| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,342,380 | A→C | 100% | L78V (TTG→GTG) | USA300HOU_2199 ← | possible HAD superfamily hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,342,380 | 0 | A | C | 99.8% | 82.5 / ‑7.3 | 27 | L78V (TTG→GTG) | USA300HOU_2199 | possible HAD superfamily hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); major base C (17/8); minor base T (1/0); total (19/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCAAATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTT > CP000730/2342247‑2342513 | ttATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTAAACATTTTAGCACTCACATcctaa > 1:235706/1‑137 (MQ=255) ttGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGAATTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAAGTCACATCAGAAAGGat > 1:44888/1‑141 (MQ=255) gcAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAAGGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACAACAGAAAAGATATGt < 2:146916/141‑1 (MQ=255) aaTATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATCTGTAATgg > 2:109368/1‑141 (MQ=255) tatGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTATTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGttt > 1:54662/1‑141 (MQ=255) aCGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTaaa > 1:107523/1‑141 (MQ=255) tatcttcaaATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACCTCAGAAAAGATATATAATGGTTTAACCGTCATATTAACTTGAt > 2:257982/1‑141 (MQ=255) ttcaaATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCAAATCAGAAAAGATATGTAGTGGTTTAAACGTCATATTAACTTGATCCAt > 1:267064/1‑141 (MQ=255) tcaaATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATCTTAACTTGATCCAtt > 2:225722/1‑141 (MQ=255) aaTGGTTCGCTCAAGACTTTCTGTTTATATGTTTCTGATTCAACATATTCGCCATTATTCAAACAGAACATTTTAGAAATCACTTCAAAAAAAATATTTACTGGTTTAAACGTCATATTAACTTGATCCATTTTATCCAta > 2:112362/1‑140 (MQ=255) tCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACtt > 1:247035/1‑141 (MQ=255) tCGCTCAAGACTTTCTCTTCATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACtt > 1:159714/1‑141 (MQ=255) gACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGt > 2:159613/1‑141 (MQ=255) tttatttTTATATGTTTCTGATTCAACATAATAGCCATTATTCCAAACGCAGATAGTAATACACATATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACt > 2:60490/1‑141 (MQ=255) ttttATATGTTTCTGATTCAACCTATTCGCCATTATTCCAAGTTAACATTTTAGAACTCACATCAGAAAATATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACTCTTTGAAATGTACTTCCTg < 1:141428/141‑1 (MQ=255) tgtataTGTTTCTGATTCAACATATTCTCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGt < 1:257982/139‑1 (MQ=255) ttATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTc < 1:285112/141‑1 (MQ=255) cTGATTCAACATATTCGCCATTATTCCAGGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCACTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATAcagcag < 1:161746/141‑1 (MQ=255) tGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGt < 2:155657/141‑1 (MQ=255) acAACAGAGTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGAta > 2:2853/2‑140 (MQ=255) aCATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACt > 1:46222/1‑141 (MQ=255) ttCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCatt > 1:200632/1‑141 (MQ=255) gCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCattatt > 1:253276/1‑141 (MQ=255) cAACTGAACATCTTAGAACTCACATCAGAAAAGATATGTAATGGATTAAACGTCATATTAACTTGATCCATTTTACCCATTACCCCTTAAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATtagttagt < 2:230152/141‑1 (MQ=255) gAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTc > 2:252260/1‑141 (MQ=255) tAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAAtt > 1:1984/1‑141 (MQ=255) gAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAAtttt < 2:84463/141‑1 (MQ=255) | TTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCAAATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTT > CP000730/2342247‑2342513 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |