BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,444	C→T	54.8%	R36R (AGG→AGA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,444	0	C	T	54.8%	30.8 / 307.0	252	R36R (AGG→AGA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (50/64); new base T (65/73); total (115/137)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 256 total aligned reads displayed.

TTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTA  >  CP000730/36305‑36583

 tATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                                                                                           >  1:158355/1‑141 (MQ=21)

 tATTTTGATATACGTCTAATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctct                                                                                                                                           >  2:75585/1‑141 (MQ=11)

  tttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                                                          <  2:89621/140‑1 (MQ=255)

  tttttGATAGACGGCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                                                          <  1:60274/140‑1 (MQ=11)

  aTTTTGCTGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTATTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                                                          <  1:90320/141‑1 (MQ=255)

  aTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                                                          <  2:139761/141‑1 (MQ=18)

  aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                                                          <  1:162889/141‑1 (MQ=255)

   ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                                                                                         <  1:337736/141‑1 (MQ=255)

    tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                                                                                        <  1:279223/141‑1 (MQ=21)

    tttGATATACGTCTCGTCAATACGCCGTTTGTAACAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTCGAACCCATCGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                                                                                        <  1:232458/141‑1 (MQ=11)

      tgttctccGTCTATTCAACACTCCATTTGTAATAAGCTTTTTTATGCCTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGATCCCAGCGGTATATCGTTGAATAATGATCGTTTACACCTCGTGCTCTTAata                                                                                                                                      <  2:27948/134‑1 (MQ=11)

       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                                                     >  2:135371/5‑141 (MQ=255)

       gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTGGAATGATGAACGTTTACACGACGTTCCCTTAatat                                                                                                                                     >  1:57856/1‑141 (MQ=255)

        atatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAtt                                                                                                                                    <  2:104823/138‑1 (MQ=255)

         tatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCCTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAttt                                                                                                                                   <  2:165028/139‑1 (MQ=11)

         tGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAttt                                                                                                                                   <  1:182769/141‑1 (MQ=21)

           tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCa                                                                                                                                 <  1:84120/141‑1 (MQ=255)

           tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCa                                                                                                                                 >  1:130915/1‑141 (MQ=255)

           tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTGTACACCACGTTCCCTTAATAtttta                                                                                                                                 >  1:70146/1‑139 (MQ=255)

           tACGTCTCATCAATACGCCATTTGTAATAAGCTCTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATACTTCa                                                                                                                                 <  1:260091/141‑1 (MQ=11)

            aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                                                                                                >  1:334210/1‑141 (MQ=255)

             cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAGTATTTCAGa                                                                                                                               >  1:244615/1‑141 (MQ=11)

             cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                                                                                               <  2:290343/141‑1 (MQ=255)

             cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                                                                                               >  2:315768/1‑141 (MQ=255)

              gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTTAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGat                                                                                                                              <  2:326927/141‑1 (MQ=21)

              gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                                                                                              <  2:309085/141‑1 (MQ=255)

              gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGat                                                                                                                              <  2:374229/141‑1 (MQ=25)

               tctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGata                                                                                                                             <  1:361634/141‑1 (MQ=18)

                ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTACTATTTCacacaa                                                                                                                            >  2:166801/1‑136 (MQ=255)

                ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatat                                                                                                                            >  2:321464/1‑141 (MQ=40)

                  catcaATACGCCGTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCGGTTCCCTTAATATTTCAGatatat                                                                                                                          >  1:270659/1‑141 (MQ=11)

                  catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                                                                                          <  1:161340/141‑1 (MQ=255)

                  catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                                                                                          >  1:65519/1‑141 (MQ=255)

                  catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatac                                                                                                                          >  2:148649/1‑140 (MQ=255)

                   atcaATACGCCGTTAGTAATAAGCTTTGTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAGCGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                                                                                         >  2:185342/1‑141 (MQ=11)

                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                                                                                         <  2:117103/141‑1 (MQ=255)

                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                                                                                         <  1:333039/141‑1 (MQ=255)

                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                                                                                         <  1:48749/141‑1 (MQ=255)

                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                                                                                         >  1:217360/1‑141 (MQ=21)

                   atcaAAACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                                                                                         <  1:105788/141‑1 (MQ=11)

                    tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                                                                                        >  1:277062/1‑141 (MQ=255)

                    tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                                                                                        >  1:227437/1‑141 (MQ=255)

                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGGTCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATGAc                                                                                                                       >  1:125069/1‑141 (MQ=11)

                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                                                                                       >  2:17275/1‑141 (MQ=255)

                      actatgCCATTTGTAATAAGCTTTTTTATGCTTATTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACg                                                                                                                      <  2:199763/136‑1 (MQ=255)

                      aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCGACGGTAGATCGCTGTTCGATGATCTTTTACACCACGTTCTCTTAATATTTCAGATGTAGCACg                                                                                                                      >  1:29411/1‑141 (MQ=11)

                       aTACGGCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATATCAGATATATCACGa                                                                                                                     <  1:284503/141‑1 (MQ=11)

                       aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                                                     <  1:299804/141‑1 (MQ=255)

                       aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                                                     <  2:334210/141‑1 (MQ=255)

                        tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGTCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAGTTCAGATATATCACGAt                                                                                                                    >  2:255930/1‑141 (MQ=11)

                        tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                                                                                    >  1:251723/1‑141 (MQ=255)

                        tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                                                                                    >  1:17721/1‑141 (MQ=255)

                        tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGAt                                                                                                                    <  1:374273/141‑1 (MQ=18)

                         tcGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                                                                                   <  2:293007/140‑1 (MQ=255)

                          cgcaacttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGTCCTATTCTTGAACCCAACGGTAGATCGGTGAATGCTGAACGTTTACACCACGTTCTCTTAATATTTCAGATGTATCACGATaa                                                                                                                  >  2:177864/7‑141 (MQ=11)

                          cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                                                  >  1:359327/1‑140 (MQ=255)

                          cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                                                  <  1:138265/141‑1 (MQ=21)

                           gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                                                                                 <  1:135355/141‑3 (MQ=255)

                           gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                                                                                                 >  2:44623/1‑141 (MQ=40)

                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                                                >  1:222195/1‑141 (MQ=255)

                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                                                                                >  1:115358/1‑141 (MQ=21)

                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGACATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCAAGTTCCCTTAATATTTTAAATATATCACGATAAcc                                                                                                                >  1:35805/1‑140 (MQ=11)

                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                                                                                >  1:99578/1‑141 (MQ=40)

                              aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                                              >  1:335529/1‑141 (MQ=25)

                              aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                                              <  1:43367/141‑1 (MQ=21)

                              aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                                              >  2:358415/1‑141 (MQ=21)

                              aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                                              >  1:151235/1‑141 (MQ=21)

                              aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAAGATAACTCa                                                                                                              >  2:230360/1‑141 (MQ=21)

                              aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGCTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                                              <  1:262770/141‑1 (MQ=21)

                               tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAGATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                                                             <  2:304769/141‑1 (MQ=21)

                               tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                                                             <  1:382460/141‑1 (MQ=255)

                               tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                                                             <  1:169114/141‑1 (MQ=255)

                               tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTCATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCATATATATCACGATAGCTCaa                                                                                                             <  1:249191/141‑1 (MQ=11)

                                ttGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                            >  1:203132/1‑141 (MQ=11)

                                ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGTATATTCTTGAACCCAACGGTAGACCGTTGAATCATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATATCTCAAt                                                                                                            <  2:240596/141‑1 (MQ=255)

                                ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                            >  2:50230/1‑141 (MQ=255)

                                ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGGTCTCTTAATATTTCAGATATATCACGGTAGCTCAAt                                                                                                            >  1:141350/1‑141 (MQ=11)

                                ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                            >  2:324826/1‑141 (MQ=21)

                                ttGTAATAAGCTTTTTTATGCTTTTTCTGCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                            <  2:23501/141‑1 (MQ=11)

                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTTAATGATGAACCTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                           <  1:74030/141‑1 (MQ=11)

                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                           >  1:307828/1‑141 (MQ=21)

                                  gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATGGCTCAATgc                                                                                                          >  1:21518/1‑140 (MQ=11)

                                  gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTCCAGATATATCACGATAGCTCAATgc                                                                                                          >  2:46950/1‑140 (MQ=11)

                                    aataaGCTTTTTTATGTTTTTTCGTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgcat                                                                                                        <  2:345458/141‑4 (MQ=9)

                                    aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgcat                                                                                                        >  1:172611/1‑138 (MQ=11)

                                    aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgcat                                                                                                        >  1:110084/1‑138 (MQ=11)

                                           ttttttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAg                                                                                                 <  2:15003/141‑1 (MQ=35)

                                                    ttttttCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGcc                                                                                        <  1:93022/140‑1 (MQ=25)

                                                          cttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGc                                                                                  >  1:144801/1‑141 (MQ=35)

                                                           ttcCAAATTTGATACCAAATTGGGGTATATTCTTGAACCCAACGGCAGACCGTTGGATGATGAACGCTTACACCACGTTCCCTTAATATTTCAGATATATCACGTTAACTCCATGTATATTTTAGATAGTAGCCAACGTCt                                                                                 >  1:192364/1‑141 (MQ=11)

                                                            tcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCGATCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCtc                                                                                >  2:211615/1‑140 (MQ=25)

                                                                  tttGATACAAAATTGGGGCATATTCTTGAACCCAACGTTAGACCGTTGAATGATGAACGTTTACACCAAGTTCCCTTAATATTTCATATATATCACGATAACCCACTGTATATCTAAGATAGTTGCCAACGGCTACAATGa                                                                          >  2:129719/1‑141 (MQ=11)

                                                                                  ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaaa                                                          >  1:376064/1‑141 (MQ=32)

                                                                                           ctGAACCCACCGGTAGCCCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTtata                                                 <  2:8009/140‑1 (MQ=17)

                                                                                              aaCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAAAATCCTTGTTAAATTGTTTATATCt                                              >  2:144812/1‑141 (MQ=17)

                                                                                               aCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTg                                             <  2:53992/141‑1 (MQ=32)

                                                                                                          gACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTCGCCAACGTCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCAt                                  >  1:165866/1‑141 (MQ=17)

                                                                                                                  aaTGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACACAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACagaaga                          <  2:156919/141‑1 (MQ=17)

                                                                                                                            gTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTg                <  1:316012/141‑1 (MQ=32)

                                                                                                                                          cccTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACta  >  2:26746/1‑141 (MQ=32)

TTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTA  >  CP000730/36305‑36583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`