BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,997	T→C	31.3%	T185T (ACA→ACG)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,997	0	T	C	31.3%	619.0 / inf	552	T185T (ACA→ACG)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (181/196); new base C (89/83); total (272/280)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 553 total aligned reads displayed.

CAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCT  >  CP000730/35867‑36136

cAGCGTTCGTTTAACTTGCGAGCATGATGCTCATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                                                                                   <  1:76648/141‑1 (MQ=11)

 aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt                                                                                                                                  >  1:103933/1‑141 (MQ=11)

   tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCATTTTATCCGAAGCTTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                                                                                                                >  1:277676/1‑141 (MQ=11)

    gtctgtgtAACTTGCTTGCATGATGTTAGTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                                                                                                               <  2:145194/134‑1 (MQ=11)

    gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg                                                                                                                               >  1:238530/1‑141 (MQ=255)

     ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                                                                              <  2:216593/141‑1 (MQ=32)

     ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAGTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                                                                              >  1:41949/1‑141 (MQ=17)

     ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTgt                                                                                                                              >  1:363729/1‑140 (MQ=17)

     ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                                                                              <  2:17873/141‑1 (MQ=17)

        gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                                                           <  2:235275/141‑1 (MQ=17)

           tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCtt                                                                                                                        <  1:335439/141‑1 (MQ=11)

            aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGTGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTg                                                                                                                       <  2:343035/141‑1 (MQ=11)

             aCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTATATCTGAAGCGACCTACGGTTCTTTTTATATATAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGTTATCTTGt                                                                                                                      <  2:281808/141‑1 (MQ=11)

             aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGACAACTTCGCTGTATTGATACTTTGATACCTTGt                                                                                                                      <  1:91341/141‑1 (MQ=11)

               ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                                                                                    <  1:6287/141‑1 (MQ=17)

                tgttaacATAATGCTAATTTCTTGGCATGGGAAAAATCCTTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctt                                                                                                                   <  1:281987/135‑1 (MQ=11)

                tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                                                                                   <  2:53700/141‑1 (MQ=17)

                 gCTAGCATGATGCTAATTTCGTGGCATGGCGGATTGCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                                                  >  1:378593/1‑141 (MQ=11)

                     gCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGCTCCCCTGATATCTTGtctttcttc                                                                                                              >  2:95440/1‑140 (MQ=11)

                     gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTGTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                                                                              >  1:294514/1‑141 (MQ=17)

                       atgatgCTAATTTCGTGGCATTGTGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACt                                                                                                            <  2:363183/141‑1 (MQ=11)

                        tgatgCTAATTTCGCGGCATGACGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                                                                                                           >  1:199249/1‑141 (MQ=25)

                         gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                                                          <  1:242723/141‑1 (MQ=25)

                         gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCCGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                                                          <  2:87911/141‑1 (MQ=21)

                            gCTAATTTCGTGGCATGGGGAAAATCCTGAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAat                                                                                                       <  1:179682/141‑1 (MQ=11)

                               aaTTTCGTGGCATGGCGCAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGACCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTCTTGATACTTCGCTACCTTGTCTCTCTTACTTTatgctg                                                                                                    >  2:303408/1‑136 (MQ=11)

                               aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatata                                                                                                    >  2:131051/1‑140 (MQ=21)

                               aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                                                    <  2:172646/141‑1 (MQ=25)

                               aaTGTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATg                                                                                                    <  2:140738/141‑1 (MQ=11)

                                aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGa                                                                                                   >  2:313920/1‑141 (MQ=11)

                                    cctGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAAAGAGCGTACATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                                               <  1:165889/139‑1 (MQ=255)

                                     gTGGCTTGGCGAAAGTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                              <  2:107997/141‑1 (MQ=255)

                                     gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                              <  1:343484/141‑1 (MQ=255)

                                      tGGCATTGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTTCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                                                                             <  2:26261/141‑1 (MQ=255)

                                      tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTACTCACTTTAATATGACGgaga                                                                                             >  1:381863/1‑141 (MQ=255)

                                        gcatgttcaaatgccGTAGGTCTGAAGGCCTCTGTGGTTCTTTTTATATGGAGCTTAAATACACTCAATACCTTTTTAAGTATTCTTTGCTGTATTGATACTTTGATCCCTTGTCTTTCTGACTTTAATATGACGGTGATc                                                                                           <  2:353579/128‑1 (MQ=11)

                                          agggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTATTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                                                                         <  2:2429/139‑1 (MQ=255)

                                           tggtgtaaaTCCGTAGCTCAGAAGAGATCTACGGTTCCTTGTATAGAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTCCTTTAATATGACGGTGATCTTg                                                                                        <  2:294121/135‑1 (MQ=14)

                                           tGGCGAACATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                                                        <  1:268533/141‑1 (MQ=255)

                                            ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGGTCTTGc                                                                                       >  1:6593/1‑141 (MQ=255)

                                            ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                                                       <  2:277761/141‑1 (MQ=255)

                                            ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                                                       <  2:159604/141‑1 (MQ=255)

                                              ggAAACTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                                                                                     >  1:160995/2‑141 (MQ=255)

                                                   aTCCGTAGATCTGAAGAGACCTGCGGGTCTTGTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATgc                                                                                >  2:320121/1‑140 (MQ=255)

                                                     ccGTAGATCTGAATATACCTTCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                              <  2:15301/141‑1 (MQ=255)

                                                     ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                              >  1:128268/1‑141 (MQ=38)

                                                     ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                              >  1:255664/1‑141 (MQ=37)

                                                       gTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCATAAATACATTCAATACCGTTTACAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTTCTCCATGAgggt                                                                            >  2:202608/1‑139 (MQ=255)

                                                        tAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                                                                           <  1:140256/141‑1 (MQ=255)

                                                         aGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAt                                                                          <  1:101319/141‑1 (MQ=255)

                                                           aTCTGATGACACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                                                                        <  2:381130/141‑1 (MQ=255)

                                                            tCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCa                                                                       >  1:340469/1‑141 (MQ=38)

                                                             cttaaGAGACTGGCGGGTCTTGTTATAGAGAGCGTAAATACACTCAATACCGTTTAAAGTATGCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTGCGAGGACGGTGATCTTGCTCAATGAGGTTATTCAg                                                                      <  1:14749/138‑1 (MQ=11)

                                                                    gaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTc                                                               <  2:115811/141‑1 (MQ=255)

                                                                        tGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTCGCCGTCTTTAGACTGTGGTACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATg                                                           >  2:61433/1‑141 (MQ=11)

                                                                          cGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTGTTGCTTACTTTAAAGTGACGGCGCCCTTGCTCCCTGAGGTTCTTGAGATATTTCGATGTa                                                         >  2:326325/1‑141 (MQ=11)

                                                                                    tatataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGt                                               <  1:170088/141‑1 (MQ=255)

                                                                                    tacataGAGCTTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTCATATGACTGAGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGt                                               <  2:306809/138‑1 (MQ=11)

                                                                                     atataGAGCGTAAATACACCCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTc                                              <  1:290700/141‑1 (MQ=255)

                                                                                        taGATCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAgg                                           >  1:259842/1‑141 (MQ=255)

                                                                                        taGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAgg                                           <  1:80854/141‑1 (MQ=255)

                                                                                          gagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtt                                         >  1:28507/1‑141 (MQ=255)

                                                                                            gCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgttta                                       >  1:305927/1‑141 (MQ=255)

                                                                                              gTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGTCAGTCAGgtttaag                                     <  1:249844/141‑1 (MQ=255)

                                                                                                aaaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtt                                   <  1:313920/141‑1 (MQ=255)

                                                                                                aaaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtt                                   <  1:21814/141‑1 (MQ=255)

                                                                                                 aaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                  >  1:111820/1‑141 (MQ=255)

                                                                                                 aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                  >  1:12052/1‑141 (MQ=255)

                                                                                                 aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                  >  1:223562/1‑141 (MQ=255)

                                                                                                 aaTACAATCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTCATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                  <  1:34954/141‑1 (MQ=255)

                                                                                                  aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGTTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACTGTCTGgtttaagttta                                 >  1:174520/1‑141 (MQ=14)

                                                                                                  aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTAAGgtttaatttta                                 >  2:45907/1‑141 (MQ=255)

                                                                                                  aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                 <  2:186367/141‑1 (MQ=255)

                                                                                                   tACATTCAATACCTTTTAAGGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                                >  1:228553/1‑141 (MQ=255)

                                                                                                   tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                                >  2:270525/1‑141 (MQ=255)

                                                                                                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGGTACTTTGATACCTGGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATGTTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                >  2:348543/1‑141 (MQ=14)

                                                                                                    aCATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCACGTTTAAGTTTaaa                               <  2:223801/141‑1 (MQ=255)

                                                                                                       ttCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGc                            <  2:357674/141‑1 (MQ=255)

                                                                                                       ctCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGGGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGGTTAAGTTTAAAAGc                            >  1:20569/2‑141 (MQ=255)

                                                                                                       ctCAATACCTTTTAAAGTATTCTATGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCACTGCGATTATTCTGATATTTCGATGTACAATGTCAGTCAGGTTGAAGTTTAGAAGc                            >  2:285894/2‑141 (MQ=14)

                                                                                                       cgcAATCCCTTTTAAAGTATACTTTGCTGTATTGATACTTAGATACCTTGCCATTCTTACTTTAATATGACGGTTATCTTGCTCAATGAGTTTCTTCAGATATTTCGATGTACAATGGCCGTCAACGTCAAGTTTaaaatc                            >  2:331728/3‑139 (MQ=14)

                                                                                                        tCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCt                           <  1:99318/141‑1 (MQ=255)

                                                                                                         cAAGACCTTTTAGAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCtt                          <  2:72633/141‑1 (MQ=255)

                                                                                                           aTACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTa                        <  1:49538/141‑1 (MQ=255)

                                                                                                             aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                      <  1:131730/141‑1 (MQ=255)

                                                                                                             aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTAGATGTAAAAAGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                      >  1:6010/1‑141 (MQ=255)

                                                                                                                 tttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTTTTCAGATATTTCGATGTACAATGGCAGTCAGGTGTAAGTTTAAAAGCTTTAATTACt                  >  2:249465/1‑141 (MQ=255)

                                                                                                                   tAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGCCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACttt                >  1:326534/1‑141 (MQ=255)

                                                                                                                    aaaGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTa               >  2:299930/1‑141 (MQ=255)

                                                                                                                      aGTATTCTTTGCCGTATTGATAATTTGATATCTTGTCTTTCGTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGc             <  2:208766/141‑1 (MQ=11)

                                                                                                                       gTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAAcc            >  1:272683/1‑141 (MQ=255)

                                                                                                                       gTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGcc            >  1:191876/1‑141 (MQ=255)

                                                                                                                         aTTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGATAGTCAGGTTTAAGTTTAAAAGCTTTAATAATTTTAGCCAt          >  2:194765/1‑141 (MQ=14)

                                                                                                                           tCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTg        >  1:318472/1‑141 (MQ=255)

                                                                                                                            ttttGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGc       <  2:77961/140‑1 (MQ=255)

                                                                                                                              ttGCTGTATTGGTAGTTTGATGTCTTGTCTTTCTTAGTTTGATATGACTGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCTGTCTGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTa     <  2:134560/141‑1 (MQ=11)

                                                                                                                               tgctgcATTGATACTTTTATTCCTTGCCTTTCGTACTTTAATAAGTCGGTGTTTTTGCTCAACGAGGTTATTCAGATATCTCGATTTACAATGCCAGTCAGGTTTAAGTTTAAAAGCTTTCATTTCTCTAACCATAGCTAc    <  2:10495/141‑1 (MQ=9)

                                                                                                                                tcTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCATGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc   <  1:121967/140‑1 (MQ=255)

                                                                                                                                gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc   <  1:331802/141‑1 (MQ=255)

                                                                                                                                 cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt  <  2:12052/141‑1 (MQ=255)

CAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCT  >  CP000730/35867‑36136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`