Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,360,799 A→G 100% G66G (GGT→GGC adk ← adenylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,360,7990AG97.3% 79.6 / ‑6.2 29G66G (GGT→GGCadkadenylate kinase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base G (16/10);  minor base T (2/0);  total (19/10)
Fisher's exact test for biased strand distribution p-value = 5.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GATGACAGCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATACCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTC  >  CP000730/2360664‑2360931
                                                                                                                                       |                                                                                                                                    
gatgaCAGCATCAATGTTTCTGTCAAGCTCAGAAATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGGTGCCTa                                                                                                                                  >  2:152335/1‑140 (MQ=255)
          tCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTATTGAATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTtc                                                                                                                        >  1:80525/1‑140 (MQ=255)
                tttCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAgg                                                                                                                  <  1:167143/140‑1 (MQ=255)
                 ttttGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGa                                                                                                                 <  1:162804/137‑1 (MQ=255)
                       aaaGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAAt                                                                                                           <  1:278504/139‑1 (MQ=255)
                         aGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTc                                                                                                         <  1:186953/140‑1 (MQ=255)
                             gaGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGGAATTCTTTCCTTCACGATGCCTACCGTCACTTCATCCGGACCTAATTCACCa                                                                                                     >  2:170178/2‑140 (MQ=255)
                                       attattTAATTCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCatat                                                                                           <  2:87487/140‑1 (MQ=255)
                                               aTGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTTTTTTTTTTTCAGAAATTATTTCCTTAACGATTTCGGCAATCGTTTCATCAGGTACTAATTTGGCACGGTCCATTTaatttttt                                                                                   >  2:134805/1‑134 (MQ=255)
                                                tGCCTCAGCTTGCTCGATTGTGCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAg                                                                                  >  1:149494/1‑140 (MQ=255)
                                                        cTTGCTCGATTGTTCTTGGGAGGCCAACTAATAAAAAGCCTTTTTTTGCATCGTCTTCATAAATTCTTTCCTTAAGGATACCTCCAGTCACTTCATCAGGAACTAATTCGCCAAGGCCCATATAAGACGTAGCTTCTTTa                                                                          >  2:170291/1‑140 (MQ=255)
                                                           gCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCt                                                                       >  1:111494/1‑140 (MQ=255)
                                                            cTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTa                                                                      <  1:234585/140‑1 (MQ=255)
                                                                      cTTGAGAAGCCATCTAATAAAAAGCCTTTTTTTGCATAGTCTTAAGAAATTCTTTCCTTAACGATTCCTACAGTCACTTCATCAGGAACTCATTCGTCGCGGGACATATAAGACTTAACTTCTTAACATAATTCAGTttc                                                            >  2:11876/1‑140 (MQ=255)
                                                                                cgtCTAATAAAAAGCCTATTTTTTCATCGTCTTCAGAAAGTCTTTCCTGAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTtata                                                  >  2:26561/3‑140 (MQ=255)
                                                                                  tCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGc                                                <  2:80525/140‑1 (MQ=255)
                                                                                  tCTAATAAAAAGCCTTTTTTTGCATAGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTAACTTCATCAGGAACTAATTCGACACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGc                                                >  2:98819/1‑140 (MQ=255)
                                                                                                    ttttttttttCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCAcc                              >  2:251537/10‑140 (MQ=255)
                                                                                                         aTCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATTTCCCCAGTTg                         >  1:143570/1‑140 (MQ=255)
                                                                                                         aTCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGAATTAGCTTATTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTTTGAACATGACACCAtttt                         >  1:232193/1‑139 (MQ=255)
                                                                                                                   gAAATTCTTTTCTTAACGATGCCTACATTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTgggg               <  1:121117/140‑1 (MQ=255)
                                                                                                                   gAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCCCGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTAAACATGTCACCAGTTGAACTGTtggg               >  1:56075/1‑140 (MQ=255)
                                                                                                                   gAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGGACATGTCACCAGTTGAAATGTgggg               >  2:282783/1‑140 (MQ=255)
                                                                                                                   gAAATTCGTTCCTTAACGATGCCTACAGTCACTTTAGCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTgggg               <  1:257674/140‑1 (MQ=255)
                                                                                                                        tCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTg          <  2:42184/140‑1 (MQ=255)
                                                                                                                         cTTTCCTTAACGATGACTTCATTTTGTTCATCAGGAACTAATTCGCCACGGGCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTgg         >  2:206708/1‑140 (MQ=255)
                                                                                                                          tttCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTggg        >  2:36974/1‑140 (MQ=255)
                                                                                                                                tAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATTTCACCAGTTGAAATTTGGGGTATTGGGAATTTc  >  2:216763/1‑140 (MQ=255)
                                                                                                                                tAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTc  >  1:189761/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                    
GATGACAGCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATACCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTC  >  CP000730/2360664‑2360931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: