| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,679,650 | G→A | 100% | C45Y (TGT→TAT) | USA300HOU_2536 → | possible secretory antigen |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,679,650 | 0 | G | A | 93.4% | 69.0 / ‑3.1 | 27 | C45Y (TGT→TAT) | USA300HOU_2536 | possible secretory antigen |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (16/9); total (16/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTCACTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTC > CP000730/2679515‑2679786 | aaaTGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTCACTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTTATTATTATAGTAAAAACCAATAGACAt > 1:102689/1‑141 (MQ=255)aaaTGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTCACTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACAt > 1:102685/1‑141 (MQ=255) aTGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTCACTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACAtgg > 1:220446/1‑141 (MQ=255) aaaGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTCACTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCAtttga > 1:43821/1‑139 (MQ=255) attaATTGCTTTTGCAGTACTTTTCTCAGCAGATTTCACTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCgg < 1:250969/141‑1 (MQ=255) ttaATTGCTTTTGCAGTACTTTTCTCAGCAGATTTCACTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGt < 2:25568/141‑1 (MQ=255) tgtttttGCATTACTTTTCTCAGCAGATTTCACTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAAcc < 2:101664/138‑1 (MQ=255) tttCCCCGCAGATTTCACTTATCACTTCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATg < 1:64676/141‑1 (MQ=255) ttCTCAGCAGATTTCACTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGAtag > 2:65414/1‑139 (MQ=255) tctcAGCAGATTTCACTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATggg < 1:156716/141‑1 (MQ=255) tctcAGCAGATTTCACTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAAACTGTTTATAATAGATggg > 2:261289/1‑141 (MQ=255) ctcAGCAGATTTCACTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATgggg > 1:58183/1‑141 (MQ=255) cagcagATTTCACTTATCAATCCGTAGAACAAAAGCAACAATCGCATGCCGCAGTTAATTATTATAATAAAAACCAAAATACATGGTAGGCAATTAAACTTCAAGAACAAATCAGTAAACCAAATTCTaaaacataaggca > 2:20526/1‑130 (MQ=255) cagATTTCACTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATg < 2:13674/141‑1 (MQ=255) cACTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTaaaaa < 1:265125/141‑1 (MQ=255) aTCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACACGTCGGTAAACCTGTTTCTAATAAATGGGGCAATGCTAAAAATTGGTAttt > 2:87879/1‑140 (MQ=255) cGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACaa > 1:223501/1‑141 (MQ=255) gaactaaccagtccaTCGCATGGCGCAATTAAGTACTATAGTAAAAACCAATGTACAGTGTGGGCATTTAAACGTCGCGCACAACTCGGTAAACCTGTTTCTAAAAGATGGGGCAATGCTACAAATTGGTATTACAATGCa < 2:244516/127‑1 (MQ=255) aCAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACg < 2:162651/141‑1 (MQ=255) tCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTAGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAAGGCTAAAAAGTGGTATTACAATGCACATGAATCaaa > 1:120939/1‑141 (MQ=255) aTGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTACACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGa > 2:7559/1‑141 (MQ=255) aTGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGGTGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAAACAAAATATgaga > 2:7539/1‑141 (MQ=255) tGCCGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGAc > 1:153037/1‑141 (MQ=255) tGCCGAAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGAc < 2:38875/141‑1 (MQ=255) cGCAGTTAATTATTATAGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTgg > 1:170889/1‑141 (MQ=255) ataGTAAAAACCAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGaa < 1:116274/141‑1 (MQ=255) cAATATACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAAAAAACTTGCTGTc > 2:248699/1‑141 (MQ=255) | AAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTCACTTATCAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTC > CP000730/2679515‑2679786 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |