Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 707,879 A→T 100% L11I (TTA→ATA)  USA300HOU_0653 ← ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730707,8790AT98.5% 56.7 / ‑6.5 21L11I (TTA→ATA) USA300HOU_0653ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (9/11);  minor base C (0/1);  total (9/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACCGGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGA  >  CP000730/707742‑708009
                                                                                                                                         |                                                                                                                                  
aCCGGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTAGTGGCGAAAAATAAGCTTTACGGACATGTTTTTCATTACATGCTTATTACCTataa                                                                                                                                  >  1:137140/1‑140 (MQ=255)
       gaaTTATATTGACCAATTAAAGTCTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATAACAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGAtt                                                                                                                           <  2:230298/138‑1 (MQ=255)
                           aggcTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACa                                                                                                       <  2:88832/137‑1 (MQ=255)
                                         aaGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATAcctc                                                                                         <  1:165260/140‑1 (MQ=255)
                                                aCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCCCCTCAtca                                                                                  >  2:99962/1‑138 (MQ=255)
                                                        cATTCGGGCCCATGATACCAATTATTACGCTGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGtttt                                                                          >  2:219904/1‑139 (MQ=255)
                                                               gCCCATTATATCAATTATTTCGCCGCTTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAAc                                                                   <  1:109670/140‑1 (MQ=255)
                                                                   aTGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaa                                                               <  1:106871/140‑1 (MQ=255)
                                                                       tACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaattaa                                                           >  2:12893/1‑140 (MQ=255)
                                                                                   cgccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATaaa                                               >  2:141980/1‑140 (MQ=255)
                                                                                        cggaCTGTTATCGATAAGTAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTgag                                          <  1:216019/137‑1 (MQ=255)
                                                                                          tACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAGAAGTTTATGCTAACCTAATTAATTGTTTAATAAACTGATaa                                        >  1:271179/1‑140 (MQ=255)
                                                                                           aCTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAat                                       <  2:22384/140‑1 (MQ=255)
                                                                                                 ttctaTAAGGAAATGTTTTTAAGTACATGCTTATTCCCTATCAACATTTTGAAAGCTTTTGCTTCTAAAAACCCCTCATACCGCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGCATAATAAACTGAGAATATTTAt                                 <  2:72673/136‑1 (MQ=255)
                                                                                                        aGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAACTCTTTTGTTTCTAACAAACGTTTATATCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCa                          <  1:15457/140‑1 (MQ=255)
                                                                                                          gAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGACAAg                        >  1:275225/1‑140 (MQ=255)
                                                                                                              tGTTTTTAAGTACCTGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaaa                    <  2:261617/140‑1 (MQ=255)
                                                                                                              tGTTTTTAAGTACATGCTTATTACCTACGAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaaa                    <  2:60231/140‑1 (MQ=255)
                                                                                                               gTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTGTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATATATCATCTAAAGTaaga                   >  1:142790/1‑138 (MQ=255)
                                                                                                                   ttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGt               <  1:9526/140‑1 (MQ=255)
                                                                                                                                tattaCCTATAAACAGATTTAAATCTTTTGTTTCTATCAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGa  >  1:201381/1‑140 (MQ=255)
                                                                                                                                tattaCCTATAAACAGATTTAAATCTTTTGTTTCTAACAACCGTTTATACCTCCTAATTCAAATTTTAGTCTAACCTAATTAGTTGTATAATAAACTGAGAATAGTTAACATGTCAGATAAATACGTGGTACAAGATAGa  >  1:4914/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                  
ACCGGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGA  >  CP000730/707742‑708009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: