Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,749,286 C→T 14.5% D55N (GAC→AAC)  secDF ← RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,749,2860CT14.5% 57.0 / 4.4 29D55N (GAC→AAC) secDFRND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter
Reads supporting (aligned to +/- strand):  ref base C (15/9);  new base T (2/2);  total (18/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

CACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGA  >  CP000730/1749157‑1749420
                                                                                                                                 |                                                                                                                                      
cacCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGCTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCcttattta                                                                                                                             >  1:54861/1‑141 (MQ=255)
      cAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAACACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGAt                                                                                                                       >  1:23420/1‑141 (MQ=255)
        aGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCAAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTACTTAAAGGATCg                                                                                                                     >  1:42175/1‑141 (MQ=255)
                tctAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATa                                                                                                             <  2:23420/141‑1 (MQ=255)
                         aTTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTc                                                                                                    >  2:328214/1‑141 (MQ=255)
                                 tttcGACTTGAATTTTATGCTCCGAAGCACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCAc                                                                                            <  2:351406/140‑1 (MQ=255)
                                     aaCTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTTGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTg                                                                                        >  2:243252/1‑141 (MQ=255)
                                     aaCTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTTGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTg                                                                                        >  2:243137/1‑141 (MQ=255)
                                      aCTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGCTTGTAAGGCTTTTTTAGCTATTTTGTCGCCTTTGTTTAAGGGATCGACTTGATGAAACGCCTCAAATCCACCTTGc                                                                                       >  1:12151/1‑141 (MQ=255)
                                      aCTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTACGGCTTTTTTATCTATTTTGTGACCTTTACGCAGATGATGGACTTGAGAAATCACGTCAGATCCACCTTGc                                                                                       >  2:33302/1‑141 (MQ=255)
                                      aCTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATATATTTTATCGCCTTTATTTAAAGGATTGATTTTATAAAGCATCTAAAATCCACCTTGc                                                                                       >  2:346841/1‑141 (MQ=255)
                                       cTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGGTAAAGCACCTCAAATCCACCTTGCa                                                                                      >  1:337022/1‑141 (MQ=255)
                                        ttGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCaa                                                                                     >  1:35889/1‑141 (MQ=255)
                                         tGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCaaa                                                                                    <  1:25282/141‑1 (MQ=255)
                                                              ccTAAAAAGTTAACACGATTTTCTTATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCGCAAATCCACCTTGCAAATCAAGTCCTAAATTGCCATTc                                                               >  1:243149/1‑141 (MQ=255)
                                                                 aaaaCGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCttt                                                            <  1:15823/141‑1 (MQ=255)
                                                                  aaaCGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTta                                                           <  1:361582/141‑1 (MQ=255)
                                                                  aaaCGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTta                                                           <  1:90888/141‑1 (MQ=255)
                                                                           cacGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACttt                                                  >  2:183607/1‑141 (MQ=255)
                                                                                       aTGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTTGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAg                                      <  1:243252/141‑1 (MQ=255)
                                                                                       aTGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTTGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAg                                      <  1:243137/141‑1 (MQ=255)
                                                                                          tttGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCa                                   >  2:123236/1‑141 (MQ=255)
                                                                                                cTGTTGATTGTAAGGCTTTTTTTTCTATTTTGTCGCCTTGATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCgg                             >  2:34972/1‑141 (MQ=255)
                                                                                                cTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATCCCgg                             <  1:259968/141‑1 (MQ=255)
                                                                                                       ttGTAAGGCTTTTTTTTTTTTTTTTTCGCCTTTATTTAAAGGATCGACTTGATAAAGCGCCTCAAAGCCACCTTGCAAGTCAAGTCCTAAATTGACATTCTTTATAACACTTTTATATGTTGCATCCTTTCCGGCAaacaa                      >  2:165218/1‑141 (MQ=255)
                                                                                                        tGTACGTCTTTTTTATCGATTTTGTCGCCTTTATTCAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAACTTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAaacaac                     <  1:128543/141‑1 (MQ=255)
                                                                                                                ttttttATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGAc             <  2:359425/141‑1 (MQ=255)
                                                                                                                    ttATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTaaa         >  1:115114/1‑141 (MQ=255)
                                                                                                                           ttttGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACATTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAATAGCAAGa  >  2:261294/1‑141 (MQ=255)
                                                                                                                                 |                                                                                                                                      
CACCAGCAAGTTGTACTCTAATTCTATTAGGTTCTTCAACTTGAATTTTAGGTTCCGAAACACCTAAAACGTTAACACGATTTTCTAATGTTTGCGCTGTTGATTGTAAGGCTTTTTTATCTATTTTGTCGCCTTTATTTAAAGGATCGACTTGATAAAGCACCTCAAATCCACCTTGCAAATCAAGTCCTAAATTGACATTCTTTATAACACTTTTATAAGTTGCAGCCATTCCGGCAAACAACAATACGACTAAAAGCAAGA  >  CP000730/1749157‑1749420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: