BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,174	T→C	31.0%	K126K (AAA→AAG)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,174	0	T	C	31.0%	378.7 / inf	452	K126K (AAA→AAG)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (141/168); new base C (75/63); total (220/232)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-02

Only 100 of 461 total aligned reads displayed.

ATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAT  >  CP000730/36034‑36314

   tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGGGGcttc                                                                                                                                           >  1:303417/1‑140 (MQ=11)

    gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                                                                                                          <  1:3377/141‑1 (MQ=21)

     ccGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                                         <  1:257491/140‑1 (MQ=16)

     aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCCGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGCTGCCTGAACTGTAATTACGTTTCGCGGATTAcc                                                                                                                                         >  2:46234/1‑141 (MQ=11)

      cGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCa                                                                                                                                        >  1:18617/1‑141 (MQ=18)

        gttaTCTTGCTTAATGAGGTTATTCAGATATTTCGATGTACAATGTCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                                      <  2:254915/138‑1 (MQ=255)

        gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                                      <  2:340460/141‑1 (MQ=12)

         tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAt                                                                                                                                     >  2:250325/1‑141 (MQ=255)

         tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAAt                                                                                                                                     >  2:179899/1‑141 (MQ=255)

          gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAtt                                                                                                                                    >  2:27439/1‑141 (MQ=12)

           aTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTTTAATTACCTTTTGAGGTTTACCAAATTg                                                                                                                                   <  2:350413/141‑1 (MQ=12)

            tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTACTTACCTTTTGAGGTTTACCAAATTGt                                                                                                                                  >  1:77085/1‑141 (MQ=12)

            tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                                  >  2:356559/1‑141 (MQ=17)

            tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                                  >  1:224916/1‑141 (MQ=17)

            tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                                  >  1:250388/1‑141 (MQ=255)

             cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGtt                                                                                                                                 >  2:86466/1‑141 (MQ=17)

             cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTAATGCCCTCGTTGAATGTGTCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGtt                                                                                                                                 >  1:11005/1‑141 (MQ=11)

             cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTA‑TTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGttt                                                                                                                                >  2:246763/1‑141 (MQ=11)

               tGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTa                                                                                                                               <  2:318472/141‑1 (MQ=17)

                gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTCACTACCTTTTGAGGCTTACCAAATTGTTTaa                                                                                                                              >  1:59395/1‑141 (MQ=11)

                gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAGATTGTTTaa                                                                                                                              >  2:311920/1‑141 (MQ=11)

                gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACAAAGTTGTTTaa                                                                                                                              >  2:294980/1‑141 (MQ=11)

                 cTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAAt                                                                                                                             >  1:125897/1‑141 (MQ=255)

                  tCAATGGGGTTATTCAGATATTTCGATGGACATTGACAGTCAGTTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCTTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATg                                                                                                                            <  2:79936/141‑1 (MQ=11)

                    aaTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgag                                                                                                                          <  1:198774/141‑1 (MQ=17)

                      tGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgagcc                                                                                                                        >  2:114444/1‑139 (MQ=12)

                      tGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGAc                                                                                                                        >  1:381267/1‑141 (MQ=32)

                      tGAGGTTATACAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGAc                                                                                                                        <  2:215426/141‑1 (MQ=11)

                       gAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTTTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACg                                                                                                                       <  1:167868/141‑1 (MQ=12)

                       gAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACg                                                                                                                       <  2:301885/141‑1 (MQ=17)

                       gAGGTTATTCAGATATTTCGATGGACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGTTTGAGCAAATTGTTTAATGAGACg                                                                                                                       <  2:176341/141‑1 (MQ=11)

                        aGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                                      <  1:34574/141‑1 (MQ=17)

                         ggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                                                                     <  1:88709/141‑1 (MQ=17)

                           ttATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTg                                                                                                                   <  2:67734/141‑1 (MQ=17)

                            tATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                                  >  1:23767/1‑141 (MQ=12)

                                    tatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                                                          <  1:175897/141‑1 (MQ=12)

                                      tttCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCatt                                                                                                        >  2:153037/1‑140 (MQ=12)

                                       ttCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCatat                                                                                                       <  2:199249/141‑1 (MQ=32)

                                           aTGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGCGACGTTTGATAAACGCATATGCTg                                                                                                   >  1:349826/1‑141 (MQ=12)

                                                    gACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTGGATAAACGCATATGCTGAATGATTAt                                                                                          <  2:237827/141‑1 (MQ=11)

                                                                                   tattttAGCCATGGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTCCCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgt                                                           <  1:194827/138‑1 (MQ=1)

                                                                                      tttAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                                        >  2:145793/1‑141 (MQ=2)

                                                                                      tttAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                                        >  1:242697/1‑141 (MQ=11)

                                                                                       ttAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatgt                                                       >  1:267937/1‑141 (MQ=9)

                                                                                        taaccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTc                                                      <  2:284427/138‑1 (MQ=17)

                                                                                         ggCCATTGCTGCCGTCGTTGAAGGGGCCGGAGCTGTAACTACCTTTTGAGGCTCACCAAATGGTTTAATGCGACGTTTGATAACCGCATATGCTGAATGATTATCTCCTTGCTTACGCAACCAAATATCTAATGTATGTcc                                                     <  2:164414/140‑1 (MQ=11)

                                                                                         aaccACTGCTACCTTCGTTGAAGGTGCCTGAACTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                                     <  1:102442/139‑1 (MQ=12)

                                                                                             aTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctct                                                 >  1:308666/1‑141 (MQ=32)

                                                                                             aTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACATTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctct                                                 <  1:144408/141‑1 (MQ=17)

                                                                                              ttGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATTAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTCCGCAACCAAATATCTAATGTATGTcctcctt                                                >  1:341369/1‑136 (MQ=11)

                                                                                               tGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCACACAAATATCTAATGTATGTcacgctca                                               >  2:54425/1‑134 (MQ=255)

                                                                                               tGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCACTCTgt                                               >  2:227129/1‑140 (MQ=17)

                                                                                               tGCCACCTTCGTTGAATGGGCCTGATCTGTAATTACCTTTTGAGGTTTCCCAAATTGTTTAATGAGACGTTTGACAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACTAAATATCTAATGTATGTCCCTTTgt                                               <  2:102309/141‑2 (MQ=11)

                                                                                                gCTGCCTTCGTTGCAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGTTTACGCAACCAAATATCTAATGTATGTCCCTCTgta                                              <  2:63712/141‑3 (MQ=11)

                                                                                                gCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTCCGCTAACAAATATCTAATGTATGTCCCTCTgta                                              <  1:91399/141‑3 (MQ=11)

                                                                                                gCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgta                                              <  1:298528/141‑3 (MQ=255)

                                                                                                gCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGTAACTAAATATCTAATGTATGTCCCTCTgta                                              <  2:271087/141‑3 (MQ=12)

                                                                                                gCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                              <  1:283240/141‑1 (MQ=32)

                                                                                                 cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAt                                             <  2:159625/141‑1 (MQ=32)

                                                                                                 cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAt                                             >  2:374273/1‑141 (MQ=32)

                                                                                                  tgccTTGGTTGAACGTGCCTGATCTGTAATCAATTTTTTAGGCTTACCAAATTGGTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCGCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                                            <  1:32921/139‑1 (MQ=11)

                                                                                                   gccTTCGTTGAAGGTCACTGATCGGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                           >  2:328746/2‑141 (MQ=11)

                                                                                                   aCCTTCGTTGAAGGTGCCTGATGTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATTTAATGTATGTCGCTCTGCATCa                                           >  2:138688/1‑141 (MQ=12)

                                                                                                   aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTATTGTATGTCCCTCTGTATCa                                           >  2:292391/1‑141 (MQ=17)

                                                                                                   aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                           <  2:194748/141‑1 (MQ=17)

                                                                                                   aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                                           >  1:121832/1‑141 (MQ=32)

                                                                                                    ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGTCGTTTGTTACACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATGTCTAATGTATGTCCTTCGGTATCaa                                          >  1:27948/1‑141 (MQ=11)

                                                                                                      ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                                        >  1:205385/1‑141 (MQ=17)

                                                                                                       tCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATgg                                       >  2:198558/1‑141 (MQ=32)

                                                                                                         gTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCa                                     <  2:335405/141‑1 (MQ=32)

                                                                                                          ttGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCAc                                    >  1:154883/1‑141 (MQ=12)

                                                                                                            gAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGTTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGa                                  >  1:72769/1‑141 (MQ=17)

                                                                                                              aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAACTTGTTTACTAACGAGATTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAGCCAATTATCTAATGTATGTCCCTCTGTATCAATGGCACGtta                                >  1:318037/1‑141 (MQ=11)

                                                                                                              aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGata                                >  2:182769/1‑141 (MQ=32)

                                                                                                               ggTTCCTGCTCGGTCAATACCTTTTTAGGTTTACCAAATTTTTTAATGAGACGTTTGATACACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCCATGGCACGatat                               <  2:107130/141‑1 (MQ=11)

                                                                                                               ggTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGatat                               >  2:363716/1‑141 (MQ=32)

                                                                                                                 tGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATaa                             >  2:260364/1‑141 (MQ=12)

                                                                                                                  gcttGATCTGTAATTACCTTTTGAGGTTTACAAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCATTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATaaa                            <  1:234322/138‑1 (MQ=255)

                                                                                                                   ccTGTTCTGTTATTTCCTTTTGAGGTTTACCAAATTGTTTAATGCGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCTCTCTGCATCAATGGCACGATATAAAt                           <  2:9119/141‑1 (MQ=11)

                                                                                                                     tGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGTTTTAAATaa                         >  2:74030/1‑140 (MQ=12)

                                                                                                                     tGATCTGTAATTACCTTTTGAGGAATACCAAATTGTTTAATGAGACGTTTGATAAACACATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAAAGTAAGACCCCCTGTATCAATGGCCCGATATaaaaaa                         >  2:242616/1‑138 (MQ=11)

                                                                                                                      gATTTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGc                        <  2:381568/141‑1 (MQ=17)

                                                                                                                      gATCTGAAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGTCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGc                        <  1:46266/141‑1 (MQ=17)

                                                                                                                       accTGTACTCACCCATTAAGGTTTACCAAATTGTCTAAAGAGACGCTCCATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCt                       <  1:357330/139‑1 (MQ=11)

                                                                                                                         cTGTAATTACCTTTTGAGGTTTACTAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTcc                     <  1:293986/141‑1 (MQ=17)

                                                                                                                          tttaacgaCCTTTTGAGGCTTACCAAATTGTTTAATGTGGCGTTTGATAAACGCCTATGCTGAATGATTATCTCGTTGCTTCCGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCa                    <  1:81990/134‑1 (MQ=11)

                                                                                                                          tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCa                    <  1:262420/141‑1 (MQ=17)

                                                                                                                            tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtt                  >  1:371611/1‑141 (MQ=17)

                                                                                                                                tACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTcc              <  1:219680/141‑1 (MQ=17)

                                                                                                                                 aCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGACACGATATAAATAGCTCCATTTTCCt             >  1:230905/1‑141 (MQ=17)

                                                                                                                                  ccTTGTGAGGCTTACCAAATTGTTTAATGAGAAGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATCTAAATAGCTCCATTTTCCtt            >  1:273484/1‑141 (MQ=11)

                                                                                                                                   cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCttt           <  1:366505/141‑1 (MQ=32)

                                                                                                                                   cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCttt           <  2:308144/141‑1 (MQ=32)

                                                                                                                                   cTTTTGAGGTTTACCAAATTGTTTAATGAGAAGATTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt           <  1:14981/141‑1 (MQ=12)

                                                                                                                                    ttttGCGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCGCCATTTTCCtttt          <  1:52458/141‑1 (MQ=255)

                                                                                                                                       tGATGTTTACAAAATTGTTTAATGAGACTTTTGATAAACGCATATGCTGAATGATTATCTCTTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt       <  2:126536/141‑1 (MQ=255)

                                                                                                                                         aGGTTTACCAAATTGTTTAATGAGACGTTTGATAATCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtttt     <  1:290317/141‑1 (MQ=17)

                                                                                                                                          ggTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATTTTGTATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGGACGATATAAATAGCTCCATTTTCCTTTTAttttt    >  2:221561/1‑140 (MQ=11)

                                                                                                                                          ggTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg    >  1:135269/1‑141 (MQ=32)

                                                                                                                                           gTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGa   <  1:158247/141‑1 (MQ=32)

ATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAT  >  CP000730/36034‑36314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`