAATATTGGTACAAGTCTGCAAAAATTGACAATCCCCAGATAAACTGCGTTATGATAAGTGGTAAGTACAAACCAATTTAGCCCTATTTGTCCAATCCAATT > CP000730/2314966‑2315224 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |