Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,532,2980AT100.0% 85.9 / ‑6.5 30T15T (ACA→ACTUSA300HOU_2392FemAB family peptidoglycan biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (7/21);  minor base G (0/1);  total (7/23)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACG  >  CP000730/2532161‑2532435
                                                                                                                                         |                                                                                                                                         
tgtgTATTTGTAATCAGATTTTGATAAAATAAAAAAATATCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGTGTGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAAAGCTTTTactaca                                                                                                                                        >  2:181863/1‑141 (MQ=255)
          tAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAAGAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttttt                                                                                                                              <  1:142012/141‑1 (MQ=255)
            aTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTca                                                                                                                            >  1:156427/1‑141 (MQ=255)
            aTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTca                                                                                                                            <  2:240458/141‑1 (MQ=255)
               aatttttGATAAAATAAAAAAATAGCATTATTAATAACTACATAAAGTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAt                                                                                                                         <  1:164548/139‑1 (MQ=255)
                    ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                                    <  1:275535/141‑1 (MQ=255)
                    ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACCTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                                    <  1:73945/141‑1 (MQ=255)
                      gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacacac                                                                                                                  >  1:306830/1‑141 (MQ=255)
                          aaataaaaAAAAAGCATCATTAATAAATTCAAAAATTATATCTCAATTCAGAGGGGGATGGGATAAAATGAACTTTGTAACTTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCATATTACACACAATc                                                                                                              <  2:71479/141‑1 (MQ=255)
                             taaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGc                                                                                                           <  1:148298/141‑1 (MQ=255)
                                   aaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCa                                                                                                     <  1:55076/141‑1 (MQ=255)
                                                ataaATTCAAAAATTATAGTTCCATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAATCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATaga                                                                                        <  2:322545/141‑1 (MQ=255)
                                                ataaATTCAAAAATTATAGTTCAATTCAGAGAGCGATGGGATAAAATGAACTTCGGAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACATAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATaga                                                                                        <  2:210174/141‑1 (MQ=255)
                                                                          catagagGGCTGGGATAAACTGCACTTTTTAAAGTTGACTTCGGATAAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAACCCGCGATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt                                                              <  2:308078/138‑1 (MQ=255)
                                                                          cAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt                                                              <  1:343720/141‑1 (MQ=255)
                                                                            gagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACCCAATCAGCTATTCATTACAATCATCGAGTTGATTTACAAGGCGATCTGAAtttcg                                                            >  2:245745/1‑137 (MQ=255)
                                                                                gggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgg                                                        <  1:213997/141‑1 (MQ=255)
                                                                                 ggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAggg                                                       <  2:156427/141‑1 (MQ=255)
                                                                                               taaCTTTGTAACGTTGACTACGGATGAGTTTAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACaa                                         <  2:342048/140‑1 (MQ=255)
                                                                                                        aaCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAgg                                >  2:217972/1‑140 (MQ=255)
                                                                                                        aaCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGAAAATGGTCAAGt                                >  2:218016/1‑141 (MQ=255)
                                                                                                           gTTGACTTGGGTTGAGGTCAATGCGTTTACTACAAAGCATTTTTCACATGACACACAAACGGCTATGCATTACAATCAGAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAt                             <  1:181863/141‑1 (MQ=255)
                                                                                                            gtcaCTTCGTATGAGTTCAATGCCTTTACTACAAAGCATCTTTCACATTACACACAATCAGCTATTCATTACAACCATAGAGTTGAGTTAAAAGGCGATGTGCATCTTGTAGGGGCTAAAGATGACAATGGTCAAGTGAtt                            <  1:46014/138‑1 (MQ=255)
                                                                                                               aCTTCGGATGAGTTCAATGCGTTTACGACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCa                         <  1:159334/141‑1 (MQ=255)
                                                                                                                    ggATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGGTg                    >  2:4008/1‑141 (MQ=255)
                                                                                                                            tCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGGGTTGATTTAAAAGGCGATGTGTATCTTGTAGTGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGa            <  2:306830/141‑1 (MQ=255)
                                                                                                                              aaTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACa          >  2:212883/1‑141 (MQ=255)
                                                                                                                               aTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTTATTACAATCTTAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAg         <  1:106298/141‑1 (MQ=255)
                                                                                                                                     ttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACACTCCTTAATTGGATTTAAACGGCGATGGGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcac   <  1:72395/141‑1 (MQ=255)
                                                                                                                                     ttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcac   <  1:289356/141‑1 (MQ=255)
                                                                                                                                      tACCACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGCTGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcacg  <  1:168270/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACG  >  CP000730/2532161‑2532435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: