Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,532,2980AT100.0% 53.5 / NA 18T15T (ACA→ACTUSA300HOU_2392FemAB family peptidoglycan biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (5/13);  total (5/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAG  >  CP000730/2532165‑2532431
                                                                                                                                     |                                                                                                                                     
tATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                 <  2:1203/140‑1 (MQ=255)
 agttGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGc                                                                                                                                <  1:11522/138‑1 (MQ=255)
     gTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTCCAAAGCAttt                                                                                                                            >  1:128519/1‑140 (MQ=255)
                 tGAAAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                                <  2:205856/140‑1 (MQ=255)
                      aaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAAt                                                                                                           <  2:183554/140‑1 (MQ=255)
                       aataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATc                                                                                                          <  1:34299/140‑1 (MQ=255)
                          aaaaaaaTAGCATAACTAATAAACTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGc                                                                                                       <  1:172581/140‑1 (MQ=255)
                                        attaATAAATTCAAAAATTAGAGTTCAATTCAGAGAGGGATGGGGTAAAATGGACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTACACATTATCCACAACCAGATATCCATTACAATc                                                                                         >  1:194813/1‑140 (MQ=255)
                                           aataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATa                                                                                      <  2:202975/140‑1 (MQ=255)
                                                   cAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGAt                                                                              >  2:232683/1‑140 (MQ=255)
                                                        aTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaaa                                                                         <  1:206407/140‑1 (MQ=255)
                                                                 cAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtgt                                                                <  2:256222/140‑1 (MQ=255)
                                                                     tCAGTGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCAt                                                            <  1:280908/140‑1 (MQ=255)
                                                                     tCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCAt                                                            <  2:151397/140‑1 (MQ=255)
                                                                     tCAGAGAGGGATGGGATAAAATGAACTCCGTCATGTTGATTCAGGACGAGAAATATGAGTTTTCTAATGAGCATGTTTCGCATTACACTCGATCGTCTAGTCTTTACGGGCATTGAGTTGATTTAAAAGGCGATGTGCAt                                                            <  2:153952/140‑1 (MQ=255)
                                                                         agagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTTCATCTTg                                                        >  2:318883/1‑140 (MQ=255)
                                                                                                                   tGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGGGATTGCAGGATGCt              >  2:259223/1‑140 (MQ=255)
                                                                                                                               gTTTACTACCAAGCAATTATCACATTTCACACAATCAGCTATTCATTACTATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAGAGATGACAATGGGCAAGTGATTGCAGTATGCTTATTGACAGAAg  <  2:209896/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
TATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAG  >  CP000730/2532165‑2532431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: