Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,504,894 C→T 18.0% intergenic (+198/+206) USA300HOU_2365 → / ← gltT hypothetical protein/DAACS family dicarboxylate/amino acid:sodium (Na+) or proton (H+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,504,8940CT18.0% 130.2 / 13.4 65intergenic (+198/+206)USA300HOU_2365/gltThypothetical protein/DAACS family dicarboxylate/amino acid:sodium (Na+) or proton (H+) symporter
Reads supporting (aligned to +/- strand):  ref base C (29/24);  new base T (3/9);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTT  >  CP000730/2504758‑2505033
                                                                                                                                        |                                                                                                                                           
aGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCACCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGt                                                                                                                                         >  1:189178/1‑141 (MQ=255)
aGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCACCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGt                                                                                                                                         >  1:46019/1‑141 (MQ=255)
        tCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTgc                                                                                                                                 <  1:118323/141‑1 (MQ=255)
          cAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTgcgc                                                                                                                               <  1:261726/141‑1 (MQ=255)
             tttGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCtaa                                                                                                                            <  2:46964/141‑3 (MQ=255)
                 gAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATACAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAgcg                                                                                                                        >  2:222289/1‑141 (MQ=255)
                   aTCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCgca                                                                                                                      >  1:96462/1‑141 (MQ=255)
                    tCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCgcat                                                                                                                     >  1:107481/1‑141 (MQ=255)
                       ttttACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACCACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCgcatgca                                                                                                                  <  2:138196/141‑1 (MQ=255)
                        tttACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAGCAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCgcatgcat                                                                                                                 <  2:256014/141‑1 (MQ=255)
                        tttACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCgcatgcat                                                                                                                 <  1:32045/141‑1 (MQ=255)
                           aCTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATaaa                                                                                                              <  2:180817/141‑1 (MQ=255)
                                tgctTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGccc                                                                                                         >  1:226384/1‑141 (MQ=255)
                                tgctTTTTATATTTGAAATACTTCCATATTGAATAGCTCCACTTGCTGTTCGCCTGCGCTTGTCGCATGCATAAAAAGCCGCAACAACCGAAGTCCACGTCCCTCCGGTTCGCCTGCGCTTTAGCGCATCCATAAAAGccc                                                                                                         >  2:275045/1‑141 (MQ=21)
                                    ttttATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaa                                                                                                     <  1:207570/141‑1 (MQ=255)
                                       tataTTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaacaa                                                                                                  >  1:134595/1‑141 (MQ=255)
                                           tttGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTGGCGCATGCATAAAAGCCCCTAACAACCTg                                                                                              >  2:38081/1‑141 (MQ=255)
                                           tttGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTg                                                                                              <  1:43471/141‑1 (MQ=255)
                                           ttggAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATTAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTg                                                                                              <  2:49663/138‑1 (MQ=255)
                                            ttGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGa                                                                                             >  1:255126/1‑141 (MQ=255)
                                                      ttCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACg                                                                                   >  1:177398/1‑141 (MQ=255)
                                                        cATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTCCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTTCgat                                                                                 >  2:158766/1‑139 (MQ=255)
                                                            ttGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTACACATGCATAGAAGCCCCTAACGACATGAGGTCACTGCGCTTCTGTTCGCCTGCGCTTTCGGTATTGCTTAAAACACACTAACAACGTGAGGTCACTATGCTTCgg                                                                             >  2:123365/1‑141 (MQ=255)
                                                              gAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTCCGCCTGCGCGTTAGCGCATGCATAAAAGCCCCTAACACCCTGAGGTCACTACGCTTCGGtt                                                                           <  1:122448/141‑1 (MQ=255)
                                                              gAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCCCCGGTTCGCCTGCGCTTTAACGCATGCATAAAAGCCCCTAACAACCGGGGGTCGCTGCGCTTCggat                                                                           >  2:98448/1‑139 (MQ=18)
                                                                aTAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCCGTTCGCCGGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCg                                                                         <  2:116252/141‑1 (MQ=255)
                                                                aTAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCCAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCg                                                                         <  2:285122/141‑1 (MQ=255)
                                                                 tAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGGGCTTTAGCGCCTGCATAAAAGCCCCTAACAACCTGAGGTCACTCCGCTTCGTTTCGc                                                                        >  1:135653/1‑141 (MQ=255)
                                                                     cccACTTGCCGTCCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTgc                                                                    <  2:15605/140‑1 (MQ=255)
                                                                        aCTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCAGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGACTGTGCt                                                                 >  2:213235/1‑141 (MQ=255)
                                                                         cTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCta                                                                <  2:35576/141‑2 (MQ=255)
                                                                             ccGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCCTGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCCGGGCTTTatc                                                            >  2:210402/1‑139 (MQ=255)
                                                                             ccGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAgc                                                            >  1:238157/1‑141 (MQ=255)
                                                                                ttCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTTAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCgca                                                         <  2:153646/141‑1 (MQ=255)
                                                                                   gCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCgcatgc                                                      >  1:119988/1‑141 (MQ=255)
                                                                                    ccTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATCAAAGCCCCTAACAACCTGAGGTCACTACGATTCCGTTCGCCTGCGCGTTAgcaaaagca                                                     >  2:223591/1‑134 (MQ=14)
                                                                                     cTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCgcatgcat                                                    <  1:89008/141‑1 (MQ=255)
                                                                                              gcggATGCATAAAGGCGCCTAACAACCTGAGGTCACTCCGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTccc                                           <  2:123257/141‑1 (MQ=11)
                                                                                              gcgcATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTAGGGGTCGGTTCGCCTAAGATTTAGCGGATGCGTAAAAGTgcc                                           >  2:80425/1‑141 (MQ=14)
                                                                                                 catgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCgcatgca                                                                                                                  >  2:190888/1‑67 (MQ=255)
                                                                                                 catgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCgcatgca                                                                                                                  >  1:254705/1‑67 (MQ=40)
                                                                                                   tgcatAAAAGTCCCTAACCACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTaaca                                      <  1:275045/141‑1 (MQ=11)
                                                                                                   tgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTaaca                                      <  1:237681/141‑1 (MQ=255)
                                                                                                      atAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAAcc                                   <  2:134595/141‑1 (MQ=255)
                                                                                                       tAAACGCCCCTAACCACCTGGGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCt                                  <  2:209029/141‑1 (MQ=255)
                                                                                                             tcccTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCCAACAACCTGAGGTCACAAGGCGTCCAATCGCCGACGCTGTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGtt                            >  2:102410/2‑140 (MQ=11)
                                                                                                             ccccTAACAACCGGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTc                            <  1:220136/141‑1 (MQ=255)
                                                                                                                  aacaacCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTAc                       >  1:276983/1‑141 (MQ=255)
                                                                                                                    caacCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCGGAGGTTACTAGGc                     >  2:150695/1‑141 (MQ=255)
                                                                                                                      acCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCtt                   >  1:196260/1‑141 (MQ=255)
                                                                                                                        cTGAGGTCACTACGCCTCCGTTCCCCTGCGCTTTAGCGCATGCATAAAAGCCCCCAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTcc                 <  2:107481/141‑1 (MQ=14)
                                                                                                                         tGAGGTCACTACGCCTCCGTGCGCCTGCGTTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTCTAGCGAATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCg                <  1:223591/141‑1 (MQ=14)
                                                                                                                            ggTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAAGAACCTGAGGTCACTACGCTTCCGTTc             >  1:175146/1‑141 (MQ=255)
                                                                                                                            ggTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCCGAGGTTACTGCGGTTCCGTTc             >  2:241142/1‑141 (MQ=14)
                                                                                                                             gTCACTCCGCTTCGGTTCGGCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCg            <  1:123365/141‑1 (MQ=14)
                                                                                                                             gTCACTACGCTCCCGGTCGCCGGCGCTTTTGCGCATGCATAAAAGCCCCTAACAACCTAAGATCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCg            <  1:158766/141‑1 (MQ=14)
                                                                                                                              tCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGc           >  2:147151/1‑141 (MQ=255)
                                                                                                                               cACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTGGcc          >  1:241569/1‑141 (MQ=255)
                                                                                                                                aCTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCt         >  1:227438/1‑141 (MQ=255)
                                                                                                                                  cccgcTTCGGGTCGGCCGCGCTTTAGCGCATGCATAAAAGCCCCTACCAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTgc       <  1:11775/139‑1 (MQ=11)
                                                                                                                                    cgcTTCCGTTCCCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTCAGCTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTgcgc     <  1:222289/141‑1 (MQ=14)
                                                                                                                                    cgcTCCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTgcgc     <  2:189178/141‑1 (MQ=255)
                                                                                                                                     gcTTCGGTCCGCCTGCGTTTCTGCGCGTGCATAAAGGCTCCTAACAACCTGGGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCt    <  2:232160/141‑1 (MQ=14)
                                                                                                                                      cTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCtt   <  1:130605/141‑1 (MQ=255)
                                                                                                                                       tCCGGTTCGCCCGCGCTGTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCttt  <  1:106333/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                           
AGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTT  >  CP000730/2504758‑2505033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑