BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,174	T→C	29.6%	K126K (AAA→AAG)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,174	0	T	C	29.6%	308.8 / 226.3	320	K126K (AAA→AAG)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (107/113); new base C (43/51); total (151/169)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

Only 100 of 332 total aligned reads displayed.

TATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGA  >  CP000730/36035‑36313

tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGt                                                                                                                                             >  1:293546/1‑140 (MQ=19)

taggACGGTTATCTTGCTCAATGAGGTTAATCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGt                                                                                                                                             <  1:228736/137‑1 (MQ=255)

  tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGttt                                                                                                                                           <  2:7814/140‑1 (MQ=25)

    aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                                                                                                         <  2:26390/140‑1 (MQ=25)

    aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTTAGGCTTAc                                                                                                                                         >  1:14710/1‑140 (MQ=11)

     cGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAcc                                                                                                                                        <  1:176530/140‑1 (MQ=11)

       gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaa                                                                                                                                      <  1:198771/140‑1 (MQ=16)

        tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                                     <  2:244050/140‑1 (MQ=17)

        tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaaa                                                                                                                                     <  1:302067/140‑1 (MQ=11)

          aTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGCGGTTTAACAAGtt                                                                                                                                   >  2:254663/1‑140 (MQ=11)

          aTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAAtt                                                                                                                                   >  1:231779/1‑140 (MQ=255)

            cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                                 >  1:184494/1‑140 (MQ=255)

             ttGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCATTCAGGTTAAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGACCTGTAATTACCTCTTGAGGTTTCCCAAATTGtt                                                                                                                                >  1:211844/1‑140 (MQ=11)

             ttGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGtt                                                                                                                                >  2:37273/1‑140 (MQ=16)

             ttGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGtt                                                                                                                                >  1:118234/1‑140 (MQ=18)

               gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTa                                                                                                                              >  2:44723/1‑140 (MQ=16)

                cTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTaa                                                                                                                             <  1:51434/140‑1 (MQ=11)

                 tCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                            <  1:308549/140‑1 (MQ=16)

                 tCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                            <  1:259647/140‑1 (MQ=16)

                   aaTGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATga                                                                                                                          <  1:105073/140‑1 (MQ=32)

                    aTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgag                                                                                                                         >  1:178614/1‑140 (MQ=12)

                    aTGAAGTTATTCAGATATTTCGATGTAAAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgag                                                                                                                         <  2:127207/140‑1 (MQ=255)

                     tGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAGATTGTTTAATgaga                                                                                                                        >  1:41859/1‑140 (MQ=11)

                     tGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATgaga                                                                                                                        >  1:262155/1‑140 (MQ=11)

                         gTTAATCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGCTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                                                                    <  1:216189/140‑1 (MQ=17)

                          ttATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAGCTTCGTTGAAGGTGCCTGATCTGTAATTACCGTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                                                                   <  2:93107/140‑1 (MQ=255)

                          ttATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGCCGttt                                                                                                                   >  2:111344/1‑140 (MQ=255)

                          ttATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGGGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTATTGAGCCGttt                                                                                                                   >  2:174550/1‑140 (MQ=11)

                          ctATTCAGATATTTCGATGTACAATGACAGGCAGGTTTAAGTGTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCACATATATAACCACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGttt                                                                                                                   <  2:1506/139‑1 (MQ=11)

                           tATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACTAAATTGTTTAATGAGACGTTTg                                                                                                                  <  2:233853/140‑1 (MQ=11)

                           tATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                                                                                                                  <  2:284324/140‑1 (MQ=11)

                                 gctatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAc                                                                                                            <  1:39906/138‑1 (MQ=12)

                                 gATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTATAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGCTCTGTAATTACCTTTTGAGGTGTACCAAATTGTTTAATGAGACGTTTGATAAAc                                                                                                            >  2:175775/1‑140 (MQ=11)

                                  atatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTACTGAGACGTTTGATAAACg                                                                                                           >  2:73718/1‑140 (MQ=255)

                                    atTTCTATGTACAATGACAGTCAGGTTTAAGTTTAAAATCTTGCATTTTTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                                                         <  1:246765/140‑1 (MQ=11)

                                     tttCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCat                                                                                                        <  1:299180/140‑1 (MQ=16)

                                     tttCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCat                                                                                                        <  1:167449/140‑1 (MQ=12)

                                      ttCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAAGCata                                                                                                       >  1:189500/1‑140 (MQ=12)

                                       tagATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAACAAATTGTTTAATGAGACGTTTGATAAACGCatat                                                                                                      <  1:265180/138‑1 (MQ=11)

                                        cGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                                                                     <  2:312121/140‑1 (MQ=11)

                                         gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                                                                    >  1:156387/1‑140 (MQ=16)

                                         gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                                                                    <  1:48371/140‑1 (MQ=17)

                                          aTGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACACATATGCt                                                                                                   >  2:252870/1‑140 (MQ=11)

                                              aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAAt                                                                                               <  1:205673/140‑1 (MQ=17)

                                                 aTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                                                            <  2:196341/140‑1 (MQ=32)

                                                   gACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTCACTACCTTTTGAGGCTTACCAAATAGTTTAATGCGACGCTTGATATACGCATACGCCGAATGATTa                                                                                          <  2:267707/140‑1 (MQ=11)

                                                   gACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCATGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGCATGATTa                                                                                          >  2:299041/1‑140 (MQ=11)

                                                                                      ttAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAAACAAATATCGCatgtatg                                                       >  1:66765/1‑140 (MQ=2)

                                                                                       tAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatgt                                                      >  1:283219/1‑140 (MQ=9)

                                                                                          ccATTGCTGACTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTccc                                                   <  1:107697/140‑1 (MQ=17)

                                                                                          ccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTccc                                                   >  1:221824/1‑140 (MQ=32)

                                                                                            aTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                                 >  1:133656/1‑140 (MQ=32)

                                                                                                caacctttctggAAGGTGCCTGAAATGTAATTACCTTTGGAGGTTTAGCAACTTGTTTAATGCGACGTTTGATAAACGAATATATTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                             <  1:75118/129‑1 (MQ=11)

                                                                                                caaCCTTGGTTGAAGGCCGCTGATCTGTACTGACCTTTTGAGGTTTACCAAATTGTTTAAGGATACGTTTGATAAACGCATATGCTTAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                             <  2:140923/138‑1 (MQ=11)

                                                                                                cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgta                                             >  1:303561/1‑138 (MQ=18)

                                                                                                 tgtcTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgtat                                            <  1:157449/137‑4 (MQ=17)

                                                                                                 tACCTTCTGTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAt                                            <  2:212362/140‑1 (MQ=18)

                                                                                                   cgttCGTGGAGGATGCTTGATCTGTAATTCCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                                          <  1:85814/138‑1 (MQ=11)

                                                                                                   ccTTCGTTGAAGGTGCCTGATCTGTAATTTCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTAAGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                          <  2:202439/140‑1 (MQ=12)

                                                                                                   ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                          <  2:107832/140‑1 (MQ=18)

                                                                                                    cTTCGTTGAAGGGGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                         <  1:33902/140‑1 (MQ=16)

                                                                                                     ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATCATCTCGTTGCTTACGCAACCAAATTCCCAATGTATGTCCCTCTGTATCAAt                                        <  2:227074/140‑1 (MQ=11)

                                                                                                      tggTTGAAGGTGGGTGTTCTGTGATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGCATGACTATCCCGTTGTTCACCCAACCAAATCTCTAATGTATGTCCCGCTGTATCAATg                                       <  2:160037/138‑1 (MQ=11)

                                                                                                      tCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTCTGTCCCTCTGCCTCTATg                                       >  1:27888/1‑140 (MQ=12)

                                                                                                       cGTTGAAGGTGCCTGATCTGTATTTACCTTTTGAGGTTTACCAAATTGTTTGCAATTTGGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAACTATCTAATGTATGTCCCTCTGCATCAATgg                                      <  1:313803/140‑1 (MQ=11)

                                                                                                        gTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGTGACTTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATTTATGTCCCTCTGTATCAATGGc                                     >  1:61668/1‑140 (MQ=255)

                                                                                                        gTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCCACCAAATATCTAATGTATGTCCCTCTGCCTCACTGGc                                     >  2:216914/1‑140 (MQ=12)

                                                                                                        aacgAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                                    <  2:182402/122‑1 (MQ=18)

                                                                                                        aacgAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGc                                     >  2:275101/4‑140 (MQ=17)

                                                                                                         ttGAAGGTGCCTGATCTTTAATTACCTTTTGAGTTTTACCAAATTTTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCa                                    <  1:114649/140‑1 (MQ=12)

                                                                                                         ttGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCa                                    >  1:285230/1‑140 (MQ=17)

                                                                                                           gAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACg                                  <  2:63077/140‑1 (MQ=33)

                                                                                                             aGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATATAATGTATGTCCCTCTGTATCAATGGCACGat                                >  2:117853/1‑140 (MQ=11)

                                                                                                              ggTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGata                               <  2:87373/140‑1 (MQ=18)

                                                                                                                tGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGAtata                             <  2:5329/140‑1 (MQ=33)

                                                                                                                  ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATaaa                           >  1:253768/1‑140 (MQ=32)

                                                                                                                    tGATCTGTAAGTACCTTTTGAGGATTACCAAATTGTTTAATGAGACGTTTGTTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATTAATa                         <  1:217872/140‑1 (MQ=11)

                                                                                                                       tCTGTAATTAACTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAAACATATGCTGACTGATTATCTCGTAGCTTACGCAACCAAATATCTAATGTCAGTCCGCCTGCGTAATAGGCACGATATAAACAgat                      >  2:282700/1‑138 (MQ=11)

                                                                                                                       tCTGTAATGACCTTTTGAGGGTTACCAAATTGTTTAATGAGACGTTTGATAACCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCt                      <  2:18653/140‑1 (MQ=12)

                                                                                                                        cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCCACCAAATGTCTACTGTCTGTCCCTCTGCCTCAGTGGCACGATGTAGATAGCTc                     >  1:23320/1‑140 (MQ=11)

                                                                                                                        cTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTc                     >  2:115223/1‑140 (MQ=255)

                                                                                                                         tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTcc                    <  1:15178/140‑1 (MQ=33)

                                                                                                                         tGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTGTCAATGGCACGATATAAATAGGTcc                    >  2:59876/1‑140 (MQ=11)

                                                                                                                         tGTAACTAACTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTcc                    <  1:145451/140‑1 (MQ=11)

                                                                                                                          gtactcaCCTTTTGAGGTTTACCAAAGTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCa                   <  2:204534/134‑1 (MQ=16)

                                                                                                                            aaTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTCTCAATGGCCCGATATAAATAGCTCCAtt                 >  1:95348/1‑140 (MQ=12)

                                                                                                                             attcgcgtttGAGGTTTACCAAATTGTTTAAGGAGACGATTAATAAACGCAAATGCTGAATGTTTATCTCGTTGCTTACGCCACCTAATATCTAAACCCAGTCCCTATGCATCAATGGCACGCTATAAATAGCTCCAttt                >  2:134601/8‑140 (MQ=11)

                                                                                                                             aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATTCTGAATGATTATCTCGTTGCTTACGCAACAAAATATCTAATGTATGTCCCTCTGCATCAATGACACGATATAAATAGCTCCAttt                >  1:80937/1‑140 (MQ=12)

                                                                                                                             aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCACTGGCGCGATATAAATAGCTCCAttt                >  1:92941/1‑140 (MQ=12)

                                                                                                                              ctACCTCTGGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTCAATGATTATCTCGTTGCTTACGCTACCAAATATCTAATGTATGTCACTCCGTACCAATGGCACGATATAAATAGCTCCAtttt               <  2:119421/139‑1 (MQ=11)

                                                                                                                                aCCTTTTTAGGTTCACCAAATTGTTTAATGAGACGTTTGATAAACTCATACGCTGAATGATTACCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTAAAACAATTTCACCATTTAAATAGCGCCATTTTcc             <  2:133289/140‑1 (MQ=11)

                                                                                                                                aCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATCTGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTcc             >  2:156717/1‑140 (MQ=17)

                                                                                                                                aCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTcc             <  2:163542/140‑1 (MQ=18)

                                                                                                                                  cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATAGCCCCATATCAATAGCTCCATTTTCCtt           >  2:144650/1‑140 (MQ=255)

                                                                                                                                    tttGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtttt         <  2:200977/140‑1 (MQ=16)

                                                                                                                                    tttGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtttt         >  1:220550/1‑140 (MQ=12)

                                                                                                                                     ttGAGGTTTACCAAATTGTTTCCTGCGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCAATTTTCCttttc        >  2:147423/1‑139 (MQ=255)

                                                                                                                                     ttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCACTTTTCCttttc        >  2:281667/1‑139 (MQ=16)

                                                                                                                                      tGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAt       >  2:315048/1‑140 (MQ=18)

                                                                                                                                         ggTTTTCCAAATTGTTTAATGAGCCGTTTGATAACCGCATATGCTGAAGGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtttt    <  1:271285/140‑1 (MQ=255)

TATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGA  >  CP000730/36035‑36313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`