BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,997	T→C	27.3%	T185T (ACA→ACG)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,997	0	T	C	27.3%	531.5 / 275.8	396	T185T (ACA→ACG)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (143/143); new base C (63/45); total (207/189)
Fisher's exact test for biased strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01

Only 100 of 399 total aligned reads displayed.

AGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCT  >  CP000730/35868‑36136

attgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                                                                                   <  1:227589/138‑1 (MQ=11)

aGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGGGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCTGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAACGTATTCTTTGCTGTATTTATAc                                                                                                                                   >  2:238705/1‑140 (MQ=11)

aGTGTTCGCTTAACCTGCTAGCATGATGCCAATTTTGCAGCTTTTCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAACTATTCTTTGCTGTATTGATAc                                                                                                                                   <  1:166207/140‑1 (MQ=11)

  tgtTCGCTTAATTTCCTAGCATGATGCGAATTTCGTGTCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTGTATACAGGGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                                                                                 <  2:88842/140‑1 (MQ=11)

   gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTCTTCTTTGCCGTATTGATACttt                                                                                                                                >  1:132465/1‑140 (MQ=11)

   gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                                                                                                                >  1:284324/1‑140 (MQ=11)

   gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                                                                                >  1:81541/1‑140 (MQ=12)

     tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                                                                              <  1:39695/140‑1 (MQ=16)

     tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                                                                              <  1:25638/140‑1 (MQ=18)

     gtgCTTAACTTGCTATGATGATTCTAAGTTCGTGGCATGGCGAAAATTCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                                                                              <  1:109477/138‑1 (MQ=11)

      tgCTTAACTTGCTAGCATGTTGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                                                                             <  1:23496/139‑1 (MQ=16)

      cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCATATTGATACTTTGAt                                                                                                                             >  1:13529/4‑140 (MQ=11)

      cgtttAACTTGCTAGCATGATGCTAATTTCGTGGAATGGCGAACATCCGAAGATCTGAAGAGAACTGCGATTCTTTTTATATAGGGCGTAAATACACTCAATCCCTTTTAAAGTATCCTTTGCTGTATTGATACTTTGaa                                                                                                                             >  2:167449/4‑139 (MQ=11)

         ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                                                          >  1:208182/1‑140 (MQ=17)

          gaaCTTGCTAGGATGATGCTAATGTCGTGGCATGGCGAAAAGCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAGGACACTCAATAGCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                                                                         <  2:208840/139‑1 (MQ=11)

           aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCGGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                                                                        <  2:110445/140‑1 (MQ=18)

              ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                                                     >  1:280606/1‑140 (MQ=17)

                catAGTATGATGTTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCATTTTATAAAGTGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                                                                                   <  1:180358/138‑1 (MQ=11)

                  tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                                                 >  1:24053/1‑140 (MQ=32)

                  caGCCTGATGCTAATTTCGTGGAATGGCGAAACTCCGCAGATCTCCAGTCGCGTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                                                 <  1:24624/139‑1 (MQ=11)

                   aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                                                <  2:284879/140‑1 (MQ=18)

                     cATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTa                                                                                                              >  2:16155/1‑140 (MQ=11)

                        gatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                                                                           >  1:311662/1‑140 (MQ=255)

                         atgCTAATTTCGTGGCATGGGGAACATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACttt                                                                                                          <  2:107503/140‑1 (MQ=11)

                         atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                                                          <  1:50244/140‑1 (MQ=37)

                         atgCTAAGTTCGTGGCATGGTGAAAATCCCTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATATATTCTATTTTTTTTTGTGGATTCTTTGCCGTATTGATACTTTGATATCTTTTCTTTCTTACttt                                                                                                          <  2:67928/140‑1 (MQ=11)

                                  tCGTGGCATGGCGAAAATCCGGATATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg                                                                                                 <  2:313604/140‑1 (MQ=255)

                                    gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                                               >  2:155918/1‑140 (MQ=255)

                                        cATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCCTTCTTACTTTAATATGACGGTGATc                                                                                           >  1:215122/1‑140 (MQ=255)

                                           ggggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                                                        <  1:268909/137‑1 (MQ=255)

                                            gCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGCTCTTGc                                                                                       >  2:60980/1‑140 (MQ=255)

                                             cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                                                                                      <  2:93844/140‑1 (MQ=38)

                                              gAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCCTTGCTGTATAGATACTTTGATACCTTGTCTTTCTTCCATGAATAGGACGGTGATCTTGCTc                                                                                     <  2:227385/140‑1 (MQ=11)

                                                aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTGTTTCTTACTGTAATCTCTCGGTGACCCTGCGCat                                                                                   >  1:103557/1‑139 (MQ=11)

                                                aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCaa                                                                                   <  2:263679/140‑1 (MQ=255)

                                                  aTCCGTAGATCTGAAGGCACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATACGACGGTGATCTTGCTCAATg                                                                                 <  1:101866/140‑1 (MQ=255)

                                                    ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATCAACTAGTGACCTTTTACAGACTGTGGTTCTGTATTGATCCTTTGCAACCTGGTCTTTCTTAGTTTAATATGACGGTGATCGTGCTGAATGAg                                                                               >  1:153772/1‑140 (MQ=2)

                                                      gTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTTAATGGGGt                                                                             >  1:108184/1‑140 (MQ=255)

                                                            cTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCa                                                                       >  1:115681/1‑140 (MQ=255)

                                                              gAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                                                     <  1:16155/140‑1 (MQ=255)

                                                               aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCCTGCTCAATGAGGTTCTTCAGat                                                                    >  2:51092/1‑140 (MQ=255)

                                                                    aCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGATTATTCAAATATTTc                                                               >  1:149169/1‑140 (MQ=11)

                                                                      cTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa                                                             <  1:163608/140‑1 (MQ=255)

                                                                       tGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCCATGAGGTTATTCAGATATTTCGAt                                                            >  2:84747/1‑140 (MQ=255)

                                                                       tGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTTAGATATTTTGAt                                                            >  1:85496/1‑140 (MQ=255)

                                                                       tGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt                                                            >  1:127203/1‑140 (MQ=255)

                                                                        gCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATg                                                           >  2:212559/1‑140 (MQ=255)

                                                                         cGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                          >  2:256734/1‑140 (MQ=255)

                                                                           gTTCTTTGTATATATATCGTAAATACACTCAATACCTGTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTGTCTTACTTTAATATGACGGTGATCTCGCTCAATGTGGTTATTCTGATATTTCGATGTAc                                                        <  2:248955/140‑1 (MQ=11)

                                                                           gTTCTTTCTATATAGAGAGTAAATACATTCAATACCTTTAAAAGGATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACTGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTAc                                                        <  1:143353/140‑1 (MQ=11)

                                                                            ttCTTTTTATATAGCGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACa                                                       <  1:186521/140‑1 (MQ=255)

                                                                             tCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACaa                                                      >  2:220662/1‑140 (MQ=255)

                                                                               tttttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATg                                                    <  2:292795/140‑1 (MQ=255)

                                                                                ttttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATgg                                                   <  2:246102/140‑2 (MQ=255)

                                                                                 tttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGATGTTATTCAGATATTTCGATGTACATTggc                                                  >  2:265400/1‑138 (MQ=255)

                                                                                 tttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATggc                                                  <  1:119126/140‑3 (MQ=255)

                                                                                  ttATATAGAGCGTAAATAAACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCa                                                 >  2:115626/1‑140 (MQ=255)

                                                                                    atataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGt                                               >  1:172141/1‑140 (MQ=255)

                                                                                    atataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGt                                               <  2:47564/140‑1 (MQ=255)

                                                                                    atataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                                               <  1:251512/140‑1 (MQ=255)

                                                                                       taGTGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGCTCTTGCTCTATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAg                                            <  2:131773/140‑1 (MQ=14)

                                                                                       taGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAg                                            >  2:145451/1‑140 (MQ=255)

                                                                                        agagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCTATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAgg                                           >  1:298481/1‑140 (MQ=255)

                                                                                        agagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAgg                                           <  1:89885/140‑1 (MQ=255)

                                                                                          agCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtt                                         >  1:20122/1‑140 (MQ=255)

                                                                                              tAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag                                     <  1:266801/140‑1 (MQ=255)

                                                                                              tAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCATGCTCACTGCGGTTATCCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                     >  1:293193/1‑140 (MQ=14)

                                                                                              tAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAGACCTTGTCTTTGTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                     <  1:138362/140‑1 (MQ=14)

                                                                                              gaaataacctCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                     <  2:315386/131‑1 (MQ=255)

                                                                                               aaaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCGATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagt                                    >  1:73580/1‑140 (MQ=255)

                                                                                                aagtcactCAATACCTTTTAAAGTATGCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt                                   <  1:32208/133‑1 (MQ=255)

                                                                                                aaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACCATGACAGTCAGgtttaagtt                                   >  2:23052/1‑140 (MQ=255)

                                                                                                aaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtt                                   <  2:241696/140‑1 (MQ=255)

                                                                                                aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt                                   >  2:171692/1‑140 (MQ=255)

                                                                                                aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt                                   <  1:53787/140‑1 (MQ=255)

                                                                                                 atactctCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                  <  1:99398/134‑1 (MQ=255)

                                                                                                 aTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                  >  1:122716/1‑140 (MQ=255)

                                                                                                 aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGCTGTACAATGGCAGTCAGgtttaagttt                                  >  1:67383/1‑140 (MQ=255)

                                                                                                  tgcactCAATACCTTTTAAAGTATTCTTTGTTGTATTTATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                 <  1:278421/135‑1 (MQ=14)

                                                                                                  tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                 >  1:187693/1‑140 (MQ=255)

                                                                                                    cactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAGTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAAGTACACTGGCAGTCAGGGTTAAGTTTaaa                               >  2:200543/4‑140 (MQ=14)

                                                                                                    cATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCATTGATGTTATTCAGATACTTCGATGTACAATGACAGTCAGGTTTAAGTCTaaa                               <  1:218163/140‑1 (MQ=255)

                                                                                                    cATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTaaa                               >  2:105073/1‑140 (MQ=255)

                                                                                                      ctCAATACCTTTTAAAGTATTCTTTGATGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttgagtttataga                             >  2:151672/2‑136 (MQ=14)

                                                                                                         aaTACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCtt                          >  1:176263/1‑140 (MQ=255)

                                                                                                            aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTaa                       >  2:260943/1‑140 (MQ=255)

                                                                                                             ccTTTTAAAGTATGCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACCATGGAAGTCAGGTTAAAGTTTAAAAGCTTGAAt                      <  2:267865/140‑1 (MQ=14)

                                                                                                               ttttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                    >  1:196341/1‑140 (MQ=255)

                                                                                                                  tAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACtt                 <  1:289760/140‑1 (MQ=255)

                                                                                                                    aaGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTa               >  1:94926/1‑140 (MQ=255)

                                                                                                                     aGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAg              >  2:79525/1‑140 (MQ=255)

                                                                                                                       tATTCTTTGCTGTATTGATACTTTCTTACCGTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAAcc            >  2:308549/1‑140 (MQ=14)

                                                                                                                       tATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGcc            >  1:141200/1‑140 (MQ=255)

                                                                                                                        aTTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTGCGATGACAGTCAGGTTTAAGTGTGAAAGCTTTAGTTATTGTAGCCa           >  2:301686/1‑140 (MQ=14)

                                                                                                                         tccTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAt          >  2:277155/3‑140 (MQ=255)

                                                                                                                           cTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACCATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTg        <  2:192014/140‑1 (MQ=255)

                                                                                                                            tttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGAAGGTGATATTACTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTGTAAGTTTAAAAGCTTTAATTACTTTAACCATTGc       >  1:61046/1‑140 (MQ=14)

                                                                                                                             ttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCt      >  1:211349/1‑140 (MQ=255)

                                                                                                                                cTGTATTGATACTTTGATGCCTTGTCTTTCTTACGTTAATACGACGGTGATCTTGCTCAATGAGGTTATTCAGATATCTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc   <  1:22512/140‑1 (MQ=14)

                                                                                                                                 tGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATAGGACTGGGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt  <  2:33415/140‑1 (MQ=14)

AGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCT  >  CP000730/35868‑36136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`