Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,315 G→A 57.8% Y79Y (TAC→TAT)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,3150GA57.8% 123.6 / inf 404Y79Y (TAC→TAT) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base G (76/89);  new base A (113/116);  total (197/207)
Fisher's exact test for biased strand distribution p-value = 5.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.27e-01

Only 100 of 407 total aligned reads displayed.

CCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTT  >  CP000730/36178‑36454
                                                                                                                                          |                                                                                                                                           
ccAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTG‑CTTACGCAACCAATTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTa                                                                                                                                           >  1:100747/1‑140 (MQ=17)
 cAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATAc                                                                                                                                          <  2:217842/140‑1 (MQ=16)
 cAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGACACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGAAc                                                                                                                                          >  2:120809/1‑140 (MQ=17)
  aaaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACg                                                                                                                                         >  1:286025/1‑140 (MQ=32)
    gttGTTTAAGGAGACGTTTGATAAACGCATATGCTGAATGATTATATCTTTC‑TTTACTCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtc                                                                                                                                       <  1:248124/139‑1 (MQ=11)
    aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTG‑CTTACTCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGtc                                                                                                                                       >  1:86624/1‑140 (MQ=17)
    aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGAACCTCCACATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGtc                                                                                                                                       <  1:299863/140‑1 (MQ=255)
     ttttgtAATGAGACGTTTGATAAACGCCTTTGCTTAATGATTATCTCGTTG‑CTTACGCAACCAAATAGCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGtct                                                                                                                                      <  1:148362/135‑1 (MQ=11)
        tttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                                                                   <  1:275117/140‑1 (MQ=33)
            aggcgagctttGATAAACGCATATGCTGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                                                               <  2:149169/132‑1 (MQ=11)
            aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                                                               <  2:282531/140‑1 (MQ=32)
              gagaaatttGATAACCGCACATGCTGCATGATCATCTACTTGAAATAGTCAA‑AAAAAGTCTGATGTATGTCCCTCTGCAGCTATGGCACGATATTAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATAc                                                                                                                             <  2:67383/134‑1 (MQ=2)
                      tGATAAACGCATATGCTGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCTTCAATAAGCCATTTg                                                                                                                     >  2:129023/1‑140 (MQ=17)
                         tcaaCGCATATGCTGAATGATTATCTCGTTG‑CTTACGCAACCAAACATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaa                                                                                                                  <  1:25667/138‑1 (MQ=17)
                          aaaCGCATATGCTGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaat                                                                                                                 <  1:115223/140‑1 (MQ=12)
                           aaCGCATATGCTGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaata                                                                                                                <  2:231573/140‑1 (MQ=16)
                            aCGCATATGCTGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaataa                                                                                                               >  1:224839/1‑140 (MQ=34)
                                   tGCTGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCCTCAATACGCCATTTGTAATAAGCttttt                                                                                                        >  1:122668/1‑140 (MQ=255)
                                    gCTGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATTTTCGTCTCATCAAGACGCCATTTGTAATAAGCtttttt                                                                                                       >  1:19686/1‑140 (MQ=255)
                                     cTGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTa                                                                                                      >  2:240477/1‑140 (MQ=255)
                                     cTGAATGATAATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTATATAAATTGCATGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCGTTTTTa                                                                                                      >  1:233874/1‑140 (MQ=11)
                                      tGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAt                                                                                                     <  2:117794/140‑1 (MQ=255)
                                        aaTGATTATCTCGGTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATCTTCCTTTGATGTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGc                                                                                                   <  2:23320/140‑1 (MQ=255)
                                          tGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCATTTTATTTTGATGTAAGTCTCATAAATACGCCATTTGTAATAATCTTTTTTATGCtt                                                                                                 >  2:31582/1‑140 (MQ=255)
                                            aTTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGCTATAAATAGCTCCATTTTCATTTTATTTTGATATACGACCCATGAATAAGCCATTTGTAATAAGCTTTTTTATGCattt                                                                                               >  2:133908/1‑140 (MQ=14)
                                             ttATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAAACGCCATTTGTAATAAACTTTTTTATGCttttt                                                                                              >  2:255095/1‑140 (MQ=255)
                                             ttATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTCTATTTACTTATTATCAATAAGCAATTTGTAATAAGCTTTTTTATGCttttt                                                                                              >  1:151171/1‑140 (MQ=14)
                                             ttATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTCATCAATACGCCCTTTGTAATAAGCTTTTTTATGCttttt                                                                                              >  2:207486/1‑140 (MQ=255)
                                                tctcGTTG‑CTTACGCAACAAAATATCTAATGTATGTTCTTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                                                                           <  1:278824/140‑1 (MQ=14)
                                                 ctcGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                          <  1:64103/140‑1 (MQ=255)
                                                 ctcGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                          <  2:277841/140‑1 (MQ=255)
                                                  tcGGTG‑CTTACGATACCCAATTTCGAGTGTCGGTCTCACTGCATCAGTGGTACGATATAAATAGCTCCATTTTGCTTTTATTTTGATGTCCGTCTCATCAATACGCGATTTGTCATGAGCTTTTTTATGCtttttatacc                                                                                         <  2:211844/140‑6 (MQ=2)
                                                  ccattG‑CTTACGGAACTAAATATCTAATGTATGTCCCTCTTCATTACTGGCTCTATATAAATATCTCCTTTTTCCTTTTTTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                                                                         <  2:81440/137‑1 (MQ=14)
                                                       g‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATGATTACGCCATTTGTAACAATCTTTTTTATGCTTTTTCTTCCAAAtt                                                                                    >  1:130678/1‑140 (MQ=255)
                                                           tACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGa                                                                                 >  2:140248/1‑140 (MQ=255)
                                                             cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATa                                                                               <  2:195332/140‑1 (MQ=255)
                                                              gccaCCAAATATCTAATGTATTTCCCTCTGTATAAATGGCACGATATAAATAGCTCCATTTTCCGTTTATTTTGATTTACGTCGCATCAATACGCCATTTGTAATAAGCTTTTTTAGGCTTTTTCTTCCAAATTTGATat                                                                              <  2:73580/137‑2 (MQ=11)
                                                              gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAACCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                                                              >  1:53804/1‑139 (MQ=255)
                                                                 aCCAAATATCCAATGTATGTCCCTCTGCATCCATGGCCAGCTATCCATCGGTGCTTATCGCATTTAGGTTGAAGTAAGTATCATCATTTCGCCAGTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaa                                                                           <  2:306941/140‑1 (MQ=2)
                                                                  ccAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTACTAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATTTaaaa                                                                          >  2:91139/1‑140 (MQ=255)
                                                                      atatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                      >  1:238662/1‑140 (MQ=38)
                                                                      atatCTAATGTATGTACCTCTGTATCAATGGCACGATATAAATAGCTCAATATTCCTTTTATATTGATATACGTCTCATCAATACGCCATGTGTAATAAGCTTTTTTATGATTTTTCTTCCAGATTTGATATAAAATTgc                                                                      >  2:271086/1‑139 (MQ=11)
                                                                       tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTggg                                                                     <  1:60444/140‑1 (MQ=255)
                                                                           tAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGTTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                 <  1:76042/140‑1 (MQ=11)
                                                                             atgtatgtTCCTCTTTATTAATGGCACTATATAAATAGCTCCTTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCatat                                                               <  1:108493/140‑1 (MQ=11)
                                                                               gtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTc                                                             <  1:260943/140‑1 (MQ=255)
                                                                                  tgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGAAAATTCTTg                                                          >  1:97946/1‑140 (MQ=255)
                                                                                  tgtCACTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                          <  1:23064/140‑1 (MQ=255)
                                                                                   gtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa                                                         <  1:316122/140‑1 (MQ=21)
                                                                                        tttgtattaaTGGCACCATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCa                                                    <  2:262155/132‑1 (MQ=11)
                                                                                          tgtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc                                                  >  2:302974/4‑140 (MQ=255)
                                                                                          tGCATCAATGGCACAATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc                                                  <  1:205710/140‑1 (MQ=16)
                                                                                           gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACg                                                 <  2:29890/138‑1 (MQ=255)
                                                                                            taTCAATGGCACGATATTAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                <  2:95275/139‑1 (MQ=255)
                                                                                            cATCAATGTCTCGATATAATTAGCTCCTTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                <  1:25400/140‑1 (MQ=11)
                                                                                            cATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTCTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAACATTTTTTATGCTTTTTCTTACAAATATGATATAAAATTGCGGCAAATTCTTGAAGCCATCgg                                                >  2:274205/1‑140 (MQ=11)
                                                                                               cAATGGCACGTTATAATTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                             <  1:26614/140‑1 (MQ=255)
                                                                                                taTGGCACGATATAAATATCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                            <  2:88053/139‑1 (MQ=19)
                                                                                                caTGGCACTATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAGTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                            <  1:140561/139‑1 (MQ=11)
                                                                                                aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCAAACGGCAgc                                            >  2:63726/1‑139 (MQ=255)
                                                                                                aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTGCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                            >  1:200319/1‑140 (MQ=255)
                                                                                                    gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                        >  1:215182/1‑140 (MQ=255)
                                                                                                        gATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGTCTTGTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGaa                                    <  1:283115/140‑1 (MQ=255)
                                                                                                          tataAATGGATACCTTTTCCTTTTATTTTGATATACTTCTCATCACTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCTAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                  <  1:136972/140‑1 (MQ=11)
                                                                                                          tataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCATGAACCCAACGGTAGACCGTTGAatg                                  >  1:243220/1‑140 (MQ=25)
                                                                                                          tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTTAatg                                  >  2:24862/1‑140 (MQ=255)
                                                                                                          tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                  <  1:39508/140‑1 (MQ=255)
                                                                                                           ataAATGCCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                 <  2:181701/140‑1 (MQ=11)
                                                                                                           ataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                 >  1:94594/1‑140 (MQ=255)
                                                                                                           ataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                 <  2:49912/140‑1 (MQ=255)
                                                                                                              aaTAGCTCCCTTCTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAAtgatga                              >  2:70228/1‑140 (MQ=255)
                                                                                                              aaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAAtgatga                              <  1:307146/140‑1 (MQ=40)
                                                                                                              aaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGGATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                              >  2:53692/1‑140 (MQ=11)
                                                                                                               aTAGCGCCATGTTCCTTTTATTTTTATATACGTCTCATCAATACGTCATGTGGAAGAAGCTTTTGTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                             <  2:37586/140‑1 (MQ=11)
                                                                                                                    tCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGttt                        <  1:13422/140‑1 (MQ=255)
                                                                                                                      cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTac                      >  1:125754/1‑140 (MQ=25)
                                                                                                                       aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTaca                     <  1:34144/140‑1 (MQ=40)
                                                                                                                       aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                     <  1:171433/140‑1 (MQ=255)
                                                                                                                        ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                    <  1:44723/140‑1 (MQ=21)
                                                                                                                        ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCGTTGTTATGCTTGTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTTAATGATGCACCTTTacac                    >  2:28983/1‑140 (MQ=11)
                                                                                                                        ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATGTTCTTGAACCCAACGGTATATCGTCGAATGATGAACGTTTacac                    <  1:175691/140‑1 (MQ=11)
                                                                                                                        ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaccc                    <  2:228204/140‑3 (MQ=255)
                                                                                                                         tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATCAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                   <  2:189500/140‑1 (MQ=255)
                                                                                                                          ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCGTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCCTATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                  >  2:42945/1‑140 (MQ=255)
                                                                                                                          ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGCACGTTTAcaccc                  >  1:290893/1‑139 (MQ=11)
                                                                                                                          ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                  >  1:162224/1‑140 (MQ=255)
                                                                                                                           tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                 <  2:285230/140‑1 (MQ=255)
                                                                                                                            ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                <  2:178614/140‑1 (MQ=255)
                                                                                                                            ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                >  2:59969/1‑140 (MQ=255)
                                                                                                                             cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACga               >  2:3994/1‑139 (MQ=255)
                                                                                                                              ttttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt              <  1:186523/140‑1 (MQ=18)
                                                                                                                                ttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTct            <  1:117853/140‑2 (MQ=255)
                                                                                                                                   ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt         <  1:70228/140‑1 (MQ=21)
                                                                                                                                   ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt         >  2:199742/1‑140 (MQ=40)
                                                                                                                                   ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt         <  2:146583/140‑1 (MQ=11)
                                                                                                                                     gtGATATCCGTCTCCGCAATACGCCAGTTGTAATAAGCTTTTGTATGCTTTTTCTTCCAAATTTGATATAAAATTGTGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa       <  1:50778/139‑1 (MQ=11)
                                                                                                                                      tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGATCCCTTAat      >  2:10761/1‑140 (MQ=255)
                                                                                                                                       gATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGATCCTAACGGTAGGTCGTTGAATGATGAGCGCTTTCTCCCCGTTCTCTTAata     >  1:187857/1‑140 (MQ=11)
                                                                                                                                        aTGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat    >  2:158796/1‑140 (MQ=40)
                                                                                                                                          gTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAttt  >  1:295702/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTG‑CTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTT  >  CP000730/36178‑36454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑