Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,797,772 G→C 100% R7G (CGC→GGC)  USA300HOU_1679 ← sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,797,7720GC96.0% 53.2 / ‑5.8 18R7G (CGC→GGC) USA300HOU_1679sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (11/6);  minor base A (1/0);  total (12/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATATATGATCTTGCATCTGTTGCTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCGGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACA  >  CP000730/1797639‑1797898
                                                                                                                                     |                                                                                                                              
atatatGATCTTGTATCTGTTGCTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCgtggtg                                                                                                                          <  1:107041/140‑1 (MQ=255)
          ttGCATCTGTTGCTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTGACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGGGAGACTtcatc                                                                                                                >  1:108607/1‑140 (MQ=255)
                cTGTTGCTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCCATAACATTAAGCCGTGGTGAAACTTCATCATTGtt                                                                                                          >  2:22678/1‑140 (MQ=255)
                   ttGCTAGAGCTTGTTCGTGTTTTCCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCtt                                                                                                       >  1:117046/1‑140 (MQ=255)
                                 tCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTACCAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAAt                                                                                         >  1:188530/1‑140 (MQ=255)
                                           cTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGATTATCAATAACATTAAGAAGTGGTGAAAGTTCATCATTGTTCTTTAGGTCTTTCAAATTTATAGCCTa                                                                               >  2:138732/1‑140 (MQ=255)
                                                     atatGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAAATGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTGGGTCTTTCCAATTTATAGCCTACGacacacca                                                                     >  2:298417/1‑136 (MQ=255)
                                                        tgtgATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCAAGCACAGt                                                                  >  1:106770/1‑140 (MQ=255)
                                                                 aGCGTGAATAATAATGCCTAATGTAACAAACCTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCCTTCCAATTTATAGCCTAAGCAACGCGCAGTCTtaaacag                                                         >  2:27070/1‑136 (MQ=255)
                                                                 aGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAg                                                         <  2:274489/140‑1 (MQ=255)
                                                                  gCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGGCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAAGAAGt                                                        >  2:233034/1‑140 (MQ=255)
                                                                   cGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGtt                                                       <  2:297489/140‑1 (MQ=255)
                                                                                     aTGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTTGGATTAt                                     >  1:13067/1‑140 (MQ=255)
                                                                                                             taCTGCTTATCAATAACATTAAGCCGTTGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGAc             <  1:308474/140‑1 (MQ=255)
                                                                                                              aCTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACt            >  1:83531/1‑140 (MQ=255)
                                                                                                                   ttATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGatat       <  1:320578/140‑1 (MQ=255)
                                                                                                                        aaTAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACa  >  2:282938/1‑140 (MQ=255)
                                                                                                                        aaTAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACa  <  2:106770/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                              
ATATATGATCTTGCATCTGTTGCTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCGGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACA  >  CP000730/1797639‑1797898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: