Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,476,694 T→C 5.1% N8444D (AAC→GAC)  ebh ← extracellular matrix binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,476,6940TC 5.1% 240.7 / 2.8 79N8444D (AAC→GAC) ebhextracellular matrix binding protein
Reads supporting (aligned to +/- strand):  ref base T (39/36);  new base C (2/2);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

TTCATTGATAATATTATTTGCGGCTTGAACAGCTTGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGCATCAATTGCTTGCGTCGCATTTGTTTTAG  >  CP000730/1476554‑1476831
                                                                                                                                            |                                                                                                                                         
ttCATTGATAATATTATTTGCGGCTTGAACAGCTTGTTCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGAGCTATTTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGtt                                                                                                                                           <  2:128325/141‑1 (MQ=255)
    ttGATAATATTATTTGCGGCTTGAACAGCTTGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGc                                                                                                                                       <  2:763899/141‑1 (MQ=255)
        taataTTTTTTGCGGCTTGAACAGCTTGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTAt                                                                                                                                   <  1:136329/141‑1 (MQ=255)
        taataTTATTTGAGGCTTGAACAGCTTGATCATAGTTCTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTGTCTTGAATGCTCTGTCTTAAACCATGCCTTGCTTGGGTAACCGAAt                                                                                                                                   >  2:693092/1‑141 (MQ=255)
         actcacatgtGCGGCGTGAACAGCTTAATCATCGTTTTGTTCCGCTTGTTGATCTTCTTTGATATATTTGCCATTTGCTTTAGTTGCTGCGTTATCTTGAATTCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTAtt                                                                                                                                  <  1:62445/132‑1 (MQ=255)
         aataTTATTTGCGGCTTGAACAGCTTGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTAtt                                                                                                                                  >  1:752455/1‑141 (MQ=255)
         aataTTATTTGCGGCTTGAACAGCTTGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTAtt                                                                                                                                  >  1:47193/1‑141 (MQ=255)
          atatTATTTGCGGCTTGAACAGCTTGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTCTTc                                                                                                                                 >  2:199777/1‑141 (MQ=255)
            attattTGCGGCTTGAACAGCTTGATCATAGTTTTGTTGATCTGATTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTTGTTAAGTGTATTCGc                                                                                                                               >  2:509421/1‑141 (MQ=255)
                 ttGCGGCTTGAACAGCTTGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCAtttt                                                                                                                          >  2:714551/1‑141 (MQ=255)
                 ttGCGGCTTGAACAGCTTGATCATAGTGTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTGGAATGCTCTGTCTTAAACCATGCATTGTTTGCTTAAGCGGAATCGCAtttt                                                                                                                          >  2:77572/1‑141 (MQ=255)
                     ggCTTGAACAGCTTGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTGTTCGCATTTTGTTc                                                                                                                      >  2:196505/1‑141 (MQ=255)
                     ggCTTGAACAGCTTGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTc                                                                                                                      >  1:710945/1‑141 (MQ=255)
                        ttGAACAGCTTGTTCATATTTTTGTTGCTCTGGTTTACCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAAt                                                                                                                   <  1:211043/141‑1 (MQ=255)
                          gAACAGCTTGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAAttt                                                                                                                 >  2:199366/1‑141 (MQ=255)
                            aCAGCTTGTTCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTtga                                                                                                               <  1:15928/141‑1 (MQ=255)
                            aCAGCTTGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATTTATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGAATTGCTCGGTTAGGCGTGTTCGCATTTTGTTCAATTtga                                                                                                               >  2:366629/1‑141 (MQ=255)
                            aCAGCTTGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTtga                                                                                                               >  1:630765/1‑141 (MQ=255)
                            aCAGCTTGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTtga                                                                                                               >  2:486964/1‑141 (MQ=255)
                            aCAGCTTGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTCAAGCGTATTCGCATTTTGTTCAATTtga                                                                                                               >  2:487159/1‑141 (MQ=255)
                            aCAGCTTGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTCAAGCGTATTCGCATTTTGTTCAATTtga                                                                                                               >  2:487161/1‑141 (MQ=255)
                              aGCTTGAACAAAGTTTTGTTGCTCTGGTTGATCTTCGTTGATAAATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTCAACGTGTTCGCTTTTTGTTCAAtttacta                                                                                                             >  2:678550/1‑137 (MQ=255)
                              aGCTCGATCATAGTTTTGGTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTCGTTTCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTtgatg                                                                                                             <  1:764088/141‑1 (MQ=255)
                                 ttgcttaTAGTTTTGTTGCTCTGGTTGATCTTCGTCGATATCTTTGCTTTTTTCTTGAGTTGCTCCCTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCTCATTTTGTTCAATTTGATGAAc                                                                                                          <  1:601614/135‑1 (MQ=255)
                                 ttGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAAc                                                                                                          <  1:632344/141‑1 (MQ=255)
                                  tGAGCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACt                                                                                                         <  1:219569/141‑1 (MQ=255)
                                        tAGTTTTGTTGCTCTTGTTTATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGtt                                                                                                   <  1:417964/141‑1 (MQ=255)
                                         aGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTa                                                                                                  <  1:233361/141‑1 (MQ=255)
                                          gTTTTGTTGCTCGGGTTGATCTTCGTTGATAGATTTGCTATTGGCTTTAGTTGCTGCGGTATCGTGAATGCTCTGTCTTGAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTAc                                                                                                 <  1:664998/141‑1 (MQ=255)
                                             ttgttgTTCTGGTTGTCCTTCGTTGATATATTTGCTATTTGCTTTTGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTaa                                                                                              <  2:289989/141‑1 (MQ=255)
                                               gttgCTCGGGTTGATCTTCGTTGACATCGTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAg                                                                                            >  2:749354/1‑141 (MQ=255)
                                                  gCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGTACTGCAGTTACTAAAGTTg                                                                                         >  2:168997/1‑141 (MQ=255)
                                                   ctctGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGc                                                                                        >  1:278185/1‑141 (MQ=255)
                                                      tGGTTTATCTTCGTTGATATATTTGCTATTCGCTTTAGTCGCTGCGCTATCTTGAATGCTCAGTCTTAAACCATACATTTCTTGGTTAAGCGTATTCGCATTTCGTTCAATTTGATGAACTGCAGTTACTAAAgaaccagc                                                                                     <  2:118086/141‑8 (MQ=255)
                                                      tGGTTGATCTTCGTTGATACCAGTTCGATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGc                                                                                     >  2:551340/1‑141 (MQ=255)
                                                       ggTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGCCTCAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCt                                                                                    <  1:179742/141‑1 (MQ=255)
                                                         ttGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGTTCTGGCCTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGt                                                                                  <  1:564192/141‑1 (MQ=255)
                                                         ttGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGt                                                                                  >  1:635987/1‑141 (MQ=255)
                                                          tGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTCAATGCACTGTCATAAACGATTCACTGCTCGGTTAAGCGTATTCGCTTTTGGTTCAATTGCATGAACTGCAGCTCCTGAAGTCGCGGCTGTa                                                                                 >  2:124825/1‑141 (MQ=255)
                                                          tGATCTTCGTTGATATATTTCCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAAATGCAGTTACTAAAGTTGCAGATGTa                                                                                 >  1:467031/1‑141 (MQ=255)
                                                            aTCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTGATCTTGAATGATTTATAATTAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGGTACTAAAGTTGCAGCTGTGAc                                                                               >  1:529178/1‑141 (MQ=255)
                                                             tCTTCGTTGATATATATGCTTTATGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACt                                                                              >  2:751038/1‑141 (MQ=255)
                                                             tCTTCGTTGATATAGTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGTTGAACTGCAGTTACTAAAGTTGCAGCTGTAACt                                                                              <  2:471072/141‑1 (MQ=255)
                                                               ttCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTg                                                                            <  2:647184/141‑1 (MQ=255)
                                                                      atatatTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCtttt                                                                     <  2:668532/141‑1 (MQ=255)
                                                                       tatatTTGCTATTTGCTTTCGTAGCGGCGGTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGCACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTa                                                                    >  2:356503/1‑141 (MQ=255)
                                                                            ttgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTTTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCt                                                               <  2:210926/141‑1 (MQ=255)
                                                                            ttgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCt                                                               <  1:451939/141‑1 (MQ=255)
                                                                                 ttttgctTTAGTTGCTGCGTTATCTTGAATGGTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGtttt                                                          <  2:457145/140‑1 (MQ=255)
                                                                                    tgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTg                                                       >  2:735546/1‑141 (MQ=255)
                                                                                     gctTTAGTTGCTGCGCTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTgg                                                      >  1:574844/1‑140 (MQ=255)
                                                                                      ctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTAGATGACCTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGATGTTTTTTGAg                                                     >  2:196048/1‑141 (MQ=255)
                                                                                       tttAGTTGCTGCGTTATCTTGAATGCTCTGTTTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAgg                                                    <  1:216988/141‑1 (MQ=255)
                                                                                           gTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCa                                                >  1:199775/1‑141 (MQ=255)
                                                                                               ctgcGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTTGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTa                                            <  2:685573/141‑1 (MQ=255)
                                                                                                tgcGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTaa                                           <  1:750725/141‑1 (MQ=255)
                                                                                                 tcgtgaggttGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAg                                          <  2:777303/133‑1 (MQ=255)
                                                                                                  cGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGc                                         >  1:159155/1‑141 (MQ=255)
                                                                                                     tATCTTGAATGCTCTTTCTTAAACCATGCATTGCTTGGTTAAGCTTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTg                                      <  2:4988/141‑1 (MQ=255)
                                                                                                     tATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTg                                      <  2:298994/141‑1 (MQ=255)
                                                                                                       tCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGtt                                    >  2:715978/1‑141 (MQ=255)
                                                                                                           gAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTACGCtagctcaag                                >  2:563009/1‑133 (MQ=255)
                                                                                                           gAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAg                                >  1:322605/1‑141 (MQ=255)
                                                                                                           gAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAg                                <  1:542298/141‑1 (MQ=255)
                                                                                                            aaTGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGc                               >  1:171276/1‑141 (MQ=255)
                                                                                                                ctctGCCTTAATCCCTGCATTGCTTGTTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGCATCa                           <  2:463089/141‑1 (MQ=255)
                                                                                                                       ttAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGCATCAAttgctt                    <  2:777527/141‑1 (MQ=255)
                                                                                                                         aaaCCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGCATCAAttgcttgc                  <  1:618612/141‑1 (MQ=255)
                                                                                                                             cATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGCATCAATTGCTTGCGTCg              <  2:514953/141‑1 (MQ=255)
                                                                                                                              aTGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGCATCAATTGCTTGCGTCGc             <  2:630765/141‑1 (MQ=255)
                                                                                                                              aTGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGCATCAATTGCTTGCGTCGc             <  1:486964/141‑1 (MQ=255)
                                                                                                                              aTGCATTGCTTGGTCAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGCATCAATTGCTTGCGTCGc             <  1:487161/141‑1 (MQ=255)
                                                                                                                              aTGCATTGCTTGGTCAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGCATCAATTGCTTGCGTCGc             <  1:487159/141‑1 (MQ=255)
                                                                                                                                gCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGCATCAATTGCTTGCGTCGCAt           >  1:414401/1‑141 (MQ=255)
                                                                                                                                  aTTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGCATCAATTGCTTCCGTCACAttt         >  2:563549/1‑141 (MQ=255)
                                                                                                                                   ttGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGCATCAATTGCTTGCGTCGCATTTg        >  1:677426/1‑141 (MQ=255)
                                                                                                                                        tGGTTAAGGGGTTTCGCCTTTTGCTACATGTGATGAACTGCATTTACTAAAGTTGCAGCTGGAACTTGGTCTCTTAATGCTCTTTTTCGAGGTTCATTTAAGCTTGTTAAAGCATCAATTGCTTGCGTCGCATTTGTTTTa   <  2:273700/141‑1 (MQ=255)
                                                                                                                                         ggTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGCATCAATTGCTTGCGTCGCATTTGttttgg  >  1:94271/1‑139 (MQ=255)
                                                                                                                                         ggTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGCATCAATTGCTTGCGTCGCATTTGTTTTAg  >  1:656745/1‑141 (MQ=255)
                                                                                                                                         ggTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTGGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGCATCAATTGCTTGCGGCGCATTTGTTTTAg  >  2:591009/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                         
TTCATTGATAATATTATTTGCGGCTTGAACAGCTTGATCATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTTTTTGAGGATCATTTAAGCTTGTTAAAGCATCAATTGCTTGCGTCGCATTTGTTTTAG  >  CP000730/1476554‑1476831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: