Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 111,560 T→C 10.3% R210R (CGT→CGC USA300HOU_0110 → possible staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730111,5600TC10.3% 229.1 / 11.2 87R210R (CGT→CGCUSA300HOU_0110possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (48/28);  new base C (2/7);  total (51/36)
Fisher's exact test for biased strand distribution p-value = 2.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGA  >  CP000730/111421‑111700
                                                                                                                                           |                                                                                                                                            
ttAAGTTTTTTGCGCAAGATAGCAAGTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTa                                                                                                                                             <  1:314339/141‑1 (MQ=255)
 tAAGTTTTTTGCGTAATATAGCAATTTTAAAGATTTAATGAAGTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                                                                                                                            <  1:601283/141‑1 (MQ=17)
 tAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                                                                            >  1:685925/1‑141 (MQ=255)
 tAAATTTTTGGCGGAATACTGCCACTTTAAATATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                                                                            <  2:158597/141‑1 (MQ=17)
   aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaaaa                                                                                                                                          >  1:650130/1‑141 (MQ=255)
           gcgcAATATAGCAATCTTAAAGATTTCATGAATTCTAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTATCTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGat                                                                                                                                  <  1:470277/141‑2 (MQ=14)
             gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGataa                                                                                                                                >  2:600571/1‑138 (MQ=255)
             gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACAt                                                                                                                                <  1:300482/141‑1 (MQ=255)
              cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                                                                               <  1:106475/141‑1 (MQ=255)
               aaTATAGTAATTTTAAACATTTAATTAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAc                                                                                                                              <  1:680374/141‑1 (MQ=11)
                 tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccg                                                                                                                            <  2:243920/141‑2 (MQ=14)
                  ataGCAATTTTAAAGATTTAATGAAGTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTACTGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccga                                                                                                                           <  2:427265/141‑3 (MQ=14)
                   aaGCAATTTTAAAGATTTAATGAAATATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaac                                                                                                                          <  2:418520/140‑1 (MQ=255)
                    aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacg                                                                                                                         <  1:397643/141‑5 (MQ=11)
                    aGCAATTTTAAAGATTTAACGAGCTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGGAAAATATATGATATACcgacg                                                                                                                         <  1:397730/141‑2 (MQ=11)
                     gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATGTAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                                                                        >  2:649307/1‑141 (MQ=255)
                     gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacga                                                                                                                        <  1:518243/141‑6 (MQ=11)
                          tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaaa                                                                                                                   <  1:630484/141‑1 (MQ=255)
                           ttAAAGATTTAATGCATTATAAAGATGGATATATATCGTATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAGAAAATTACGCACAAGATAGGAtataaagacgattaaag                                                                                                                  >  2:579634/1‑128 (MQ=11)
                             aaaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAATGATATGACATACCAACAAATAAAgcg                                                                                                                >  1:468467/1‑141 (MQ=255)
                              aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAGTGTAAAAAAATTACGTAAAAGAAATGACATACCAACAAAGAAAgcgc                                                                                                               >  1:152114/1‑141 (MQ=255)
                              aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcgc                                                                                                               >  1:707411/1‑141 (MQ=255)
                                 aTTTATTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGa                                                                                                            >  2:69763/1‑141 (MQ=255)
                                  tttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAACGCCGaa                                                                                                           >  2:78521/1‑141 (MQ=255)
                                     aaTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCt                                                                                                        <  1:637238/141‑1 (MQ=255)
                                       tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGGGCCGAAGCtgt                                                                                                      >  2:720723/1‑141 (MQ=255)
                                        gAATTAAAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgtt                                                                                                     <  2:752493/141‑1 (MQ=255)
                                          aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGGGCCGAAGCtgttgt                                                                                                   >  2:468676/1‑141 (MQ=255)
                                            tataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGttgttg                                                                                                 <  2:360616/141‑1 (MQ=255)
                                            tataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGttgttg                                                                                                 <  2:477261/141‑1 (MQ=255)
                                             ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGa                                                                                                >  1:298958/1‑141 (MQ=255)
                                             ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGa                                                                                                >  1:298736/1‑141 (MQ=255)
                                                aaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAg                                                                                             >  1:706851/1‑141 (MQ=255)
                                                 aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAgg                                                                                            >  1:268135/1‑141 (MQ=255)
                                                  gATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGt                                                                                           <  1:256941/141‑1 (MQ=255)
                                                  gATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGAAATAACCACAAAACAAGCGCAGAAGCtttcggtcaaatgt                                                                                           <  2:650130/141‑14 (MQ=17)
                                                            tatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAtt                                                                                 <  2:305257/141‑1 (MQ=255)
                                                            tatCATATAATCCAGAGGTGCCGAGCTATTCAGCTCAATATCAAGTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAtt                                                                                 >  1:424130/1‑141 (MQ=255)
                                                              tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATCCCAACAAATAGAGCGCCGAAGCTGTTGTTACAAGGTAAAGGGAATTTa                                                                               >  2:436644/1‑141 (MQ=17)
                                                              tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTa                                                                               >  1:588603/1‑141 (MQ=255)
                                                              tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATACTGAGTATAATGTAAAACACTTACGTAAAAGAGCCGAGAAACCAACAAAAAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTa                                                                               >  2:163044/1‑141 (MQ=17)
                                                               cATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGTTTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTCAAAGGTACAGGGAATTTaa                                                                              <  1:433807/141‑1 (MQ=255)
                                                                atatAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGATGTGGCGGAAAGGTACAGGGAATTTaaa                                                                             <  2:550642/141‑1 (MQ=255)
                                                                    aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                                                                         <  1:555620/141‑1 (MQ=255)
                                                                      tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACGGGGAATTTAAAAGGTtc                                                                       >  1:301285/1‑141 (MQ=255)
                                                                       ccAGGGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAAAGCAAATTAAGTACGAAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                      >  1:529337/1‑141 (MQ=14)
                                                                       ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGGGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                      >  1:701163/1‑141 (MQ=255)
                                                                          gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatca                                                                   <  1:377412/141‑1 (MQ=255)
                                                                          gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGGTGAAAGGTACAGGGAATTTAAAAGGTtcataa                                                                   >  2:26347/1‑139 (MQ=255)
                                                                            ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGt                                                                 >  1:446192/1‑141 (MQ=255)
                                                                            ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGt                                                                 >  2:238474/1‑141 (MQ=255)
                                                                             gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTGTAATGTAAAACACTTACGTAAAAGATATGACATACAAAAAAATAAAGCGCCGAAGATATTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGtt                                                                >  2:710040/1‑141 (MQ=14)
                                                                               gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCAGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCAACAGTTgg                                                              >  2:77495/1‑141 (MQ=255)
                                                                                 cGAGTTGTTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGGACAGGGAATTTAAAAGGTTCATCAGTTGGat                                                            >  2:427267/1‑141 (MQ=255)
                                                                                 cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATAAGTTGGat                                                            >  1:169825/1‑141 (MQ=255)
                                                                                 cGAGTTATTCAGCTCAATATCAAATAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCTCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGat                                                            <  2:187980/141‑1 (MQ=255)
                                                                                  aaGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCGGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGata                                                           <  1:261630/140‑1 (MQ=255)
                                                                                   aGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTCTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAgaggggtag                                                          >  2:640303/1‑133 (MQ=255)
                                                                                    gTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGAtata                                                         >  1:734639/1‑141 (MQ=255)
                                                                                     ttattaAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAATGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaa                                                        <  2:640769/141‑1 (MQ=255)
                                                                                     ttATTCAGCTTAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaa                                                        <  1:248423/141‑1 (MQ=255)
                                                                                         tttctgcAATATCAATTAACTAATGATGATTATAATGTAAATCAATGACGTCATAGTTCAGACATGTCTACAATTAAAGCGCCTGAGCTTTTGTTGAATGGTACAGGGAATTTAAAAGGTTCATCAGTTCGATATaaaaaa                                                    <  2:379917/135‑1 (MQ=9)
                                                                                         tCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaaa                                                    >  2:181290/1‑141 (MQ=255)
                                                                                           aGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAt                                                  >  1:659343/1‑141 (MQ=255)
                                                                                            gCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGGACAAGGAATTTAAAAGGTTCATCAGTTGGATATAAAAACAtt                                                 >  1:775262/1‑141 (MQ=255)
                                                                                              tCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGa                                               >  1:426020/1‑141 (MQ=255)
                                                                                               aaaTATCAATGAACTAATGAATATTATAAAAAAAAAAAATTACTTAAAATTTTGGAGAGACCAACAAAGAAAGCGCCGAAGAGGTTGTTGAAAGGTACAGGGAATTTAAAATGTTCATCAGATAAAAAAAAAAAAATTGaa                                              <  2:182443/140‑1 (MQ=255)
                                                                                                  tatCGATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGAGACTTGAAAAGGTTCATCAGTTGGATAGAAAAAAATTGAAttt                                           >  1:547807/1‑141 (MQ=17)
                                                                                                   atCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAGAAAATTGAATTTa                                          >  1:171775/1‑141 (MQ=255)
                                                                                                   atCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTa                                          >  1:677245/1‑141 (MQ=255)
                                                                                                   atCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTa                                          >  1:218639/1‑141 (MQ=255)
                                                                                                       aTTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTGGGATATAAAAAAATTGAATTTACttt                                      >  1:169662/1‑141 (MQ=255)
                                                                                                                tgatgcTTATAATGTAAAGCACTTGCTTAAAAGATATGACCTACCAACAAATAAAGCGCCGAAGCTATTGTTGAAAGGTACAGGGAATTTAAGAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGaa                             >  1:571488/1‑141 (MQ=17)
                                                                                                                 gatgatTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGACGCTGTTGTTGAAAGGTACAGCGAATTTAACAGGTTCATCAGTTGGTTATAAAAAAATTGAATTTACTTTTTTAGAGAAt                            >  2:733686/1‑141 (MQ=255)
                                                                                                                   tgatTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaa                          >  2:176281/1‑141 (MQ=255)
                                                                                                                    gatTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaaa                         <  1:394813/141‑1 (MQ=255)
                                                                                                                      ttATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaaaaa                       <  1:709961/141‑1 (MQ=255)
                                                                                                                       tataATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTGGGATATAAAAAAATTGAATTTACTTTTTTAAAGAATaaaaag                      >  2:331847/1‑140 (MQ=255)
                                                                                                                              tAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAata               >  2:434865/1‑141 (MQ=255)
                                                                                                                                aaaCAATTACGTAAAAGATATGACATACCAACAAATAACGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtt             <  1:118998/141‑1 (MQ=255)
                                                                                                                                  aCAATTACGTAAAAGATATGACATACCAATAAATACAGCGTCGAAGCGGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGCGAATAAAATTGAAAATAttta           <  1:401246/141‑1 (MQ=18)
                                                                                                                                     aTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttactt        >  2:775206/1‑141 (MQ=255)
                                                                                                                                         cGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATttactttact    >  1:769257/1‑141 (MQ=255)
                                                                                                                                         cGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATttactttact    >  2:567624/1‑141 (MQ=255)
                                                                                                                                         cGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATttactttact    >  1:358657/1‑141 (MQ=255)
                                                                                                                                          gTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTg   >  1:79125/1‑141 (MQ=255)
                                                                                                                                          gTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTg   <  1:679808/141‑1 (MQ=255)
                                                                                                                                           tAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGa  >  2:609608/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGA  >  CP000730/111421‑111700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: