BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,315	G→A	61.7%	Y79Y (TAC→TAT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,315	0	G	A	61.7%	299.5 / inf	621	Y79Y (TAC→TAT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (125/109); new base A (202/174); total (336/285)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 623 total aligned reads displayed.

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTT  >  CP000730/36175‑36454

 tACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGt                                                                                                                                            <  1:244954/141‑1 (MQ=32)

  aCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTa                                                                                                                                           <  1:100025/141‑1 (MQ=32)

          gTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGTTCTAAATAGCACCATTTTCCTTTTATTCTGATATACGTCtcat                                                                                                                                   >  1:239250/1‑141 (MQ=11)

           tttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTGCGCAAGCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcatc                                                                                                                                  <  1:296630/141‑1 (MQ=17)

            ttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAAACAACTATCTAATTTAAGTCCCTCTTCATCAATGTCATGATATAAATAGCTTCATTTTCCTTTTATTTTGATGTTCGTCttatca                                                                                                                                 >  2:230764/1‑141 (MQ=11)

                tGACACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                                                             <  1:105003/141‑1 (MQ=255)

                        ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTg                                                                                                                     <  2:118974/141‑1 (MQ=17)

                        ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTg                                                                                                                     >  2:235968/1‑141 (MQ=32)

                          gATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGta                                                                                                                   <  1:335067/141‑1 (MQ=32)

                           aTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaa                                                                                                                  >  2:356118/1‑141 (MQ=17)

                            tAAAAGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATTTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaat                                                                                                                 <  2:329060/141‑1 (MQ=255)

                             aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaata                                                                                                                >  1:208861/1‑141 (MQ=32)

                                  cATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtt                                                                                                           <  2:289015/141‑1 (MQ=255)

                                   atatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCgtt                                                                                                          >  2:262770/1‑141 (MQ=21)

                                    tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCCCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtttt                                                                                                         >  2:57675/1‑141 (MQ=255)

                                        cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAt                                                                                                     >  2:93022/1‑141 (MQ=37)

                                              gATTATCTCGTTGCTTACGCGACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGGACGTCTCATCAATACGCCTTTTGTAATAAGCATTTTTATCCttta                                                                                               >  1:268716/1‑140 (MQ=11)

                                              gATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtttt                                                                                               >  1:348209/1‑141 (MQ=255)

                                              gATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtttt                                                                                               >  1:312089/1‑141 (MQ=39)

                                               aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTATACGTCTCATCAATACGCCATTGGTAATAAGCTTTTTTATgactttc                                                                                              >  1:85542/1‑135 (MQ=14)

                                               aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttttt                                                                                              <  1:131367/141‑1 (MQ=255)

                                               aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAATTGTAATAAGCTTTTTTATGtttttt                                                                                              >  1:120970/1‑141 (MQ=255)

                                               aTTAGCTCGTCGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAAGCCATTTGTAATAAGCTTTTTTATGCttttt                                                                                              <  1:32118/141‑1 (MQ=14)

                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATCTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTATGTCTCAGCCATACGCCATTTGTACTAAGCTTTTTTTTGCTTTttct                                                                                            >  2:181462/1‑141 (MQ=14)

                                                  ttctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAACTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                                                                           <  1:258951/140‑1 (MQ=255)

                                                  aTCTCGTTGCTTACTCAACCAAATATCTAATGTATGTCCCTCTTCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTTTAATAAGCTTTTTTATGCTTTttctt                                                                                           >  1:80381/1‑141 (MQ=255)

                                                  aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                                                                           <  1:62769/141‑1 (MQ=255)

                                                   tctcGTTGTTTACACAACCAAATATCTTATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                          <  2:143219/141‑1 (MQ=14)

                                                   tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                          <  2:149213/141‑1 (MQ=255)

                                                   tctcGTTGCTTACACAACCAAATTTCTATTGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                          <  1:68866/141‑1 (MQ=14)

                                                      cGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCaa                                                                                       >  2:268746/1‑141 (MQ=255)

                                                            ttACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGa                                                                                 >  2:312655/1‑141 (MQ=255)

                                                            ttACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCATGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGa                                                                                 <  2:379513/141‑1 (MQ=255)

                                                              aCGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATa                                                                               <  2:294142/141‑1 (MQ=255)

                                                              aCGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATa                                                                               >  2:209996/1‑141 (MQ=39)

                                                                 cAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATataa                                                                            <  2:12571/141‑4 (MQ=38)

                                                                  aaCCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATACGCTGTTTTATGCTTTTTCTTCCAAATTTGATATaaa                                                                           <  1:340000/141‑1 (MQ=11)

                                                                    ccAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTCCAAATTTGATATAAAAt                                                                         >  1:378348/1‑141 (MQ=11)

                                                                       aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                      >  2:217596/1‑141 (MQ=255)

                                                                         tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTCCAAATTTGATATAAAATTgggg                                                                    >  1:44548/1‑141 (MQ=255)

                                                                         aatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                    <  2:167458/140‑1 (MQ=37)

                                                                           tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                                                                  >  2:173635/1‑141 (MQ=255)

                                                                               atgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                                                              >  2:247860/1‑141 (MQ=255)

                                                                               atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCAATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCATTTTCTTCAAAATTTGATATAAAATTTGGGCatcct                                                              >  2:285013/1‑138 (MQ=11)

                                                                                   atgtCCCCCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                          >  2:48749/1‑141 (MQ=11)

                                                                                    tgtCCGTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa                                                         <  2:277716/141‑1 (MQ=255)

                                                                                     ggcccTCTGTATCAATGGCACGATATTAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa                                                        <  2:113388/139‑1 (MQ=11)

                                                                                      tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTCGGGCATATTCTTGAAc                                                       >  1:304687/1‑141 (MQ=255)

                                                                                       cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCTTATTCTTGAAcc                                                      >  1:370330/1‑141 (MQ=11)

                                                                                          tctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTTTTGAACCCaa                                                   >  2:203031/1‑141 (MQ=255)

                                                                                             gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                <  1:229051/139‑1 (MQ=255)

                                                                                              taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                               >  1:140341/2‑141 (MQ=19)

                                                                                              taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTACTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                               <  2:239250/140‑1 (MQ=255)

                                                                                              cATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCCTATTTTTGAACCCAAGGGt                                               >  2:42269/1‑141 (MQ=255)

                                                                                              cATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGt                                               <  2:301123/141‑1 (MQ=40)

                                                                                               aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTa                                              <  1:5026/141‑1 (MQ=18)

                                                                                                tCAAAGGCCCGATATAAATAGCGCCATTTTCCTTTTATTTTGATATAGGGATCATAAATCCGCCAATTGTAATAAGCTTTTTTATGCTTTTTATTCCAACTTTGATATAAAATTGGAGCAAATCATTTCACCCACCGGTAg                                             >  2:106308/1‑141 (MQ=2)

                                                                                                 cAATGGCACGATATAAATAGCTCCATTTCCCTATTATTTTGATCTACTTCTCATCAATACGCCATATATCATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAATTTGGGGCAGATTCTTGTACCCAActgaaaa                                            >  1:146238/1‑135 (MQ=14)

                                                                                                    tcgCACGACATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCg                                         <  2:186411/139‑1 (MQ=255)

                                                                                                      gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACCCCATTTGTAATAAGCTTTTTTATCCTTTTTCTTCCAAATTTGATATAAAATTGGTTCATATTCTTGAACCCAACGGCAGACCGtt                                       >  2:33416/1‑141 (MQ=11)

                                                                                                       caggctataAATCGCACCAATTTCCTTTTATTTTGATGTGCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTg                                      >  2:177771/6‑141 (MQ=14)

                                                                                                       cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCCCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTg                                      <  1:356559/141‑1 (MQ=18)

                                                                                                         cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGaa                                    >  1:188955/1‑141 (MQ=18)

                                                                                                         cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                    >  1:117103/1‑141 (MQ=255)

                                                                                                          gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAat                                   >  2:162889/1‑141 (MQ=255)

                                                                                                            tataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatga                                 >  2:137481/1‑141 (MQ=21)

                                                                                                            tataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatga                                 <  2:257498/141‑1 (MQ=18)

                                                                                                            tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                 <  1:251573/141‑1 (MQ=255)

                                                                                                             ataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgat                                >  2:88493/1‑141 (MQ=21)

                                                                                                             ataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgat                                >  1:109783/1‑141 (MQ=255)

                                                                                                              taAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTCGAatgatg                               >  2:338258/1‑141 (MQ=21)

                                                                                                              taAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgatg                               >  1:287128/1‑141 (MQ=255)

                                                                                                              taAATAGCTCCATTTTCCTTTTATTTTGAGGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTTAACCCAACGGTAGACCGTTGAatgatg                               >  2:368513/1‑141 (MQ=255)

                                                                                                                aaTAGCTTCATTTTCCTTTTATTTTGATAGACGGCTCATCAATACGCCATTTGTTATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                             <  1:199158/141‑1 (MQ=11)

                                                                                                                    gcgccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                         >  2:320305/4‑141 (MQ=255)

                                                                                                                      tCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCACCGGTAGCTCGTTGAATGATGAACGTTTa                       >  1:328698/1‑141 (MQ=11)

                                                                                                                        cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTaca                     <  2:133663/141‑1 (MQ=21)

                                                                                                                         aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCACCGGTAGATCGTTGAATGATGAACGTTTaccc                    >  1:36324/1‑139 (MQ=11)

                                                                                                                          ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAGCGGTAGATCGTTGAATGATGAACGTTTAcacc                   <  1:273985/141‑1 (MQ=11)

                                                                                                                          ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                   <  1:173635/141‑1 (MQ=255)

                                                                                                                          ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                   >  1:13671/1‑141 (MQ=255)

                                                                                                                          ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATCTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTCcacc                   >  1:178421/1‑141 (MQ=11)

                                                                                                                           tttCCTTTTATTTTGATATACGTCTCATTAATACGCCCTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                  <  1:92608/141‑1 (MQ=11)

                                                                                                                           ttcccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAACTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacacaa                  <  2:193268/138‑3 (MQ=11)

                                                                                                                            ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccac                 <  2:224916/141‑1 (MQ=25)

                                                                                                                            ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                 <  2:341953/141‑1 (MQ=255)

                                                                                                                              ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt               >  2:340574/1‑141 (MQ=255)

                                                                                                                              ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGGTTACACCATGt               >  1:27798/1‑141 (MQ=11)

                                                                                                                               tttttATTTTGATATACGTCTCATCTATACGCCATTTTTAATAAGCTTTTTTATGTTTTTTCTTCCAAATTTTATATACCATTAGAACATATTTTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt              <  1:166440/140‑1 (MQ=11)

                                                                                                                               cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt              >  1:226229/1‑141 (MQ=21)

                                                                                                                               cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt              >  2:252006/1‑141 (MQ=255)

                                                                                                                                  ttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTTAATGATGAACGTTTAcaccgagtcttt           >  2:63965/1‑133 (MQ=11)

                                                                                                                                   tATTTTGATATACGTCTAATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctct          >  2:75585/1‑141 (MQ=11)

                                                                                                                                    aTTTTGATATACGTCTCATCAAAACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGCCGCATATTCTTGAACCCCACGGTAGATCCTTGAATGATGAACGTTTACACCACGTTCTCtt         <  1:76743/141‑1 (MQ=11)

                                                                                                                                      tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa       <  1:97792/141‑1 (MQ=18)

                                                                                                                                        tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAata     >  1:16262/1‑141 (MQ=255)

                                                                                                                                         gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTGGAATGATGAACGTTTACACGACGTTCCCTTAatat    >  1:57856/1‑141 (MQ=255)

                                                                                                                                         gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat    >  1:217070/1‑141 (MQ=40)

                                                                                                                                         gATATACGGCTCATTAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat    <  1:153933/141‑1 (MQ=11)

                                                                                                                                          aTGTACGTCTCATGAATACGCCAGTTGGAGTAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGTATATTGTTGAAACCACCGGTAGACCGCTGAATGTTGAACGTTTACACCACGTTCCCTTAATAtt   <  2:156172/141‑1 (MQ=11)

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTT  >  CP000730/36175‑36454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`