Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,004,413 T→C 6.0% I65M (ATA→ATG USA300HOU_1856 ← Fur family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,004,4130TC 6.0% 187.6 / 3.2 67I65M (ATA→ATGUSA300HOU_1856Fur family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (31/32);  new base C (2/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.06e-01

AAAATCAACAATCTTACCACATTGTTCACATATAATATGATAATGATTATGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCTTGTCTTTGAGGTGTAATTCTTACGCCTGCTTGTCGCA  >  CP000730/2004275‑2004552
                                                                                                                                          |                                                                                                                                           
aaaaTCAACAATCTTACCACATTGTTCACATATAATATGATAATGATTATGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTatatat                                                                                                                                           >  2:676176/1‑141 (MQ=255)
 aaaTCAACAATCTTACCACATTGTTCACATATAATATGATAATGATTATGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATAtt                                                                                                                                          >  2:729986/1‑141 (MQ=255)
  aaTGAAAAATTTTACCCCATTGTTCACATATAAGTAGATAATGATTATGTGTATTAATGTCGAATCGCCTTGATGAGTCTCACTATGTTAATTCTTTTACACTTCCATTCTCTTTAAACTCTCTTAAGTTATTATATATTg                                                                                                                                         <  1:352740/141‑1 (MQ=255)
  aaTCAACAATCTTACCACATTGTTCACATATAATATGATAATGATTATGTGTATTAAAGTCGAATCGACTTTATGAGTTTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTg                                                                                                                                         <  2:259868/141‑1 (MQ=255)
   aTCAAAAATCTTACCCCATTGTTCACTTATAATATTATAATGATTATGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGt                                                                                                                                        <  2:150526/141‑1 (MQ=255)
        caaTCTTACCACATTGTTCACATATAATATGATAATGATTATGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCaa                                                                                                                                   >  1:168066/1‑141 (MQ=255)
          aTCTTACCACATTGTTCACATATAATATGATAATGATTATGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAaca                                                                                                                                 <  1:676176/141‑1 (MQ=255)
                 cacaCTGTTCACATATAATATGATAATGATTATGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTatat                                                                                                                          <  1:295167/141‑1 (MQ=255)
                  acaTTGTTCACATATAATATGATAATGATTATGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATAtt                                                                                                                         >  1:237442/1‑141 (MQ=255)
                       gTTCACATATAATATGATAATGATTATGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGaa                                                                                                                    >  1:191739/1‑141 (MQ=255)
                         tCACATATAATATGATAATGATTATGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGaaaa                                                                                                                  >  1:677903/1‑141 (MQ=255)
                            caTATAATATGATAATGATTATGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCa                                                                                                               <  1:632629/141‑1 (MQ=255)
                                taataTGATAATGATTATGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATAAGGTg                                                                                                           >  1:108022/1‑141 (MQ=255)
                                 aataTGATAATGATTATGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGa                                                                                                          <  2:592417/141‑1 (MQ=255)
                                       aTAATGATTATGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGc                                                                                                    <  2:351012/141‑1 (MQ=255)
                                            gATTATGTGTATTAAAGTCGAATCGTCCACATTAGGCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGAt                                                                                               <  2:743131/141‑1 (MQ=255)
                                               tATGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATaaa                                                                                            >  1:416241/1‑141 (MQ=255)
                                                aTGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAAt                                                                                           >  2:677706/1‑141 (MQ=255)
                                                 tgtgTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTGTATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAAtt                                                                                          >  1:173684/1‑141 (MQ=255)
                                                 tgtgTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAAtt                                                                                          >  2:301771/1‑141 (MQ=255)
                                                 tgtgTATTAAAGTCGAAGCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAAtt                                                                                          <  2:324820/141‑1 (MQ=255)
                                                    gtATTAAAGTCGAATCGACTAGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATAAGGTGAAAGTGCTTGATAAATTtca                                                                                       >  2:471409/1‑141 (MQ=255)
                                                     tATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTtcat                                                                                      >  2:604080/1‑141 (MQ=255)
                                                         aaaGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGc                                                                                  <  1:394647/141‑1 (MQ=255)
                                                          aaGTCGAATCGACTTGATTAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCt                                                                                 <  1:125492/141‑1 (MQ=255)
                                                          aaGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCt                                                                                 <  1:322457/141‑1 (MQ=255)
                                                           aGTCGAATCGACTTGATGATGCTCCATATGTTAATTCTTTTACAATTCCAATATCTTGAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTg                                                                                <  2:669415/141‑1 (MQ=255)
                                                                   cGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGGAAATCAGGTGAAAGTGCTTGATAAATTTCATAAGCTGTTGGATgg                                                                        >  2:651906/1‑140 (MQ=255)
                                                                   cGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGa                                                                        >  1:267984/1‑141 (MQ=255)
                                                                       tatatgcGTCTCACTATGTTAATTCTTATAAAATTACTATATCTTTAAACGCTCATAAGTAATTATATATTGTCGCAACACGTATCTTGGGAAAATCAGATGAAAGTGCATGATAAAATTCATCAGCTGTTGGATGAGTAt                                                                    <  2:717795/138‑1 (MQ=255)
                                                                          atgaGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTAtgtg                                                                 >  2:58601/1‑141 (MQ=255)
                                                                           tgaGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATAATATATAGTCTCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTga                                                                >  1:105895/1‑141 (MQ=255)
                                                                           tgaGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTATTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGTTAAATTTCATCAGCTGTTGGATGGGGATGTga                                                                >  2:13927/1‑141 (MQ=255)
                                                                            gaGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTgaa                                                               <  2:248568/141‑1 (MQ=255)
                                                                              gTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTgaaga                                                             >  2:289890/1‑141 (MQ=255)
                                                                               tctcCATATGTTAATTCTTTTACAATTCCAATATCTTTAAAGACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGAATGTgaagaa                                                            >  1:530723/1‑141 (MQ=255)
                                                                                   cATATGTTAATTCTTTTACAATTCTAATATCTTTAAACACTCTTACGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTa                                                        <  1:716403/141‑1 (MQ=255)
                                                                                   cATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTa                                                        <  1:550039/141‑1 (MQ=255)
                                                                                    atatGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTaa                                                       <  2:136058/141‑1 (MQ=255)
                                                                                     tatGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCCGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAGATGaaa                                                      >  1:634707/1‑141 (MQ=255)
                                                                                     tatGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTaaa                                                      >  1:486935/1‑141 (MQ=255)
                                                                                     tatGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTaaa                                                      >  1:746620/1‑141 (MQ=255)
                                                                                       tGTTAATTCTTTTACAATTCCAATATCTTTAAACTCTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGTTGTTGGATGAGTATGTGAAGAAATTAAATa                                                    <  2:763225/141‑1 (MQ=255)
                                                                                        gTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTGTATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGTTGTTGGATGAGTATGTGAAGAAATTAAATaa                                                   >  2:502079/1‑141 (MQ=255)
                                                                                         ttAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAAc                                                  <  1:88926/141‑1 (MQ=255)
                                                                                           aaTTCTTTTACAATTTCAATATCTTTAAACACTCTTAAGTTATTATACATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGt                                                <  1:116935/141‑1 (MQ=255)
                                                                                           aaTTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATACATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGt                                                <  1:135394/141‑1 (MQ=255)
                                                                                            aTTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTa                                               <  2:516274/141‑1 (MQ=255)
                                                                                            aTTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATACATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAAAAACGTa                                               >  2:725679/1‑141 (MQ=255)
                                                                                            aTTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATACATTGTAGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCTGCTGTAGGAAGACTATGTGAAGAAATAAAATAACGTa                                               >  2:327519/1‑141 (MQ=255)
                                                                                               tttttACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAata                                            <  1:626461/140‑1 (MQ=255)
                                                                                                 tttACAATTCCAATAACTTTAAACACTCTCAAAGTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTAAAGAAATTAAATAACGTAATAtt                                          >  2:321741/1‑141 (MQ=255)
                                                                                                   tACAAATCCAATATCTTTAAACACTCTTTAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGc                                        <  2:19841/141‑1 (MQ=255)
                                                                                                    aCAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCt                                       >  1:762226/1‑141 (MQ=255)
                                                                                                    aCAATTCCAATATCTTTAAAAACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAATTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCt                                       >  2:297913/1‑141 (MQ=255)
                                                                                                    aCAAGTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCt                                       <  2:524683/141‑1 (MQ=255)
                                                                                                     aaaTTCCAATATCTTTAAACACTTTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCtt                                      <  2:370542/140‑1 (MQ=255)
                                                                                                         tCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCTTGTCt                                  <  2:118629/141‑1 (MQ=255)
                                                                                                               atCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAACGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCTTGTCCTTGaag                            >  2:307069/1‑139 (MQ=255)
                                                                                                                tCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTCATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCTTGTCTTTGAGgt                           >  2:557538/1‑141 (MQ=255)
                                                                                                                  tttAAACTCTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCTTGTCTTTGAGgtgt                         <  1:505409/141‑1 (MQ=255)
                                                                                                                  tttAAACACTCTTAAGTTATTATTTATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCTTGTCTTTGAGgtgt                         <  1:678631/141‑1 (MQ=255)
                                                                                                                     aaaCACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCTTGTCTTTGAGGTGTAAt                      >  2:613065/1‑141 (MQ=255)
                                                                                                                              tAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCTTGTCTTTGAGGTGTAATTCTTACGcc             <  1:301771/141‑1 (MQ=255)
                                                                                                                               aaGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCTTGTCTTTGAGGTGTAATTCTTACGCCt            >  1:302734/1‑141 (MQ=255)
                                                                                                                               aaGTTATTATATATTGTCGCAACACTTATATTTCGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCTTGTCTTTGAGGTGTAATTCTTACGCCt            <  2:432934/141‑1 (MQ=255)
                                                                                                                                    attatATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCTTGTCTTTGAGGTGTAATTCTTACGCCTGCTTg       >  1:480397/1‑141 (MQ=255)
                                                                                                                                     ttatATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCTTGTCTTTGAGGCGTAATTCTTACGCCTGCTTGt      <  1:729986/141‑1 (MQ=255)
                                                                                                                                       atatatTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCTTGTCTTTGAGGTGTAATTCTTACGCCTGCTTGTCg    <  2:624859/141‑1 (MQ=255)
                                                                                                                                         atatTGGCGCAACACTTATATTTGGAAAATCAGATGAAAGTGCTTGATAAATTCCGTCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTACTATTGCTTGTCTTTGAGGTGTAATTCTTACGCCTGCTTGTCGCa  <  2:425080/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AAAATCAACAATCTTACCACATTGTTCACATATAATATGATAATGATTATGTGTATTAAAGTCGAATCGACTTGATGAGTCTCCATATGTTAATTCTTTTACAATTCCAATATCTTTAAACACTCTTAAGTTATTATATATTGTCGCAACACTTATATTTGGAAAATCAGGTGAAAGTGCTTGATAAATTTCATCAGCTGTTGGATGAGTATGTGAAGAAATTAAATAACGTAATATTGCTTGTCTTTGAGGTGTAATTCTTACGCCTGCTTGTCGCA  >  CP000730/2004275‑2004552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: