Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,232,465 G→C 100% A124P (GCG→CCG)  USA300HOU_1157 → non‑specific serine/threonine protein kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,232,4650GC100.0% 286.8 / ‑6.5 85A124P (GCG→CCG) USA300HOU_1157non‑specific serine/threonine protein kinase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (41/43);  total (41/44)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGAAGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATC  >  CP000730/1232329‑1232597
                                                                                                                                        |                                                                                                                                    
agaagaTGCCTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCa                                                                                                                                  <  1:347243/141‑1 (MQ=255)
agaagaTGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCa                                                                                                                                  <  1:466712/141‑1 (MQ=255)
agaagaTGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCa                                                                                                                                  <  1:50263/141‑1 (MQ=255)
       gACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATg                                                                                                                           >  2:494350/1‑141 (MQ=255)
       gACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATg                                                                                                                           >  1:461191/1‑141 (MQ=255)
        ccTGTTACTACTTAGTAAGGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGc                                                                                                                          <  2:586503/140‑1 (MQ=255)
        attGTTATTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACCTCCGCATGATATGc                                                                                                                          <  1:403634/139‑1 (MQ=255)
         cTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCg                                                                                                                         >  1:232364/1‑141 (MQ=255)
         cTGTTACTACTTAGTAATGGAATATAACGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCg                                                                                                                         <  1:1865/141‑1 (MQ=255)
          tGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGACTCAAATATTGGATGGCCTTAAACATCCGCATGataagagg                                                                                                                        >  2:380315/1‑136 (MQ=255)
           gTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCCTTAAGTGTTGACCCGGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGAAATGCGTa                                                                                                                       >  2:64285/1‑141 (MQ=255)
           gTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTa                                                                                                                       >  1:308200/1‑141 (MQ=255)
           gTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTa                                                                                                                       <  1:631318/141‑1 (MQ=255)
           gTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATACgga                                                                                                                       >  2:268923/1‑139 (MQ=255)
                     aGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGCATCAAATATTGGATGGCATTGACAATCCGCATGATATGCGTGTTGTTCATag                                                                                                             >  2:413623/1‑141 (MQ=255)
                       caaTGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATagag                                                                                                           <  2:341809/140‑1 (MQ=255)
                        aaTGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATagagt                                                                                                          >  1:132610/1‑140 (MQ=255)
                        aaTGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAAGTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAgaga                                                                                                          >  2:233333/1‑141 (MQ=255)
                         aTGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGat                                                                                                         <  2:98759/141‑1 (MQ=255)
                         aTGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGat                                                                                                         <  2:508523/141‑1 (MQ=255)
                          tGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGata                                                                                                        >  2:559544/1‑141 (MQ=255)
                           ggAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAACGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCCTGATATGCGTATTGTACATagcgtgat                                                                                                       >  2:774609/1‑135 (MQ=255)
                               tatatCGAATGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGAAACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAg                                                                                                   <  1:67294/141‑1 (MQ=255)
                                atatCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGc                                                                                                  >  2:436764/1‑141 (MQ=255)
                                 atgcGAGGGTCCGCCTTTGTCTGAGGATATTGAAAGTTATGGGTCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGTATGGCATTAAACAGCCGCATGATATGCGTATTGTACATAGAGATATTAAGcc                                                                                                 <  2:560411/138‑1 (MQ=255)
                                   tCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCcac                                                                                               <  2:197245/141‑1 (MQ=255)
                                        ggcccGATTTTGTTTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGTATGGCATTAAACAGCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAat                                                                                          <  2:345931/138‑1 (MQ=255)
                                           ccGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatata                                                                                       >  1:461407/1‑141 (MQ=255)
                                           ccGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatata                                                                                       >  2:341891/1‑141 (MQ=255)
                                           ccGACGTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatata                                                                                       <  1:631794/141‑1 (MQ=255)
                                            cGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatatat                                                                                      >  1:79438/1‑141 (MQ=255)
                                            cGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatatat                                                                                      >  1:274874/1‑141 (MQ=255)
                                             gACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATAtt                                                                                     >  1:669330/1‑141 (MQ=255)
                                                  tGTCTGAGTATATTGAAAGTCATGGGCCATTAAGGGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCGTGATATGCGTATTGTACATAGCGATATTAAGCCACAAAATATATTAATTg                                                                                >  1:449685/1‑141 (MQ=255)
                                                           ctatGGAAAGTCAGTGGCCATTAAGTGTTGACACAGTGATTAATTGTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATa                                                                       <  2:711788/140‑1 (MQ=255)
                                                            tatTGAAAGTCCTGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATaa                                                                      <  2:641444/141‑1 (MQ=255)
                                                             atTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATaaa                                                                     <  2:184002/141‑1 (MQ=255)
                                                              ttGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATaaaa                                                                    >  2:756687/1‑141 (MQ=255)
                                                                  aaGTCATGGGTCATTAAGTGTTGACACAGCGAGTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGtt                                                                <  2:669902/141‑1 (MQ=255)
                                                                     tCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCCATAAAACGTTGaa                                                             >  1:462080/1‑141 (MQ=255)
                                                                      cATGGGCCATTAAGTGTTGACACAGCGATTCATTTTACGAATCAAATATTGGCTGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGaaa                                                            >  2:29288/1‑141 (MQ=255)
                                                                       aTGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGaaaa                                                           >  2:398931/1‑141 (MQ=255)
                                                                        tGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGaaaaa                                                          >  2:455417/1‑140 (MQ=255)
                                                                          ggCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAatat                                                        >  1:337052/1‑141 (MQ=255)
                                                                          ggCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAatat                                                        >  1:729778/1‑141 (MQ=255)
                                                                           gCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATAtt                                                       <  1:624430/141‑1 (MQ=255)
                                                                           gCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATAtt                                                       <  2:311323/141‑1 (MQ=255)
                                                                            ccATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATAttt                                                      >  2:703649/1‑141 (MQ=255)
                                                                            ccATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATAttt                                                      >  2:178588/1‑141 (MQ=255)
                                                                             cATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAGAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTg                                                     <  1:553400/141‑1 (MQ=255)
                                                                             cATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTg                                                     <  2:377530/141‑1 (MQ=255)
                                                                               atAAGTTTTAACACAGCGAATAATTTTAAGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGAt                                                   <  1:654554/140‑1 (MQ=255)
                                                                                     gtTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGa                                             <  1:427804/141‑1 (MQ=255)
                                                                                       tGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAAt                                           <  2:709384/141‑1 (MQ=255)
                                                                                         acacAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTg                                         <  2:89505/141‑1 (MQ=255)
                                                                                          cacaGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGGGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGc                                        >  1:86373/1‑141 (MQ=255)
                                                                                          aacaGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGc                                        <  1:164910/140‑1 (MQ=255)
                                                                                           acaGCGATTAATTTGACGACTCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGACTTTGGAATTGCt                                       >  1:426290/1‑141 (MQ=255)
                                                                                            caGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTa                                      >  1:633337/1‑141 (MQ=255)
                                                                                            caGCGAGTAATTTGACGAATCAAATATTGGATGGCATTAAACATCCGCATTGTATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAAGTGACAGCAATAAAACGGTGAAAATATTTGATTTTGGAATTGCTa                                      <  2:210750/141‑1 (MQ=255)
                                                                                            aaGCGATTAATTTTACGAATAAAATATTGGATAGCATTAAACAACCGTATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTa                                      <  1:130879/140‑1 (MQ=255)
                                                                                                 aTTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAGTATTTGATTTTGGAATTGCTAAAGCt                                 >  1:480290/1‑141 (MQ=255)
                                                                                                  ttAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCtt                                >  2:578330/1‑141 (MQ=255)
                                                                                                  ttAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATAGTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGGATTGCTAAAGCtt                                >  1:4768/1‑141 (MQ=255)
                                                                                                    aaTTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACCTAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGCACATCTTTAATTTTGGAATTGCTAAAGCTTTa                              >  2:400473/1‑141 (MQ=255)
                                                                                                    aaTTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAACCTTTa                              >  1:110461/1‑141 (MQ=255)
                                                                                                         taagAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTga                         <  1:608724/138‑1 (MQ=255)
                                                                                                          aCGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTgag                        >  1:744223/1‑141 (MQ=255)
                                                                                                           cGAATTAAATCTTTGATGGCATTAAACATCCGCATGATATGCTTATTGTACATAGAGATATTAAGCCAGAAAATATATTAACTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAACTGCTAAAGCTTTAAGTgaga                       <  2:581290/141‑1 (MQ=255)
                                                                                                               tCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAGACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTc                   >  1:510758/1‑141 (MQ=255)
                                                                                                               tCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTc                   >  1:756085/1‑141 (MQ=255)
                                                                                                                 aaaTATTGGAAGGCATTAAACAACCGCATGATATGCGTATTGAACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCtt                 <  1:520353/141‑1 (MQ=255)
                                                                                                                  aaTATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCttt                <  2:312161/141‑1 (MQ=255)
                                                                                                                    tatTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTaa              <  2:461191/141‑1 (MQ=255)
                                                                                                                    tatTCGATGGCATTATACCTCCAAATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAAGGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTaa              <  1:521263/141‑1 (MQ=255)
                                                                                                                     atTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAAc             >  1:725911/1‑141 (MQ=255)
                                                                                                                       tGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTc           <  2:337052/141‑1 (MQ=255)
                                                                                                                       tGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTc           <  2:729778/141‑1 (MQ=255)
                                                                                                                         gATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGGTAAAGCTTTAAGTGAGACGTCTTTAACTCAg         <  1:373838/141‑1 (MQ=255)
                                                                                                                         gATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAg         >  1:1730/1‑141 (MQ=255)
                                                                                                                          aTGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGa        <  2:30835/141‑1 (MQ=255)
                                                                                                                          aTGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGa        <  1:703771/141‑1 (MQ=255)
                                                                                                                                ttAAACATGCGAATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATc  <  1:720626/141‑1 (MQ=255)
                                                                                                                                ttAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATc  <  1:25223/141‑1 (MQ=255)
                                                                                                                                ttAAACATCCGCATGAGATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATc  <  2:153706/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
AGAAGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATC  >  CP000730/1232329‑1232597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: