Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,021,817 C→T 100% V139I (GTT→ATT)  USA300HOU_1873 ← acyl‑CoA hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,021,8170CT96.3% 111.3 / ‑4.8 40V139I (GTT→ATT) USA300HOU_1873acyl‑CoA hydrolase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (17/21);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCACT  >  CP000730/2021682‑2021953
                                                                                                                                       |                                                                                                                                          
aCAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAAtatca                                                                                                                                       <  1:272247/140‑1 (MQ=255)
 cAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAAtatcat                                                                                                                                      <  2:246397/140‑1 (MQ=255)
        tCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTgg                                                                                                                              <  1:268808/140‑1 (MQ=255)
        tCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTAATGGTTCTGTATCATAAAACCATTTTTCAATATCAACTTCTgg                                                                                                                              >  1:9152/1‑140 (MQ=255)
         cTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTCGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGa                                                                                                                             >  1:203029/1‑140 (MQ=255)
         cTTTATATTTTTAGTCCCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGa                                                                                                                             <  2:217292/140‑1 (MQ=255)
          tttATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGat                                                                                                                            >  2:310212/1‑140 (MQ=255)
          tttATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGat                                                                                                                            >  2:310112/1‑140 (MQ=255)
             atatTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGAtata                                                                                                                         <  1:138596/140‑1 (MQ=255)
                      ggctctAATAGGTTGCACCTGAGCGAAATATTCAATTGTTTGTTTTCTTGCACTTCCACATTCTTTTCTTCGTCTAACACGTTGTGGGTCTGTATCATAAAACCATTTTGCAATATCATCTTCTGGATATACACCAGTTa                                                                                                                <  2:83602/138‑1 (MQ=255)
                      gTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCAGCTTCTGGATATACACCTGGTa                                                                                                                >  2:272593/1‑140 (MQ=255)
                            aaTATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGtt                                                                                                          >  1:337021/1‑140 (MQ=255)
                               atGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGAAATACACCAGGTACATgataag                                                                                                       >  1:115871/1‑135 (MQ=255)
                                tGTTGCACTTTAGCTAAATATTCAATTGTTTGATTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAgg                                                                                                      <  1:93947/140‑1 (MQ=255)
                                   ctcACGTGATCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCACCTTCTGGATATACACCAGGTACATGTTTAGGttt                                                                                                   <  2:35848/138‑1 (MQ=255)
                                         tGAGCTAAATATTCAATTGTTAGCTCCCATTCAATGCGACGTGCTTTTCTTAGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTtc                                                                                             >  2:252940/1‑140 (MQ=255)
                                              tAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTcatcat                                                                                        <  1:344777/140‑1 (MQ=255)
                                                   atTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAAc                                                                                   <  1:310212/140‑1 (MQ=255)
                                                   atTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAAc                                                                                   <  1:310112/140‑1 (MQ=255)
                                                    ttCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACg                                                                                  <  1:338160/140‑1 (MQ=255)
                                                      cAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGca                                                                                >  2:35205/1‑140 (MQ=255)
                                                        aTTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGcaac                                                                              <  1:226616/140‑1 (MQ=255)
                                                        aTTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTT‑‑GCGTCGGTCTCCTTGGATCTAAATCAGGTTCATGTTTAGTTTTACCTTCATCATCTAGCgtgac                                                                              >  1:82316/1‑136 (MQ=255)
                                                         ttgtttgtTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATATTTAGGTTTGCCTTCATCATCTAACGcaaca                                                                             >  1:285511/1‑140 (MQ=255)
                                                                tttACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTAGTGGTGCTGTATCATAAAACCATTTTTCAATATAATCTTCTGGATATACACAAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACCAATGTTa                                                                      >  2:157577/1‑140 (MQ=255)
                                                                    cTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCCACAACTGTTAAGtc                                                                  >  1:22411/1‑139 (MQ=255)
                                                                      ttaaaTGCGACGTGCTTTTCTTCGTTAAACACGGTGTGGTGCTGTTTGATAGAACCGTGTGTAAATATCGTCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAAc                                                                <  1:154547/137‑1 (MQ=255)
                                                                      ttCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATTTCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTATCGCAACAAATGTTGAGTAAc                                                                >  1:79160/1‑140 (MQ=255)
                                                                               aCGTGCTTTTCTTCGTTCAACACGTTTTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAg                                                       <  1:312136/140‑1 (MQ=255)
                                                                                   gCTTTTCTTCGTTCAACACGTTGTGGTGCCGTATCAGAAAACCAGTTTGCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTaa                                                   <  2:187832/140‑1 (MQ=255)
                                                                                      tttCTTCGTTCAACACGTTGTGGGGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATc                                                <  2:203029/140‑1 (MQ=255)
                                                                                          ctcGTTTCACACGTTGTGGTGCTGGAGCATAAAGCCATGTTTCCATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGc                                            <  2:320790/139‑1 (MQ=255)
                                                                                            cGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCtt                                          <  1:35205/140‑1 (MQ=255)
                                                                                                cAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATGATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCACCAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGtta                                      >  1:231812/1‑140 (MQ=255)
                                                                                                   cacGTTGTGTGGCCGCATCAGAAAACCATTTGTCCACATCATCTTCTGAATATACACCAGGCACATGTTTACGTTCTCCTTCCTCATCTAACGCAACAAATGTCTACTAACTTGTCGCAATTCAATCATGCGTGttaccc                                   <  2:4981/140‑4 (MQ=255)
                                                                                                   cacGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGAAACTAAATGCATCTAAATCATGATTGttatta                                   >  1:1269/1‑140 (MQ=255)
                                                                                                     cGTTCTGGTTCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTTATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTaaa                                 <  1:21773/140‑1 (MQ=255)
                                                                                                         gtggtgCTGTATCATAAAACCATTTTTCAATATCATCCTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCAACTAACGCAACAAATGTTAAGTAACTTAACGCAGCTAAATCATGCTTGTTATTAAATACa                             <  2:22139/140‑1 (MQ=255)
                                                                                                                          aaCCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGAAGCTAAATCATGCTTGTTATTAAATACATCGTAAATTCTAAtttt            >  1:272397/1‑139 (MQ=255)
                                                                                                                           aCCATTTTTTATTTTCATTTTCTGGATATACACCAGGTACATGTTTAGGTTTGCTTTCATCATCTAACGCAACAAATGTTAAGTAACTTATTGCAGCTAAATCATGTTTTTTCTTCATTACCTCGTCAATTCTAttttta           >  2:150029/1‑138 (MQ=255)
                                                                                                                                 tttCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAACGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGAcc     <  1:286726/140‑1 (MQ=255)
                                                                                                                                    cAATATCATCTTTTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCACt  <  1:102545/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                          
ACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCACT  >  CP000730/2021682‑2021953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: