Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,876 C→T 52.7% intergenic (+107/+1) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8760CT52.7% 33.1 / 153.4 126intergenic (+107/+1)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (23/36);  new base T (29/37);  total (53/73)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 129 total aligned reads displayed.

CTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACC  >  CP000730/35738‑36016
                                                                                                                                          |                                                                                                                                             
cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCtt                                                                                                                                             <  1:176168/141‑1 (MQ=255)
cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCtt                                                                                                                                             <  1:207222/141‑1 (MQ=255)
     cAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACtt                                                                                                                                        >  1:61403/1‑141 (MQ=255)
       ggataAAGTTTTTGGCGGAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGc                                                                                                                                      <  2:23588/139‑1 (MQ=255)
       ggataAAGTTTATGGAGAAAAATGATTACATGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTATGAAGAAAAAAAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGc                                                                                                                                      <  2:182102/139‑1 (MQ=25)
       ataggaaGTTTTTGGCGGAAAATGATGAATTGCAGTAGGGCCAAATTAAAATTGAAAAATAAAAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGc                                                                                                                                      <  2:208359/136‑1 (MQ=17)
        tataAAGTTTTTGGCGAAAAATGATTACTTGAACACGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAACAATATCGCTAACCACTAATTTTTCATGTCAGTGTTCGCTTAACTTGCt                                                                                                                                     <  2:280519/141‑1 (MQ=255)
        tataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCt                                                                                                                                     <  1:99099/141‑1 (MQ=255)
            aaGTTTTTGGCGAAACATGCTTACTTGAACAAGAGCTAAAATAAAATTGTGATCTACTAAAACCAGAAGGCTCTGTTGCACAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTCTTCGCTTAACTTGCTaccc                                                                                                                                 >  2:349042/1‑138 (MQ=21)
                 tttGGCGAAAAATGATAACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                                                                                            >  1:195544/1‑141 (MQ=255)
                 caggggggtagaTGATTACTATAACAAGAGCTAAAATAAAATTGTGATCTAATAACAATAGAAGGTTCTGTTGAAAAGTAAAAAAATATAGCTAACCACTAATGTAGCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                                                                                            <  2:94336/130‑1 (MQ=18)
                       gAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGCCAGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTGGCTAGCATGATGCTAAtt                                                                                                                      >  1:72633/1‑141 (MQ=255)
                       gAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGACTTCGCGTACCTTGCTAGCATGATGCTAAtt                                                                                                                      >  2:239565/1‑141 (MQ=255)
                         aaaaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTc                                                                                                                    >  2:50591/1‑141 (MQ=255)
                                      gaacaaagagtaaaataaaaTTGTAATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaa                                                                                                       <  2:303541/131‑1 (MQ=255)
                                           agagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                                                                                  >  1:320738/1‑141 (MQ=255)
                                           agagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGCCACTCGTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                                                                                  >  1:358312/1‑141 (MQ=255)
                                            gagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAGCTTGCTAGCATGATGCTAATTTCGTGGCCTGGCGAAAATCCg                                                                                                 >  1:2429/1‑141 (MQ=255)
                                             agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                                                                                <  2:26462/141‑1 (MQ=255)
                                                    acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                                                       >  1:28203/15‑141 (MQ=11)
                                                    acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                                                       <  1:3457/127‑1 (MQ=11)
                                                     taaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCGTGGCGAAAATCCGTACATCTGa                                                                                        >  1:256736/1‑141 (MQ=255)
                                                     caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATAACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                                                      >  1:230165/14‑141 (MQ=11)
                                                       aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                                                                                    <  1:31791/130‑1 (MQ=11)
                                                         agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATTTATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:50098/132‑1 (MQ=11)
                                                         agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:60447/132‑1 (MQ=11)
                                                         agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:236434/132‑1 (MQ=11)
                                                         agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:136768/132‑1 (MQ=11)
                                                          gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                <  1:361002/133‑1 (MQ=11)
                                                             tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTCGATCTGAAGAGACCTGc                                                                             <  2:192795/136‑1 (MQ=11)
                                                             tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                             <  1:366229/136‑1 (MQ=11)
                                                              taTCTAATAAAAATAGAAGGGTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATGTATCATGTCAGTGTTCGCTTAACTTGCTAGCATTATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                              <  1:364514/140‑1 (MQ=255)
                                                              attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                            >  2:312142/5‑141 (MQ=11)
                                                              attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                            >  2:258630/5‑141 (MQ=11)
                                                               ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                           <  1:81635/138‑1 (MQ=11)
                                                               ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                           <  1:245200/138‑1 (MQ=11)
                                                               aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                             >  1:216593/1‑141 (MQ=255)
                                                                tttaataaAAATTATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTCATATATCATGTCAGTGTTCGTTTAACCTGCTAGTTTGATGCTAATTTCGTGGTATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                          <  1:174426/139‑1 (MQ=11)
                                                                tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                          <  2:313926/139‑1 (MQ=11)
                                                                tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                          >  2:312786/3‑141 (MQ=11)
                                                                tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTCCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                          <  1:162065/139‑1 (MQ=11)
                                                                tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCAATAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGAGCTGAAGAGACCTGCGGt                                                                          >  1:75182/3‑141 (MQ=11)
                                                                tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATACAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                          <  2:208841/139‑1 (MQ=11)
                                                                tCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTAAagtggcgcgcg                                                                             >  2:57802/1‑132 (MQ=255)
                                                                 ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                         <  2:68014/140‑1 (MQ=11)
                                                                    ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGTAGAGACCTGCGGTTCtt                                                                      >  2:257491/1‑141 (MQ=11)
                                                                     taaAAATAGAAGGTTCTGTTGCAAAGTAAACAAATATAGCTAACCACTAATTTATCACGTCCGTGTTCGCTTAACTTGCTAGCAGGAAGCTAATTTCGTGGCAGGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGGTCt                                                                       >  2:39149/1‑141 (MQ=11)
                                                                     taaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATTTAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                                                       >  2:47803/1‑141 (MQ=38)
                                                                     taaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                                                       >  1:277386/1‑141 (MQ=38)
                                                                          aTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAGCTGCGGTTCTTTTta                                                                  <  1:284926/141‑1 (MQ=18)
                                                                           ccgAAGTTTCTTTTGCAAAGTAAAAAATATAGCTACTCACTAATATATCATGTCAGTGTTCGTTTAGCTTGCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata                                                                <  2:284489/139‑1 (MQ=11)
                                                                            aGAAGGTTCTGTGGCAAAGTAAAAAAATATAGCTAACAACTGATTTATCATATCAGTGTTCGATTAACTTACTAGCATGATGCTAATTCCGTGGCATGGCGAAAGTCCGTAGATCTGAAGAGACCTGCGGTGCttttgaga                                                                >  2:95131/1‑137 (MQ=11)
                                                                              aaGTTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCCAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatata                                                              <  2:256738/141‑1 (MQ=11)
                                                                                                            ccaatcACTAATATTGCATGGCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAg                                <  1:10667/136‑1 (MQ=1)
                                                                                                                    tactttCTCATGTAAGTATTCGCTTAACTTGCGAGCATGATGCTAATTTCGTGGGATTGCGAAAAACCGTAGAACTAAAAGATCCTTCAATTCTTTGTATATCCTGCGTAAATACACTCAATACCTTTTTAATTATTCttt                        <  2:174431/138‑1 (MQ=0)
                                                                                                                      atatATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGCGACCTACGGTTCTATTGAGAGAACGCGTAAATAAATCCAATCCCTTTTAACCTATCCTTTGc                      >  2:79680/4‑141 (MQ=1)
                                                                                                                      atatATCATGTCAGCGTTCATTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGCCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGc                      >  1:210372/4‑141 (MQ=1)
                                                                                                                                 cattgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc           <  1:133948/138‑1 (MQ=11)
                                                                                                                                 cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc           >  2:381651/1‑141 (MQ=255)
                                                                                                                                 cAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAATAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc           <  2:285166/141‑1 (MQ=11)
                                                                                                                                 cAGCGTTCGTTTAACTTGCGAGCATGATGCTCATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc           <  1:76648/141‑1 (MQ=11)
                                                                                                                                  cgctgtCGCCTGACTTGCTTGTATCATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCGTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          <  2:154066/136‑1 (MQ=11)
                                                                                                                                  aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGTGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt          >  2:236646/1‑141 (MQ=11)
                                                                                                                                  aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt          >  1:103933/1‑141 (MQ=11)
                                                                                                                                  aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          >  1:161176/1‑141 (MQ=9)
                                                                                                                                  aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGCAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          <  2:54776/141‑1 (MQ=9)
                                                                                                                                   gttttCGTTTAACGTGCTAGCATGATGCTAATTCCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt         <  2:13114/138‑1 (MQ=11)
                                                                                                                                   gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt         >  1:173544/1‑141 (MQ=12)
                                                                                                                                   gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt         >  1:335882/1‑141 (MQ=17)
                                                                                                                                   gggtTCGTTTACTTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTACATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt         <  1:241656/139‑1 (MQ=11)
                                                                                                                                   gggtTCGTTTAACTTGCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt         <  2:35393/139‑1 (MQ=12)
                                                                                                                                    tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt        >  2:193922/1‑141 (MQ=255)
                                                                                                                                    tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTAAATACCTTTTAAAGTATTATTAGCCGTATTGATACttt        >  1:294121/1‑141 (MQ=11)
                                                                                                                                    tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACAATCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt        >  2:194827/1‑141 (MQ=11)
                                                                                                                                    tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt        >  1:283160/1‑141 (MQ=12)
                                                                                                                                    tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt        >  2:11277/1‑141 (MQ=17)
                                                                                                                                     gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg       >  1:238530/1‑141 (MQ=255)
                                                                                                                                      ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa      <  1:371890/141‑1 (MQ=255)
                                                                                                                                      ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa      >  1:46744/1‑141 (MQ=11)
                                                                                                                                      ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa      <  2:299028/141‑1 (MQ=255)
                                                                                                                                      ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  1:315140/141‑1 (MQ=12)
                                                                                                                                      ttCGTTTAACGTGCTAGCCGGATGCTAAGTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGATCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  1:176677/141‑1 (MQ=11)
                                                                                                                                      ttCGCTTAACTTGCTAGCATGATGCTCATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAGAGTATTCTTTGCTGTATTGATACTTTGa      <  1:242708/141‑1 (MQ=12)
                                                                                                                                      ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  1:57802/141‑1 (MQ=32)
                                                                                                                                      ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  2:216593/141‑1 (MQ=32)
                                                                                                                                      ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  2:319597/141‑1 (MQ=32)
                                                                                                                                      ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAGTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      >  1:41949/1‑141 (MQ=17)
                                                                                                                                      ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTCAAGTATTCTTTGCTGTATTGATACTTTGa      >  2:298392/1‑141 (MQ=17)
                                                                                                                                      ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTgt      >  1:363729/1‑140 (MQ=17)
                                                                                                                                      ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  2:17873/141‑1 (MQ=17)
                                                                                                                                       tCGTTTGACTTGCTAGCATGCTGCTAATTTCGTGGCATGGGGACAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGGGGGGAAGTACGTTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGAt     <  1:339621/141‑1 (MQ=11)
                                                                                                                                       tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGCAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt     >  1:107856/1‑141 (MQ=12)
                                                                                                                                       tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCGTTATTGATACTTTgtt     >  2:335947/1‑139 (MQ=255)
                                                                                                                                       tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt     <  1:304553/141‑1 (MQ=32)
                                                                                                                                       tCGCTTAACTTGCTAGCATGATGCTAATGTCGTGGCATGGCGAACATCCGTAGACCTGAAGAGACCTGCGGTTCTTTTTCTATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt     >  1:247009/1‑141 (MQ=11)
                                                                                                                                        cGCTTAACTTGCTAGCATGATGCTAATTTCGTTGCATGGCGCAAATCCGTAGATGTGCAGAGACCTGCGGTTCTTTTTATATAGAGAGTAAAGACACTCAATACCTTTTAAAGTATTCTTTGATGTATTGATACTTTGCTa    >  2:181513/1‑141 (MQ=11)
                                                                                                                                         gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc   <  2:166783/141‑1 (MQ=17)
                                                                                                                                         gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc   <  2:235275/141‑1 (MQ=17)
                                                                                                                                          cttaccttacTAGCAGGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc  <  2:152016/141‑1 (MQ=255)
                                                                                                                                          cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc  <  1:77690/141‑1 (MQ=17)
                                                                                                                                          |                                                                                                                                             
CTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACC  >  CP000730/35738‑36016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: