Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,470,963 T→A 18.2% Y291F (TAC→TTC)  USA300HOU_2333 ← MFS family major facilitator transporter, multidrug:cation symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,470,9630TA18.2% 41.2 / 5.4 23Y291F (TAC→TTC) USA300HOU_2333MFS family major facilitator transporter, multidrug:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (9/9);  new base A (2/2);  total (12/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01

TCGTACTAAAAATGATACCTGGGATATCAGCTTTAGGGTTTGTTGTATATTGATATAACTTAAACCATACAAAACCAATTAAAATAGCGATAATCCCGATAATGAACATACCGTAAAACATCACATTCCAATGGTAATTTTGTACAATATAACCTGATAATGTTGGACCAATTGCAGGTGCTAAAATCATTGCGATACCCATTGTACCCATGGCAGCACCACGTTTTTCAGGTGGATAAATTGTAATAATAACAATTGAACCTAA  >  CP000730/2470829‑2471093
                                                                                                                                      |                                                                                                                                  
tCGTACTAAAAATGATACCTGGGATATCAGCTTTAGGGTTTGTTGTATATTGATATAACTTAAACCATACAAAACCAATTAAAATAGCGATAATCCCGATAATGAACATACCGTAAAACATCACATTCCAATGGTAAttt                                                                                                                               <  1:51144/140‑1 (MQ=255)
          aaTGATAACTGGGATATCAGCTTTAGGGTTTGTTGTATATTGATATAACTTAAACCATACAAAACCAATTAAAATAGCGATAATCCCGATAATGAACATACCGTAAAACATCACATTCCAATGGTAATTTTGTACAatat                                                                                                                     >  2:300034/1‑140 (MQ=255)
                                 tAGGGTTTGTTGTATATTGATATAACTTAAACCATACAAAACCAATTAAAATAGCGATAATCCCGATAATGAACATACCGTAAAACATCACATTCCAATGGTAATTTTGTACAATATAACCTGATAATGTTGGACCAAtt                                                                                              <  1:90205/140‑1 (MQ=255)
                                        tgttgtATATTGATATAACTTAAACCATACAAAACCAATTAAAATAGCGATAATCCCGATAATGAACATACCGTAAAACATCACATTCCAATGGTAATTTTGTACAATATAACCTGATAATGTTGGACCAATTGCAGGTg                                                                                       >  1:248768/1‑140 (MQ=255)
                                           tgtATATTGATATAACTTAAACCATACAAAACCAATTAAAATAGCGATAATCCCGATAATGAACATACCGTAAAACATCACATTCCAATGGTAATTTTGTACAATATAACCTGATAATGTTGGACCAATTGCAGGTGCTa                                                                                    >  2:277676/1‑140 (MQ=255)
                                                   gATATAACTTAAACCATACAAAACCAATTAAAATAGCGATAATCCCGATAATGAACATACCGTAAAACATCACATTCCAATGGTAATTTTGTACAATATAACCTCATAATGTTGGAGCCATTGCAGGTGCTAAAATCAtt                                                                            >  1:15243/1‑140 (MQ=255)
                                                         aCTTAAACCATACAAAACCAATTAAAATAGCGATAATCCCGATAATGAACATACCGTAAAACATCACATTCCAATGGTAATTTTGTACAATATAAGCTGATAATGTTGGACCAATTGCAGGTGCTAAAATCATTGCGATa                                                                      >  2:296044/1‑140 (MQ=255)
                                                         aCTTAAACCATACAAAACCAATTAAAATAGCGATAATCCCGATAATGAACATACCGTAAAACATCACATTCCAATGGGAATTTTGTACCATATAACCTAATAATGATGGACCAATTGAGGGTGCTaaaacacttgccgta                                                                      >  2:293870/1‑129 (MQ=255)
                                                            tAAACCATAAAAAACCAATTAAAATATCGAAAATCCCCATACTGAACATACCGTAAACCATCACATTCCAATGGTAATTTTTTAAAATCTAACCTGCTAATGTTGGACCAATTGCAGGTGCTAAAATCATTGCGATAccc                                                                   <  2:47650/140‑1 (MQ=255)
                                                                       aaaCCAATTAAAATAGCGATAATCCCGATAATGAACATACCGTAAAACATCACATTCCAATGGTAATTTTGTACAATATAACCTGATAATGTTGGACCAATTGCAGGTGCTAAAATCATTGCGATACCCATTGTACCCAt                                                        <  1:219765/140‑1 (MQ=255)
                                                                         aCCAATTAAAATAGCGATAATCCCGATAATGAACATACCGTAAAACATCACATTCCAATGGAAATTTTGTACAATATAACCTGATAATGTTGGACCAATTGCAGGTGCTAAAATCATTGCGATACCCATTGTACCCATgg                                                      >  1:148252/1‑140 (MQ=255)
                                                                         aCCAATTAAAATAGCGATAATCCCGATAATGAACATACCGTAAAACATCACATTCCAATGGAAATTTTGTACAATATAACCTGATAATGTTGGACCAATTGCAGGTGCTAAAATCATTGCGATACCCATTGTACCCATgg                                                      >  1:236271/1‑140 (MQ=255)
                                                                               tAAAATAGCGATAATCCCGATAATGAACATACCGTAAAACATCACATTCCAATGGTAATTTTGTACAATATAACCTGATAATGTTGGACCAATTGCAGGTGCTAAAATCATTGCGATACCCATTGTACCCATGGCAGcac                                                >  2:315282/1‑140 (MQ=255)
                                                                                  aaTAGCGATAATCCCGATAATGAACATACCGTAAAACATCACATTCCCATGGTAATTTTGTACAATATAACCTGATAATGTTGGACCAATTGCAGGTGCTAAAATCATTGCGATACCCATTGTACCCATGGCAGcaccac                                             >  1:202688/1‑140 (MQ=255)
                                                                                          tAATCCCGATAATGAACATACCGTAAAACATCACATTCCAATGGTAATTTTGTACAATATAACCTGATAATGTTGGACCAATTGCAGGTGCTAAAATCATTGCGATACCCATTGTACCCATGGCAACACCACGTTTTTCa                                     >  1:174802/1‑140 (MQ=255)
                                                                                             tCCCGATTATGAACATACCGTAAAACATCACATTCAAATGCTAATTTTGTACAATATAACCTGACAATGAAGGACCCATTGAAGCTCCTAACGACACCGCCATACACAATCTAGCCGTGGCATCACCAGCGTTTGCAGGt                                  <  2:63703/140‑1 (MQ=255)
                                                                                              ccccATAATGAACATACCGTAAAACATCACATTCCAATGGTAATTTTGTACAATATAACCTGATAATGTTGGACCAATTGCAGGTGCTAAAATCATTGCGATACCCATTGTACCCATGGCAGCACCACGTTTTTCAGGTg                                 <  2:109753/140‑1 (MQ=255)
                                                                                              cccGATAATGAACATACCTTAAAACATCACATTCCAATGGTAATTTTGTACAATATAACCTGATAATGTTGGACCAATTGCAGGTGCTAAAATCATTGCGATACCCATTGTACCCATGGCAGCACCACGTTTTTCAGGTg                                 <  2:156281/140‑1 (MQ=255)
                                                                                                     aTGAACATACCGTAAAACATCACATTCCAATGGTAATTTTGTACAATATAACCTGATAATGTTGGACCCATTGCAGGTGCTAACATCATTGTGATACCCATTGTACCCATGGCAGCACACCATTTTTCAGGAGGATAAAt                          >  1:200747/1‑140 (MQ=255)
                                                                                                        aaCATACCGTAAAACATCACATTCCAATGGTAATTTTGTACAATATAACCTGATAATGTTGGACCAATTGCAGGTGCTAAAATCATTGCGATACCCATTGTACCCATGGCAGCACCACGTTTTTCAGGTGGATAAATTGt                       <  2:227913/140‑1 (MQ=255)
                                                                                                                gTAAAACATCACATTCCAATGGTAATTTTGTACAATATAACCTGATAATGTTGGACCAATTGCAGGTGCTAAAATCATTGCGATACCCATTGTACCCATGGCAGCACCACGTTTTTCAGGTGGATAAATTGtaataataa               <  2:199844/140‑1 (MQ=255)
                                                                                                                             ttCCAATGGAAATTTTGTACAATATAACCTGATAATGTTGGACCAATTGCAGGTGCTAAAATCATTGCGATACCCATTGTACCCATGGCCGCACCACGTTTTTCAGGTGGATAAATTGTAATAATAACAATTGAACCTaa  <  2:236271/140‑1 (MQ=255)
                                                                                                                             ttCCAATGGAAATTTTGTACAATATAACCTGATAATGTTGGACCAATTGCAGGTGCTAAAATCATTGCGATACCCATTGTACCCATGGCAGCACCACGTTTTTCAGGTGGATAAATTGTAATAATAACAATTGAACCTaa  <  2:148252/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
TCGTACTAAAAATGATACCTGGGATATCAGCTTTAGGGTTTGTTGTATATTGATATAACTTAAACCATACAAAACCAATTAAAATAGCGATAATCCCGATAATGAACATACCGTAAAACATCACATTCCAATGGTAATTTTGTACAATATAACCTGATAATGTTGGACCAATTGCAGGTGCTAAAATCATTGCGATACCCATTGTACCCATGGCAGCACCACGTTTTTCAGGTGGATAAATTGTAATAATAACAATTGAACCTAA  >  CP000730/2470829‑2471093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: