BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,913	C→G	18.9%	S213S (TCG→TCC)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,913	0	C	G	18.9%	272.5 / 73.9	171	S213S (TCG→TCC)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (44/92); new base G (12/20); total (56/115)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

TGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCT  >  CP000730/35775‑36052

tGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCg                                                                                                                                            <  2:220545/140‑1 (MQ=255)

tGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTACCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCGAATTTCGTGGCATGGCg                                                                                                                                            <  1:20256/140‑1 (MQ=255)

  aaCAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaa                                                                                                                                          <  2:133742/140‑1 (MQ=255)

    cAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaaaa                                                                                                                                        <  1:58740/140‑1 (MQ=255)

    cAAGAGCTAAAATAAAATTGAGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATCTCAGCGCTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaaaa                                                                                                                                        <  1:168685/140‑1 (MQ=255)

       gagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGGTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTCCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                                                                                                                     <  2:282614/140‑1 (MQ=255)

              aataaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATc                                                                                                                              >  2:197484/1‑140 (MQ=255)

              aataaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATc                                                                                                                              <  2:26774/140‑1 (MQ=255)

               acaaaagagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGa                                                                                                                           >  1:277841/15‑140 (MQ=11)

                taaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACGACTAATTTATCATGTCAGTATTCTCTTAACTGGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTg                                                                                                                            >  2:113808/1‑140 (MQ=255)

                caaaatagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                                                                                          <  1:49559/127‑1 (MQ=11)

                     gggtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                                                     <  2:299989/132‑1 (MQ=11)

                     gagtattttaataaAAAT‑GTTGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCCTAGATCTGAAGAGAc                                                                                                                     <  1:236721/132‑1 (MQ=11)

                     gagtattttaataaAAAT‑GATGTTTCCGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATTTATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                                                     <  2:8619/132‑1 (MQ=11)

                     gagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                                                     <  2:185617/132‑1 (MQ=11)

                     gagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCATAGATCTGAAGAGAc                                                                                                                     <  1:237699/132‑1 (MQ=11)

                     aagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAACATATTGCTATTCACTAATATGGCATGTCACTGTTCTTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                                                     <  2:266379/132‑1 (MQ=11)

                      agtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTTATCACTAATATATCATTTCAGTGTTCGTTTAACTTGCTAGTATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                                                    <  2:244745/133‑1 (MQ=11)

                      agtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                                                    <  2:114766/133‑1 (MQ=11)

                      agtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGATTTCGTGAGGGGAATTTCGTTGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                                                    <  2:307361/133‑1 (MQ=11)

                        tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTGTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                                                                  <  2:35041/135‑1 (MQ=11)

                        tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGATAATCACTAATATATCATGTCAGTTTTCGTTTAACTTGCTAGCATGATTCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                                                                  <  2:98734/135‑1 (MQ=11)

                         attttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATAGCATGTCAGTGGTCGTTTAACTTGCTAGCATGATGCTAGTTTCGTGGCATGGCGAAGATCCGTAGATCTGAAGAGCCCtcc                                                                                                                 >  1:93107/5‑138 (MQ=11)

                           tttaataataaT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATAGATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCTAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                               <  2:73333/138‑1 (MQ=11)

                            ttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AGAAAATATAGCTAATCACTAATTTATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                                                              >  2:155442/2‑140 (MQ=11)

                            ttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATTCGTAGATTCGAAGAGACCTGCGGt                                                                                                              >  1:246102/2‑140 (MQ=11)

                            cTAATGAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAGTTTATCATGTCAGTGTTCGCTTAACTTGCTACCATCCTGCTAATCTCGTGGCATGGCGCAAATCCGTAGATCTCACGACACCTGcc                                                                                                                >  2:126993/1‑139 (MQ=14)

                                       tcgttgtgcTGTTGCAAAGGAACAAACGATACATGCCCGCTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat                                                                                                     <  1:59144/132‑1 (MQ=1)

                                                                         aatcACTAATATAACATGTCAGTGTTCGTTTAACTTGCGAGCATGATGCTAATTTCGTGGCATTGCGAAAATCCGTAGATCTGAAGAGATCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGt                                                                   <  2:231575/137‑1 (MQ=1)

                                                                              ctaagatATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCt                                                              <  1:162449/134‑1 (MQ=1)

                                                                                             attgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                               <  1:227589/138‑1 (MQ=11)

                                                                                             aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc                                               <  1:319556/140‑1 (MQ=11)

                                                                                             aGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGGGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCTGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAACGTATTCTTTGCTGTATTTATAc                                               >  2:238705/1‑140 (MQ=11)

                                                                                             aGTGTTCGCTTAACCTGCTAGCATGATGCCAATTTTGCAGCTTTTCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAACTATTCTTTGCTGTATTGATAc                                               <  1:166207/140‑1 (MQ=11)

                                                                                               tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                             >  1:286994/1‑140 (MQ=17)

                                                                                               tgtTCGCTTAATTTCCTAGCATGATGCGAATTTCGTGTCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTGTATACAGGGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                             <  2:88842/140‑1 (MQ=11)

                                                                                                gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTCTTCTTTGCCGTATTGATACttt                                            >  1:132465/1‑140 (MQ=11)

                                                                                                 ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg                                           <  1:118661/140‑1 (MQ=11)

                                                                                                  tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                          <  1:39695/140‑1 (MQ=16)

                                                                                                  tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTATTTGCTGTATTGATACTTTgc                                          >  1:14048/1‑139 (MQ=255)

                                                                                                  tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                          <  1:25638/140‑1 (MQ=18)

                                                                                                  gtgCTTAACTTGCTATGATGATTCTAAGTTCGTGGCATGGCGAAAATTCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                          <  1:109477/138‑1 (MQ=11)

                                                                                                   tgCTTAACTTGCTAGCATGTTGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                         <  1:23496/139‑1 (MQ=16)

                                                                                                   cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCATATTGATACTTTGAt                                         >  1:13529/4‑140 (MQ=11)

                                                                                                   cgtttAACTTGCTAGCATGATGCTAATTTCGTGGAATGGCGAACATCCGAAGATCTGAAGAGAACTGCGATTCTTTTTATATAGGGCGTAAATACACTCAATCCCTTTTAAAGTATCCTTTGCTGTATTGATACTTTGaa                                         >  2:167449/4‑139 (MQ=11)

                                                                                                    gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATa                                        >  1:266889/3‑140 (MQ=255)

                                                                                                     tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTAATAc                                       >  1:107832/2‑140 (MQ=12)

                                                                                                     cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTACAGTATTCTTTGCTGTATTGATACTTTGATAc                                       <  2:226630/140‑1 (MQ=18)

                                                                                                     cTTAACTTGCTAGCATGATGCGAATTGCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                       <  2:246809/140‑1 (MQ=18)

                                                                                                     cTTAACTTGCTAGCATGAGGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                       <  1:160751/140‑1 (MQ=17)

                                                                                                      ttAACTTTCAAGCATGGTGATAAGTTCGTGGCATGGCGAAAATCCCTAGATCTGAAGAGACCTGCCGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAATGTATTCTTTGCTGTATTGATACTTTGATAcc                                      <  2:106928/140‑1 (MQ=11)

                                                                                                      ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                      <  2:240493/140‑1 (MQ=18)

                                                                                                      ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                      >  1:208182/1‑140 (MQ=17)

                                                                                                      gtAACTAGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTAttgataccttgatacc                                      <  2:309607/139‑1 (MQ=12)

                                                                                                        aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                    <  2:295305/140‑1 (MQ=18)

                                                                                                        aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCGGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                    <  2:110445/140‑1 (MQ=18)

                                                                                                          ctgtcTAGCATGATGCTAATTTCGTGGCATGGGGTAGTTCAGTGGAGCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGt                                  <  2:66778/136‑1 (MQ=11)

                                                                                                          cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGCGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                  <  2:47828/140‑1 (MQ=18)

                                                                                                          cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                  >  1:157348/1‑140 (MQ=32)

                                                                                                          cTTGCTAGCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                  <  1:197040/140‑1 (MQ=17)

                                                                                                           ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                 <  1:51826/140‑1 (MQ=18)

                                                                                                           ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                 >  1:280606/1‑140 (MQ=17)

                                                                                                            tGCTAGCATGATGCTAATTTCGTGGCATGGGGAAACTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtct                                <  1:177573/140‑1 (MQ=11)

                                                                                                            tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                <  1:141609/140‑1 (MQ=18)

                                                                                                             gCTCGCCTGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGCGCGTCCGGTTTCACAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTCtctt                               <  1:218199/140‑1 (MQ=11)

                                                                                                             gCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctt                               >  1:271302/1‑140 (MQ=11)

                                                                                                             gCTAGCATGATGCTAATTTCGTAGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                               <  1:102941/140‑1 (MQ=18)

                                                                                                             gCTAGCATGATGCTAAATTCGTGGCATGGCGAAAATCCGTAGATCTGAAAAGACCTGCGGTTCTCTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                               >  2:313803/1‑140 (MQ=12)

                                                                                                              cTAGCATGATGCTAATTTCGTGGCATTGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTCTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtcttt                              <  2:51739/140‑1 (MQ=11)

                                                                                                               tAGCATGATGCTAATTTCGTGGCATGGTTATTATCCGTAGATTTGAAGAGTCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                             <  1:7122/140‑1 (MQ=11)

                                                                                                               tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                             >  1:24053/1‑140 (MQ=32)

                                                                                                               caGCCTGATGCTAATTTCGTGGAATGGCGAAACTCCGCAGATCTCCAGTCGCGTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                             <  1:24624/139‑1 (MQ=11)

                                                                                                                aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                            <  1:289956/140‑1 (MQ=17)

                                                                                                                  cATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTa                          >  2:16155/1‑140 (MQ=11)

                                                                                                                  cATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAATTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                          <  2:32981/140‑1 (MQ=16)

                                                                                                                  cATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                          >  2:205673/1‑140 (MQ=17)

                                                                                                                   atgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAAACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTAc                         >  2:89885/1‑140 (MQ=18)

                                                                                                                     gatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                       >  1:311662/1‑140 (MQ=255)

                                                                                                                      atgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACttt                      <  2:166949/140‑1 (MQ=11)

                                                                                                                      atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAGATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                      >  1:57545/1‑140 (MQ=19)

                                                                                                                      atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                      >  1:119133/1‑140 (MQ=21)

                                                                                                                      atgCTAAGTTCGTGGCATGGTGAAAATCCCTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATATATTCTATTTTTTTTTGTGGATTCTTTGCCGTATTGATACTTTGATATCTTTTCTTTCTTACttt                      <  2:67928/140‑1 (MQ=11)

                                                                                                                        gCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCAACAATTATTATTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACCTTaa                    <  1:217941/140‑1 (MQ=11)

                                                                                                                        gCTAATTTCGTGGCATGGCGAAAATCCGTCGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTaa                    <  1:91255/140‑1 (MQ=255)

                                                                                                                        gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTaa                    >  1:174045/1‑140 (MQ=37)

                                                                                                                         cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                   <  1:295509/140‑1 (MQ=37)

                                                                                                                         cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                   <  1:295498/140‑1 (MQ=37)

                                                                                                                          tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                  >  2:28785/1‑140 (MQ=21)

                                                                                                                            aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATg                <  1:287539/140‑1 (MQ=11)

                                                                                                                            aTTTCGTGGCATGGCGAAAATCTGGAGATCTGAAGAGACCTGCGGTTCTTTTAATATAGAGCGTAGATACATTCAATACCTTTTAAAATAATCTTTGCTGTATTGATACTTTGATGCCTTGtatgtctaccatttattta                >  2:168844/1‑128 (MQ=11)

                                                                                                                              ttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTCTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGAc              <  2:209713/140‑1 (MQ=11)

                                                                                                                              ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGAc              >  1:18653/1‑140 (MQ=21)

                                                                                                                               tCGTGGCATGGCGAAAATCCGGATATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg             <  2:313604/140‑1 (MQ=255)

                                                                                                                                  tGGCATGGCGAAAATCCGTAGCTCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTGAATATGACGGTg          <  2:195231/140‑1 (MQ=255)

                                                                                                                                   ggCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGa         <  1:148463/140‑1 (MQ=14)

                                                                                                                                   ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa         >  1:282450/1‑140 (MQ=255)

                                                                                                                                     cATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATc       >  2:144391/1‑140 (MQ=37)

                                                                                                                                     cATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCCTTCTTACTTTAATATGACGGTGATc       >  1:215122/1‑140 (MQ=255)

                                                                                                                                       tGGCGAAAATCCGTAGATCTGTAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTGTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTGCTTACTTTAATATGACGGTGATCtt     <  2:79966/140‑1 (MQ=255)

                                                                                                                                          cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt  <  2:93844/140‑1 (MQ=38)

TGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCT  >  CP000730/35775‑36052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`