Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 367,644 G→T 100% intergenic (‑96/‑20) nanA ← / → USA300HOU_0336 N‑acetylneuraminate lyase/possible transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730367,6440GT100.0% 307.5 / NA 90intergenic (‑96/‑20)nanA/USA300HOU_0336N‑acetylneuraminate lyase/possible transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (45/45);  total (45/45)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACGAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTAGTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAATATGTT  >  CP000730/367508‑367779
                                                                                                                                        |                                                                                                                                       
aCGAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCat                                                                                                                                     <  1:99830/141‑1 (MQ=255)
aCGAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCat                                                                                                                                     >  1:210760/1‑141 (MQ=255)
 cGAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCata                                                                                                                                    <  1:737418/141‑1 (MQ=255)
  gAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACCAATCAGGTGGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCatat                                                                                                                                   >  1:563452/1‑141 (MQ=255)
   aGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATTATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATAtt                                                                                                                                  <  2:511246/141‑1 (MQ=255)
    gTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGACAATTGTATTCATAttt                                                                                                                                 >  1:556234/1‑141 (MQ=255)
     tAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTa                                                                                                                                >  1:496791/1‑141 (MQ=255)
      aaCGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTATAAGCTATCATAAAATGTAAAAATATCTTTTTGAAAATTATATTAATATTTAt                                                                                                                               >  2:744132/1‑141 (MQ=255)
       acagtGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATg                                                                                                                              <  2:379027/137‑1 (MQ=255)
       aCGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATg                                                                                                                              >  1:382389/1‑141 (MQ=255)
        agCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGt                                                                                                                             <  1:166380/140‑1 (MQ=255)
          cTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCTAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGtat                                                                                                                           >  2:413190/1‑141 (MQ=255)
            gCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCGCCAAAAATCATTTGATAATATAGTTTACAGCTATAAGTGGAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTAtaa                                                                                                                         <  1:742621/141‑1 (MQ=255)
                  aaaCCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATg                                                                                                                   >  2:110385/1‑141 (MQ=255)
                    aCCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGaa                                                                                                                 >  2:717437/1‑141 (MQ=255)
                    aCCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGaa                                                                                                                 >  2:717579/1‑141 (MQ=255)
                     ccTTTTAAATCTTTGTTCATTATGAAGTCCCTGCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGaaa                                                                                                                <  2:109865/141‑1 (MQ=255)
                       ttttgtaTCTTTTTCGATTATGAACTCCCCCGAAAAATCCTTTGATAATATAGCTTACAGCTATAATTGTAAACGCTATCATAAAATTGAACAATATCTTTTTTAAAATTGTATTCATATTTCCGTATAATTAATGACAAt                                                                                                              <  2:751780/135‑1 (MQ=255)
                         ttAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTCAACGCTAACATAAAATGTAACAATATCTTTTTGAATATTGTATTCATATTTATGTAGAATTAATGAAAATGt                                                                                                            <  1:575964/141‑1 (MQ=255)
                         ttAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGt                                                                                                            <  1:269841/141‑1 (MQ=255)
                         ttAAATCTTTGTTCATTATGAAGGCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGt                                                                                                            <  1:418555/141‑1 (MQ=255)
                          tAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGtt                                                                                                           <  2:677657/141‑1 (MQ=255)
                           aaaTCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGttt                                                                                                          <  1:227473/141‑1 (MQ=255)
                             aTCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGttttt                                                                                                        <  2:210760/141‑1 (MQ=255)
                               tttggTTCATTATGAAGTCCCTCCAAAGGTCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCa                                                                                                      <  2:228389/137‑1 (MQ=255)
                                tttGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCaa                                                                                                     >  2:192849/1‑141 (MQ=255)
                                 ttGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCaaa                                                                                                    >  1:619584/1‑141 (MQ=255)
                                   gTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCGATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCaaatt                                                                                                  >  2:375216/1‑139 (MQ=255)
                                       tttATGAAGTCCCTCCAAAAATCCTTTGATAATATAGTTTACAGCTATAATTGGAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAAt                                                                                              <  2:705119/140‑1 (MQ=255)
                                       aTTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTCTTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAAt                                                                                              <  1:732927/141‑1 (MQ=255)
                                         tATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAg                                                                                            <  2:195193/141‑1 (MQ=255)
                                          aTGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGa                                                                                           >  1:702608/1‑141 (MQ=255)
                                           tGAAGTCCCTCCCAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGaa                                                                                          <  1:294790/141‑1 (MQ=255)
                                           tGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGaa                                                                                          >  2:61550/1‑141 (MQ=255)
                                            gAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGaaa                                                                                         <  1:290267/141‑1 (MQ=255)
                                            gAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATAATTATGTATAATTAATGAAAATGTTTTTCAAAAACAAAAgcac                                                                                         >  2:336648/1‑138 (MQ=255)
                                            aaaGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGaaa                                                                                         <  1:679632/140‑1 (MQ=255)
                                             aaGTCCCTCCAAAAATCATTTGATCATATTGCTTCCATCTAAGATTGTACACGCTAGCATAGCGTGAGACGATATCGTGTGGAGAATTGTATGCAGATTTAGGGATAATTAATGAAAATGTTTTTCAAAATCAATAGAAAt                                                                                        <  2:449671/141‑1 (MQ=255)
                                             aaGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAAt                                                                                        <  2:758589/141‑1 (MQ=255)
                                             aaGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAAt                                                                                        >  2:157592/1‑141 (MQ=255)
                                              aGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATg                                                                                       >  1:345318/1‑141 (MQ=255)
                                               gTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATgg                                                                                      >  1:477087/1‑141 (MQ=255)
                                                tCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGga                                                                                     >  1:98015/1‑141 (MQ=255)
                                                  ccTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagt                                                                                   <  2:223061/141‑1 (MQ=255)
                                                  ccTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagg                                                                                   >  1:447223/1‑140 (MQ=255)
                                                     ccAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagtgag                                                                                >  1:714852/1‑141 (MQ=255)
                                                      cAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGgagtgaga                                                                               >  2:717869/1‑140 (MQ=255)
                                                       caaaaTCATTTGATAATGTAGTTTAAAGCTATAATTGTAAACGCTCTCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTa                                                                              <  2:604148/140‑1 (MQ=255)
                                                       aaaaaaCCTTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTa                                                                              <  2:601243/141‑1 (MQ=255)
                                                       aaaaaTCATTTGATAATATATTTTACAGCTATAATTGTAAACGCCATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTa                                                                              <  2:13443/141‑1 (MQ=255)
                                                       aaaaaTCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTa                                                                              <  1:139789/141‑1 (MQ=255)
                                                       aaaaaTCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTa                                                                              <  1:465897/141‑1 (MQ=255)
                                                         aaaTCATTTGATAATATAGTTTACAGTTATAATTGTAAACCTTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAg                                                                            <  2:118755/141‑1 (MQ=255)
                                                           aTCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGgt                                                                          <  1:715217/141‑1 (MQ=255)
                                                            ttaTTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGgtg                                                                         <  2:179417/139‑1 (MQ=255)
                                                                 tGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTa                                                                    >  1:669534/1‑141 (MQ=255)
                                                                       tataGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGATGGAGTAAGGTGTATTACATCGc                                                              >  1:689953/1‑141 (MQ=255)
                                                                        ataGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGATTGAGTAAGTTGTATTACATCGCa                                                             >  2:231887/1‑141 (MQ=255)
                                                                        ataGTTTACAGCTAGAATTGTAAACACTATCATAAAATGTAACAGTATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCa                                                             >  1:423709/1‑141 (MQ=255)
                                                                          aGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAAt                                                           >  1:338257/1‑141 (MQ=255)
                                                                           gTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATc                                                          >  1:272321/1‑141 (MQ=255)
                                                                           gTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATc                                                          >  2:694481/1‑141 (MQ=255)
                                                                           gTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATc                                                          >  1:585127/1‑141 (MQ=255)
                                                                           gTTTACAGCTAAAATTTTAAACACTATCATAAAATGTAATAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTCATGAAAATGTTTTTCAAAATCAATAGAAATGTAGTGAGTAAGGTGTATTACATCGCAATc                                                          <  1:108995/141‑1 (MQ=255)
                                                                            tttACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCg                                                         <  1:148669/141‑1 (MQ=255)
                                                                                cAGCTATAATTGTAAACGCTCTCATAAAATGTAACAATATCTTTTTGAAAATTGTATTTATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGataa                                                     >  1:469103/1‑140 (MQ=255)
                                                                                  gCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTTTTTTTTTTTTTTATATTTATGTATAATTAATGAAAATGTTTTTTAAATTCAATAGAAATGGAGTGTGTATGGTGTATTACATCTAAATTGATAttt                                                   >  2:152418/1‑140 (MQ=255)
                                                                                   cTATAATTGTAAACGCTATCATAGAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAAAGTTTTTCAAAATCAATAGAGATGGAGTGAGAAAGGTGTATGACGTCAGAATCGAAAGTgg                                                  >  1:238508/1‑141 (MQ=255)
                                                                                     ataATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTAAAATTAATGAAAATGTTTTTTAAAATCAATAGAAATGGAGTGAGCAAGGTGTATAACATCGCAATCGCTATTggtg                                                >  1:235321/1‑139 (MQ=255)
                                                                                      taATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAgg                                               >  1:345307/1‑141 (MQ=255)
                                                                                         ttGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCAc                                            >  2:631999/1‑141 (MQ=255)
                                                                                          tGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACt                                           <  1:744679/141‑1 (MQ=255)
                                                                                               aCGCTCTCATAAAATGTAACAATATCGTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAAGTGTATTACATCGCAATCGATATTGGAGGCACTCAAAt                                      <  1:643958/141‑1 (MQ=255)
                                                                                                   tATCATAAAATGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCAATATTGTAGGCACTAAAATTaaa                                  >  2:430105/1‑141 (MQ=255)
                                                                                                        aaaaaTGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGc                             <  1:719558/140‑1 (MQ=255)
                                                                                                           aaTGTAACAATATCTTTTTGAAAATTGTCTTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGt                          <  2:189933/141‑1 (MQ=255)
                                                                                                           aaTGTAACAATATCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGt                          <  1:176916/141‑1 (MQ=255)
                                                                                                                aaCAATATCTTTTTGAAAATAGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGACACTCAAATTAAATCGGCGGTTATTg                     >  1:472940/1‑141 (MQ=255)
                                                                                                                 aCAATATCTGTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGa                    <  2:684813/141‑1 (MQ=255)
                                                                                                                   aaTATCTTTTTGAAAATTGTATTCATATTTATGTATGATTAATGAAAATGTTTTTCAAAATCAGTAGAAATGGAGTGAGTAAGGTGTGTTACATCGCAATCGATATTGGAGGCACTCAGATTAAATCGGGAGATATTGATa                  >  2:115019/1‑141 (MQ=255)
                                                                                                                     tatCTTTTTGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAg                <  2:117670/141‑1 (MQ=255)
                                                                                                                       tCTTTTTGAATATTGTATTCGTATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCa              <  2:620588/141‑1 (MQ=255)
                                                                                                                          ttttGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCACtt           >  2:59517/1‑141 (MQ=255)
                                                                                                                           tttGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTg          <  1:157592/141‑1 (MQ=255)
                                                                                                                           tttGAAAATTGTATTCATATTTATGTATAAGTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTg          >  2:355178/1‑141 (MQ=255)
                                                                                                                             tGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGaa        <  2:563452/141‑1 (MQ=255)
                                                                                                                             tGAAAATTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGaa        >  1:189781/1‑141 (MQ=255)
                                                                                                                               aaaaTTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCGATCGATATTGAAGGCACTCAAATTAAATCGGCAGTTATTGCTTAGCAATGGAata      >  1:621962/1‑141 (MQ=255)
                                                                                                                                aaaTTGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAataa     >  2:737635/1‑140 (MQ=255)
                                                                                                                                   ttGTATTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAATATGtt  <  1:693014/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
ACGAGTAACGCTGCATATAAACCTTTTAAATCTTTGTTCATTATGAAGTCCCTCCAAAAATCATTTGATAATATAGTTTACAGCTATAATTGTAAACGCTATCATAAAATGTAACAATATCTTTTTGAAAATTGTAGTCATATTTATGTATAATTAATGAAAATGTTTTTCAAAATCAATAGAAATGGAGTGAGTAAGGTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAGCAATTGAATATGTT  >  CP000730/367508‑367779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: