Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,866	G→T	68.2%	intergenic (‑949/‑219)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,866	0	G	T	68.2%	63.2 / 73.9	88	intergenic (‑949/‑219)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (16/10);  new base T (32/24);  total (51/37)
Fisher's exact test for biased strand distribution p-value = 8.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-02

AGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGC‑CTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTA  >  CP000730/1929730‑1929999                                                                                                                                            |                                                                                                                                        aGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCGAGTATATTTGCCGATGATATAATAGTAGAGTCGC‑CTCTCTCTCAGGCGTCAATTTCGACGCAGAGAGGAGGGGTA‑TAA‑G‑G‑Tg                                                                                                                                    >  2:152419/1‑140 (MQ=255)              tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGC‑CTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCg                                                                                                                       <  1:51659/140‑1 (MQ=255)                tCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGC‑CTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGtt                                                                                                                     >  1:178377/1‑140 (MQ=255)                        aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGC‑CTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCAcatcat                                                                                                             <  2:297655/140‑1 (MQ=255)                                aaCTTATCAGAAGCAATTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTAGC‑CTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAg                                                                                                     <  1:273982/140‑1 (MQ=255)                                       cAGAAGCAAGTGACTGTGCTTGTATATTTACCGATGATATAATAGTAGAGTCGC‑CTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGAACCAGTCATCAg                                                                                              >  2:86032/1‑140 (MQ=255)                                            ggaagtagatatgCTTGTATGTTCACAGCTGGTATAATAATAGAATCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTGCACATCATAGCACCAGTCATCAGTGGCtg                                                                                        >  2:175165/12‑140 (MQ=255)                                            ggaagtagatatgCTTGTATGTTCACAGATGGTATAATAATAGATTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTGTCGTTCACAGCATAGCACCAGTCATCAGTGGcgg                                                                                        >  2:175161/12‑138 (MQ=255)                                            ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATCGAGTCGC‑ATATCTCTCGGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCGGTGGCtg                                                                                        >  1:84332/12‑140 (MQ=255)                                            ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                        <  2:251844/129‑1 (MQ=255)                                            ggaagtagataggCTTGTATATTCACAGATGGTATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                        <  2:122064/128‑1 (MQ=255)                                            gCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGC‑CTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTACGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                         >  2:178940/1‑140 (MQ=255)                                             gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                       >  2:109822/11‑140 (MQ=255)                                             gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                       <  1:250714/130‑1 (MQ=255)                                             gaagtagatatgCTTGTATATTCACAGATGGTATAAGAATAGAGGCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                       <  2:44671/130‑1 (MQ=255)                                              aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                      <  2:221723/131‑1 (MQ=255)                                              aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTCCGTTCACATCATACCACCAGTCATCAGTGGCcgtg                                                                                      >  1:28533/10‑140 (MQ=255)                                              aagtagatatgCTTGTATATTCACAGATGGTATAAAAATAGAG‑CGCTGAAGCTCCCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                      <  1:24688/131‑1 (MQ=255)                                              aagtagatatgCTTGTATATTCACAGACGGTATAATAATAGAGGCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGAATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                      <  1:295832/131‑1 (MQ=255)                                                gtaggtatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                    >  2:194116/8‑139 (MQ=255)                                                gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                    <  1:40409/133‑2 (MQ=255)                                                 tatctatgCTTGTATATTCACACATGGTATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                   <  2:289206/134‑3 (MQ=255)                                                 tatatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                   <  2:57970/134‑3 (MQ=255)                                                 tGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGC‑CTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGGTTATTTTCGTTCACATCATAGCACCAGTCATCAATGGCTGTGcc                                                                                    >  2:51782/1‑140 (MQ=255)                                                    atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                >  2:299884/4‑135 (MQ=255)                                                     tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                                                               >  2:297183/3‑134 (MQ=255)                                                     aatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCAACAGTCATCAGTGGCTGTGctgtttc                                                                               >  2:133674/3‑134 (MQ=255)                                                        gtttctataCTCGCAGATGCTAAAATCGTAGTGTCGC‑CTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑A‑G‑TGTTTCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGt                                                                             <  1:139485/135‑1 (MQ=255)                                                         cTTGTATATTTGCCGATGATATAATAGTAGAGTCGC‑CTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGGGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCCGTCATCATTGGCTGTGCCATTGCGtt                                                                            >  2:106957/1‑140 (MQ=255)                                                         cTTGTATATTTGCCGATGATATAATAGTAGAGTCGC‑CTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGAGGATGTGCCATTGCGtt                                                                            >  2:264549/1‑140 (MQ=255)                                                          ttGTATATTCACAGATGGTATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                          <  2:164329/140‑4 (MQ=255)                                                            gTATATTTGCCGATGATATAATAGTAGAGTCGC‑CTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                                                                         >  1:251582/1‑140 (MQ=255)                                                                atTTGCCGATGATATAATAGTATAGTCGC‑CTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑CGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCt                                                                     <  1:81347/140‑1 (MQ=255)                                                                atTTGCCGAGGATACAATAGTAGAGTCGC‑CTATTTCACAGGCGTCAACTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑GGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCt                                                                     <  1:81341/140‑1 (MQ=255)                                                                  tcacagatggtATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                  >  1:128815/6‑140 (MQ=255)                                                                    acagatggtATAATAATAGAGACGC‑CTATCTCTCAGGCGTAAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTACTCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTCTGCTGTTGCGTATTCTAATTATTg                                                                >  2:266247/4‑140 (MQ=255)                                                                       gatggtATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtttactaagccaataag                                                             <  2:196909/140‑15 (MQ=255)                                                                       gatggtATAATAATAGAGTCGC‑CTAGCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                             <  1:170002/140‑1 (MQ=255)                                                                       gatgatATAATAGTAGAGGCGC‑CTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                              <  1:38697/140‑1 (MQ=255)                                                                        atggtataATAATAGTGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACA‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                            >  1:238649/5‑139 (MQ=255)                                                                        atggtATAATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATCCTTAATGGCtt                                                            >  2:11187/1‑139 (MQ=255)                                                                         tggtataATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta                                                           <  2:313900/137‑3 (MQ=255)                                                                         tggtataATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta                                                           >  1:182508/4‑138 (MQ=255)                                                                         tgatATAATAGTAGAGTCGC‑CTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTa                                                            >  1:292239/1‑140 (MQ=255)                                                                          ggtataATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCAGATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttat                                                          >  1:84081/3‑137 (MQ=255)                                                                           gtataATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                         <  2:298587/139‑1 (MQ=255)                                                                           gtataATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                         >  1:77348/2‑140 (MQ=255)                                                                           gtataATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTAGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                         >  1:92515/2‑140 (MQ=255)                                                                           gtataATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                         >  1:37199/2‑140 (MQ=255)                                                                            tataATAATAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                        >  2:74675/1‑140 (MQ=255)                                                                             ataataataGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttaataa                                                       >  1:164251/1‑134 (MQ=255)                                                                             ataataataGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCAGAGCACCCGTCATCAGTGGCTGTGCGGTTGCGTATTATACTTATTGACTTAGTat                                                       >  1:133564/1‑139 (MQ=255)                                                                              tgataataGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                      <  1:268648/138‑3 (MQ=255)                                                                              taataaTAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                      <  1:204825/140‑3 (MQ=255)                                                                               aataatAGAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                     <  1:104433/140‑4 (MQ=255)                                                                                 taatagAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                   <  1:170102/137‑1 (MQ=255)                                                                                  aatagAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                  <  2:252960/138‑1 (MQ=255)                                                                                   atagAGTCGC‑TTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCCTCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                 <  2:164986/139‑1 (MQ=255)                                                                                   atagAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTCAACGCaa                                                 >  2:85444/2‑140 (MQ=255)                                                                                   atagAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                 >  1:205405/2‑140 (MQ=255)                                                                                   atagAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGGGCTGTTGCGTATTATACTTCTTGGCTTGGCAAACGCaa                                                 >  2:212947/2‑140 (MQ=255)                                                                                    tagAGTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                                >  1:144203/1‑139 (MQ=255)                                                                                     agagTCGC‑CTATCTCTCAGGCGTCAATTT‑‑ACCAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                               <  1:121148/140‑3 (MQ=255)                                                                                     agagTCGC‑CAATCTGTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                               <  2:33139/140‑3 (MQ=255)                                                                                     agagTCGC‑ATATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGGGCAGTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCATCAGTCATCAGTTGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                               <  1:27602/140‑3 (MQ=255)                                                                                      gagTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                              >  1:95260/1‑137 (MQ=255)                                                                                       agTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                             >  1:305515/1‑136 (MQ=255)                                                                                        gTCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                            >  1:257922/1‑135 (MQ=255)                                                                                         tCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTGTACTTATTGGCTTAGTAAACGCaacaactg                                           >  1:36822/1‑134 (MQ=255)                                                                                         tCGC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                           >  1:260918/1‑134 (MQ=255)                                                                                           gC‑CTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGACATCAGTGGCTGTGCTGTTGCGTATTATACGTATTGTCTTGATAAACGCaacaaataat                                         >  2:15509/1‑132 (MQ=255)                                                                                            c‑cTATCTCTCAGGCGTCAATTTAGACGCAGAGAAGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCGTCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                                         >  2:211193/1‑140 (MQ=255)                                                                                            c‑cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                        >  2:23679/1‑131 (MQ=255)                                                                                              cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                       <  2:24899/140‑11 (MQ=255)                                                                                                aTCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATaag                                      >  1:270241/1‑138 (MQ=255)                                                                                                aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                     >  2:149962/1‑128 (MQ=255)                                                                                                 tctctcAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGt                                     >  1:121041/1‑140 (MQ=255)                                                                                                 tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                    >  2:312557/1‑127 (MQ=255)                                                                                                  ctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                   <  2:25427/140‑15 (MQ=255)                                                                                                  ctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                   >  2:21647/1‑126 (MQ=255)                                                                                                   tctcAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACCCAATAGGCGa                                   >  1:128950/1‑140 (MQ=255)                                                                                                   tctcAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGATGa                                   >  1:128977/1‑140 (MQ=255)                                                                                                       aGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata                               <  2:292239/140‑1 (MQ=255)                                                                                                           gTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACACAATAGGTGACATATAGc                           >  2:76128/1‑140 (MQ=255)                                                                                                                tttAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCCCTACAAAATAGGTTTCATAAAGCCGccc                      >  1:262854/1‑138 (MQ=255)                                                                                                                            gaggagGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCATATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATcc          <  2:135504/140‑1 (MQ=255)                                                                                                                              ggaggTGTA‑TAA‑G‑G‑TGTTGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATcccc        <  1:29137/140‑1 (MQ=255)                                                                                                                                    gtA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTa  <  1:304720/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                        AGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGC‑CTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTA  >  CP000730/1929730‑1929999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑