Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,420,667 G→T 8.6% intergenic (+98/‑264) ssaA → / → USA300HOU_2286 secretory antigen SsaA/(R)‑2‑hydroxyacid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,420,6670GT 8.6% 227.2 / 2.2 79intergenic (+98/‑264)ssaA/USA300HOU_2286secretory antigen SsaA/(R)‑2‑hydroxyacid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (35/36);  new base T (6/2);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTGCAAGTGAAGCAGGTAACTACAACTACATCCATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATCCAGTTTTTCCACATTACAATATTCATAGT  >  CP000730/2420527‑2420802
                                                                                                                                             |                                                                                                                                       
ttCTGCAAGTGAAGCAGGTAACTACAACTACATCCATATTTAATGTAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTCTGAGCTTTCCTTTATAGGGGAAGCTCTTTTTTTg                                                                                                                                         >  1:470394/1‑141 (MQ=255)
   tGCAAGTGAAGCAGGTAACTACAACTACATCCATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCtt                                                                                                                                      >  2:229744/1‑140 (MQ=255)
      aaGTGAAGCAGGTAACTACAACTACATCCATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTAttt                                                                                                                                   <  1:716254/141‑1 (MQ=255)
          taaGCAGGTAACTACAACTACATCCATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCg                                                                                                                               >  2:756546/2‑141 (MQ=255)
              cAGGTAACTACAACTACATCCATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGc                                                                                                                           <  1:58564/141‑1 (MQ=255)
                 gTAACTACAACTACATCCATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGTTATTTTTGGTTGcccc                                                                                                                        >  2:33282/1‑139 (MQ=255)
                 gTAACTACAACTACATCCATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGTTATTTTTCGTTGCCAc                                                                                                                        >  2:711918/1‑141 (MQ=255)
                 gTAACTACAACTACATCCATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCAc                                                                                                                        >  1:497172/1‑141 (MQ=255)
                 gTAACTACAACTACATCCATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCAc                                                                                                                        <  1:90291/141‑1 (MQ=255)
                 gTAACTACAACTACATCCATATTTAATATAAACACGAGGATACATATGGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCAc                                                                                                                        >  2:374250/1‑141 (MQ=255)
                  tatcgaaaactacaTCCTGTTTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATAGGTCATCAATAAGCTTTGAGCTTTCCTTTATAGGAGCAGATCTTGTTGTTCTATTTTTCGTTGCCAcc                                                                                                                       <  2:356168/134‑1 (MQ=255)
                  tAACTACAATTACATCCATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCAcc                                                                                                                       <  2:185766/141‑1 (MQ=255)
                   aactacaactacATCCATATTTAATATATACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGCGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCt                                                                                                                      <  1:538178/141‑1 (MQ=255)
                        caactacaTCCATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTACCACCTACtta                                                                                                                 <  1:756546/141‑1 (MQ=255)
                          actacaTCCATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACttatt                                                                                                               <  1:162064/141‑1 (MQ=255)
                           ctacaTCCATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACttattt                                                                                                              <  2:351269/141‑1 (MQ=255)
                            tacaTCCATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACttattta                                                                                                             <  1:650888/141‑1 (MQ=255)
                              caTCCATATTTAATATAAACACGATGATACATATTGTCTTGAAGCTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTtata                                                                                                           <  1:598300/141‑1 (MQ=255)
                              caTCCATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTtata                                                                                                           <  1:50780/141‑1 (MQ=255)
                              actCCATTTTTAATGTAAACACGATGATACAGATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTGATTtata                                                                                                           <  2:339617/139‑1 (MQ=255)
                                  cATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTTCTATTTTTCGTTGCCACCTACTTATTTATAtttt                                                                                                       >  2:708655/1‑140 (MQ=255)
                                  cATATTAAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACACATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTACTTATATTtc                                                                                                       >  2:487707/1‑141 (MQ=255)
                                    tatTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTtctt                                                                                                     >  1:756346/1‑139 (MQ=255)
                                       ttAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTtcctcc                                                                                                   >  1:231008/1‑141 (MQ=255)
                                         aaTATAAACACGATTATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAACAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCat                                                                                                <  2:50723/141‑1 (MQ=255)
                                          atatAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTTTTTTTTTTTGTTGCAACCTACTTATTTATATTTCCTcccttt                                                                                               >  2:107045/1‑138 (MQ=255)
                                                  acGATGATACATATTGTCTTGAAACTTAATTCTCGTACATATGTCATCAAAAATCGTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTCTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAat                                                                                       <  2:424024/141‑1 (MQ=255)
                                                  acGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAat                                                                                       >  1:333645/1‑141 (MQ=255)
                                                  acGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGGCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAat                                                                                       <  1:143279/141‑1 (MQ=255)
                                                   cGATGACACATACTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAata                                                                                      <  2:740253/141‑1 (MQ=255)
                                                     atgatACATATTGTCTTGAAACTTAATTCTACTACATATGTCATCAAAAAACGTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATAtt                                                                                    <  2:326548/141‑1 (MQ=255)
                                                       gatACATATTGTCTTGAAACTTAATTCTAGTATTTATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCTTCCCATATTCAAAATATTaa                                                                                  >  1:373628/1‑141 (MQ=255)
                                                       gatACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTCTTTATATTTCCTCCCATATTCAAAATATTaa                                                                                  >  1:432891/1‑141 (MQ=255)
                                                          tcATATTGTCTTGAAACTTAATTCTAGTACATATCTCATCAAAAAGCTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAAttt                                                                               <  2:43442/140‑1 (MQ=255)
                                                                    ttGAAACTTAATTCTAGTACTTATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATAc                                                                     <  2:120641/141‑1 (MQ=255)
                                                                      gAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTa                                                                   >  1:416807/1‑141 (MQ=255)
                                                                           ttAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTTTAATACTATTAAc                                                              >  1:197079/1‑141 (MQ=255)
                                                                           ttAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAAc                                                              >  1:584286/1‑141 (MQ=255)
                                                                            tAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACt                                                             >  2:52026/1‑141 (MQ=255)
                                                                            tAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACt                                                             >  1:341127/1‑141 (MQ=255)
                                                                            tAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACt                                                             >  1:492962/1‑141 (MQ=255)
                                                                             aaTTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACtt                                                            <  2:562121/141‑1 (MQ=255)
                                                                               ttCTCGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCGTATTCAAAATATTAATTTTTTCTAATACTTTTAACTTTa                                                          >  1:526254/1‑141 (MQ=255)
                                                                               ttCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTa                                                          <  2:52513/141‑1 (MQ=255)
                                                                               ttCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTa                                                          <  2:52450/141‑1 (MQ=255)
                                                                                 cTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTACTACTCTTAATTTTcaa                                                        >  2:16619/1‑141 (MQ=255)
                                                                                   aGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAAGAGAAGATCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAAc                                                      >  2:503273/1‑141 (MQ=255)
                                                                                    gTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAACAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACt                                                     >  1:150346/1‑141 (MQ=255)
                                                                                      aCATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTAc                                                   <  1:125790/141‑1 (MQ=255)
                                                                                      aCATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTAc                                                   <  1:714972/141‑1 (MQ=255)
                                                                                         tatGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTTATACTATTATCTTTAAAACTACGtt                                                <  1:387602/141‑1 (MQ=255)
                                                                                          atGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTTCTTTTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCTAAATATTAATTTTTTCTAATTCTATTAACTTTAAAACTACGttt                                               >  2:107965/1‑140 (MQ=255)
                                                                                           tGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTAt                                              >  1:304630/1‑141 (MQ=255)
                                                                                           tGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTGTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTAt                                              <  2:4357/141‑1 (MQ=255)
                                                                                             tcatcaAAACACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCGCCTACTTATTTATATTTCCTCCCATATTCATAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCt                                            <  2:369903/141‑1 (MQ=255)
                                                                                              catcaAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCta                                           <  2:636260/141‑1 (MQ=255)
                                                                                                   aaaaaCTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTTTTTTTTTTTTTTGCCAACTACGTATTTATATTTCCACCCGTATTAACAATATTAATTTGTTCTCATTCCATTAATTTTTAAATCACGTTatttaattta                                      >  2:120166/1‑141 (MQ=255)
                                                                                                     aaaCTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTTCTTTTTTTCCTTGGCACCTACTTATTTATTTTTTCTCCCATATTTAAAATATTAATTTTTTTTAATACTATTAACTTTAAAACTAAGTTATtttattttat                                    >  1:778696/1‑141 (MQ=255)
                                                                                                     aaaCTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTTTAATACTATTAACTTTAAAACTACGATTTCtaatttcat                                    >  2:460692/1‑141 (MQ=255)
                                                                                                       aCTTGGAGCTTTCCTTTATATGATGAGTTCTTGTTTTTCTCTTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCATAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTg                                  <  2:454750/141‑1 (MQ=255)
                                                                                                          ttGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATc                               <  1:739221/141‑1 (MQ=255)
                                                                                                           tGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATAACTTTAAAACTACGTTATCTAATTTAATTGATCCa                             <  2:572391/141‑1 (MQ=255)
                                                                                                            gAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATTCa                             >  1:641521/1‑141 (MQ=255)
                                                                                                             aGCTTTCCTTTATAGAAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATCCAg                            >  1:577549/1‑141 (MQ=255)
                                                                                                              gCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATCCAGt                           <  2:585923/141‑1 (MQ=255)
                                                                                                                 ttCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATCCAGtttt                        >  1:515188/1‑141 (MQ=255)
                                                                                                                    cTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATCCAGTTTTTcc                     >  1:580718/1‑141 (MQ=255)
                                                                                                                     tttATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATCCAGTTTTTCca                    <  2:150346/141‑1 (MQ=255)
                                                                                                                        ataGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATCCAGTTTTTCCACAt                 <  1:96378/141‑1 (MQ=255)
                                                                                                                           ggAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATCCAGTTTTTCCACATTAc              <  2:430216/141‑1 (MQ=255)
                                                                                                                            gagaAGCTCTTTTTTTTTTTTTTTTCTTTGCCACATACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATTCTATTAACTTTAAAACTACGTAATCTAATTTAATTTATCTATTTTTTCCACATcaca             >  2:81822/1‑141 (MQ=255)
                                                                                                                             agaAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATCCAGTTTTTCCACATTACaa            >  1:538203/1‑141 (MQ=255)
                                                                                                                              gaAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATCCAGTTTTTCCACATTACaaa           >  1:635338/1‑140 (MQ=255)
                                                                                                                              gaAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATCCAGTTTTTCCACATTACAat           >  1:84550/1‑141 (MQ=255)
                                                                                                                               aaGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTCACTTTAAAACTACGTTATCTAATTTAATTGATCCAGTTTTTCCACATTACAata          <  1:503273/141‑1 (MQ=255)
                                                                                                                                aGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATCCAGTTTTTCCACATTACAatat         <  2:416807/141‑1 (MQ=255)
                                                                                                                                  ctctTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATCCAGTTTTTCCACATTACAATATTc       >  1:595710/1‑141 (MQ=255)
                                                                                                                                   tctTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCACATATTCAAAATATTCATTTTTTCTAATACTTTTCACTTTAAAACTACGTTATCTAATTTCATTTATCCAGTTTTTCCACATTCCACTATTct      >  2:224342/1‑140 (MQ=255)
                                                                                                                                    ctTTTTTTGCTATTTTTCGTTGCCACCTACTTCTTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTCATCTAATTCAATTGATCCGGTTTTTCTACCTTCCAATCTTCAt     >  2:305671/1‑141 (MQ=255)
                                                                                                                                      tttttttGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATCTTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTGCGTTCTCTAATTGAATTGATCCAGTTTTTCCACTTTACAATCTTCATa    >  1:212954/1‑141 (MQ=255)
                                                                                                                                        ttttttCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATCCAGTTTTTCCACATTACAATATTCATAGt  <  2:303879/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                       
TTCTGCAAGTGAAGCAGGTAACTACAACTACATCCATATTTAATATAAACACGATGATACATATTGTCTTGAAACTTAATTCTAGTACATATGTCATCAAAAAACTTTGAGCTTTCCTTTATAGGAGAAGCTCTTTTTTTGCTATTTTTCGTTGCCACCTACTTATTTATATTTCCTCCCATATTCAAAATATTAATTTTTTCTAATACTATTAACTTTAAAACTACGTTATCTAATTTAATTGATCCAGTTTTTCCACATTACAATATTCATAGT  >  CP000730/2420527‑2420802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: