Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,822,730 T→A 5.5% intergenic (+51/+195) USA300HOU_1697 → / ← ackA possible universal stress protein/acetate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,822,7300TA 5.5% 193.8 / 2.5 66intergenic (+51/+195)USA300HOU_1697/ackApossible universal stress protein/acetate kinase
Reads supporting (aligned to +/- strand):  ref base T (32/29);  new base A (2/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

GTGTTAGTTGTTCGTACTGAAGAGTTACCAGCAGACTTCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGAAAAGAGACATTACACTAGTTAATTTTCAAACAATACAAAA  >  CP000730/1822593‑1822865
                                                                                                                                          |                                                                                                                                       
gtgtTAGTTGTTCGTACTGAAGAGTTACCAGCAGACTTCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTc                                                                                                                                      <  2:673696/141‑1 (MQ=255)
  gtTAGTTGTTCGTACTGAAGAGTTACCAGCAGACTTCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTg                                                                                                                                    <  2:112224/141‑1 (MQ=255)
    tAGTTGTTCGTACTGAAGAGTTACCAGCAGACTTCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCa                                                                                                                                  >  2:44162/1‑141 (MQ=255)
    tAGTTGGTCGTACTGAAGAGGTACCAGCCGACTTCCAACCACCAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCa                                                                                                                                  <  1:342585/141‑1 (MQ=255)
      gttgttCGTACGGAAGAGTTACCAGCAGACTTCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACt                                                                                                                                <  1:169849/141‑1 (MQ=255)
       ttgttCGTACTGAAGAGTTGCCGGCAGACTTCCAACCACTAGTTGCAACAACTCAGTTAGGTGAAAAATATCAGAATTATATATAGATGATCACTAGATAACTACTTGATCATTATGTACCGAGTGCA‑ACTTTCTGCAAtt                                                                                                                               >  1:655227/1‑141 (MQ=255)
          ttCGGACTGAAGAGTTACCAGCAGACTTCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAAAAGAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGt                                                                                                                            <  1:107149/141‑1 (MQ=255)
            cGTACTGAAGAGTTACCAGCAGACTTCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGttt                                                                                                                          <  2:651159/141‑1 (MQ=255)
            cGTACCGAAGAGTTACCAGCAGACTTCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGttt                                                                                                                          <  1:220743/141‑1 (MQ=255)
             gTACTGAAGAGTTACCAGCAGACTTCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGTTCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGtttt                                                                                                                         <  1:381706/141‑1 (MQ=255)
               aCTGAAGAGTTACCAGCAGACTTCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGtttttt                                                                                                                       >  2:543126/1‑141 (MQ=255)
               aCTGAAGAGTTACCAGCAGAATTATATCCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGtttttt                                                                                                                       >  1:374748/1‑141 (MQ=255)
                cTGAAGACGTAACAGCAGACTTCCAACCAGAAGTTGCAAAAACTCAATTACTTGAAAAATATCAAAATTAAATATAGATGATCATTATATAACTACTTGATCATTATGAACCAAGTGCA‑ACGTTCTGCACTTGGTTTTTTa                                                                                                                      <  2:167992/141‑1 (MQ=255)
                  gAAGAGTTACCAGCAGACTTCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTAtt                                                                                                                    >  2:425537/1‑141 (MQ=255)
                    agagTTACCAGCAGACTTCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGt                                                                                                                  <  2:712437/141‑1 (MQ=255)
                       gTTACCAGCAGACTTCCAACCACAAGTTGCAACAACTCCATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTta                                                                                                               <  1:775237/141‑1 (MQ=255)
                          acaagcagACTTCCAACCACAAGTTGCAACAACTCAATTAGGTGAAAAATATCAAAATTCAATTTAGATGATCATTAGATAACTACTTGGTCATTGTAAACCAAGTGAA‑ACTTTGTGAACTTGGTTTTTTCTTGttattaa                                                                                                            >  2:221243/4‑136 (MQ=255)
                                 gACTTCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAAc                                                                                                     >  2:568942/1‑141 (MQ=255)
                                 gACTTCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAAc                                                                                                     <  1:404620/141‑1 (MQ=255)
                                   cTTCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTc                                                                                                   >  1:432489/1‑141 (MQ=255)
                                     tCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCAc                                                                                                 <  1:156240/141‑1 (MQ=255)
                                       cAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAATATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACtt                                                                                               <  2:243886/141‑1 (MQ=255)
                                        aaCCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTta                                                                                              <  1:610606/141‑1 (MQ=255)
                                            acaAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGTTCAATTGGTAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTtaataa                                                                                          <  1:367401/141‑1 (MQ=255)
                                             caAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATCACTACTTGATCATTATGAACCAAGTGCA‑ACATTCTGCACTTGGTCTTTAATTGTTTTTAAATAAAACTCACTTaataat                                                                                         >  2:537874/1‑141 (MQ=255)
                                             caAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTaataat                                                                                         >  2:185210/1‑141 (MQ=255)
                                                gTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGtt                                                                                      >  1:408321/1‑141 (MQ=255)
                                                gTTGCAACAACTCAATTACGTGAAAAAAGTCCAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTATTAATGtt                                                                                      >  2:749010/1‑141 (MQ=255)
                                                 ttGCAACAACTCAATTACGTGCAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATACTTATGAACCAAGTGCA‑ACTTTGTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGttt                                                                                     >  2:369166/1‑141 (MQ=255)
                                                    caacaaCTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTCTTGTTTATAAATCAAACTCACTTAATAATGTTTTct                                                                                  >  1:521236/1‑140 (MQ=255)
                                                        aaagaattaagtagtcgaagaagattatgaaaacattattaagtgagttttatttataaacaataaaaAACCAAGTGCA‑GAAAGTTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAAt                                                                              <  1:736922‑M1/73‑1 (MQ=255)
                                                             aattagcttaaaaaTATCATAATTAAATACAGATGTTCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCGTCACTTGGTTTTTTATTGGTTATAAATAGAACTCACTTACTAATGTTTTCATAAtcttct                                                                         <  2:121159/132‑1 (MQ=255)
                                                              aTTACTTGAAAAATATCAAAATTAAAGATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATcttctt                                                                        <  2:744419/141‑1 (MQ=255)
                                                              aTTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATcttctt                                                                        >  1:747415/1‑141 (MQ=255)
                                                              aTTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATcttctt                                                                        >  1:461198/1‑141 (MQ=255)
                                                              aTTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACAAGAACATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACGTAATAATGTTTTCATAATcttctt                                                                        >  1:448778/1‑141 (MQ=255)
                                                               ttACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCttcttc                                                                       <  2:431383/141‑1 (MQ=255)
                                                                tACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATcttcttgg                                                                      >  1:398307/1‑139 (MQ=255)
                                                                   gTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGact                                                                   <  1:255079/141‑1 (MQ=255)
                                                                   gTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGact                                                                   <  1:674734/141‑1 (MQ=255)
                                                                    tGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGacta                                                                  <  1:486234/141‑1 (MQ=255)
                                                                        aaaTATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTa                                                              >  2:765978/1‑141 (MQ=255)
                                                                            atCAAAATTAAATATAGATGATCATTAGGTAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTc                                                          >  1:414947/1‑141 (MQ=255)
                                                                             tCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCt                                                         >  2:525622/1‑141 (MQ=255)
                                                                                            gatgatCATTAAATAACTACATGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGa                                          <  2:375306/141‑1 (MQ=255)
                                                                                              tgatCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGaaa                                        >  2:519816/1‑141 (MQ=255)
                                                                                               tatCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGaaaa                                       <  1:505111/140‑1 (MQ=255)
                                                                                                   aTTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTACTTCTTTAAGATATTCGTGAAAAaaga                                   >  2:459742/1‑141 (MQ=255)
                                                                                                    ttAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAGTTCTTTAAGATATTCGTGAAAAGAGAc                                  >  1:3820/1‑141 (MQ=255)
                                                                                                    gagttttatttataaacaataaaaAACCAAGTGCA‑GAAAGTTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTctctaagatattcctgtcacgtgct                                  <  2:660165‑M1/117‑13 (MQ=255)
                                                                                                     tAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGAAAAGAGACa                                 >  1:508334/1‑141 (MQ=255)
                                                                                                     tAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGAAAAGAGACa                                 >  2:623822/1‑141 (MQ=255)
                                                                                                       gATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGAAAAGAGACAtt                               <  1:765978/141‑1 (MQ=255)
                                                                                                             tactTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGCAAAGAGACATTACGCTa                         >  2:519578/1‑141 (MQ=255)
                                                                                                             tactTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTAGGTTTTTTATTGTTAATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTAAATTCTTTAAGATATTCGTGAAAAGAGACATTACACTa                         >  2:230952/1‑141 (MQ=255)
                                                                                                              actTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGAAAAGAGACATTACACTAg                        <  2:110452/141‑1 (MQ=255)
                                                                                                                ttGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGCTAACCGGCAAAAGAGCCATTACACTAGtt                      >  2:741459/1‑141 (MQ=255)
                                                                                                                ttGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGAAAAGAGACATTACACTAGtt                      <  1:655185/141‑1 (MQ=255)
                                                                                                                ttGATCATTACAAACCAAGTGCA‑ACTTTCTGCACCACGTTTTTTACTGTTTATAAATAAAACTCACTTAATAACGTTTTCATAATCTTCTTCAACTACATAATTCTTTAAGATATTCCTGAAAAGAGACATTACACCAGtc                      <  2:288417/141‑2 (MQ=255)
                                                                                                                 tGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGAAAAGAGACATTACACTAGTTa                     <  1:159339/141‑1 (MQ=255)
                                                                                                                   aTCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAACTAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATTTTCGTGAAAAGATACATTACACTAGTTAAt                   >  2:491113/1‑141 (MQ=255)
                                                                                                                     cATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGAAAAGAGACATTACACTAGTTAAttt                 >  2:410540/1‑141 (MQ=255)
                                                                                                                      ataaaaAACCAAGTGCAGAAAGT‑TGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGAAAAGAGACATTACACTAGTTAAtttt                >  2:736922‑M1/7‑141 (MQ=255)
                                                                                                                                AAGTGCA‑ACTTTCTGTACTTGGTTTTTTATTGTTTATGAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGAAAAGAGACATTACACTAGTTAATTTTCAAACTATAc      >  1:547626‑M1/1‑141 (MQ=255)
                                                                                                                                 AGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGAAAAGAGACATTACACTAGTTAATTTTCAAACAATACa     >  1:281233‑M1/1‑141 (MQ=255)
                                                                                                                                    GCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGAAAAGAGACATTACACTAGTTAATTTTCAAACAATACaaaa  <  1:99265‑M1/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                       
GTGTTAGTTGTTCGTACTGAAGAGTTACCAGCAGACTTCCAACCACAAGTTGCAACAACTCAATTACGTGAAAAATATCAAAATTAAATATAGATGATCATTAGATAACTACTTGATCATTATGAACCAAGTGCA‑ACTTTCTGCACTTGGTTTTTTATTGTTTATAAATAAAACTCACTTAATAATGTTTTCATAATCTTCTTCGACTACTTAATTCTTTAAGATATTCGTGAAAAGAGACATTACACTAGTTAATTTTCAAACAATACAAAA  >  CP000730/1822593‑1822865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑