Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,161 T→C 27.7% I131V (ATT→GTT)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,1610TC27.7% 361.9 / 201.3 316I131V (ATT→GTT) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base T (109/115);  new base C (38/49);  total (147/169)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 318 total aligned reads displayed.

TTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTT  >  CP000730/36022‑36299
                                                                                                                                           |                                                                                                                                          
tttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAAt                                                                                                                                            >  1:315979/1‑140 (MQ=255)
 ttcttACTTTAATATAACGGTGATCGTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAAtt                                                                                                                                           >  1:160258/1‑140 (MQ=255)
   cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAc                                                                                                                                         <  1:132817/140‑1 (MQ=255)
     tACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCt                                                                                                                                       >  1:101259/1‑140 (MQ=255)
     tACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCt                                                                                                                                       <  2:164059/140‑1 (MQ=37)
     tACTTTAATATGACGGTGATCCTGCTCAATGAGGTTATTCAGATATTTCGATGGACAATGGCAGTCAGGGTTAAGTTTAAACGCTTTCATTACTTTAACCATTGCCACCTTCGTTGAAGGTGCCTGATCTGTAATTACCt                                                                                                                                       <  1:124226/140‑1 (MQ=11)
       ggttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCttt                                                                                                                                     <  2:157348/138‑1 (MQ=25)
       cTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCttt                                                                                                                                     <  1:180521/140‑1 (MQ=255)
       cTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCttt                                                                                                                                     <  2:253630/140‑1 (MQ=255)
       cTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCttt                                                                                                                                     <  1:224095/140‑1 (MQ=255)
         ttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTg                                                                                                                                   >  1:5329/1‑140 (MQ=255)
           aaTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAg                                                                                                                                 >  2:53384/1‑140 (MQ=25)
            atatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTGCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAgg                                                                                                                                <  1:80793/140‑1 (MQ=11)
             tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTTAGGt                                                                                                                               <  2:221763/140‑1 (MQ=255)
             taggACGGTTATCTTGCTCAATGAGGTTAATCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGt                                                                                                                               <  1:228736/137‑1 (MQ=255)
              atGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTGAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGtt                                                                                                                              >  1:215102/1‑140 (MQ=37)
                  cGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAcc                                                                                                                          <  1:176530/140‑1 (MQ=11)
                  cGGGGATCTTGCTCAATGGGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCATTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                          <  2:160263/140‑1 (MQ=11)
                   ggTGAGCTTGGTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCa                                                                                                                         <  2:266889/140‑1 (MQ=11)
                     tgtttttGCTGAGTGGGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTTCTTTAACCATTGTTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                       <  2:312709/135‑1 (MQ=11)
                     tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTCCTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTCCCTTTTGAGGTTTACCaaa                                                                                                                       <  1:238705/140‑1 (MQ=255)
                     tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                       <  2:244050/140‑1 (MQ=17)
                      gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAt                                                                                                                      <  1:265400/140‑1 (MQ=12)
                       aTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGCGGTTTAACAAGtt                                                                                                                     >  2:254663/1‑140 (MQ=11)
                       aTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAtt                                                                                                                     >  2:8386/1‑140 (MQ=16)
                         cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                   >  1:210639/1‑140 (MQ=16)
                         cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                   >  2:18484/1‑140 (MQ=11)
                         cTTGCCCAATGGGTTTTGCCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                   <  2:277773/140‑1 (MQ=11)
                          ttGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGtt                                                                                                                  >  1:49255/1‑140 (MQ=11)
                           tGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGttt                                                                                                                 <  1:259901/140‑1 (MQ=11)
                              tCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAAt                                                                                                              >  1:146583/1‑140 (MQ=255)
                                aaTGGGGTTATTTAGATATTTCCATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTCTGTTGAAGCTGCCTGATCAGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATga                                                                                                            <  2:146300/140‑1 (MQ=11)
                                aaTGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATga                                                                                                            <  1:105085/140‑1 (MQ=33)
                                 atggtgTTATTCATATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgag                                                                                                           <  2:127203/135‑1 (MQ=11)
                                 aTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgag                                                                                                           <  1:217482/140‑1 (MQ=12)
                                      gTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTTTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                                                      >  1:155856/1‑140 (MQ=12)
                                       ttATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAGCTTCGTTGAAGGTGCCTGATCTGTAATTACCGTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                                                     <  2:93107/140‑1 (MQ=255)
                                       ctATTCAGATATTTCGATGTACAATGACAGGCAGGTTTAAGTGTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCACATATATAACCACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGttt                                                                                                     <  2:1506/139‑1 (MQ=11)
                                        tATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACTAAATTGTTTAATGAGACGTTTg                                                                                                    <  2:233853/140‑1 (MQ=11)
                                              gATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGTGACGTTTGATAAAc                                                                                              >  1:88053/1‑140 (MQ=12)
                                               atatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGACGGTGCCTGATATGTAATTACCTTTTGAGGCATACCAAATTGTTTAATGAGACGTTTGATAAACg                                                                                             >  2:112576/1‑140 (MQ=11)
                                               atatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACg                                                                                             <  1:67963/140‑1 (MQ=16)
                                                tatGTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGc                                                                                            <  2:32135/140‑1 (MQ=16)
                                                      gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                                                      >  2:207605/1‑140 (MQ=16)
                                                      gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                                                      >  1:294531/1‑140 (MQ=16)
                                                      gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAAACGTTTGATAAACGCATATGc                                                                                      >  1:35503/1‑140 (MQ=12)
                                                      gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGCTTAATGAGACGTTTGATAAACGCATATGc                                                                                      >  1:96325/1‑140 (MQ=255)
                                                        tGTACAATGACAGTCAGGTTGAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCGTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg                                                                                    >  1:195332/1‑140 (MQ=11)
                                                          tACAATGGCAGTCAGGTTTAAGTTTAAACGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGaa                                                                                  >  1:284743/1‑140 (MQ=255)
                                                           aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTCACTTCGTTGAAGGTGTCTGTTCTGTTATTTCCTTTTGATGTGTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAAt                                                                                 >  1:302752/1‑140 (MQ=11)
                                                           aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAAt                                                                                 <  1:205673/140‑1 (MQ=17)
                                                           aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAAt                                                                                 <  2:175864/140‑1 (MQ=17)
                                                           aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAAt                                                                                 >  1:307215/1‑140 (MQ=12)
                                                            cAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                                                >  2:174701/1‑140 (MQ=9)
                                                            cAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGCTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAAACGTTTGATAAACCCATATGCTGAAta                                                                                >  2:403/1‑139 (MQ=11)
                                                             aaTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                               >  2:69881/1‑140 (MQ=2)
                                                              aTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                                              <  1:198880/140‑1 (MQ=11)
                                                              aTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                                              <  1:312018/140‑1 (MQ=32)
                                                               tgccaatCAGGTTTAAGTTTAAAAGCTTTACTTACTTTTACCATTGCTACCTTCGGTGAAGGTGTCTGATCTGTAATTACCTTTTGAGGTTTACCATATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAtt                                                                             <  1:46121/134‑1 (MQ=1)
                                                               tGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAtt                                                                             >  1:275125/1‑140 (MQ=11)
                                                                gACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCATGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGCATGATTa                                                                            >  2:299041/1‑140 (MQ=11)
                                                                                                   ttAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                         <  2:108184/140‑1 (MQ=11)
                                                                                                      accATTGCTACCTTCGTTGGAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                      <  1:41257/139‑1 (MQ=18)
                                                                                                       ccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTccc                                     >  1:221824/1‑140 (MQ=32)
                                                                                                         aTTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGAGAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                   <  1:301686/140‑1 (MQ=255)
                                                                                                         aTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                   >  1:254323/1‑140 (MQ=32)
                                                                                                          ttGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTAGATAAACGCATATGCTGAGTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctct                                  >  2:273592/1‑140 (MQ=17)
                                                                                                             caacctttctggAAGGTGCCTGAAATGTAATTACCTTTGGAGGTTTAGCAACTTGTTTAATGCGACGTTTGATAAACGAATATATTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                               <  1:75118/129‑1 (MQ=11)
                                                                                                             cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATTCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgta                               >  1:247669/1‑138 (MQ=17)
                                                                                                             cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                               <  1:282557/140‑1 (MQ=33)
                                                                                                             cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                               <  2:141348/140‑1 (MQ=33)
                                                                                                              tcccttccttGAAGTTGCCTCATCTGTAACTACCGTTTGAGGCTTACCAAATTGTTCAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTAt                              <  1:156782/138‑1 (MQ=11)
                                                                                                              tACCTTCTGTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAt                              <  2:212362/140‑1 (MQ=18)
                                                                                                              tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgtct                              <  2:260179/140‑4 (MQ=17)
                                                                                                               gccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAGTATCTAATGTATGTCCCTCTGTATc                             >  2:13534/2‑140 (MQ=11)
                                                                                                               agcTTCGTTGAATGTGCGTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc                             <  1:109667/138‑1 (MQ=16)
                                                                                                                ccTTCGTTGAAGGTGCCTGATCTGTAATTTCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTAAGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                            <  2:202439/140‑1 (MQ=12)
                                                                                                                ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                            >  1:142336/1‑140 (MQ=17)
                                                                                                                ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACACAATATCTAATGTATGTCCCTCTGTATCa                            >  2:199721/1‑140 (MQ=12)
                                                                                                                ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                            <  2:77336/140‑1 (MQ=255)
                                                                                                                  ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTTCCTCTGCCTAAAt                          >  2:287264/1‑140 (MQ=12)
                                                                                                                  ttCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                          <  2:187693/140‑1 (MQ=12)
                                                                                                                    cGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATgg                        >  1:218631/1‑140 (MQ=32)
                                                                                                                    cGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGTTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATgg                        >  1:232805/1‑140 (MQ=11)
                                                                                                                     gTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGTGACTTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATTTATGTCCCTCTGTATCAATGGc                       >  1:61668/1‑140 (MQ=255)
                                                                                                                     aacgAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                      >  1:182402/4‑125 (MQ=17)
                                                                                                                      ttGAAGGTGCCTGATCTTTAATTACCTTTTGAGTTTTACCAAATTTTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCa                      <  1:114649/140‑1 (MQ=12)
                                                                                                                        gAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACg                    <  2:63077/140‑1 (MQ=33)
                                                                                                                          aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATACACGCATATGCTGAATGATTATTTCGTTGCTTGCGCTACCAGATATCTAATGTATGTTCCTCTGTATCAATGGCACgtt                  >  2:59392/1‑138 (MQ=11)
                                                                                                                               ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATTGCACGATATaaa             >  1:117794/1‑140 (MQ=18)
                                                                                                                                  gATCTGTAAGTACCTGTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAg          <  1:8386/140‑1 (MQ=16)
                                                                                                                                   aTCTGTAACTACCTTTTGGGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGc         <  2:48241/140‑1 (MQ=255)
                                                                                                                                    tCTGTAATTAACTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAAACATATGCTGACTGATTATCTCGTAGCTTACGCAACCAAATATCTAATGTCAGTCCGCCTGCGTAATAGGCACGATATAAACAgat        >  2:282700/1‑138 (MQ=11)
                                                                                                                                     cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTc       >  1:288488/1‑140 (MQ=32)
                                                                                                                                     cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTc       <  1:262549/140‑1 (MQ=33)
                                                                                                                                     cTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTc       >  2:115223/1‑140 (MQ=255)
                                                                                                                                     cTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTc       <  1:151493/140‑1 (MQ=255)
                                                                                                                                       gTAATTAACTTTTAAGGGTTACCCAATTAGTTAATGAGACGGTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAATCAAACAATCAATGTTTGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCa     <  1:168844/140‑1 (MQ=11)
                                                                                                                                        tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAt    <  2:118234/140‑1 (MQ=18)
                                                                                                                                          aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCACTGGCGCGATATAAATAGCTCCAttt  >  1:92941/1‑140 (MQ=12)
                                                                                                                                           |                                                                                                                                          
TTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTT  >  CP000730/36022‑36299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: