| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,021,817 | C→T | 100% | V139I (GTT→ATT) | USA300HOU_1873 ← | acyl‑CoA hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,021,817 | 0 | C | T | 96.3% | 111.3 / ‑4.8 | 40 | V139I (GTT→ATT) | USA300HOU_1873 | acyl‑CoA hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (17/21); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCACT > CP000730/2021682‑2021953 | aCAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAAtatc‑a < 1:272247/140‑1 (MQ=255) cAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAAtatc‑at < 2:246397/140‑1 (MQ=255) tCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑Tgg < 1:268808/140‑1 (MQ=255) tCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTAATGGTTCTGTATCATAAAACCATTTTTCAATATC‑AACTTC‑Tgg > 1:9152/1‑140 (MQ=255) cTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTCGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGa > 1:203029/1‑140 (MQ=255) cTTTATATTTTTAGTCCCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGa < 2:217292/140‑1 (MQ=255) tttATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGat > 2:310212/1‑140 (MQ=255) tttATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGat > 2:310112/1‑140 (MQ=255) atatTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGAtata < 1:138596/140‑1 (MQ=255) ggctctAATAGGTTGCACCTGAGCGAAATATTCAATTGTTTGTTTTCTTGCACTTCCACATTCTTTTCTTCGTCTAACACGTTGTGGGTCTGTATCATAAAACCATTTTGCAATATC‑ATCTTC‑TGGATATACACCAGTTa < 2:83602/138‑1 (MQ=255) gTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑AGCTTC‑TGGATATACACCTGGTa > 2:272593/1‑140 (MQ=255) aaTATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGtt > 1:337021/1‑140 (MQ=255) atGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGAAATACACCAGGTACATgataag > 1:115871/1‑135 (MQ=255) tGTTGCACTTTAGCTAAATATTCAATTGTTTGATTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAgg < 1:93947/140‑1 (MQ=255) ctcACGTGATCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ACCTTC‑TGGATATACACCAGGTACATGTTTAGGttt < 2:35848/138‑1 (MQ=255) tGAGCTAAATATTCAATTGTTAGCTCCCATTCAATGCGACGTGCTTTTCTTAGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTtc > 2:252940/1‑140 (MQ=255) tAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTcatcat < 1:344777/140‑1 (MQ=255) atTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAAc < 1:310212/140‑1 (MQ=255) atTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAAc < 1:310112/140‑1 (MQ=255) ttCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACg < 1:338160/140‑1 (MQ=255) cAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGca > 2:35205/1‑140 (MQ=255) aTTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGcaac < 1:226616/140‑1 (MQ=255) aTTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTT‑‑GCGTCGGTCTCCTTGGATCTAAATCAGGTTCATGTTTAGTTTTACCTTCATCATCTAGCgtgac > 1:82316/1‑136 (MQ=255) ttgtttgtTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATATTTAGGTTTGCCTTCATCATCTAACGcaaca > 1:285511/1‑140 (MQ=255) tttACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTAGTGGTGCTGTATCATAAAACCATTTTTCAATATA‑ATCTTC‑TGGATATACACAAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACCAATGTTa > 2:157577/1‑140 (MQ=255) cTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCCACAACTGTTAAGtc > 1:22411/1‑139 (MQ=255) ttaaaTGCGACGTGCTTTTCTTCGTTAAACACGGTGTGGTGCTGTTTGATAGAACCGTGTGTAAATATC‑GTCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAAc < 1:154547/137‑1 (MQ=255) ttCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATTTC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTATCGCAACAAATGTTGAGTAAc > 1:79160/1‑140 (MQ=255) aCGTGCTTTTCTTCGTTCAACACGTTTTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAg < 1:312136/140‑1 (MQ=255) gCTTTTCTTCGTTCAACACGTTGTGGTGCCGTATCAGAAAACCAGTTTGCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTaa < 2:187832/140‑1 (MQ=255) tttCTTCGTTCAACACGTTGTGGGGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATc < 2:203029/140‑1 (MQ=255) ctcGTTTCACACGTTGTGGTGCTGGAGCATAAAGCCATGTTTCCATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGc < 2:320790/139‑1 (MQ=255) cGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCtt < 1:35205/140‑1 (MQ=255) cAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATG‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCACCAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGtta > 1:231812/1‑140 (MQ=255) cacGTTGTGTGGCCGCATCAGAAAACCATTTGTCCACATC‑ATCTTC‑TGAATATACACCAGGCACATGTTTACGTTCTCCTTCCTCATCTAACGCAACAAATGTCTACTAACTTGTCGCAATTCAATCATGCGTGttaccc < 2:4981/140‑4 (MQ=255) cacGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGAAACTAAATGCATCTAAATCATGATTGttatta > 1:1269/1‑140 (MQ=255) cGTTCTGGTTCTGTATCATAAAACCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTTATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTaaa < 1:21773/140‑1 (MQ=255) gtggtgCTGTATCATAAAACCATTTTTCAATATC‑ATCCTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCAACTAACGCAACAAATGTTAAGTAACTTAACGCAGCTAAATCATGCTTGTTATTAAATACa < 2:22139/140‑1 (MQ=255) aaCCATTTTTCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGAAGCTAAATCATGCTTGTTATTAAATACATCGTAAATTCTAAtttt > 1:272397/1‑139 (MQ=255) aCCATTTTTTATTTTC‑ATTTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCTTTCATCATCTAACGCAACAAATGTTAAGTAACTTATTGCAGCTAAATCATGTTTTTTCTTCATTACCTCGTCAATTCTAttttta > 2:150029/1‑138 (MQ=255) tttCAATATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAACGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGAcc < 1:286726/140‑1 (MQ=255) cAATATC‑ATCTTT‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCACt < 1:102545/140‑1 (MQ=255) | ACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATC‑ATCTTC‑TGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCACT > CP000730/2021682‑2021953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |