Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,413 C→T 59.5% V47I (GTC→ATC)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,4130CT59.5% 87.6 / 279.2 277V47I (GTC→ATC) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (64/48);  new base T (97/67);  total (161/116)
Fisher's exact test for biased strand distribution p-value = 8.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

Only 100 of 279 total aligned reads displayed.

ATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAAT  >  CP000730/36274‑36544
                                                                                                                                            |                                                                                                                                   
aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCCTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGAc                                                                                                                                     >  2:70952/1‑140 (MQ=25)
  ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCg                                                                                                                                   <  1:299041/140‑1 (MQ=40)
  ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAATCCAACGGTAGATCg                                                                                                                                   >  2:273975/1‑140 (MQ=11)
   gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAGAGAAAATTG‑GGGGATATTCTTTAACCCAACGGTAGAccct                                                                                                                                  >  1:59328/1‑138 (MQ=11)
     aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTATAAAAGTATCAAAATAAACTAG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTg                                                                                                                                >  1:312817/1‑140 (MQ=11)
      cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTGGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCAGATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                                               >  1:63854/1‑140 (MQ=255)
       gATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGTCTTGTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGaa                                                                                                                              <  1:283115/140‑1 (MQ=255)
         tataAATGGATACCTTTTCCTTTTATTTTGATATACTTCTCATCACTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCTAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                                            <  1:136972/140‑1 (MQ=11)
         tataAATAGCTCCATTTTCCTTTTCTTTTGATATACGTCTCATCAATACGCAATTTGTAAGAGGCTTTTTTATTCTTTTTCTTCCAAATTTGATATAAAATTT‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                                            >  1:61080/1‑140 (MQ=11)
         tataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCATGAACCCAACGGTAGACCGTTGAatg                                                                                                                            >  1:243220/1‑140 (MQ=25)
         tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                                            <  2:284098/140‑1 (MQ=255)
         tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                                            <  1:39508/140‑1 (MQ=255)
         tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                                            <  2:74653/140‑1 (MQ=255)
          ataAATAGCTCCATTTTTCTTTTATTTTTATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                                                           <  1:252870/140‑1 (MQ=11)
          ataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTT‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgt                                                                                                                           >  2:120825/1‑139 (MQ=11)
          ataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                                                           <  2:49912/140‑1 (MQ=255)
           taAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgat                                                                                                                          >  2:251503/1‑140 (MQ=255)
            aaatagcctctatttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgagg                                                                                                                         <  2:293546/128‑3 (MQ=11)
             aaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAAtgatga                                                                                                                        >  1:187829/1‑140 (MQ=18)
             aaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGGATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                                                        >  2:53692/1‑140 (MQ=11)
              aTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTCAatgagaaa                                                                                                                       >  2:66057/1‑136 (MQ=255)
              aTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                                                                                                                       <  2:96325/140‑1 (MQ=255)
              aTAGCGCCATGTTCCTTTTATTTTTATATACGTCTCATCAATACGTCATGTGGAAGAAGCTTTTGTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                                                                                                                       <  2:37586/140‑1 (MQ=11)
                  cTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                                                                                                                   <  2:264678/140‑1 (MQ=255)
                   tCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGttt                                                                                                                  <  1:13422/140‑1 (MQ=255)
                    ccATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTa                                                                                                                 <  2:315203/140‑1 (MQ=18)
                    ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                                                                                                                 >  1:128670/1‑140 (MQ=255)
                      aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                                               <  1:171433/140‑1 (MQ=255)
                      aTTTTCCTTTTACTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                                               <  1:123970/140‑1 (MQ=11)
                       ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTATACCGTTGAATGATGAACGTTTacac                                                                                                              >  1:278738/1‑140 (MQ=21)
                       ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                                              <  1:44723/140‑1 (MQ=21)
                        tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGATCGTTTAcacc                                                                                                             <  1:231502/140‑1 (MQ=16)
                        tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATCAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                                             <  2:189500/140‑1 (MQ=255)
                         ttCCTTTTATTTTGATGTACGTCTAATCAATACGGCATTTGTAATAAGCTTTTTTATGCTTTATATTCCAAATTTGATGTAGAATTA‑GGACATATTCTTGAACACACAGTTACACCGTTTACTAATCAACGGTTAccccc                                                                                                            >  2:42123/1‑139 (MQ=2)
                         ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                                                                                                            >  1:214365/1‑140 (MQ=255)
                          tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                                                           >  1:141186/1‑140 (MQ=255)
                           ccTTTTATTTTGATATCAATCGCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGATTACACCACg                                                                                                          >  1:317405/1‑140 (MQ=11)
                           ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTCCACCACg                                                                                                          >  1:100845/1‑140 (MQ=255)
                           ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                                                          >  2:85508/1‑140 (MQ=255)
                           ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                                                          <  1:207605/140‑1 (MQ=255)
                            cTTTTATTTTGATGTACGCCTCATCAAAACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                                                         <  1:147423/140‑1 (MQ=255)
                             ttttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt                                                                                                        <  1:186523/140‑1 (MQ=18)
                             ttttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGGATATTCTTGAACCCATCGGTAGATTGCTGAATGATGAACGTTTACACCACGtt                                                                                                        >  1:200505/1‑140 (MQ=11)
                             ttttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCACCGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                                                        >  1:95023/1‑140 (MQ=255)
                             ttttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                                                        >  1:65552/1‑140 (MQ=255)
                              tttATTTTGATGTACGTCTCATCAATACGCCAGTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTc                                                                                                       <  2:179968/140‑1 (MQ=255)
                              tttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCTAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGGATGATGAACGTTTACACCACGTTc                                                                                                       >  2:301718/1‑140 (MQ=11)
                                tATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                                                     <  1:197902/140‑1 (MQ=18)
                                tATTGTGATGTACGTCTCATCAATACACCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCCTATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                                                     >  1:218039/1‑140 (MQ=255)
                                 tttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGATTTTTCTTCCAAATTTGATATTAAATTG‑GTGCTTTGTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctct                                                                                                    >  2:235807/2‑140 (MQ=11)
                                  ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTTCCtt                                                                                                   >  2:149085/1‑140 (MQ=19)
                                  ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                   <  1:70228/140‑1 (MQ=21)
                                    ttGATGTACGTCTCATCAATACGCCATTTGCAATAAGCTTTTTTCTGCTTTTTCTTCCAAATTTGATCCAAAATTGAGGGCTTTTTGAT‑AACCCAACTGCCGACCAAGTTATGATGACCGTTTACACCAGGTTCCCTTaa                                                                                                 <  2:67657/140‑1 (MQ=9)
                                    ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGGATATTCTTGAACCCAACGGTAGATCGTTGACTGATGAACGTGTACCCCACGTTCTCTTaa                                                                                                 >  2:246255/1‑140 (MQ=11)
                                    ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                                                 <  1:15026/140‑1 (MQ=11)
                                    ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                                                 <  2:230953/140‑1 (MQ=11)
                                    gtGATATCCGTCTCCGCAATACGCCAGTTGTAATAAGCTTTTGTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑TGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                                                 <  1:50778/139‑1 (MQ=11)
                                     tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGATCCCTTAat                                                                                                >  2:10761/1‑140 (MQ=255)
                                      gATATACGTCTCATAAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTACAAATTTGATATAAAATTG‑GGGCATATTCTTGAAACCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTTTCTTAata                                                                                               >  2:5930/1‑140 (MQ=11)
                                       aTGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                                              >  2:158796/1‑140 (MQ=40)
                                        tatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGGTCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAtt                                                                                             >  1:128574/3‑140 (MQ=11)
                                         gTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAttt                                                                                            >  1:295702/1‑140 (MQ=255)
                                         atACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAAAGGTAGATAGTTGAATGTTGAACGTTTACACCACGTTCTCTTAATAttt                                                                                            <  1:303618/139‑1 (MQ=11)
                                          tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTc                                                                                           >  1:156506/1‑140 (MQ=21)
                                          tACGTCGCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTATTAATATTTc                                                                                           >  2:294951/1‑140 (MQ=11)
                                           aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCa                                                                                          >  1:115521/1‑140 (MQ=255)
                                            cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                                                         >  1:239764/1‑140 (MQ=255)
                                            cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAGTATTTCAg                                                                                         >  2:137557/1‑140 (MQ=25)
                                            cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAg                                                                                         >  1:294509/1‑140 (MQ=255)
                                             gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAAGTTTACACCCCGTTCTATTAATAttttgtg                                                                                        >  1:46663/1‑136 (MQ=11)
                                                tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatat                                                                                     <  2:61549/140‑1 (MQ=255)
                                                tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatat                                                                                     >  1:210082/1‑140 (MQ=21)
                                                  atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTATTATTTCAAatatat                                                                                   >  1:11973/1‑140 (MQ=11)
                                                   tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                                                  <  2:254323/140‑1 (MQ=255)
                                                       tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                                                              <  1:275101/140‑1 (MQ=21)
                                                       tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATCTTTCAGATATATCCCGa                                                                              >  2:132764/1‑140 (MQ=255)
                                                       tACGCCAATTGTAATAAGCTGTTCTATGCCTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                              <  2:22155/140‑1 (MQ=11)
                                                        aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                                             >  1:81450/1‑140 (MQ=255)
                                                          gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                           <  1:251491/140‑2 (MQ=255)
                                                          gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                           >  1:307523/1‑139 (MQ=255)
                                                          gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                           >  2:112231/1‑140 (MQ=25)
                                                           ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                                          >  1:257984/1‑138 (MQ=255)
                                                           ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                                          <  1:33903/140‑3 (MQ=11)
                                                           ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTACTATTTCAGATATATCACGATAAc                                                                          >  2:90814/1‑140 (MQ=255)
                                                           ccACTTGTAATAAGCTTTTTTATGCATTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGATCGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                                          <  1:97096/140‑3 (MQ=11)
                                                                 gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCGACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                    >  2:35310/1‑140 (MQ=11)
                                                                 gTAATAAGCTTTTTTATGACTATTATACCAAATACGAGGTAAAATTT‑GGGCATATTGTTGACCCCAACAGTAGGGCTGTTAAGGGGCGGCCTTGACACCACTTTCCCGTTATATTTCAGATATATCACGATAACTCAATg                                                                    <  2:27888/140‑1 (MQ=2)
                                                                  taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGTTATATCACCATAGCTCAATgc                                                                   >  2:41429/1‑139 (MQ=11)
                                                                  taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATGTCACGATAACTCAATgc                                                                   >  1:262844/1‑139 (MQ=16)
                                                                  taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCACTgc                                                                   >  1:229535/1‑139 (MQ=16)
                                                                   aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCGTATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                                                  <  2:218039/140‑3 (MQ=12)
                                                                   aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                  <  2:279768/140‑3 (MQ=11)
                                                                   aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTGTACTCCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                  <  2:176336/140‑3 (MQ=11)
                                                                   aataaGCTTTTTTATGCTTTTTCTTCCAAATGTGATATAAAATTG‑GAGCATAGTCTTCACAGCAACGATTGACCGTTGAATGATGAACGTTTACACCACGATCCCTTAATATTTCAAATATATCACAATAACTCAAtaca                                                                  >  2:286840/1‑137 (MQ=11)
                                                                                aTGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAACGTATATCTTAGAtagt                                                     <  1:158796/140‑1 (MQ=25)
                                                                                 tGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagta                                                    <  2:295903/140‑1 (MQ=35)
                                                                                       ttcttcCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAAc                                              >  2:12640/1‑140 (MQ=35)
                                                                                                                  ggCATATTCTTAAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaa                    <  1:91274/140‑1 (MQ=18)
                                                                                                                                 ccAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGa     <  2:275889/140‑1 (MQ=33)
                                                                                                                                    aCGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAATTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAAt  >  2:205027/1‑140 (MQ=18)
                                                                                                                                            |                                                                                                                                   
ATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTG‑GGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAAT  >  CP000730/36274‑36544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑