Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,295 T→C 53.3% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2950TC53.3% 48.2 / inf 293intergenic (+353/‑781)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base T (66/71);  new base C (84/72);  total (150/143)
Fisher's exact test for biased strand distribution p-value = 3.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01

Only 100 of 293 total aligned reads displayed.

AGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGC  >  CP000730/76158‑76433
                                                                                                                                         |                                                                                                                                           
aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                                                                                                                                           >  1:111098/1‑140 (MQ=11)
            aaaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc                                                                                                                               <  1:229671/140‑1 (MQ=37)
                ttGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaa                                                                                                                           >  1:13899/1‑140 (MQ=35)
                    atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAatat                                                                                                                       <  2:255704/137‑1 (MQ=2)
                     tttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt                                                                                                                      >  1:302974/3‑140 (MQ=2)
                     tttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAAATATGCATTGAGCTATCGTGATATATCTGAAATAtt                                                                                                                      >  2:83956/3‑140 (MQ=2)
                       tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGTATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                                                    <  1:300607/140‑1 (MQ=255)
                       tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                                                    <  2:125644/140‑1 (MQ=12)
                       tataatataaTTTTAACAAAAAGGAGTACTTTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTAACACTGTTGCCGTTGGCTACTATTTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                                                    <  1:34367/140‑1 (MQ=11)
                         taatataATTTTAACAAAAAGGAGTCTTCTGTGTGAACTATTTCAGATATAATCAATTTAACAAGGATGTTATCTCTGTAGCCGTTGGCTACTATCTCAGATATGCATTGAGCTATCGTGATATATCTGAAATGTTAAga                                                                                                                  >  2:138543/4‑139 (MQ=11)
                         taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAgg                                                                                                                  >  1:132606/1‑140 (MQ=18)
                          aGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTTGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAggg                                                                                                                 <  1:233645/140‑1 (MQ=18)
                           gTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGa                                                                                                                >  1:102698/1‑140 (MQ=17)
                                aTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtg                                                                                                           >  1:235807/1‑140 (MQ=16)
                                 ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtgg                                                                                                          <  2:21882/140‑1 (MQ=16)
                                     aaCAAAAGGGAGGCTTCTGGATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTa                                                                                                      >  2:187857/1‑140 (MQ=11)
                                     aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtggtgca                                                                                                      >  2:220550/1‑138 (MQ=12)
                                      aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACACGGATGTTATCACTGTAGCTGTTGGCTACTATATAAGAGATGCATTGAGTTATCATGATATATTTGAAATATTAAGGGAACGTGGTGTaa                                                                                                     >  1:8938/1‑140 (MQ=11)
                                      aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTaa                                                                                                     >  2:248644/1‑140 (MQ=33)
                                       cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaaa                                                                                                    >  2:212669/1‑140 (MQ=12)
                                           aaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGtt                                                                                                >  2:280799/1‑140 (MQ=12)
                                           aaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAAAATTAAGCGAACTTGGTGTAAACGtt                                                                                                >  2:88773/1‑140 (MQ=255)
                                            aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGtcc                                                                                               >  2:117590/1‑138 (MQ=16)
                                            aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtc                                                                                               <  1:310917/140‑1 (MQ=16)
                                               aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatc                                                                                            <  1:261563/140‑1 (MQ=18)
                                               aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatc                                                                                            <  1:222426/140‑1 (MQ=16)
                                                gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatca                                                                                           <  2:32620/140‑1 (MQ=17)
                                                  cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTACCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAGACGTTCATCAtt                                                                                         >  1:294951/1‑140 (MQ=255)
                                                   ttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGCACGAGGTGTAAAAGTTCATCATTc                                                                                        >  2:287565/1‑140 (MQ=11)
                                                        tATGGACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGCGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACgg                                                                                   <  2:52480/140‑1 (MQ=12)
                                                        tATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACgg                                                                                   <  2:15457/140‑1 (MQ=32)
                                                          tGAACTATTTCAGATATCAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATc                                                                                 <  2:58418/140‑1 (MQ=11)
                                                           gAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTCATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGtag                                                                                <  1:212560/140‑3 (MQ=12)
                                                            aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGCGGGGTAAACGTTCATCATTCAACGATCTa                                                                               >  1:42501/1‑140 (MQ=11)
                                                              cTATTTCAGATATTAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                             <  2:17248/140‑1 (MQ=255)
                                                                 tttCAGTTATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                                                          <  2:168418/140‑1 (MQ=18)
                                                                 tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAATAGAACGTAGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                          <  2:225905/140‑1 (MQ=11)
                                                                 tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                          <  1:9536/140‑1 (MQ=255)
                                                                  ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTg                                                                         <  2:271093/140‑1 (MQ=255)
                                                                    cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTCCCGTTggg                                                                       >  1:128705/1‑140 (MQ=17)
                                                                    cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATCTCTGAAATATTAAGAGAACGTGGTGTAAACGTTCCTCCTTCAACGATCCACCGTTggg                                                                       >  2:302240/1‑140 (MQ=11)
                                                                      gttataCACAATTTAGCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGtt                                                                     <  2:111663/138‑1 (MQ=255)
                                                                      cctataAACAATTTAACAAGGTTGTTATCAATGTAGTTGTTGGCTACTATCTAGGTTATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                     <  1:4369/138‑1 (MQ=11)
                                                                       atatAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTc                                                                    >  1:119896/1‑140 (MQ=17)
                                                                        tataAACAATTTAACAGGGATGTTATCACTGTAGCTGTGGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCa                                                                   >  1:178844/1‑140 (MQ=12)
                                                                        tataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTTAGCTATCGTGATATATCTGAAATATTAAAAGAACGAGGTGTAAGAGTTCATGATTCAACGACCTACAATTGGGTTCa                                                                   >  2:311065/1‑140 (MQ=11)
                                                                        tataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCa                                                                   <  1:275790/140‑1 (MQ=255)
                                                                          taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTGATCATTCAACGGTCTACCGTTGGGTTCAAg                                                                 >  2:96393/1‑140 (MQ=18)
                                                                           aaaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTAAAGa                                                                >  2:43409/1‑140 (MQ=17)
                                                                            aaCAATTTACCAAGCATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGaa                                                               <  2:223934/140‑1 (MQ=11)
                                                                             aCAATTTAACAAGGATTTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAat                                                              <  2:143026/140‑1 (MQ=17)
                                                                               aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                            >  2:200319/1‑140 (MQ=255)
                                                                                aTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                                                           >  2:312817/1‑140 (MQ=12)
                                                                                 tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCAAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGc                                                          >  2:6534/1‑140 (MQ=255)
                                                                                  ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                                                         <  1:115204/140‑1 (MQ=255)
                                                                                   tAACAAGGATGTTATTACTGTAGCTGTTGGCTACTATCTAAGATATGTATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGccc                                                        <  1:189929/140‑1 (MQ=12)
                                                                                    aaCACGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcccc                                                       <  2:113546/140‑1 (MQ=255)
                                                                                    aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcccc                                                       >  1:291066/1‑140 (MQ=33)
                                                                                    aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcccc                                                       >  1:147898/1‑140 (MQ=255)
                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTACGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                                                      >  2:264750/1‑140 (MQ=11)
                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                                                      >  1:266779/1‑140 (MQ=255)
                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                                                      >  1:258688/1‑140 (MQ=16)
                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                                                      >  2:150616/1‑140 (MQ=255)
                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCACCa                                                      >  2:157466/1‑140 (MQ=255)
                                                                                     aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGCTTCAAGAATATGCCCCa                                                      >  1:173729/1‑140 (MQ=255)
                                                                                          gATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtttt                                                 >  1:59969/1‑140 (MQ=255)
                                                                                            tGTCATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGGGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                               <  2:47737/140‑1 (MQ=11)
                                                                                              ttATCACTGTAGCTGTTGGCTACTATATAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                             >  1:239177/1‑140 (MQ=25)
                                                                                              ttATCACCGTAGCTGTTGGCTAATACTTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAACCGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                             <  1:75910/140‑1 (MQ=11)
                                                                                                attaCTGTAGCTGTTGGCTATTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                           <  1:107409/137‑1 (MQ=255)
                                                                                                agcACTGTAGCCGTTGGCTAATATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                           <  2:197292/138‑1 (MQ=11)
                                                                                                aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                           <  2:266916/140‑1 (MQ=255)
                                                                                                aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                           >  1:156632/1‑140 (MQ=255)
                                                                                                 tCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa                                          >  1:204869/1‑140 (MQ=255)
                                                                                                   aCTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGTTGTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAt                                        >  1:216914/1‑140 (MQ=255)
                                                                                                   aCTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAt                                        >  2:131228/1‑140 (MQ=255)
                                                                                                      gTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg                                     >  1:269639/1‑140 (MQ=25)
                                                                                                       tAGCTGTTGGCTACGTTCTAAGATATGCATTGATTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCTTCATTTAACGGTCTACCGTTGGGTTCAAGAATATGCCTCAATTTTATATCAAATTTgg                                    <  1:194654/140‑1 (MQ=11)
                                                                                                        aGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                   <  2:232992/140‑1 (MQ=255)
                                                                                                         gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAGCGTAGGGTTTAAGAATATGCCCCAATTTTATATCAAGTTTGgag                                  >  1:59392/1‑139 (MQ=11)
                                                                                                          ctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                 <  1:28744/138‑1 (MQ=37)
                                                                                                           tgTTGGCTACTATCTAAGGTGTGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGTACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga                                <  1:184272/139‑1 (MQ=255)
                                                                                                              tGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaa                             >  2:54145/1‑140 (MQ=255)
                                                                                                               ggCTACTATCTAAGATATGCATTGAGTTAGCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaaa                            <  1:50535/140‑1 (MQ=25)
                                                                                                                  tactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                         >  1:244073/1‑140 (MQ=255)
                                                                                                                  tactaTCTAAGATATGCATGGAGTTAGCGTGATATATCTGAAATATTAAGGGAACGTGGTTTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGCTTATGCCCTAATTTTATATCAAATTTGGAAGAAAAAGCa                         <  2:76384/140‑1 (MQ=11)
                                                                                                                  tactaTCTAAGATAGGCATGGAGTTAGCTTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                         <  2:24032/140‑1 (MQ=255)
                                                                                                                   actaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                        <  1:159939/140‑1 (MQ=35)
                                                                                                                   actaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAAAATATACCCCAATTTTAAACCAAATTTGGAAGAACAAGCAt                        >  1:16978/1‑140 (MQ=11)
                                                                                                                     taTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                      <  2:109447/140‑1 (MQ=34)
                                                                                                                       tCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa                   <  1:252883/140‑1 (MQ=255)
                                                                                                                        ttAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaa                  <  2:294162/139‑1 (MQ=255)
                                                                                                                        cTAAGATATGCATCGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaa                  <  2:274566/140‑1 (MQ=255)
                                                                                                                           atatatGCATTTAGCTCGCTTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagc               <  2:71386/138‑4 (MQ=11)
                                                                                                                           aGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAGGCATaaaaaagc               >  1:70952/1‑137 (MQ=18)
                                                                                                                           aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagc               >  1:273592/1‑137 (MQ=11)
                                                                                                                            gagtggCAGTGGGTTAGCGTGATAGAGCTGAAATAGTAAGGGAAGGTGGTGTAAGCGTTCATCATTCAACGGTCTAGCGTTTGGTTCAAGAATATGCCCCAATTTTATAGCAAATTTGGAAGAAAGAGCATaaaaaagct              <  2:61033/135‑5 (MQ=1)
                                                                                                                            gATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAggct              >  2:127804/1‑137 (MQ=11)
                                                                                                                             atatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACTTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagctt             <  1:122010/140‑6 (MQ=11)
                                                                                                                              tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAGAAGCtta             >  1:36643/1‑138 (MQ=9)
                                                                                                                                         |                                                                                                                                           
AGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGC  >  CP000730/76158‑76433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: