Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,416,4580AT100.0% 57.2 / NA 21I216N (ATT→AAT) USA300HOU_1314oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/7);  total (14/7)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACT  >  CP000730/1416331‑1416589
                                                                                                                               |                                                                                                                                   
aTCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAat                                                                                                                         >  2:183127/1‑140 (MQ=255)
 tCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTCCTGACAata                                                                                                                        >  1:300833/1‑140 (MQ=255)
     aGTAATCCCTCTTTAATTGTTTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTc                                                                                                                    <  2:184374/140‑1 (MQ=255)
      gTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCa                                                                                                                   >  1:24617/1‑140 (MQ=255)
             ctctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACAtt                                                                                                            >  2:326998/1‑139 (MQ=255)
                 ttAATTGGGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCACGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACtt                                                                                                        <  2:214072/140‑1 (MQ=255)
                     ttGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCCTACTTGAAATGATTAGTATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTCCTGACAATATTTCAATACATCACTTAAGt                                                                                                    >  1:30316/1‑140 (MQ=255)
                         gtTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtg                                                                                                >  1:18966/1‑140 (MQ=255)
                                   tAATTAGACGAACAGTATACGGATGCGACTTCTCATACTTGAAATGATTAATATTACGTCGTTCAATGATTTGACCTTCTTTTAGCACATACTTGTCCTGACAATATTGCCATACATGCCTTAAGTCAAGTGAGataatg                                                                                      >  2:85278/1‑139 (MQ=255)
                                                                            aaaTGATTAATATTACCTCGTTCAATGGTTTGACCTTCTATTAAAACATAATTGTACTGACAATATTTCAATACATGACTTACGTTCTGTGTGATCATAACTGATGTTTAACCATGTTCTAATACAATATACTGtaataa                                             >  1:29846/1‑140 (MQ=255)
                                                                            aaaTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGCACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGtaataa                                             >  1:236844/1‑140 (MQ=255)
                                                                              aTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAAt                                           <  2:82880/140‑1 (MQ=255)
                                                                                     atatTACCTCGTTCAATGATTTGAACTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCAc                                    <  1:228754/140‑1 (MQ=255)
                                                                                     atatTACCTCGTTCAATGAATTGACCTGCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATAAAATATGCTGTAATCAATCCATCAc                                    >  1:262614/1‑140 (MQ=255)
                                                                                            cTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTa                             <  1:271525/140‑1 (MQ=255)
                                                                                              cGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAGTACAATATGCTGTAATAAAGCCATAACTTGATTAcc                           >  2:236412/1‑140 (MQ=255)
                                                                                                 tCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGtt                        >  2:188999/1‑140 (MQ=255)
                                                                                                  cAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTc                       <  2:59627/140‑1 (MQ=255)
                                                                                                     tGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATCCTGTAATAAATACATAACTTGATTACAGTTCaaa                    >  1:132660/1‑140 (MQ=255)
                                                                                                         ttGACTCTCTTTTAAAACATAATTTTACTGACAATATTTCAATACATTACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCAt                <  2:197780/140‑1 (MQ=255)
                                                                                                                       aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACt  >  1:35973/1‑140 (MQ=255)
                                                                                                                               |                                                                                                                                   
ATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACT  >  CP000730/1416331‑1416589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: