BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,867	G→T	67.7%	intergenic (‑950/‑218)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,867	0	G	T	67.7%	131.0 / 102.5	124	intergenic (‑950/‑218)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (18/22); new base T (45/38); total (64/60)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

TAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTAC  >  CP000730/1929729‑1930000

tAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑Tg                                                                                                                                     <  1:374085/141‑1 (MQ=255)

        tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTAt                                                                                                                             >  1:324162/1‑141 (MQ=255)

          aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTCttt                                                                                                                           >  1:55246/1‑141 (MQ=255)

                                ttgtgataaGAAGCCAGTGGCTGTGCTGGTAGCTTTGCCGATGATATTAGAGTCGAGTCGTTTCTCTCTCAGGCGTTTATTTAGACGCAGCGAGAAG‑GGG‑TA‑CAC‑G‑T‑TGATGCTTATTTTCGTTCACATCATGGCACCAGt                                                                                                     <  2:137998/133‑1 (MQ=255)

                                        aGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTg                                                                                             >  2:338683/1‑141 (MQ=255)

                                         atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                           >  1:313169/15‑141 (MQ=255)

                                         atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                           <  1:20104/127‑1 (MQ=255)

                                         atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATAATAGCACCAGTCATCGGTGGc                                                                                           >  1:22756/15‑141 (MQ=255)

                                         atcggaagtagatatgCTTGTATAATCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                           >  2:271271/15‑141 (MQ=255)

                                          tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                          >  1:146370/14‑141 (MQ=255)

                                          aaGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                           <  1:41315/141‑1 (MQ=255)

                                          aaGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                           >  2:333846/1‑141 (MQ=255)

                                           cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                         >  2:362206/13‑141 (MQ=255)

                                           cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CAGTAC‑T‑T‑TGCTAATCAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                         <  2:191221/129‑1 (MQ=255)

                                           cggaagtagatatgCTTGGATATTCACCGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                         <  1:125923/129‑1 (MQ=255)

                                           cggaaatagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCGCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                         <  1:230718/129‑1 (MQ=255)

                                           aGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCGTAGCACCAGTCATCAGTGGCt                                                                                          >  1:136971/1‑141 (MQ=255)

                                            ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGAGTC‑AT‑TAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                        <  1:106383/130‑1 (MQ=255)

                                            ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                        <  1:309083/130‑1 (MQ=255)

                                            gCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                         >  1:382392/1‑141 (MQ=255)

                                             gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                       <  1:269895/131‑1 (MQ=255)

                                              aagtaggtatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                      <  2:77730/132‑1 (MQ=255)

                                                gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                    <  1:286635/134‑3 (MQ=255)

                                                gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                    <  1:245413/134‑3 (MQ=255)

                                                gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                    >  2:353669/8‑139 (MQ=255)

                                                gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTAGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                    >  1:341078/8‑139 (MQ=255)

                                                 tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                   <  1:350612/135‑4 (MQ=255)

                                                 tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATCGCACCAGTCATCAGTGGCTGTGctgt                                                                                   <  2:300355/135‑4 (MQ=255)

                                                 tGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGGCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCa                                                                                    <  2:332439/141‑1 (MQ=255)

                                                   gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                 <  2:29063/137‑6 (MQ=255)

                                                    atattcTTGTATATTCACAGAGGGTATAATAATAGATTCGCTTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGc                                                                                <  2:188222/136‑1 (MQ=255)

                                                     tgtgCTTTTATTTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                <  2:26293/141‑1 (MQ=255)

                                                     tatgCTTGTTGATTCACAGATGGTATAAAAATAGAGTCGCCTATCTCTCAGGCGTTAATTT‑‑ACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                               <  2:6360/139‑1 (MQ=255)

                                                      atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                              >  2:28660/2‑141 (MQ=255)

                                                      atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                              >  2:47848/2‑141 (MQ=255)

                                                        gCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                                                                            <  2:275243/141‑3 (MQ=255)

                                                          ttGTATATTTGCCGATGATATAATAGTATAGTTGCCTATCGCTTAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑T‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttt                                                                           <  1:107095/141‑1 (MQ=255)

                                                            gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                        <  1:177603/141‑7 (MQ=255)

                                                             tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGTCTGTGCCATTGCGttttttt                                                                        <  1:232460/141‑1 (MQ=255)

                                                                 ttcacagagggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑A‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                   >  2:264304/12‑141 (MQ=255)

                                                                   cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                 >  1:94314/5‑141 (MQ=255)

                                                                    acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                <  2:293779/138‑1 (MQ=255)

                                                                     cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                               >  2:47341/3‑141 (MQ=255)

                                                                      agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                              >  1:288208/2‑141 (MQ=255)

                                                                      agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                              >  2:57144/2‑141 (MQ=255)

                                                                       gatggtATAGTAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GGG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                             <  2:228541/141‑2 (MQ=255)

                                                                         tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTGCCT‑TCC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                           >  1:53099/4‑138 (MQ=255)

                                                                         tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                           >  1:378026/4‑141 (MQ=255)

                                                                         tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTaa                                                            >  2:102514/1‑141 (MQ=255)

                                                                         tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTaa                                                            >  2:362424/1‑141 (MQ=255)

                                                                          ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCACTT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                          >  1:264537/3‑141 (MQ=255)

                                                                          ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGTG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                          <  1:374042/139‑1 (MQ=255)

                                                                          ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCCGTGGCTGTGCTGTTGCGTATTATCCTTCTTGGCTTAGt                                                          >  2:56730/3‑141 (MQ=255)

                                                                          ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                          >  1:301275/3‑141 (MQ=255)

                                                                          ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                          >  2:236422/3‑141 (MQ=255)

                                                                          ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                          <  2:190065/139‑1 (MQ=255)

                                                                           gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                         >  2:115820/2‑141 (MQ=255)

                                                                           gtataATAATAGAGACGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                         >  1:200061/2‑141 (MQ=255)

                                                                            tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                        >  2:291491/1‑140 (MQ=255)

                                                                            tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                        >  2:320387/1‑140 (MQ=255)

                                                                            tataATAATAGAGTCGCCTATCTCTCAGGAGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                        <  1:258578/141‑2 (MQ=255)

                                                                              taataaTAGAGTCGCCTATGTCTCAGGCGTCAATT‑‑GACGAAGATAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc                                                      >  1:314965/1‑141 (MQ=255)

                                                                              taataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGa                                                       <  1:252728/141‑1 (MQ=255)

                                                                               aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                     >  1:118489/1‑141 (MQ=255)

                                                                                atatTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGGG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCTGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttactaaacgc                                                    <  2:269373/141‑11 (MQ=255)

                                                                                ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                    <  2:66599/137‑1 (MQ=255)

                                                                                agaagagagCCGCCTATCGCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑G‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                    <  1:267206/136‑1 (MQ=255)

                                                                                 taatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                   <  2:282803/138‑1 (MQ=255)

                                                                                 taatagAGTCGCCTATCTCTCAGGCGTCAAAT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                   <  1:127303/138‑1 (MQ=255)

                                                                                    tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                                <  2:15153/141‑3 (MQ=255)

                                                                                     agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                               >  2:64214/1‑138 (MQ=255)

                                                                                      gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                              <  1:289772/141‑5 (MQ=255)

                                                                                      gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGAAAACGCaacaaa                                              >  2:214494/1‑137 (MQ=255)

                                                                                       agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGGAAACGCaacaaat                                             >  2:353305/1‑136 (MQ=255)

                                                                                       agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                             <  1:52210/141‑6 (MQ=255)

                                                                                        gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                            >  2:143415/1‑135 (MQ=255)

                                                                                          cGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaaaa                                           <  1:103237/141‑1 (MQ=255)

                                                                                          cGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaaaa                                           >  1:153740/1‑141 (MQ=255)

                                                                                           gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                                          >  1:182836/1‑141 (MQ=255)

                                                                                           gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                                          <  1:258115/141‑1 (MQ=255)

                                                                                           gCCCATCTCTCAGGCGTCAATT‑‑GGCGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                         <  2:354581/141‑10 (MQ=255)

                                                                                             cTATCTCTCCAGCGTCAATTTAGAGGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGCTGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAg                                        >  1:280001/1‑141 (MQ=255)

                                                                                             cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                       <  1:241598/141‑12 (MQ=255)

                                                                                             cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                       >  1:223191/1‑130 (MQ=255)

                                                                                              tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                      >  1:35512/1‑129 (MQ=255)

                                                                                               aTCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGt                                      <  2:382392/141‑1 (MQ=255)

                                                                                               aTCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGAGGCTTATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTCGACGCAAGACAAACTactt                                      >  1:80999/1‑138 (MQ=255)

                                                                                               aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                     <  1:362206/141‑14 (MQ=255)

                                                                                               aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAG‑GTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                     >  1:144825/1‑128 (MQ=255)

                                                                                                tctctcAGGCGTCAATT‑‑GACGAAGGGAGGAG‑GTGCGT‑TAC‑T‑T‑TGCTACTCATTTTCGGTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATAGTTATTGCCTTAGTAAACGCaaccagtatttaattg                                    >  1:124039/1‑127 (MQ=255)

                                                                                                    ccAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTCATGGATGAGTATACGCAATACAAAATAGGTGACat                                 <  1:185038/140‑1 (MQ=255)

                                                                                                    ccAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACat                                 <  2:363207/140‑1 (MQ=255)

                                                                                                     cAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGTAATACAAAATAGGTGACata                                >  1:282712/1‑141 (MQ=255)

                                                                                                     cAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata                                <  2:321808/141‑1 (MQ=255)

                                                                                                             aaTTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa                        <  1:277385/141‑1 (MQ=255)

                                                                                                             aaTTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa                        <  1:218594/141‑1 (MQ=255)

                                                                                                                 tAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCaa                    <  1:68106/141‑1 (MQ=255)

                                                                                                                        agagagGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAAt             >  1:146014/1‑141 (MQ=255)

                                                                                                                            aggag‑gTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATcccc         >  1:82811/1‑141 (MQ=255)

                                                                                                                                g‑gTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCAc     <  1:102514/141‑1 (MQ=255)

TAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAG‑GTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTAC  >  CP000730/1929729‑1930000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`