| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,010,363 | C→T | 100% | C146Y (TGT→TAT) | USA300HOU_1861 ← | ABC superfamily ATP binding cassette transporter, membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,010,363 | 0 | C | T | 100.0% | 55.2 / NA | 20 | C146Y (TGT→TAT) | USA300HOU_1861 | ABC superfamily ATP binding cassette transporter, membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (9/11); total (9/11) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTT > CP000730/2010227‑2010486 | ttCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAAt < 2:285559/140‑1 (MQ=255) tgtCAATTTTCTTAAACTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACACCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAAcc < 2:155960/140‑1 (MQ=255) ttCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTc < 1:194228/140‑1 (MQ=255) ttAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCAtt > 2:85575/1‑140 (MQ=255) aaGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTACAATAATAAATGTTATATAATCTAACCAAATATTCATTAACCCGGTTaa > 1:217899/1‑140 (MQ=255) cacCGTTAAAATGGTAAGTGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCtt < 2:45277/140‑1 (MQ=255) atgtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTc < 2:262175/140‑1 (MQ=255) gtaaaatgGGAAGATAAAAAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGATATATAATCTAACCAAATATTCATTATCCCGGTTAAAATGAAATCTTTTGTTTGTTCaa < 2:32868/140‑1 (MQ=255) atgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatca < 2:223579/140‑1 (MQ=255) gATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAAACAAATATTCATTAACACGGTTAAAATGAAATATTTTGTTTGTTCAACATCATTAATCa > 1:240230/1‑140 (MQ=255) tAAAGTCATGTTCACATCTAAAAAAAACATTTTCGAGAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACtt < 1:53751/140‑1 (MQ=255) aaTTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTAc < 2:283972/140‑1 (MQ=255) ttCACATCAAAATAGGACAGTATGGATAGTGCAATAATAATTGTTATATTATCTTACCAAATATTCATTAACCCGGCTAAAATGAATTCTTTGGTTTGTTTGTCATCATTAATCACTCTAGATATTACTTGACCTACTTg < 2:167036/140‑1 (MQ=255) tCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGa > 1:33075/1‑140 (MQ=255) tAAAAAGAACATTATGGATAGTGCAATACTAATTGATATCTAATCTAACCAAATATTCATAAACCCGGTTAAAATGAAATATTTTGTTTGTTCAAAATCATTAATCACTCTAGATATTACTTGACCACGTTGATTATAAg > 2:2276/1‑140 (MQ=255) aCATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCATATGTTTGTACAACATCATTAATCACACTAGATATTACTATACCTACTGGAGTATTAGCATAAAAt > 1:96885/1‑140 (MQ=255) cATTAGGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAAtc < 2:64199/140‑1 (MQ=255) tATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGCCCTCCTTGATTATTAGCATAAAAtctcc > 2:209572/1‑139 (MQ=255) aGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTaa > 1:271779/1‑140 (MQ=255) aataataatTGTTATATAATCTAACCAAATATTCATTAACACGTACAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGAAATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCtt > 1:153531/1‑140 (MQ=255) | TTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTT > CP000730/2010227‑2010486 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |