Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 707,879 A→T 100% L11I (TTA→ATA)  USA300HOU_0653 ← ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730707,8790AT100.0% 85.0 / ‑6.5 30L11I (TTA→ATA) USA300HOU_0653ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (7/22);  total (8/22)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAAT  >  CP000730/707749‑708013
                                                                                                                                  |                                                                                                                                      
gCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGAtt                                                                                                                               <  1:28553/140‑1 (MQ=255)
  aTTAAATTCACCAATTAAAGACGTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTa                                                                                                                             <  2:176567/140‑1 (MQ=255)
                ttAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCt                                                                                                               <  1:148848/140‑1 (MQ=255)
                 tAAAGACTTGATAAGGGAAGATTTACCAGCTCCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTCAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTa                                                                                                              <  1:249778/140‑1 (MQ=255)
                 tAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTa                                                                                                              <  2:278913/140‑1 (MQ=255)
                  aaaGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTaa                                                                                                             <  1:125192/140‑1 (MQ=255)
                          gATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACaatcctt                                                                                                     >  2:232829/1‑135 (MQ=255)
                             aaGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTtat                                                                                                  <  1:280536/140‑1 (MQ=255)
                             aaGGGAACATTTACAAGCACCATGCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGtttct                                                                                                  >  2:268046/1‑138 (MQ=255)
                               gggAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTTGTAAGGATAAGGAAAAGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATGTTTTGTTTCGATCAAGCGTTTATAc                                                                                                >  2:236856/1‑140 (MQ=255)
                                  aaGATTTACCAGCCCCATTTGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATCAGGAAATGTTTTTAAGTACATGCTTACTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAACCGTTTATAcctc                                                                                             <  1:318297/140‑1 (MQ=255)
                                                 cATTCGGGCCCATGATACCAATTATTTCGACGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTa                                                                              <  2:255074/140‑1 (MQ=255)
                                                  aTTCGGGCCCATGATACCAATTATTTCGCCGCGTACGGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTAGACCTCCTAATTAAAAGTTTAg                                                                             >  1:221182/1‑140 (MQ=255)
                                                   ttCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTCATTAAAAGTTTAgg                                                                            <  2:219114/140‑1 (MQ=255)
                                                    tCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGc                                                                           <  1:163377/140‑1 (MQ=255)
                                                       ggCCCATCATACCAATTTTTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTaa                                                                        <  2:312543/140‑1 (MQ=255)
                                                                              acgcgTACTGGCATCGATAAGGAAATGTTTTTAAGGACAAGCTTATTACCTCTAACTCGAGTTAAATGTTTTGCTTCTAACAAACACACACTGTGCCTAATGCAAAGTTTAGGCTCACCTAATTAATTGTATAATAAAcg                                                 >  2:94081/2‑139 (MQ=255)
                                                                                     cttgTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAata                                          <  1:71964/137‑1 (MQ=255)
                                                                                           tCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATc                                    <  2:302973/140‑1 (MQ=255)
                                                                                                   gAAGTGTTTGTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTGTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                            <  1:207931/140‑1 (MQ=255)
                                                                                                   gAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                            <  1:297574/140‑1 (MQ=255)
                                                                                                   gAAATGTGTTTAAGTACATTCTTACTACCTATAAACAGATTTAACTCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                            <  1:313067/140‑1 (MQ=255)
                                                                                                     aaTGTTTTTCAGTACACGCTTATCACCTATAAGCAGATTTAAACCGTTTGTTTCTAACAAACGTTCATACCTCCTAATTAAAAGTTTAGGCCAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                          <  1:23066/140‑1 (MQ=255)
                                                                                                     aaTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                          <  2:57920/140‑1 (MQ=255)
                                                                                                     aaTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                          <  2:55832/140‑1 (MQ=255)
                                                                                                      aTGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCCAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAATaa                         >  1:214924/1‑140 (MQ=255)
                                                                                                           tttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCg                    >  2:72926/1‑140 (MQ=255)
                                                                                                           tttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCGAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCg                    <  1:60975/140‑1 (MQ=255)
                                                                                                            ttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGt                   >  1:249653/1‑140 (MQ=255)
                                                                                                                             aCCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAat  <  2:318905/140‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                      
GCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAAT  >  CP000730/707749‑708013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑