Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,481,355 G→T 100% A6890E (GCA→GAA)  ebh ← extracellular matrix binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,481,3550GT96.4% 62.4 / ‑6.1 25A6890E (GCA→GAA) ebhextracellular matrix binding protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (10/13);  total (11/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGC  >  CP000730/1481222‑1481491
                                                                                                                                     |                                                                                                                                        
cGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGt                                                                                                                                    >  2:139565/1‑140 (MQ=255)
             tCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGt                                                                                                                       <  1:226970/140‑1 (MQ=255)
             tCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGt                                                                                                                       <  2:228955/140‑1 (MQ=255)
              ccTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGtt                                                                                                                      >  1:206352/1‑140 (MQ=255)
                      gTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCt                                                                                                              <  1:279510/140‑1 (MQ=255)
                              ggCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCAt                                                                                                      <  1:145315/140‑1 (MQ=255)
                                   gCGGTACTGCAGCTACAGTGGGCGCTTGATTGGTGTTTCCAGTAACAGCTCCTTTTTGTGCATTGTTTAAGTTATCTAGCGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAAc                                                                                                 <  2:1161/140‑1 (MQ=255)
                                         cTTCAGCGACAGTGTGCGCTTGATTGATGTTTCCAGTAACATCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGt                                                                                           <  2:125689/140‑1 (MQ=255)
                                          ttCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAAAGTTGATTGTTTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGtt                                                                                          >  1:152643/1‑140 (MQ=255)
                                              gCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTGTATCACCATTCAACGCTTGTTTCGt                                                                                      >  2:5873/1‑140 (MQ=255)
                                                     tgagcgcTTGATTGATCTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgttgtt                                                                               <  2:213986/137‑1 (MQ=255)
                                                                   atGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGCAAGtgc                                                                 >  1:40291/1‑140 (MQ=255)
                                                                       tgtcAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTgctgct                                                             <  2:278510/137‑1 (MQ=255)
                                                                        tCCAGTAACAGCACCTTTTTGTGCATCGTTTAAGTTATCTAACGGAGATAATGTTTGGTTTTGATTGTTTTTAGCATCGGTTACTATCCTATCAACATTCAAGGCTTGTGTCGTAGTTGTTATAGTTGCAAGtgccgtgt                                                            >  2:64270/1‑135 (MQ=255)
                                                                                 aGCACATTTTTGTGCACTGTTTAAGTTATCGAACGAAGATAATGTTTGGTCAGCATTGTTTTTAGCATCTGTTACTTTTCTATCAACTCTTAACGCCAGTTTCGTAGTTGTTACAATTGAAAGTCCTCCTTCCACTtgtg                                                   >  2:135343/1‑140 (MQ=255)
                                                                                                 ttGTTTTAGTTTTCTATCTTTGTTATTTTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCTCCATTTAACGCTTGTTTCGTAGTTGTTACTGTTGATAGTGCTGCTTCAACTTGTGTTTGGTTCGCATTTGt                                   <  2:39054/140‑1 (MQ=255)
                                                                                                    tttAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGACAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTAcc                                <  1:211287/140‑1 (MQ=255)
                                                                                                      tAAGTTATCTTACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCAt                              <  1:193477/140‑1 (MQ=255)
                                                                                                                  cGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAAGTTGTGATTGGTTCGCATTTGTCCCATTTGCTTGATtaa                  <  1:178569/140‑1 (MQ=255)
                                                                                                                  cGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATtaa                  >  2:89714/1‑140 (MQ=255)
                                                                                                                  cGTAGATAATGTTTCGTTTTCATTGTTTTTAGCATCGGTTACATTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCAGCTTCAACTTGTGATTGGTTCGCATTTGTACCACTTGCTTGCTtaa                  <  1:225543/140‑1 (MQ=255)
                                                                                                                      gATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGATTCGCATTTGTACCATTTGCTTGATTaataat              >  2:178569/1‑140 (MQ=255)
                                                                                                                        tAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAatat            >  1:100240/1‑140 (MQ=255)
                                                                                                                         aaTGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATAtt           >  1:208016/1‑140 (MQ=255)
                                                                                                                          aTGTTTGCTTCTCATTGTTTTTATCATCTGTTACTTTTCTAGCACCATTCAGCGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCAGTTGTACCATTTGCTTGATTAATAATAttt          <  2:103247/140‑1 (MQ=255)
                                                                                                                              ttggttttCATTGTTTTTAGCATCTGTTACTTTTATATTACCATTCCAAGATTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTcag      <  1:24799/140‑1 (MQ=255)
                                                                                                                                  ttttCATTGTTTTTAGCATCAGTTACTTTTTTATCTCTATTCTACGCTTGTTTCCTTGTCTGTCTAGTTCATAGAGCTCCTGCAATGTGTGATTGTTTCGCCTTTGTACAAGTTTCTTGATTAATAATATTTTcagcagc  <  2:80360/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
CGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGC  >  CP000730/1481222‑1481491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: