Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 638,614 G→T 100% T14K (ACA→AAA)  thiD ← phosphomethylpyrimidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730638,6140GT100.0% 293.5 / ‑6.5 90T14K (ACA→AAA) thiDphosphomethylpyrimidine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (41/48);  total (41/49)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTGTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAAT  >  CP000730/638479‑638750
                                                                                                                                       |                                                                                                                                         
tCCATTGGTAATGGGGTAACATCGTGTGAACATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCa                                                                                                                                      <  2:533655/141‑1 (MQ=255)
  caatGGTAATGGTGTAACATCGTGTGACCATGTATCTTTTTCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGa                                                                                                                                    <  1:158031/138‑1 (MQ=255)
    ttGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAAc                                                                                                                                  <  1:658995/141‑1 (MQ=255)
     tGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAAcc                                                                                                                                 <  2:420561/141‑1 (MQ=255)
      ggTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCg                                                                                                                                <  2:438595/141‑1 (MQ=255)
      ggTAATGGTGTAACATCGTCTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCg                                                                                                                                <  2:499324/141‑1 (MQ=255)
          aTGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAACGCGACCCCTCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCaa                                                                                                                           <  1:564487/141‑1 (MQ=255)
          aTGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCaa                                                                                                                           <  1:488752/141‑1 (MQ=255)
              tgtAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGt                                                                                                                       >  1:272451/1‑141 (MQ=255)
                tAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTa                                                                                                                     <  1:654127/141‑1 (MQ=255)
                 aaCATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTaa                                                                                                                    <  1:269383/141‑1 (MQ=255)
                    aTCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAAc                                                                                                                 <  2:46559/141‑1 (MQ=255)
                     tCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAAct                                                                                                                >  2:659651/1‑141 (MQ=255)
                     tCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAAct                                                                                                                >  2:298000/1‑141 (MQ=255)
                        tgtgACAATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAActttc                                                                                                             <  1:325577/141‑1 (MQ=255)
                         gtgATCATGTAGCTTTATCCATGGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAActttct                                                                                                            <  1:204627/141‑1 (MQ=255)
                          tgACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAActttctt                                                                                                           <  1:675823/141‑1 (MQ=255)
                           gACCATGTATCTTTATCCATAGCAACGATGGCAGTTAAAGCGCCCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAActttcttt                                                                                                          <  1:66855/141‑1 (MQ=255)
                            acaaTGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTa                                                                                                         <  2:157446/138‑1 (MQ=255)
                              cATGTATCTTTATCCATAGTAACGATGGCCGTTCAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCCCTTTTGTCAGACCCGCAAATTGTTAAACCTTACTTTaaa                                                                                                       >  1:555020/1‑141 (MQ=255)
                                 gTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCATAACCGGCCATTGTTAAAACTTTCTTTAAAGcc                                                                                                    >  2:426680/1‑141 (MQ=255)
                                        tATCCATAGTAACGATGGCAGTTAAAGCGCCCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTCAAAGCCATTTAGc                                                                                             <  2:282589/141‑1 (MQ=255)
                                         aTCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCt                                                                                            <  2:496093/141‑1 (MQ=255)
                                          tcgaTAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCtt                                                                                           <  1:706429/138‑1 (MQ=255)
                                          tCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGGAATTGTTAAAACTTTCTTTAAAGCCATTGAGCtt                                                                                           >  2:73753/1‑141 (MQ=255)
                                          tCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCtt                                                                                           >  1:752517/1‑141 (MQ=255)
                                            cATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCa                                                                                         <  1:718860/141‑1 (MQ=255)
                                            cATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCa                                                                                         >  1:676649/1‑141 (MQ=255)
                                                gTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTcc                                                                                     <  1:409509/141‑1 (MQ=255)
                                                 tAACGCTTGCAGTTAAAGCGAGCATGCCCTACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCt                                                                                    <  1:95523/141‑1 (MQ=255)
                                                 tAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCt                                                                                    <  2:522747/141‑1 (MQ=255)
                                                    cGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATATGCTTGCATACCTGCGCCAGCACTTTTATCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGATTCACTCCTACa                                                                                 >  1:90993/1‑141 (MQ=255)
                                                     gATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACat                                                                                <  2:2521/141‑1 (MQ=255)
                                                     gATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACat                                                                                <  1:622262/141‑1 (MQ=255)
                                                      aTGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACata                                                                               >  2:671706/1‑141 (MQ=255)
                                                      aTGGCAGTTAAAGCGACCATGCCATACGTATCTAATCCTTGGACCGCTTTAAAATCTGCTTGCATAAATGAGCCAGCAATATTGTGAGAACCGGCAAACTGTTAAACCTTCTTTAAAGCCTTTGAGCTTCACTCCTACata                                                                               >  2:449420/1‑141 (MQ=255)
                                                        ggCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataat                                                                             >  2:276989/1‑141 (MQ=255)
                                                          cAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTGAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataataa                                                                           >  1:42393/1‑141 (MQ=255)
                                                           atttAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataataat                                                                          <  2:539618/139‑1 (MQ=255)
                                                              tAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACGCCTACATAATAATAtt                                                                       >  1:135910/1‑141 (MQ=255)
                                                              aaaaGCGACCATGCCATACGTAGCTAATGCTAGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATAtt                                                                       >  2:771100/2‑141 (MQ=255)
                                                               aacgCGACCACGCCATACGTATCTAATTTTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTg                                                                      <  2:203865/138‑1 (MQ=255)
                                                               aaaGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGACCCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTg                                                                      <  1:107423/141‑1 (MQ=255)
                                                                aaGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTTCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGACATTGAGCTTCACTCCTACATAATAATAtttt                                                                     >  2:372536/1‑139 (MQ=255)
                                                                aaGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGt                                                                     >  1:740766/1‑141 (MQ=255)
                                                                aaGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGGTAAAACTTTCTTTAAAGCCCTTGAGCTTCACTCCTCCCTAATAATATTGt                                                                     >  2:372477/1‑141 (MQ=255)
                                                                   cGACCATGCCATACGTATCTAATTCTGGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTAtt                                                                  <  1:15231/141‑1 (MQ=255)
                                                                      ccATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTGTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATtcat                                                               >  1:779583/1‑141 (MQ=255)
                                                                        ctGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATtcatca                                                             >  2:283053/2‑141 (MQ=255)
                                                                        aTGCCATACGTAGCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATtcatca                                                             >  2:283055/1‑141 (MQ=255)
                                                                          gCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATACTAATATTGTATTcatcatt                                                           >  1:576058/1‑140 (MQ=255)
                                                                           ccgtactgaTCTAATTCTGTGAAGGGTGTCACATCTGCTGGCATACCTGGGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAACCTTTCTTTAAAGTCCGTTAGCTTCACTCCTTCCTTATAATATTGTATtcattctat                                                          <  2:722826/133‑6 (MQ=255)
                                                                            cATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCatcatatc                                                         <  1:641124/141‑1 (MQ=255)
                                                                              tACGTATCTAAATCTTGGAACGTTTTCAAGTCCGCGTGGATACCTGCGCCAGTCCTTTTGTCAGAATCGGCAATTGTTAAAAAGTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCatcatatcat                                                       <  2:210145/141‑1 (MQ=255)
                                                                              tACCTATCCAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCatcatatcat                                                       <  1:357652/141‑1 (MQ=255)
                                                                               aCGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAtt                                                      <  2:272451/141‑1 (MQ=255)
                                                                               aCGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAtt                                                      >  1:7131/1‑141 (MQ=255)
                                                                               aCGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAtt                                                      >  2:405895/1‑141 (MQ=255)
                                                                                cGTATCTAATTCTTGGGACGTTTTCAAATCTGCTTGCATCCCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAACACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAttt                                                     <  2:112929/141‑1 (MQ=255)
                                                                                cGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTTCATACCTGCGCCAGCACTTTTGTCATAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATGGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAttt                                                     >  1:67153/1‑141 (MQ=255)
                                                                                cGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAttt                                                     <  1:670064/141‑1 (MQ=255)
                                                                                 gTATCTAATTCTTGTAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAtttt                                                    <  2:343295/141‑1 (MQ=255)
                                                                                  tATCTTATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAttttt                                                   <  2:515689/141‑1 (MQ=255)
                                                                                  tATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCAttttt                                                   <  2:134005/141‑1 (MQ=255)
                                                                                   aTCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTa                                                  >  2:724826/1‑141 (MQ=255)
                                                                                   aTCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATAAtttttt                                                  >  2:540752/1‑140 (MQ=255)
                                                                                    tCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTaa                                                 >  2:43610/1‑141 (MQ=255)
                                                                                      tAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAAcc                                               <  1:714602/141‑1 (MQ=255)
                                                                                           cTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCATTTGTTAACACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTCAtt                                          >  2:287517/1‑141 (MQ=255)
                                                                                           cTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAAtt                                          <  1:276989/141‑1 (MQ=255)
                                                                                                aaCGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTGATTGaaaa                                     <  2:265551/141‑1 (MQ=255)
                                                                                                aaCGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTAATCATATCATTTTTAACCTAATTGaaaa                                     >  2:772108/1‑141 (MQ=255)
                                                                                                   gTTTTCGAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAata                                  >  2:650506/1‑141 (MQ=255)
                                                                                                   gTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTTTCAGAACCGGCTATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATGATATTTAACCTAATTGATCAata                                  >  1:138325/1‑141 (MQ=255)
                                                                                                   gTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCACTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATAATTTTTAACCTAATTGAAAtata                                  >  2:561193/1‑141 (MQ=255)
                                                                                                     tttCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATCTCATTTTTAACCTAATTGAAAAATAtt                                >  1:217172/1‑141 (MQ=255)
                                                                                                       tCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTaa                              >  2:342489/1‑141 (MQ=255)
                                                                                                          aaTCTGCTTGCATACCTGCCCCAGCACTTTTTTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCa                           <  2:604069/141‑1 (MQ=255)
                                                                                                            tCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCAtt                         >  2:593796/1‑141 (MQ=255)
                                                                                                               gCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATACTAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAATTATTAAGCATTcgt                      >  2:340422/1‑139 (MQ=255)
                                                                                                                       aCCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAATCTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTtgat              <  1:125226/141‑1 (MQ=255)
                                                                                                                            cgcCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGAttg         >  1:299668/1‑141 (MQ=255)
                                                                                                                             gcCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTCGATGAttgt        <  2:499435/141‑1 (MQ=255)
                                                                                                                               cAGCACTTTTGTCATACCCGTCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATATTATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGAttgttg      <  2:756163/141‑1 (MQ=255)
                                                                                                                               cAGCACTTTTGTCAGTACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGAttgttg      <  2:244493/141‑1 (MQ=255)
                                                                                                                                aGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCAATCCTACATAATAATATTGTTTTCTTCTTATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGa     >  1:609203/1‑141 (MQ=255)
                                                                                                                                  cACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGaaa   >  1:593145/1‑141 (MQ=255)
                                                                                                                                  cACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATAATTCTTaaaa   >  2:623900/1‑141 (MQ=255)
                                                                                                                                  cACTTGTGTCAGAACCGGCAATTGGTAAAACTTGCTTTAACGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAAACTAAATGAAAAATATTAAGCATTCAATATTTGATGATTGTTGaaa   <  2:55007/141‑1 (MQ=255)
                                                                                                                                   aCTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAAt  <  2:40812/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                         
TCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAATTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTGTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGATTGTTGAAAT  >  CP000730/638479‑638750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: