Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,970 T→C 61.0% E194E (GAA→GAG USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,9700TC61.0% 285.0 / inf 436E194E (GAA→GAGUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base T (79/90);  new base C (123/140);  total (205/231)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01

Only 100 of 439 total aligned reads displayed.

CACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGC  >  CP000730/35851‑36110
                                                                                                                       |                                                                                                                                            
     atatATCATGTCAGCGTTCATTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGCCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGc                                                                                                                    >  1:210372/4‑141 (MQ=1)
                cAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAATAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                                                         <  2:285166/141‑1 (MQ=11)
                cAGTGTTCGCTTAACCTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                                                         <  1:134009/141‑1 (MQ=11)
                 cgctgtCGCCTGACTTGCTTGTATCATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCGTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                                                                                        <  2:154066/136‑1 (MQ=11)
                  gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                                                       >  1:335882/1‑141 (MQ=17)
                  gggtTCGTTTACTTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTACATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                                                       <  1:241656/139‑1 (MQ=11)
                   tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCATTTTATCCGAAGCTTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                                                                                      >  1:277676/1‑141 (MQ=11)
                     ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa                                                                                                    <  1:371890/141‑1 (MQ=255)
                     ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa                                                                                                    <  2:299028/141‑1 (MQ=255)
                     ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                                                    <  2:319597/141‑1 (MQ=32)
                      tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGCAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                                                   >  1:107856/1‑141 (MQ=12)
                      tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                                                   <  1:304553/141‑1 (MQ=32)
                      tCGCTTAACTTGCTAGCATGATGCTAATGTCGTGGCATGGCGAACATCCGTAGACCTGAAGAGACCTGCGGTTCTTTTTCTATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                                                   >  1:247009/1‑141 (MQ=11)
                       cGCTTAACTTGCTAGCATGATGCTAATTTCGTTGCATGGCGCAAATCCGTAGATGTGCAGAGACCTGCGGTTCTTTTTATATAGAGAGTAAAGACACTCAATACCTTTTAAAGTATTCTTTGATGTATTGATACTTTGCTa                                                                                                  >  2:181513/1‑141 (MQ=11)
                        gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                                 >  2:191193/3‑141 (MQ=17)
                         tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                                <  2:213354/140‑1 (MQ=17)
                            aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGTGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTg                                                                                             <  2:343035/141‑1 (MQ=11)
                            aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCttt                                                                                             >  1:273639/1‑140 (MQ=17)
                             acctGCTAGCACGATGCTAATTTCGTGGCATGGCGAACATCCGTAGCTCTGCCGCGAACTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                                                            <  1:79409/138‑1 (MQ=11)
                             aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGACAACTTCGCTGTATTGATACTTTGATACCTTGt                                                                                            <  1:91341/141‑1 (MQ=11)
                               ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                                                          <  2:131299/141‑1 (MQ=17)
                                tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                                                         <  2:53700/141‑1 (MQ=17)
                                 gCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtcttt                                                                                        >  1:315696/1‑141 (MQ=11)
                                  cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                       >  2:219680/1‑141 (MQ=17)
                                   tAGCATGATGCTAATATCGTGGCATGTCGAAAATACGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCATTACCTTTCAAAGTATTCTTTGCTGTATTGATCCTTTGATACCTTGtctttct                                                                                      <  1:232816/141‑1 (MQ=11)
                                     gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                                                    >  2:314249/1‑141 (MQ=17)
                                      cATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTGTAAAGTATTCTTTGATGTATTGATACTTTGATACCTTGTCGTTCTTAc                                                                                   >  1:50678/1‑141 (MQ=12)
                                      cATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTAc                                                                                   >  1:141545/1‑141 (MQ=17)
                                       atgatgCTAATTTCGTGGCATTGTGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACt                                                                                  <  2:363183/141‑1 (MQ=11)
                                        tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACGTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                                                 >  2:62959/1‑141 (MQ=11)
                                        tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                                                 >  1:223801/1‑141 (MQ=255)
                                         gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCCGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                                <  2:87911/141‑1 (MQ=21)
                                           tgCTAATTTCGTGGCATGGCGAAAATCCGTGCACCGGCACAATCCTTCTGGTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACATTGTCTTCCTTAATTTaa                                                                              <  2:268358/141‑1 (MQ=11)
                                            gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                                                                             >  2:100025/1‑141 (MQ=35)
                                            gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                                                                             <  2:89792/141‑1 (MQ=25)
                                            gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCAGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTCat                                                                             >  2:204530/1‑141 (MQ=255)
                                               aaTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATg                                                                          <  1:184506/141‑1 (MQ=255)
                                               aaTTTCGTGGCATGGCGCAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGACCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTCTTGATACTTCGCTACCTTGTCTCTCTTACTTTatgctg                                                                          >  2:303408/1‑136 (MQ=11)
                                               aaTTTCGTGGCATGGCGAAAAGCCGTAGATCGGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                          <  2:24452/141‑1 (MQ=255)
                                                agttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTATACTAATAAAGATCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGa                                                                         <  1:166101/139‑1 (MQ=11)
                                                  ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg                                                                       <  1:202657/141‑1 (MQ=37)
                                                  ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTATTACTTTAATATGACg                                                                       >  1:347503/1‑141 (MQ=25)
                                                     ttGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                    <  2:7799/140‑1 (MQ=255)
                                                     gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTTTATCGGGCGTAAATACCCTCAAGCACTCTGAATATTTTCTTTGCTGTATTGCTACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                    <  2:166/141‑1 (MQ=11)
                                                       ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                                                  >  2:241997/1‑141 (MQ=38)
                                                       ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACAGTAAt                                                                  >  1:196428/1‑141 (MQ=12)
                                                         cATGGGGAAAATCCGTAGATCCGAAGAGACCTACGGTTCTTTTTATATAGAGCATAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCt                                                                <  1:3098/141‑1 (MQ=14)
                                                           tGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                              <  1:120601/141‑1 (MQ=255)
                                                           tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                              <  1:604/141‑1 (MQ=255)
                                                             gggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTCTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                                                            <  1:258961/139‑1 (MQ=14)
                                                              cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTTCTTTAATATGACGGTGATCTTGCTc                                                           >  2:144408/1‑141 (MQ=255)
                                                                 aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                        <  2:27142/141‑1 (MQ=255)
                                                                  aaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                       <  1:161996/141‑1 (MQ=255)
                                                                     ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                    >  2:14981/1‑141 (MQ=37)
                                                                         aGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAt                                                <  1:101319/141‑1 (MQ=255)
                                                                           aTCTGCAGAGACCTGCGGTTCTTTTTATATAGAGCGTACATACACAGAATCCCCTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                                              <  1:124209/141‑1 (MQ=11)
                                                                           aTCTGATGACACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                                              <  2:381130/141‑1 (MQ=255)
                                                                             cTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                            <  1:155284/141‑1 (MQ=38)
                                                                               gAATAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGat                                          <  2:140469/141‑1 (MQ=255)
                                                                                aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata                                         <  2:20219/141‑1 (MQ=255)
                                                                                  gagaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                       <  1:266547/141‑1 (MQ=38)
                                                                                    gaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTc                                     <  2:115811/141‑1 (MQ=255)
                                                                                      ccTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTATTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCgt                                   >  1:254078/1‑140 (MQ=255)
                                                                                       ctacGGTTCTTTTTATATTGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt                                  <  1:170806/138‑1 (MQ=255)
                                                                                       cTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt                                  >  1:77028/1‑141 (MQ=255)
                                                                                       cTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt                                  >  1:10339/1‑141 (MQ=38)
                                                                                       cTGCGGTTCTTTTTATATAGAGCGTAAATACACGCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt                                  <  1:62946/141‑1 (MQ=255)
                                                                                        tGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATg                                 <  1:115322/141‑1 (MQ=255)
                                                                                         gCGGTTCTTTTTATATAGTGCGTAAATACACTCAATACCTTTTAACGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                <  2:226268/141‑1 (MQ=255)
                                                                                          cGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTGTTGCTTACTTTAAAGTGACGGCGCCCTTGCTCCCTGAGGTTCTTGAGATATTTCGATGTa                               >  2:326325/1‑141 (MQ=11)
                                                                                              tCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATATTGCTTAATGAGGTTATTTAGATATTTCGATGTACCAt                           >  2:257731/1‑141 (MQ=255)
                                                                                               cTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATTTTTCGATGTACAATg                          >  2:75594/1‑141 (MQ=255)
                                                                                                tttttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATgg                         <  1:342504/141‑2 (MQ=255)
                                                                                                tttttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATgg                         >  2:331802/1‑140 (MQ=255)
                                                                                                   ttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGCTGTACAATGGCAg                      >  1:351813/1‑141 (MQ=255)
                                                                                                    tacataGAGCTTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTCATATGACTGAGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGt                     <  2:306809/138‑1 (MQ=11)
                                                                                                      tataGAGCGTAAATACCTTCAATAACTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATCACTTGTCTTTCTTGCTTTAATATGACGGTGATCTTGCTCATTGAGGTTCTTCAGATATTTCGATGTACAATGACCGTCa                   >  2:122279/1‑141 (MQ=14)
                                                                                                          gagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtt               <  1:329035/141‑1 (MQ=255)
                                                                                                          gagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtt               >  1:28507/1‑141 (MQ=255)
                                                                                                          gagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACATTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGCTGTTTCGATGTACAATGGcaggcagggt               >  1:28306/1‑139 (MQ=14)
                                                                                                             cGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaa            <  2:351813/141‑1 (MQ=255)
                                                                                                              gTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttagg           >  1:67301/1‑139 (MQ=255)
                                                                                                               tAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagt          <  1:381651/141‑1 (MQ=255)
                                                                                                                aaaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtt         >  2:110616/1‑141 (MQ=255)
                                                                                                                aaaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtt         <  1:21814/141‑1 (MQ=255)
                                                                                                                 aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTAAAATGGCAGTCAGgtttaagttt        >  2:293986/1‑141 (MQ=255)
                                                                                                                 aaTACACTCAATACCGTTTAAAGTATTCTTTGCTGTACTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTAAATGCGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttacgttt        <  1:269579/141‑1 (MQ=14)
                                                                                                                  aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGTTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACTGTCTGgtttaagttta       >  1:174520/1‑141 (MQ=14)
                                                                                                                  aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta       >  1:292070/1‑141 (MQ=255)
                                                                                                                  aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTAAGgtttaatttta       >  2:45907/1‑141 (MQ=255)
                                                                                                                   ttcATTCAATACCTTTTAAAGTATTCTTTGTCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACTGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa      <  1:38870/139‑1 (MQ=255)
                                                                                                                   tACATTCAATACCTTTTAAGGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa      >  1:228553/1‑141 (MQ=255)
                                                                                                                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGGTACTTTGATACCTGGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATGTTTCGATGTACAATGGCAGTCAGgtttaagtttaa      >  2:348543/1‑141 (MQ=14)
                                                                                                                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGGTGTACAATGGCAGTCAGgtttaagtttaa      >  1:294142/1‑141 (MQ=255)
                                                                                                                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa      >  2:107354/1‑141 (MQ=255)
                                                                                                                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACATTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa      >  2:280760/1‑141 (MQ=255)
                                                                                                                    actttCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTaaa     <  2:173588/138‑1 (MQ=255)
                                                                                                                     cATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTaaaa    >  1:4239/1‑141 (MQ=255)
                                                                                                                       ttCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGc  >  1:332813/1‑141 (MQ=255)
                                                                                                                       ttCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGc  <  2:357674/141‑1 (MQ=255)
                                                                                                                       |                                                                                                                                            
CACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGC  >  CP000730/35851‑36110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: