Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,378 C→T 88.7% L58L (TTG→TTA)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,3780CT88.7% 639.9 / 70.6 353L58L (TTG→TTA) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (24/16);  new base T (170/139);  total (198/155)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 353 total aligned reads displayed.

ACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGA  >  CP000730/36242‑36511
                                                                                                                                        |                                                                                                                                      
 ccAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTACTAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATTTaaaa                                                                                                                                    >  2:91139/1‑140 (MQ=255)
  cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACAAt                                                                                                                                   >  2:156632/1‑140 (MQ=255)
  cAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTTTGTACGTCTCATTAATACGCCATTTGTAATTAGCTTTTTTATGCATTTTCTTCCAAATTTGATAGAAAAt                                                                                                                                   >  1:266778/1‑140 (MQ=11)
     atatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTg‑g                                                                                                                               >  1:238662/1‑140 (MQ=38)
      tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTg‑gg                                                                                                                              <  1:111344/140‑1 (MQ=255)
      tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTGCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAACAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTg‑gg                                                                                                                              <  2:176516/140‑1 (MQ=255)
         cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GTGCa                                                                                                                           >  1:148679/1‑140 (MQ=255)
           aaTGTAGGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCata                                                                                                                         <  2:74334/140‑1 (MQ=11)
                  gtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGa                                                                                                                  <  1:316122/140‑1 (MQ=21)
                   tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACAATTG‑GGGCATATTCTTGaa                                                                                                                 >  2:286318/1‑140 (MQ=255)
                   tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GTGCATATTCTTGaa                                                                                                                 >  2:261370/1‑140 (MQ=11)
                   tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGaa                                                                                                                 >  2:269626/1‑140 (MQ=255)
                   tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTGTTTTATGCTTTTTCTTCCAAATTTTATATAAAATTG‑GGGCATATTCTTGaa                                                                                                                 >  2:38671/1‑140 (MQ=11)
                      ctctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAAccc                                                                                                              <  1:202569/140‑1 (MQ=255)
                      ctctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAAccc                                                                                                              <  1:134993/140‑1 (MQ=25)
                        ctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCTAATTTGATATAAAATTG‑GGGCATATTGTTGAATCCat                                                                                                            >  1:287565/1‑139 (MQ=11)
                         tgtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCCAc                                                                                                           >  1:176432/4‑140 (MQ=255)
                         tgtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAAc                                                                                                           <  1:37273/137‑1 (MQ=255)
                         tGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATATAATTG‑GGGTATATTCTTGAACCCAAc                                                                                                           <  2:198990/140‑1 (MQ=255)
                          gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACg                                                                                                          >  2:216100/3‑140 (MQ=255)
                          gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGAATATTCTTGAACCCAACg                                                                                                          >  2:110124/3‑140 (MQ=255)
                          gCATCAATGGCACGATATAAATAGCGCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATGCGACATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAATTTTGCTATAAACTTG‑GGGCATATTGTTGAACCCAACg                                                                                                          >  1:193569/1‑140 (MQ=11)
                               aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTGCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGa                                                                                                     >  1:200319/1‑140 (MQ=255)
                                aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGat                                                                                                    <  1:248790/140‑2 (MQ=16)
                                  ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAATCCAACGGTAGATCg                                                                                                  >  2:273975/1‑140 (MQ=11)
                                   gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAGAGAAAATTG‑GGGGATATTCTTTAACCCAACGGTAGAccct                                                                                                 >  1:59328/1‑138 (MQ=11)
                                   gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGt                                                                                                 >  1:215182/1‑140 (MQ=255)
                                   gCACGATATAAATACCTCCCTTTTCCTTTTATGTTTATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCgg                                                                                                 <  2:65723/140‑2 (MQ=11)
                                    cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGatgctg                                                                                                >  2:244073/1‑135 (MQ=255)
                                     aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCGTATTCTTGACCCCAACGGTAGATCGttt                                                                                               >  1:83956/1‑139 (MQ=11)
                                     aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTATAAAAGTATCAAAATAAACTAG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTg                                                                                               >  1:312817/1‑140 (MQ=11)
                                      cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTGGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCAGATTCTTGAACCCAACGGTAGACCGTTGa                                                                                              >  1:63854/1‑140 (MQ=255)
                                         tattAATAGCTCAATTTTCTTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                           <  2:41806/140‑1 (MQ=11)
                                         tataAATAGCTCCATTTTCCTTTTCTTTTGATATACGTCTCATCAATACGCAATTTGTAAGAGGCTTTTTTATTCTTTTTCTTCCAAATTTGATATAAAATTT‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                           >  1:61080/1‑140 (MQ=11)
                                         tataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCATGAACCCAACGGTAGACCGTTGAatg                                                                                           >  1:243220/1‑140 (MQ=25)
                                         tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTCTAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGtttgatg                                                                                           >  2:118598/1‑135 (MQ=11)
                                         tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTTAatg                                                                                           >  2:24862/1‑140 (MQ=255)
                                         tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                           <  2:284098/140‑1 (MQ=255)
                                          ataAATAGCTCCATTTTTCTTTTATTTTTATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                          <  1:252870/140‑1 (MQ=11)
                                            aaatagcctctatttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgagg                                                                                        <  2:293546/128‑3 (MQ=11)
                                            aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTTAatgatg                                                                                        >  2:20775/1‑140 (MQ=255)
                                             aaTAGCTCCCTTCTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAAtgatga                                                                                       >  2:70228/1‑140 (MQ=255)
                                             aaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAAtgatga                                                                                       <  1:307146/140‑1 (MQ=40)
                                              aTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                                                                                      <  2:96325/140‑1 (MQ=255)
                                                 gCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGt                                                                                   <  2:221824/140‑1 (MQ=25)
                                                   tCCATCGTCCTTTTATTTTGCTATAGGACACATCAATACGACATTTGTAATAAGCTTTTTTATGCTGTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGttt                                                                                 >  1:235297/1‑140 (MQ=11)
                                                    ccATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTa                                                                                <  2:315203/140‑1 (MQ=18)
                                                      aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTaaa                                                                              >  1:275889/1‑138 (MQ=255)
                                                      aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                              <  1:171433/140‑1 (MQ=255)
                                                       ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                                                                             <  1:257941/140‑1 (MQ=255)
                                                       ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                                                                             >  1:45486/1‑140 (MQ=255)
                                                         ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCGTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCCTATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                           >  2:42945/1‑140 (MQ=255)
                                                           ccTTTTATTTTGATGTACGTCCCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTT‑GGGCAAATTCTTTCACCCAACGGCACACCGTTGAATGATGAACGTTTACACCACg                                                                         >  2:58959/1‑140 (MQ=14)
                                                           ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTCCACCACg                                                                         >  1:100845/1‑140 (MQ=255)
                                                           ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                         >  2:85485/1‑140 (MQ=255)
                                                           ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                         <  1:207605/140‑1 (MQ=255)
                                                            tttttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                        <  1:281667/139‑1 (MQ=18)
                                                             ttttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCACCGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                       >  1:95023/1‑140 (MQ=255)
                                                              tttATTTTGATGTACGTCTCATCAATACGCCAGTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTc                                                                      <  2:179968/140‑1 (MQ=255)
                                                              tttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCTAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGGATGATGAACGTTTACACCACGTTc                                                                      >  2:301718/1‑140 (MQ=11)
                                                               ttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTct                                                                     <  1:117853/140‑2 (MQ=255)
                                                                 tttctGATTTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctct                                                                   <  2:61668/136‑4 (MQ=11)
                                                                  ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTTCCtt                                                                  >  2:149085/1‑140 (MQ=19)
                                                                  ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                  <  1:133390/140‑1 (MQ=18)
                                                                    ttGATGTACGTCTCATCAATACGCCATTTGCAATAAGCTTTTTTCTGCTTTTTCTTCCAAATTTGATCCAAAATTGAGGGCTTTTTGAT‑AACCCAACTGCCGACCAAGTTATGATGACCGTTTACACCAGGTTCCCTTaa                                                                <  2:67657/140‑1 (MQ=9)
                                                                    ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                <  2:230953/140‑1 (MQ=11)
                                                                    gtGATATCCGTCTCCGCAATACGCCAGTTGTAATAAGCTTTTGTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑TGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                <  1:50778/139‑1 (MQ=11)
                                                                      gATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGATCCTAACGGTAGGTCGTTGAATGATGAGCGCTTTCTCCCCGTTCTCTTAata                                                              >  1:187857/1‑140 (MQ=11)
                                                                        tatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACGCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGTTCTCTTAATAtt                                                            >  2:27664/3‑140 (MQ=11)
                                                                        tatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGGTCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAtt                                                            >  1:128574/3‑140 (MQ=11)
                                                                           aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGCTCTCTTAATATTTCa                                                         >  1:230519/1‑140 (MQ=11)
                                                                            cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAGTATTTCAg                                                        >  2:137557/1‑140 (MQ=25)
                                                                            cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAg                                                        >  1:294509/1‑140 (MQ=255)
                                                                                tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTAAGatat                                                    >  2:101887/1‑140 (MQ=25)
                                                                                 catccATACCCCATTTGTAATCAGCTTTTTTATGCTTTTTCTTCCAATTTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatata                                                   <  1:15850/140‑1 (MQ=11)
                                                                                   tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                 <  2:254323/140‑1 (MQ=255)
                                                                                    caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                >  1:212669/1‑140 (MQ=255)
                                                                                    caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTCAACCCAACGGTCGATCGTTCAATGATGAACGTTTACACCACGTTCTCTTACTATTTCAGatatatac                                                >  1:144118/1‑138 (MQ=11)
                                                                                      aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGCTCGTTGAATGATGAACGTTTAAACTACGTTCTCTTAATATTTGAGATATATCagg                                              >  1:123760/1‑138 (MQ=11)
                                                                                       tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                             <  2:303561/140‑1 (MQ=255)
                                                                                       tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                             <  2:35503/140‑1 (MQ=255)
                                                                                       tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATCTTTCAGATATATCCCGa                                             >  2:132764/1‑140 (MQ=255)
                                                                                        tcGCTATTTGTAATAAGCTTTTTTATGCTTCTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACTCCTCGGTAGTCCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGAt                                            <  2:193569/139‑1 (MQ=11)
                                                                                        aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                            >  2:88394/1‑140 (MQ=255)
                                                                                         cGCCGTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATa                                           <  2:232443/140‑1 (MQ=16)
                                                                                          gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCGTTCCCTTAATATTTCCGATATTTCGCGATaa                                          >  1:296488/1‑140 (MQ=11)
                                                                                           ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGCTATACCGCCC‑ATACAGGTGCTAGAGTGCAACCGGAGATCGTTGAATGATTAACGTTTACATCACGTTCTCTTAATATTTCAGATATATCACGATagc                                         >  1:75382/1‑138 (MQ=2)
                                                                                           ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                         <  1:33903/140‑3 (MQ=11)
                                                                                           ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACAATagc                                         >  1:156411/1‑138 (MQ=11)
                                                                                           ccACTTGTAATAAGCTTTTTTATGCATTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGATCGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                         <  1:97096/140‑3 (MQ=11)
                                                                                              tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                      <  2:279583/140‑1 (MQ=18)
                                                                                              tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                      <  1:69881/140‑1 (MQ=21)
                                                                                               ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGCTAACTCaa                                     >  1:302352/1‑140 (MQ=21)
                                                                                                  taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTTAACCCAACGGTAAACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTAAGCTATATCATGATAACTCaacac                                  >  2:50910/1‑137 (MQ=11)
                                                                                                  taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCCCGATagcgcactgc                                  >  1:22707/1‑131 (MQ=11)
                                                                                                  taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATGTCACGATAACTCAATgc                                  >  1:262844/1‑139 (MQ=16)
                                                                                                   aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                 >  2:125489/1‑138 (MQ=11)
                                                                                                   aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACCATAGCTCAATgca                                 >  1:13704/1‑138 (MQ=11)
                                                                                                                 tGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagta                   <  2:295903/140‑1 (MQ=35)
                                                                                                                  gCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATAACTTAGAtagtag                  >  1:4352/1‑140 (MQ=25)
                                                                                                                                        |                                                                                                                                      
ACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGA  >  CP000730/36242‑36511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑