Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,744,454 C→T 100% G73D (GGC→GAC)  apt ← adenine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,744,4540CT95.9% 67.0 / ‑5.9 25G73D (GGC→GAC) aptadenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (14/9);  minor base G (1/1);  total (15/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAC  >  CP000730/1744320‑1744583
                                                                                                                                      |                                                                                                                                 
cTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTGTCTAACAGGTGCAAAGTCAATcc                                                                                                                             >  2:65545/1‑141 (MQ=255)
  gTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATcccc                                                                                                                           <  2:304332/141‑1 (MQ=255)
   tacttAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACAATTGTACCATATTCTAGATCATACTCATAACGTATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACACGTGCCACGTCAATccccc                                                                                                                          >  2:233928/4‑140 (MQ=255)
     aTTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCAtt                                                                                                                        >  2:60481/1‑141 (MQ=255)
       tAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGCGGTAATTTCCCTTCTTTTCTAACAGGTGCAAATGCAATCCCCATTGa                                                                                                                      >  2:153848/1‑141 (MQ=255)
               gTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTa                                                                                                              <  2:166676/141‑1 (MQ=255)
                  gACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTTATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATACGATACAg                                                                                                           >  1:296225/1‑141 (MQ=255)
                  gACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGCCATACTCATAACGAATGACTTCACGAGGTTATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCACCATTGAATAAGCTACAg                                                                                                           >  1:296242/1‑141 (MQ=255)
                    ccTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGa                                                                                                         >  1:284833/1‑141 (MQ=255)
                    ccTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGa                                                                                                         <  1:45238/141‑1 (MQ=255)
                                      ttGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCa                                                                                       <  1:311747/141‑1 (MQ=255)
                                         tgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCAcgc                                                                                    >  1:71209/1‑141 (MQ=255)
                                                ttAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAg                                                                             >  1:344821/1‑141 (MQ=255)
                                                    aaCATTTGTACCATCTTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGAAAGCCAATGATAAAGCCACGCGCTTCAGGTcc                                                                         <  2:368300/141‑1 (MQ=255)
                                                     aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCt                                                                        <  2:344454/141‑1 (MQ=255)
                                                            tACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGAAAGCCAATGTTAAAGCCACGCGATTCAGGTCCTACAACGa                                                                 >  1:71556/1‑141 (MQ=255)
                                                                   ttCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAAc                                                          <  2:248513/141‑1 (MQ=255)
                                                                         gTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATctct                                                    <  2:152669/141‑1 (MQ=255)
                                                                             tACTCATAACGAATGACTTCACGAGGTACTTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGACTAAGCTACAGGCAGGACAATGATAAACCCACGCGCTTCGGGTCCTACAACGATATCAACATCTGTGTCt                                                >  2:21597/1‑141 (MQ=255)
                                                                               cTCATAACGAATGACTTCACGAGGTAATTGCCCTTCTTTACTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATCACAACAACTCTGTCttt                                              >  2:110981/1‑141 (MQ=255)
                                                                                 cATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg                                            >  2:84618/1‑141 (MQ=255)
                                                                                                   gagatAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTAt                          >  1:300484/5‑141 (MQ=255)
                                                                                                                   ttttCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCata          >  1:235974/1‑141 (MQ=255)
                                                                                                                    tttCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTCCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCatat         <  1:269906/141‑1 (MQ=255)
                                                                                                                           cAGGTGCAAAGGCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAc  <  1:153848/141‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                 
CTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAC  >  CP000730/1744320‑1744583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: