Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,269 C→T 56.8% A95T (GCA→ACA)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,2690CT56.8% 64.7 / inf 375A95T (GCA→ACA) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (74/86);  new base T (91/119);  total (166/209)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 378 total aligned reads displayed.

CTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGG  >  CP000730/36131‑36408
                                                                                                                                           |                                                                                                                                           
caacctttctggAAGGTGCCTGAAATGTAATTACCTTTGGAGGTTTAGCAACTTGTTTAATGCGACGTTTGATAAACGAATATATTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                                                                                                                            <  1:75118/129‑1 (MQ=11)
caaCCTTCGGTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                                                                                                                            <  1:155918/138‑1 (MQ=18)
 tACCTTCTGTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAt                                                                                                                                           <  2:212362/140‑1 (MQ=18)
  gtcTTCGTTGAATGTGCCTGATCTGTAACTACCTGTTGGGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                                                                                                                                          <  1:50968/138‑1 (MQ=11)
  gccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTACCTCTGTATc                                                                                                                                          >  2:13422/2‑140 (MQ=11)
   ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                                                                                                                         >  1:142336/1‑140 (MQ=17)
   ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACACAATATCTAATGTATGTCCCTCTGTATCa                                                                                                                                         >  2:199721/1‑140 (MQ=12)
   acTTCCTTGAAGGTGCCTGATCTGTAATTACCTTTGGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATACGCTGACTCCTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                                                                                                                                         <  2:159726/139‑1 (MQ=11)
      tctttGAAGGTGCCTGATGTGTAACTACCTTTTGAGTCTTACCAAATTGTTTAATGCGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                                                                                                                                      <  1:106326/137‑1 (MQ=11)
      tCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTCTGTCCCTCTGCCTCTATg                                                                                                                                      >  1:27888/1‑140 (MQ=12)
             aGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATATAATGTATGTCCCTCTGTATCAATGGCACGat                                                                                                                               >  2:117853/1‑140 (MQ=11)
                  ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATaaa                                                                                                                          >  1:253768/1‑140 (MQ=32)
                   cTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTACTGTCTGTCCCTCTGTATCAATGGCACGATATAAAt                                                                                                                         <  2:275125/140‑1 (MQ=11)
                       tCTGTAATTAACTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAAACATATGCTGACTGATTATCTCGTAGCTTACGCAACCAAATATCTAATGTCAGTCCGCCTGCGTAATAGGCACGATATAAACAgat                                                                                                                     >  2:282700/1‑138 (MQ=11)
                        cggTAAATACCTTTTGAGGCTTACCAAATTGTTTACTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTc                                                                                                                    <  1:257187/138‑1 (MQ=11)
                        cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCCACCAAATGTCTACTGTCTGTCCCTCTGCCTCAGTGGCACGATGTAGATAGCTc                                                                                                                    >  1:23320/1‑140 (MQ=11)
                        cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTc                                                                                                                    >  1:288488/1‑140 (MQ=32)
                        cTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTc                                                                                                                    >  2:115223/1‑140 (MQ=255)
                         tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTcc                                                                                                                   <  1:15178/140‑1 (MQ=33)
                         tGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTGTCAATGGCACGATATAAATAGGTcc                                                                                                                   >  2:59876/1‑140 (MQ=11)
                          gtactcaCCTTTTGAGGTTTACCAAAGTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCa                                                                                                                  <  2:204534/134‑1 (MQ=16)
                             aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCACTGGCGCGATATAAATAGCTCCAttt                                                                                                               >  1:92941/1‑140 (MQ=12)
                              ctACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGCATGATTATCTCGTTGCTTAAGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGTACGATCTTAATAGCTcccattt                                                                                                              >  2:11931/2‑135 (MQ=11)
                              ctACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTACTGTTTTGTCCACCTTATCAATGGCACGATATAAATAGCTCCAtttt                                                                                                              >  1:298320/2‑140 (MQ=11)
                               tACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTCGATCAACGACACGATAGAAAAAACGCCAtgtcc                                                                                                             >  1:305957/1‑136 (MQ=11)
                               tACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTc                                                                                                             <  2:222163/140‑1 (MQ=16)
                                aCCTTTTTAGGTTCACCAAATTGTTTAATGAGACGTTTGATAAACTCATACGCTGAATGATTACCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTAAAACAATTTCACCATTTAAATAGCGCCATTTTcc                                                                                                            <  2:133289/140‑1 (MQ=11)
                                aCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTcc                                                                                                            <  2:163542/140‑1 (MQ=18)
                                aCCTTTTGAGGGTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTcc                                                                                                            <  2:125235/140‑1 (MQ=12)
                                   ttttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                         >  2:129899/1‑140 (MQ=18)
                                    tttGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtttt                                                                                                        >  1:220550/1‑140 (MQ=12)
                                    tttGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtttt                                                                                                        <  2:13529/140‑1 (MQ=12)
                                     ttGAGGTTTACCAAATTGTTTCCTGCGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCAATTTTCCttttc                                                                                                       >  2:147423/1‑139 (MQ=255)
                                      tGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAt                                                                                                      >  2:315048/1‑140 (MQ=18)
                                       ggggTTTACCAAATTTTTTAATGAGACGTGTGATAAACGCATCTGCTGAATGATTATCTCGGTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt                                                                                                     <  1:42267/138‑1 (MQ=11)
                                       gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACTCAACCAAATATCTAATGTATGTCCCTCTGTATCTATTGCACGATATAATTAGTGCCATTTTCCTTTTAtt                                                                                                     >  1:264750/1‑140 (MQ=11)
                                       gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATCTACCTTTTGtt                                                                                                     <  2:196664/140‑1 (MQ=255)
                                          gcttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg                                                                                                  >  1:301610/3‑140 (MQ=18)
                                          gcttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg                                                                                                  >  1:70223/3‑140 (MQ=17)
                                           tttAACAAATTGGTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGa                                                                                                 <  1:178399/140‑1 (MQ=18)
                                            ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAt                                                                                                <  1:262359/140‑1 (MQ=18)
                                              aCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt                                                                                                     >  2:252547/1‑133 (MQ=18)
                                              aCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtat                                                                                              <  2:100470/140‑3 (MQ=17)
                                               ccAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAATTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTa                                                                                             >  1:100747/1‑140 (MQ=17)
                                                 aaaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACg                                                                                           >  1:286025/1‑140 (MQ=32)
                                                   tttggtcaaTGCGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGtc                                                                                         <  1:115626/133‑1 (MQ=255)
                                                   aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtc                                                                                         >  1:274277/1‑140 (MQ=18)
                                                   aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtc                                                                                         >  2:39508/1‑140 (MQ=12)
                                                   aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCCCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtc                                                                                         >  2:37106/1‑140 (MQ=255)
                                                    ttttgtAATGAGACGTTTGATAAACGCCTTTGCTTAATGATTATCTCGTTGCTTACGCAACCAAATAGCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGtct                                                                                        <  1:148362/135‑1 (MQ=11)
                                                       tttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                     <  2:298481/140‑1 (MQ=33)
                                                         tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcttca                                                                                   >  1:284098/1‑140 (MQ=255)
                                                         tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcatca                                                                                   <  1:124302/140‑1 (MQ=17)
                                                           aggcgagctttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                 <  2:149169/132‑1 (MQ=11)
                                                           aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                 <  1:240284/140‑1 (MQ=33)
                                                            tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATa                                                                                <  2:94188/140‑1 (MQ=12)
                                                                  gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCCTCAATACGCCAt                                                                          >  2:261418/1‑140 (MQ=17)
                                                                     tGATAAACGCATATGCTGAATGATTAGCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTg                                                                       >  2:191528/1‑140 (MQ=17)
                                                                          aaCGCATATTCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaata                                                                  <  2:54164/140‑1 (MQ=255)
                                                                          aaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaata                                                                  <  1:79525/140‑1 (MQ=33)
                                                                           aCGCATATTTTGAATCATTATCTCGTTGCTTACGCAACTAAATTTCTAATGTCTCTCCCTCTGTATCCATGGCACGATATATGTAGCTCCATTTTCCTTTTATTTTGCTATACTTCTCATCAATACGCCATTTGtaataa                                                                 <  2:278752/140‑1 (MQ=11)
                                                                           aCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaataa                                                                 >  1:224839/1‑140 (MQ=34)
                                                                            cGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAg                                                                >  2:140348/1‑140 (MQ=19)
                                                                             gCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGc                                                               <  2:237261/140‑1 (MQ=255)
                                                                               atatGATGAATGATTATCTCGTTACTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATAAACGTCTCATCAATAAGCTATTTGTCATAAACtt                                                             >  2:271117/1‑140 (MQ=11)
                                                                                  tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCttatg                                                          >  2:184937/1‑137 (MQ=25)
                                                                                  agCTGAACGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCttttt                                                          <  1:162512/139‑1 (MQ=37)
                                                                                    cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTa                                                        >  2:240477/1‑140 (MQ=255)
                                                                                      gAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATg                                                      <  2:302088/140‑1 (MQ=255)
                                                                                        aTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCt                                                                                                           <  2:155617/85‑1 (MQ=25)
                                                                                             tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATTGCTCCATTTTCTTTTTATTTTGATGTACGTCTCATCATTATGTCATTTTTAATAAGGTTTTTTATGCTTTttc                                               >  1:19830/1‑140 (MQ=14)
                                                                                               tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATATCTCCTTTTTCCTTTTATTTTGATTTATGTCTTATCTATACGCCATCTGTAATCAGCTTTTTTATGCTGtttttt                                             >  1:8523/1‑140 (MQ=11)
                                                                                                 tcGTTGCTTACGCCACCCACTATCTAATGGATGTCTCCCTGGATCCATGGCGCGTTATCCATAGCTCCATTTTCCTTTTCTTTGGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                           <  2:64888/140‑1 (MQ=14)
                                                                                                 ccattGCTTACGGAACTAAATATCTAATGTATGTCCCTCTTCATTACTGGCTCTATATAAATATCTCCTTTTTCCTTTTTTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                           <  2:81440/137‑1 (MQ=14)
                                                                                                  cGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCAATTTTTATTAAGCTTTTTTATGCTTTTTTTTCCa                                          >  1:53703/1‑140 (MQ=255)
                                                                                                    tttcTTACGCAACCAAATATTTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCaaa                                        <  2:211349/137‑1 (MQ=255)
                                                                                                       cTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAttt                                     >  1:255342/1‑140 (MQ=40)
                                                                                                           cGCAACGAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATa                                 >  2:201747/1‑140 (MQ=255)
                                                                                                            gccaCCAAATATCTAATGTATTTCCCTCTGTATAAATGGCACGATATAAATAGCTCCATTTTCCGTTTATTTTGATTTACGTCGCATCAATACGCCATTTGTAATAAGCTTTTTTAGGCTTTTTCTTCCAAATTTGATat                                <  2:73580/137‑2 (MQ=11)
                                                                                                            gCAACCAAACATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                <  1:11931/140‑2 (MQ=255)
                                                                                                             cAACCAATTATCTAATGTCTGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                               <  1:182470/140‑3 (MQ=11)
                                                                                                             cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                               <  1:173314/140‑3 (MQ=255)
                                                                                                             cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACATCTCATCAATACGCCGTTTGTAATAAGCTTTTTTATGGTTTTTCTTCCAAATTCGATatc                               >  1:131228/1‑138 (MQ=14)
                                                                                                               aCCAAATATCCAATGTATGTCCCTCTGCATCCATGGCCAGCTATCCATCGGTGCTTATCGCATTTAGGTTGAAGTAAGTATCATCATTTCGCCAGTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaa                             <  2:306941/140‑1 (MQ=2)
                                                                                                                 cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAAATAAAAt                           >  1:96061/1‑140 (MQ=255)
                                                                                                                 cAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCCTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTTCAAATTTGATATAAAAt                           >  2:8938/1‑140 (MQ=255)
                                                                                                                   aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCCAATTTGATATAAAATTg                         >  2:143023/1‑140 (MQ=255)
                                                                                                                   aaTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTTATCAATACGCCTTTTGTTATAAGCTTTTTTATCCTTTTTCTTCTTATTTTGATatcagtttg                         >  1:112812/1‑132 (MQ=14)
                                                                                                                    atatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCTAAATTTGATACAAAATTgg                        <  1:191528/140‑1 (MQ=255)
                                                                                                                     tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTggg                       <  1:60444/140‑1 (MQ=255)
                                                                                                                     tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTGCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAACAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTggg                       <  2:176516/140‑1 (MQ=255)
                                                                                                                         tAATGTTTGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                   <  1:282700/140‑1 (MQ=255)
                                                                                                                         tAATCTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                   <  2:232825/140‑1 (MQ=255)
                                                                                                                                 tgtCCTTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg            <  1:247539/140‑1 (MQ=255)
                                                                                                                                 tgtCACTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg            <  1:23064/140‑1 (MQ=255)
                                                                                                                                  gtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa           <  1:316122/140‑1 (MQ=21)
                                                                                                                                   tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACAATTGGGGCATATTCTTGaa          >  2:286318/1‑140 (MQ=255)
                                                                                                                                   tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa          >  2:49739/1‑140 (MQ=255)
                                                                                                                                   tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa          >  2:22903/1‑140 (MQ=255)
                                                                                                                                      ctctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAccc       <  1:202569/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGG  >  CP000730/36131‑36408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: