Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,817 A→T 35.8% intergenic (+48/+60) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8170AT35.8% 123.2 / 83.4 87intergenic (+48/+60)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (25/30);  new base T (8/23);  total (34/53)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.41e-01

GCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATA  >  CP000730/35677‑35956
                                                                                                                                            |                                                                                                                                           
gCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGaa                                                                                                                                             >  1:37991/1‑141 (MQ=255)
gCAAATTAATCTGAATTTAAAGATGTAGAAGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAAAAAAAAAAAAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGaa                                                                                                                                             <  2:355775/141‑1 (MQ=255)
  aaaTTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAAAAAAAATAGAAgg                                                                                                                                           >  1:49337/1‑141 (MQ=255)
   aaTTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGt                                                                                                                                          <  1:350578/141‑1 (MQ=255)
     ttattCTGAGTTGAAGGATGTAGATGCACTCACGGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTc                                                                                                                                        <  1:86506/141‑1 (MQ=255)
       attCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAACATTGTGATCTAATAAAAATAGAAGGTTCTg                                                                                                                                      >  1:36211/1‑141 (MQ=255)
           tGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGc                                                                                                                                  <  2:348991/141‑1 (MQ=255)
                ttAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGt                                                                                                                             <  1:139324/141‑1 (MQ=255)
                    aGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAg                                                                                                                                            <  2:354370/122‑1 (MQ=255)
                    aGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAg                                                                                                                                            >  1:354370/1‑122 (MQ=255)
                     gATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaaa                                                                                                                        >  1:26462/1‑141 (MQ=255)
                       tGTATATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaaaaa                                                                                                                      <  1:312957/141‑1 (MQ=255)
                             tgatCTCATTGACACAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGc                                                                                                                >  2:329035/1‑141 (MQ=255)
                                          aaaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAAttt                                                                                                   <  2:118183/141‑1 (MQ=255)
                                          aaaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTGGGCGAAAAATGATTACTTGAACAAGTGCTAAAATAAAATTGTGATCTATTAACAATTGTAGGTTTTGATAAAAAAAAAAAAAATATAGCTAACCACTAAttt                                                                                                   <  2:14841/141‑1 (MQ=255)
                                          aaaGTATTTAATGTAGAAGCCATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGAACTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAAttt                                                                                                   <  2:178454/141‑1 (MQ=255)
                                           aaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTa                                                                                                  >  1:177984/1‑141 (MQ=255)
                                                tttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATg                                                                                             >  2:134912/1‑141 (MQ=255)
                                                tttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATg                                                                                             >  2:289885/1‑141 (MQ=255)
                                                tttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATg                                                                                             <  1:323201/141‑1 (MQ=255)
                                                 ttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGt                                                                                            <  2:44254/141‑1 (MQ=255)
                                                    aTGTACAAGCCACTCAGTATAAAGTTTTTGGCCAAAAATGAGTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGTTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAg                                                                                         <  1:48866/141‑1 (MQ=37)
                                                             cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCtt                                                                                <  1:176168/141‑1 (MQ=255)
                                                             cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCtt                                                                                <  1:207222/141‑1 (MQ=255)
                                                                  cAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACtt                                                                           >  1:61403/1‑141 (MQ=255)
                                                                    ggataAAGTTTTTGGCGGAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGc                                                                         <  2:23588/139‑1 (MQ=255)
                                                                    ggataAAGTTTATGGAGAAAAATGATTACATGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTATGAAGAAAAAAAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGc                                                                         <  2:182102/139‑1 (MQ=25)
                                                                    ataggaaGTTTTTGGCGGAAAATGATGAATTGCAGTAGGGCCAAATTAAAATTGAAAAATAAAAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGc                                                                         <  2:208359/136‑1 (MQ=17)
                                                                     tataAAGTTTTTGGCGAAAAATGATTACTTGAACACGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAACAATATCGCTAACCACTAATTTTTCATGTCAGTGTTCGCTTAACTTGCt                                                                        <  2:280519/141‑1 (MQ=255)
                                                                     tataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCt                                                                        <  1:99099/141‑1 (MQ=255)
                                                                         aaGTTTTTGGCGAAACATGCTTACTTGAACAAGAGCTAAAATAAAATTGTGATCTACTAAAACCAGAAGGCTCTGTTGCACAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTCTTCGCTTAACTTGCTaccc                                                                    >  2:349042/1‑138 (MQ=21)
                                                                         aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAACAATAGAAGGTTCTGTTGCAAAGTAAAAAAATGTAGCTAACCACTAATTTATCATGTCAGTGTTCGCTGAACTTGCTAGCa                                                                    >  2:168274/1‑141 (MQ=255)
                                                                              tttGGCGAAAAATGATAACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                               >  1:195544/1‑141 (MQ=255)
                                                                              caggggggtagaTGATTACTATAACAAGAGCTAAAATAAAATTGTGATCTAATAACAATAGAAGGTTCTGTTGAAAAGTAAAAAAATATAGCTAACCACTAATGTAGCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                               <  2:94336/130‑1 (MQ=18)
                                                                               ttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGc                                                              <  1:343172/141‑1 (MQ=255)
                                                                               taggaaaaaaaTGACTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTAAAAAAAAAAAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGc                                                              <  2:99470/135‑1 (MQ=21)
                                                                                    gAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGCCAGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTGGCTAGCATGATGCTAAtt                                                         >  1:72633/1‑141 (MQ=255)
                                                                                    gAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGACTTCGCGTACCTTGCTAGCATGATGCTAAtt                                                         >  2:239565/1‑141 (MQ=255)
                                                                                      aaaaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTc                                                       >  2:50591/1‑141 (MQ=255)
                                                                                                   gaacaaagagtaaaataaaaTTGTAATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaa                                          <  2:303541/131‑1 (MQ=255)
                                                                                                        agagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                     >  1:320738/1‑141 (MQ=255)
                                                                                                        agagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGCCACTCGTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                     >  1:358312/1‑141 (MQ=255)
                                                                                                         gagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAGCTTGCTAGCATGATGCTAATTTCGTGGCCTGGCGAAAATCCg                                    >  1:2429/1‑141 (MQ=255)
                                                                                                         gagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCg                                    >  2:259048/1‑141 (MQ=255)
                                                                                                          agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                   <  2:26462/141‑1 (MQ=255)
                                                                                                                 acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                          <  1:3457/127‑1 (MQ=11)
                                                                                                                 acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                          >  1:28203/15‑141 (MQ=11)
                                                                                                                  taaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCGTGGCGAAAATCCGTACATCTGa                           >  1:256736/1‑141 (MQ=255)
                                                                                                                  caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                         <  1:54206/128‑1 (MQ=11)
                                                                                                                  caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATAACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                         >  1:230165/14‑141 (MQ=11)
                                                                                                                    aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                       <  1:31791/130‑1 (MQ=11)
                                                                                                                     aagagtattttaataaAAATGATGGTGCTTGTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                      <  1:362686/131‑1 (MQ=11)
                                                                                                                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATTTATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  1:50098/132‑1 (MQ=11)
                                                                                                                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  1:136768/132‑1 (MQ=11)
                                                                                                                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  1:236434/132‑1 (MQ=11)
                                                                                                                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  1:60447/132‑1 (MQ=11)
                                                                                                                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  2:53954/132‑1 (MQ=11)
                                                                                                                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGACCTGAAGAGAc                     >  1:288450/10‑141 (MQ=11)
                                                                                                                       gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                    <  1:361002/133‑1 (MQ=11)
                                                                                                                         ttgATTTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                    <  2:37991/140‑1 (MQ=255)
                                                                                                                         gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                  <  1:243031/135‑1 (MQ=11)
                                                                                                                          tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTCGATCTGAAGAGACCTGc                 <  2:192795/136‑1 (MQ=11)
                                                                                                                          tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                 <  2:196476/136‑1 (MQ=11)
                                                                                                                          tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                 <  1:366229/136‑1 (MQ=11)
                                                                                                                           taTCTAATAAAAATAGAAGGGTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATGTATCATGTCAGTGTTCGCTTAACTTGCTAGCATTATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                  <  1:364514/140‑1 (MQ=255)
                                                                                                                           attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                >  2:258630/5‑141 (MQ=11)
                                                                                                                           attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                >  2:312142/5‑141 (MQ=11)
                                                                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               <  1:81635/138‑1 (MQ=11)
                                                                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               <  1:245200/138‑1 (MQ=11)
                                                                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCGAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               <  2:291873/138‑1 (MQ=11)
                                                                                                                            aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                 >  1:216593/1‑141 (MQ=255)
                                                                                                                             tttaataaAAATTATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTCATATATCATGTCAGTGTTCGTTTAACCTGCTAGTTTGATGCTAATTTCGTGGTATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              <  1:174426/139‑1 (MQ=11)
                                                                                                                             tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              <  2:313926/139‑1 (MQ=11)
                                                                                                                             tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              >  2:312786/3‑141 (MQ=11)
                                                                                                                             tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTCCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              <  1:162065/139‑1 (MQ=11)
                                                                                                                             tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCAATAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGAGCTGAAGAGACCTGCGGt              >  1:75182/3‑141 (MQ=11)
                                                                                                                             tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATACAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              <  2:208841/139‑1 (MQ=11)
                                                                                                                             tCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTAAagtggcgcgcg                >  2:57802/1‑132 (MQ=255)
                                                                                                                              ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt             <  2:68014/140‑1 (MQ=11)
                                                                                                                               taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc            <  2:244793/141‑1 (MQ=11)
                                                                                                                                aataaAAATGGAAGTTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTGATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt             <  2:182580/141‑1 (MQ=255)
                                                                                                                                 ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGTAGAGACCTGCGGTTCtt          >  2:257491/1‑141 (MQ=11)
                                                                                                                                  taaAAATAGAAGGTTCTGTTGCAAAGTAAACAAATATAGCTAACCACTAATTTATCACGTCCGTGTTCGCTTAACTTGCTAGCAGGAAGCTAATTTCGTGGCAGGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGGTCt           >  2:39149/1‑141 (MQ=11)
                                                                                                                                  taaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATTTAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt           >  2:47803/1‑141 (MQ=38)
                                                                                                                                  taaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt           >  1:277386/1‑141 (MQ=38)
                                                                                                                                     aaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGCGTTCGCTCAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtttt        <  1:182349/141‑1 (MQ=25)
                                                                                                                                       aTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAGCTGCGGTTCTTTTta      <  1:284926/141‑1 (MQ=18)
                                                                                                                                        ccgAAGTTTCTTTTGCAAAGTAAAAAATATAGCTACTCACTAATATATCATGTCAGTGTTCGTTTAGCTTGCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata    <  2:284489/139‑1 (MQ=11)
                                                                                                                                         aGAAGGTTCTGTGGCAAAGTAAAAAAATATAGCTAACAACTGATTTATCATATCAGTGTTCGATTAACTTACTAGCATGATGCTAATTCCGTGGCATGGCGAAAGTCCGTAGATCTGAAGAGACCTGCGGTGCttttgaga    >  2:95131/1‑137 (MQ=11)
                                                                                                                                           aaGTTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCCAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatata  <  2:256738/141‑1 (MQ=11)
                                                                                                                                           aaGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGTCCTGCGGTTCTTTTtatata  <  1:379500/141‑1 (MQ=11)
                                                                                                                                            |                                                                                                                                           
GCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATA  >  CP000730/35677‑35956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: