Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,185 G→A 15.1% intergenic (+243/‑891) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,1850GA15.1% 98.7 / 5.7 50intergenic (+243/‑891)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (15/26);  new base A (2/6);  total (17/33)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGA  >  CP000730/76053‑76324
                                                                                                                                    |                                                                                                                                           
aCTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAtt                                                                                                                                      >  1:257309/1‑140 (MQ=255)
  taattatgcagcACCTGTAACAACTAGAACAGCTAACCAAATTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAAGTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAtttt                                                                                                                                    <  1:30539/134‑1 (MQ=255)
      atTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGTTTCTGTTGCAAAGTTGAATCTATAATATAATTTTAACa                                                                                                                                >  1:104688/1‑140 (MQ=255)
                 cgcaccACTTAGAACACCTAACCAAAGCGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAaaaaaaccgagcct                                                                                                                     <  2:276865/135‑9 (MQ=38)
                   tATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtcttc                                                                                                                   >  1:175281/1‑140 (MQ=255)
                         ttAGAACAGCTAACCAAAGTTTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAATAGGAGTCTTCTGTATg                                                                                                             <  1:159107/140‑1 (MQ=255)
                         ttAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATg                                                                                                             <  1:292933/140‑1 (MQ=255)
                                    aaCCAAATGTTTATTAATCCAAGGAATACTAACACAAATGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAg                                                                                                  <  2:37187/140‑1 (MQ=25)
                                      ccAAAGTGTTAGTAATCAAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGat                                                                                                <  1:239441/140‑1 (MQ=255)
                                         aaGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGAtata                                                                                             >  1:272500/1‑140 (MQ=255)
                                           gtgtTAGTAATTCAGGGAATACTAACACAAAGGCAATTAATTTCATAATAAGAGCAAAATATAGGTTCTGTTGCAAAGTTGAATGTATAGTATAATTAAAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATaaa                                                                                           <  2:239156/140‑1 (MQ=21)
                                           gtgtTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTAAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATaaa                                                                                           <  2:81817/140‑1 (MQ=255)
                                                agtattccctggattACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAtt                                                                                      >  2:120218/15‑140 (MQ=21)
                                                       cAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTTAATCTATAGTATAATTTTAACAAAAAGGAGACTTCTGTATGAACTATTTACGTAATAAAAAATATAAAAAg                                                                               >  1:128473/1‑140 (MQ=25)
                                                        aGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAgg                                                                              <  1:133304/140‑1 (MQ=255)
                                                         gggAATACTAACACAAAGGCAATTAAATTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGa                                                                             <  2:135273/140‑1 (MQ=255)
                                                          ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGAt                                                                            >  2:229671/1‑140 (MQ=255)
                                                                   acacAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACt                                                                   >  1:15457/1‑140 (MQ=255)
                                                                     acaAAGGGAATTAATTTAATAATTATAGAAACATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAAAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGt                                                                 <  2:318141/140‑1 (MQ=17)
                                                                         aGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                             <  1:85714/140‑1 (MQ=255)
                                                                             aattaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTg                                                         <  2:290795/140‑1 (MQ=255)
                                                                              attaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTgg                                                        >  1:8750/1‑140 (MQ=255)
                                                                                      aataatTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATc                                                >  1:151854/1‑140 (MQ=255)
                                                                                                  atataggaGGTTCTGTTGCAAAGTTGAATTTATAATATAAGTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACGAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                    <  2:11328/133‑1 (MQ=11)
                                                                                                  agttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTATCCGTTGGATACTATCTCAGCTATGCAt                                    >  2:186580/8‑140 (MQ=11)
                                                                                                    ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                                  <  1:70295/135‑1 (MQ=255)
                                                                                                    aaatgaGGTTCTGTTGAAAAGTTGAATTTATAGTATAACTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                                  <  1:26498/135‑1 (MQ=11)
                                                                                                    aaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                                  <  2:36335/140‑1 (MQ=40)
                                                                                                    aaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                                  <  2:36338/140‑1 (MQ=40)
                                                                                                     aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                                 <  1:9737/136‑1 (MQ=9)
                                                                                                      tataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTGACAAAACGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                                >  2:207613/1‑140 (MQ=255)
                                                                                                      tataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                                <  2:35206/140‑1 (MQ=40)
                                                                                                      atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                                <  1:202561/137‑1 (MQ=9)
                                                                                                      aggaGGTTCTGTTGCAAAGTTTAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                                <  2:253436/137‑1 (MQ=255)
                                                                                                        actgTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                              <  2:112362/137‑3 (MQ=2)
                                                                                                         aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                             >  1:111098/1‑140 (MQ=11)
                                                                                                             tCTTTTGCAAAGTTGAATATATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCTGATATAAATAAGTTAACACGGATGTTATCACTGTAGCAGTTGGCTACTATCTAAGATATGCATTGAGCTATCGt                         <  2:82011/140‑1 (MQ=11)
                                                                                                               tGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGa                       <  2:8750/140‑1 (MQ=35)
                                                                                                                 ttacAAAATTAAATATAAAGAAAAATATAAACAAAAAAGAGTCTACTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGata                     <  1:23978/137‑1 (MQ=11)
                                                                                                                     aaaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc                 <  1:229671/140‑1 (MQ=37)
                                                                                                                       aGTTGAATCTATAATATAAATATAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTg               <  2:222084/140‑1 (MQ=255)
                                                                                                                        gTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa              <  1:120218/140‑1 (MQ=35)
                                                                                                                         ttGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaa             >  1:13899/1‑140 (MQ=35)
                                                                                                                             atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAatat         <  2:255704/137‑1 (MQ=2)
                                                                                                                             atttataGTATAATTTTAACAAAAAGGAGTATTCTGAATGAGCTATTTCCGCTATAAAAAATTAAACAAGGATAAAATCAATGTAATTGTTGACAACTATCCTAGATAAGCTTGAAGTGATCGTGATATACCTGAAatat         >  2:75540/4‑140 (MQ=0)
                                                                                                                              tttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt        >  1:302974/3‑140 (MQ=2)
                                                                                                                              tttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAAATATGCATTGAGCTATCGTGATATATCTGAAATAtt        >  2:83956/3‑140 (MQ=2)
                                                                                                                               ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTa       >  1:88394/2‑140 (MQ=9)
                                                                                                                                tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGTATTGAGCTATCGTGATATATCTGAAATATTaa      <  1:300607/140‑1 (MQ=255)
                                                                                                                                tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa      <  2:125644/140‑1 (MQ=12)
                                                                                                                                tataatataaTTTTAACAAAAAGGAGTACTTTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTAACACTGTTGCCGTTGGCTACTATTTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa      <  1:34367/140‑1 (MQ=11)
                                                                                                                                tataGTACAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTaa      <  2:175281/140‑1 (MQ=18)
                                                                                                                                tataCTATAATTGTAACAAAAAGGGTTCGTCTGTGTGAACTATGTCCGATATAAACAATTTAACAAGGATGTGATCTCTGGAGCGGCTGGCTACTATCTAAGGTATGCATTGAGTTATCGTGATACATCTGAAATATTaa      <  2:48119/140‑1 (MQ=11)
                                                                                                                                 ataatataatTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAg     <  1:47831/140‑1 (MQ=18)
                                                                                                                                 ataatataatTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAg     <  1:218961/140‑1 (MQ=12)
                                                                                                                                  taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAgg    >  1:132606/1‑140 (MQ=18)
                                                                                                                                  taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAgg    >  1:266688/1‑140 (MQ=33)
                                                                                                                                   aGTATAATTTTAACAAAAAGTAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTTAGTTATCGTGATATATCTGAAATATTAAggg   <  1:289227/140‑1 (MQ=16)
                                                                                                                                   aGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTTGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAggg   <  1:233645/140‑1 (MQ=18)
                                                                                                                                    gTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGa  >  1:102698/1‑140 (MQ=17)
                                                                                                                                    gTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGa  <  1:57867/140‑1 (MQ=33)
                                                                                                                                    |                                                                                                                                           
ACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGA  >  CP000730/76053‑76324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: