Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 367,644 G→T 100% intergenic (‑96/‑20) nanA ← / → USA300HOU_0336 N‑acetylneuraminate lyase/possible transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730367,6440GT100.0% 86.6 / NA 30intergenic (‑96/‑20)nanA/USA300HOU_0336N‑acetylneuraminate lyase/possible transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/14);  total (16/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTAGTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAAT  >  CP000730/367521‑367774
                                                                                                                           |                                                                                                                                  
cATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTAtaa                                                                                                                    >  2:313479/1‑140 (MQ=255)
           tttAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAAt                                                                                                         <  2:140048/140‑1 (MQ=255)
              aaaTCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGtt                                                                                                      <  1:184013/140‑1 (MQ=255)
               aaTCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTTTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATGTTTATGTATAATTAAAGGAAATGttt                                                                                                     >  1:253831/1‑140 (MQ=255)
                   tttGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCa                                                                                                 >  2:66166/1‑140 (MQ=255)
                       ccacgtctGAAGTGTCTCAATATATCATTTGATAATATAGTTTACAGATATAATTGTAAACGCTATCATAAAATGTAACAACATCTGTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGATTTTCAAAAt                                                                                             <  2:298220/133‑1 (MQ=255)
                           ttgggAAGTCACTCCAAAGATCTTTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTGTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAAt                                                                                         <  1:291785/136‑1 (MQ=255)
                             aTGAACTCGCGCCAAAAATCATTTGATAACATTCGCCACAGCTATAATGGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGCTTTTCAAAATCAATAg                                                                                       <  2:145184/140‑1 (MQ=255)
                                aaGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGaaa                                                                                    >  1:183533/1‑140 (MQ=255)
                                   tCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAACTgg                                                                                 >  2:110971/1‑140 (MQ=255)
                                        ccAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagtga                                                                            >  2:243704/1‑140 (MQ=255)
                                          aaaaaTCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagtgagt                                                                          <  1:313479/140‑1 (MQ=255)
                                             aaTCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAg                                                                       <  2:20281/140‑1 (MQ=255)
                                             aaTCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAg                                                                       >  2:29028/1‑140 (MQ=255)
                                                cATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAATATTTTATTCATATTGATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGATCAAGTAAGatg                                                                    >  2:31083/1‑140 (MQ=255)
                                                               tttACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATATTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGGGAGTAAGGGGTAGTACATCTCAATc                                                     >  1:117475/1‑140 (MQ=255)
                                                                 tACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTACGGTGTATTACATCGCAATCGa                                                   >  2:293922/1‑140 (MQ=255)
                                                                 tACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGa                                                   <  2:32687/140‑1 (MQ=255)
                                                                       tataATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATAttag                                             >  1:293471/1‑138 (MQ=255)
                                                                       tataATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTgg                                             >  2:91561/1‑140 (MQ=255)
                                                                             tGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGGGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCAc                                       >  1:248918/1‑140 (MQ=255)
                                                                                   cGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAAt                                 <  2:9951/140‑1 (MQ=255)
                                                                                    gCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAAtt                                >  2:282874/1‑140 (MQ=255)
                                                                                     atATCAAAAAATGTAACAATATTTTTTTTAAAATTTTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTa                               <  2:117475/139‑1 (MQ=255)
                                                                                       aTCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTaaa                             >  1:189646/1‑140 (MQ=255)
                                                                                               aTGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCCCTCAAATTAAATCGGCAGt                     <  2:245022/140‑1 (MQ=255)
                                                                                                        tatCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATaa            <  1:299124/140‑1 (MQ=255)
                                                                                                         atCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAg           <  2:2229/140‑1 (MQ=255)
                                                                                                                 gAAAATGGTATTCATTTTTATGTTTAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGaa   <  1:29033/140‑1 (MQ=255)
                                                                                                                  aaaaTTGTATTCATCTTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGaca  >  2:70293/1‑138 (MQ=255)
                                                                                                                           |                                                                                                                                  
CATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTAGTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAAT  >  CP000730/367521‑367774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑