Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 479,553 G→A 100% A194T (GCA→ACA)  USA300HOU_0458 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730479,5530GA100.0% 278.8 / ‑6.5 89A194T (GCA→ACA) USA300HOU_0458hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (46/42);  total (46/43)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTACCA  >  CP000730/479415‑479689
                                                                                                                                          |                                                                                                                                        
aGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGCTTAAATGGACAAAACAATATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACcataca                                                                                                                                        >  2:275439/1‑141 (MQ=255)
 gTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACcatacat                                                                                                                                       <  2:417167/141‑1 (MQ=255)
  tGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACcatacatg                                                                                                                                      >  1:79963/1‑141 (MQ=255)
    gttcAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTGTCGAGTTGGACAATGCCGTAGCGTGAGCAAAGTTTTTACAATACATAGt                                                                                                                                    >  2:491792/4‑141 (MQ=255)
     tGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATAGATCTGGAGCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGtt                                                                                                                                   >  1:601542/1‑141 (MQ=255)
      gCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTTTATCGATCAATTTCTATAGAGTTGGACAATGCCGAAGCGTGAGCAGAGTTTTTACCATACATGGTTg                                                                                                                                  >  1:223646/1‑141 (MQ=255)
      gCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTg                                                                                                                                  >  1:558300/1‑141 (MQ=255)
      gCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGGGAAATATTGAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTg                                                                                                                                  >  1:491394/1‑141 (MQ=255)
        aacaataGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGACTAAATGGACGAAACCTTATATCGATCAATCTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCa                                                                                                                                <  2:435260/138‑1 (MQ=255)
           aataGATGAACAAGGTGGGCCACTTAGCGAACAAGTGAATCGTCAAATGATTTAATGTGCTAAACTTTATATCGATCAATTTCTATCGACTTGGACAATTCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCAttt                                                                                                                             <  1:684179/141‑1 (MQ=255)
           aataGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCAttt                                                                                                                             >  1:335539/1‑141 (MQ=255)
           aataGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCAttt                                                                                                                             >  1:475235/1‑141 (MQ=255)
           aataGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCAttt                                                                                                                             >  2:309916/1‑141 (MQ=255)
              agagagagacggTTAGGCACTTAGCCAAGAAGTGAATCGTCAAATGATGAAATTGGCGAAACTTTATATCGCTCAATTTCTATCGAGTTGGACAATGCAGAAGCGTGAGCAACGTTTCTACCATACATGGTTCCATTTAgc                                                                                                                          <  2:20724/131‑1 (MQ=255)
               gATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAgcg                                                                                                                         >  1:156357/1‑141 (MQ=255)
                aTGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAgcgc                                                                                                                        <  1:679255/141‑1 (MQ=255)
                 tGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGAGCa                                                                                                                       >  1:409666/1‑141 (MQ=255)
                   aaCAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTGTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATAAATGGGTGCATTTAGCGCAAc                                                                                                                     >  1:163780/1‑141 (MQ=255)
                    aCAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACa                                                                                                                    <  2:132625/141‑1 (MQ=255)
                     cAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACAt                                                                                                                   <  1:510459/141‑1 (MQ=255)
                      aaGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGATTTTACAACAAATGGTTGCATTTAGCGCAACAta                                                                                                                  >  2:366182/1‑140 (MQ=255)
                      aaGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAAAGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCCACATg                                                                                                                  >  2:38440/1‑141 (MQ=255)
                      aaGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAAGGGACGAAACTTTATATCGAACAATTTCTATCGAGTTGGACAATGCCGAAGCGGGAGCAAAGTTTTTACCATACATGGTTGCATTTCGCGCCACAta                                                                                                                  >  2:34053/1‑140 (MQ=255)
                       gggTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGa                                                                                                                 <  2:479260/140‑1 (MQ=255)
                       aGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGa                                                                                                                 <  1:377623/141‑1 (MQ=255)
                         gTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAAAATGAca                                                                                                               >  1:138366/1‑140 (MQ=255)
                          ggAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCa                                                                                                              <  2:539876/140‑1 (MQ=255)
                           gAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCAt                                                                                                             >  1:70867/1‑141 (MQ=255)
                              ccACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGt                                                                                                          <  1:104831/141‑1 (MQ=255)
                              ccACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGt                                                                                                          <  1:259120/141‑1 (MQ=255)
                              ccACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGt                                                                                                          >  1:319563/1‑141 (MQ=255)
                               cACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGtt                                                                                                         <  1:48870/141‑1 (MQ=255)
                                    aGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACt                                                                                                    <  1:662261/141‑1 (MQ=255)
                                     gCGAACAAGGGAAGCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTa                                                                                                   <  1:379505/141‑1 (MQ=255)
                                           aaGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATCTAGCGCAACATGACCATAGTTTTACTAAAGcac                                                                                             >  2:432650/1‑141 (MQ=255)
                                             gTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAg                                                                                           <  1:517405/141‑1 (MQ=255)
                                               gAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgcg                                                                                         >  1:604176/1‑141 (MQ=255)
                                               gAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTAATAAAGcacgacg                                                                                         >  1:232484/1‑137 (MQ=255)
                                                aaTCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgcgc                                                                                        <  2:133615/141‑1 (MQ=255)
                                                aaTCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAATTTTACTAAAGCACAgcgc                                                                                        >  2:89923/1‑141 (MQ=255)
                                                 aTCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACACGACCATAGTTTTACTAAAGCACAGCGcc                                                                                       >  2:139892/1‑141 (MQ=255)
                                                       aaaTGATTAAATGGACGAAACTTTATATCGATCAATTTCCATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTGACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGa                                                                                 <  1:214096/141‑1 (MQ=255)
                                                           gATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTaa                                                                             >  1:158826/1‑141 (MQ=255)
                                                           gATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGGGATTaa                                                                             >  1:584744/1‑141 (MQ=255)
                                                             ttAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTAGGCAATGCCGAAGCGTGAGCAACGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCAAAgcacataaag                                                                           >  2:380620/1‑132 (MQ=255)
                                                              tAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAgg                                                                          >  2:30782/1‑141 (MQ=255)
                                                                 aTGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGAAAAGTGTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCtt                                                                       <  1:368511/141‑1 (MQ=255)
                                                                     aCGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTAccc                                                                   <  2:428699/141‑1 (MQ=255)
                                                                      cGAAACTTTATAACGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACCGGACCATAGTTTTACTAAAGCACAGCGGCAAGTGATTAAAGGATTACCCa                                                                  >  2:701470/1‑141 (MQ=255)
                                                                             ttATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATcc                                                           <  1:20840/141‑1 (MQ=255)
                                                                              tataTCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCt                                                          <  2:661899/141‑1 (MQ=255)
                                                                               atatCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTg                                                         <  2:677564/141‑1 (MQ=255)
                                                                                tatCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGa                                                        >  1:280214/1‑141 (MQ=255)
                                                                                tatCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGa                                                        <  1:727528/141‑1 (MQ=255)
                                                                                  tCGATCAATTTCTAGCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGaaa                                                      <  1:600116/141‑1 (MQ=255)
                                                                                       cAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACg                                                 <  2:247558/141‑1 (MQ=255)
                                                                                        aaTTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGa                                                >  1:65011/1‑141 (MQ=255)
                                                                                        aaTTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGa                                                >  2:620870/1‑141 (MQ=255)
                                                                                             cTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGCAATGACGATagag                                           >  1:425365/1‑141 (MQ=255)
                                                                                              tATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGt                                          <  2:349044/141‑1 (MQ=255)
                                                                                              tATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGt                                          <  2:110280/141‑1 (MQ=255)
                                                                                               aTCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTc                                         <  1:200093/141‑1 (MQ=255)
                                                                                                tCGAGTTGGACAATGCCGAAGCGTGAGCAAAGGTTTTACCATACACGGTTGCATTTAGCGCAACATGACCTTAGTCTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGAACCTGAAACGACGATAGAGTCa                                        <  2:110062/141‑1 (MQ=255)
                                                                                                 cGAGTTGGACAATGCCGAAGCGTGAGCAAATTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAg                                       <  2:483007/141‑1 (MQ=255)
                                                                                                  gAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGCGCCAGt                                      <  2:475235/141‑1 (MQ=255)
                                                                                                  gAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGt                                      <  2:335539/141‑1 (MQ=255)
                                                                                                   aGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTa                                     >  2:515306/1‑141 (MQ=255)
                                                                                                    gTTGGACAATTCCGAAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGAGCCAAGTGATTAAAGGCTTACCCAATGATCCTGGTATGACGATAGAGTCAGTAt                                    <  1:503579/141‑1 (MQ=255)
                                                                                                     ttGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGCCCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAAGGTTCCTGAAAAAACGTTAAAGTCacaatt                                   >  1:282731/1‑136 (MQ=255)
                                                                                                        gACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAAc                                <  2:604176/141‑1 (MQ=255)
                                                                                                         aCAATGCCGTAGCGTGAGCAAAGCTTTTAACATACATGGTGGAATTTAGCGCAACATGAACATAGTTATACTAAAGCACAGCACCAAGGGATTAAAGGCTTAACCAATGATCCTGAAATGACGATAGAGACAGTATTAACt                               <  2:41554/141‑1 (MQ=255)
                                                                                                         aCAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTACGAATTaaat                               >  1:255962/1‑139 (MQ=255)
                                                                                                           aaTGCCGAAGCTTGAGCAAACGTTTCACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGTAATGACGATAGAGTCAGTATTAACTCa                             <  1:373471/141‑1 (MQ=255)
                                                                                                           aaTGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCa                             <  1:381014/141‑1 (MQ=255)
                                                                                                              gCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAttt                          >  2:581446/1‑141 (MQ=255)
                                                                                                              gCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAttt                          >  1:695788/1‑141 (MQ=255)
                                                                                                               ccGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAtttt                         <  1:30782/141‑1 (MQ=255)
                                                                                                                cGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAttttt                        >  2:138286/1‑141 (MQ=255)
                                                                                                                  aaGCGTGAGCAAAGCTTTTACCATACATGGTTGCATTTAGCGCAACATGAACATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCa                      <  2:103607/141‑1 (MQ=255)
                                                                                                                    gCGTGAGCAAAGTTTTTACCATAGATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCATGAAATGACGATAGGGTCAGTATTAACTCCTTTTTCAAt                    >  1:359575/1‑141 (MQ=255)
                                                                                                                    gCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAAt                    >  1:267326/1‑141 (MQ=255)
                                                                                                                         aGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATc               >  1:165515/1‑141 (MQ=255)
                                                                                                                          tgaaaTTTTTTTCCAGACATGGTTGCATTTGGCGCAACATGCTCATAGTTGTAGTAGAGCACGGCGCAAAGTGATTAAAGGTTTAGCCAATCATGCTGAAATGACGATAGAGTCAGTATTGACTCTTTTTTCGATAGATCa              <  2:365399/139‑1 (MQ=255)
                                                                                                                               gTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAAAAGATCAGGAAg         >  1:16319/1‑141 (MQ=255)
                                                                                                                               gTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCGTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGAACAGGaaa         >  1:144982/1‑140 (MQ=255)
                                                                                                                                 ttttACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGAc       <  2:122489/141‑1 (MQ=255)
                                                                                                                                 ttttACCATACAAGATTGCATTTAGCGCAACATCACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATGTTTCAATAGATCAGGAAGAc       <  2:750239/141‑1 (MQ=255)
                                                                                                                                 ttgtatcatacatgGTTGCATTTAGAGCAACATGACCATAGTTGTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGAc       <  2:1716/135‑1 (MQ=255)
                                                                                                                                 ttgtACCAGACATGGTTGCATGTAGCGGAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGAc       <  1:607895/138‑1 (MQ=255)
                                                                                                                                   ttACCATACATGGTTGCATTTAGGGCACCATGACCATCGTTTTACTAAAGCACAGCGCCCAGTGATTAAAGGCTTACCCAATGATCCTCAAATGACGATAGAGACAGAATTAAGTCATTTTTAAATATATTAGTAAGatca     >  2:428177/1‑138 (MQ=255)
                                                                                                                                   ttACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTa     >  2:638427/1‑141 (MQ=255)
                                                                                                                                     aCCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTAcc   >  2:201812/1‑141 (MQ=255)
                                                                                                                                      ccATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGACCAGGAAGACAACCa  >  2:417979/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                        
AGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTACCA  >  CP000730/479415‑479689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: