Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_1861 ← ABC superfamily ATP binding cassette transporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,010,3630CT100.0% 55.2 / NA 20C146Y (TGT→TAT) USA300HOU_1861ABC superfamily ATP binding cassette transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/11);  total (9/11)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTT  >  CP000730/2010227‑2010486
                                                                                                                                        |                                                                                                                           
ttCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAAt                                                                                                                          <  2:285559/140‑1 (MQ=255)
      tgtCAATTTTCTTAAACTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACACCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAAcc                                                                                                                    <  2:155960/140‑1 (MQ=255)
              ttCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTc                                                                                                            <  1:194228/140‑1 (MQ=255)
                 ttAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCAtt                                                                                                         >  2:85575/1‑140 (MQ=255)
                            aaGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTACAATAATAAATGTTATATAATCTAACCAAATATTCATTAACCCGGTTaa                                                                                              >  1:217899/1‑140 (MQ=255)
                                       cacCGTTAAAATGGTAAGTGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCtt                                                                                   <  2:45277/140‑1 (MQ=255)
                                                 atgtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTc                                                                         <  2:262175/140‑1 (MQ=255)
                                                   gtaaaatgGGAAGATAAAAAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGATATATAATCTAACCAAATATTCATTATCCCGGTTAAAATGAAATCTTTTGTTTGTTCaa                                                                       <  2:32868/140‑1 (MQ=255)
                                                        atgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatca                                                                  <  2:223579/140‑1 (MQ=255)
                                                               gATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAAACAAATATTCATTAACACGGTTAAAATGAAATATTTTGTTTGTTCAACATCATTAATCa                                                           >  1:240230/1‑140 (MQ=255)
                                                                              tAAAGTCATGTTCACATCTAAAAAAAACATTTTCGAGAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACtt                                            <  1:53751/140‑1 (MQ=255)
                                                                                     aaTTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTAc                                     <  2:283972/140‑1 (MQ=255)
                                                                                        ttCACATCAAAATAGGACAGTATGGATAGTGCAATAATAATTGTTATATTATCTTACCAAATATTCATTAACCCGGCTAAAATGAATTCTTTGGTTTGTTTGTCATCATTAATCACTCTAGATATTACTTGACCTACTTg                                  <  2:167036/140‑1 (MQ=255)
                                                                                         tCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGa                                 >  1:33075/1‑140 (MQ=255)
                                                                                                tAAAAAGAACATTATGGATAGTGCAATACTAATTGATATCTAATCTAACCAAATATTCATAAACCCGGTTAAAATGAAATATTTTGTTTGTTCAAAATCATTAATCACTCTAGATATTACTTGACCACGTTGATTATAAg                          >  2:2276/1‑140 (MQ=255)
                                                                                                        aCATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCATATGTTTGTACAACATCATTAATCACACTAGATATTACTATACCTACTGGAGTATTAGCATAAAAt                  >  1:96885/1‑140 (MQ=255)
                                                                                                         cATTAGGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAAtc                 <  2:64199/140‑1 (MQ=255)
                                                                                                            tATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGCCCTCCTTGATTATTAGCATAAAAtctcc              >  2:209572/1‑139 (MQ=255)
                                                                                                                   aGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTaa       >  1:271779/1‑140 (MQ=255)
                                                                                                                        aataataatTGTTATATAATCTAACCAAATATTCATTAACACGTACAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGAAATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCtt  >  1:153531/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                           
TTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTT  >  CP000730/2010227‑2010486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: