Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,279,7050GA100.0% 119.8 / NA 41intergenic (+1373/+6659)mtlD/glmMmannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/21);  total (20/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCT  >  CP000730/2279570‑2279839
                                                                                                                                       |                                                                                                                                      
gtgCCGTTATTAGTTGATTCTTCTTCTGGTTTACTAGTTACGTCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgt                                                                                                                                    <  1:42796/140‑1 (MQ=255)
gtgCCGGTATTAGTTGATTCTAGCTCTGGTTTACTAGTTCCACCGTTATTCATAAACGGTCAATTGGGTGATGCAGCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGGTGATGTTACtgt                                                                                                                                    <  1:350784/140‑1 (MQ=255)
      ttattaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATGGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTCTTTc                                                                                                                              >  2:187667/1‑140 (MQ=255)
           tgTTTATTCTACTTCTGGTTTACTAGTTACATCTTTATCCATTGTCGGACTGCTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTg                                                                                                                         <  1:81789/139‑1 (MQ=255)
           aGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGGCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTg                                                                                                                         <  2:314164/140‑1 (MQ=255)
              tggttCTACGTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATTTACACTAGGATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTg                                                                                                                      <  2:172797/137‑1 (MQ=255)
              tGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCCCCTGTTg                                                                                                                      >  2:14929/1‑140 (MQ=255)
                     aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGGTTCACCTGTTGCCGCATc                                                                                                               >  2:283200/1‑140 (MQ=255)
                      cTTCTGGGTGACTGGTTACCTCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGATATTAGCTTGCGGTTTAGCATTTGCATCATCCGTTTCTGATGATACTATAGTTGCACCTGTTGCTGCAttt                                                                                                              >  2:193104/1‑138 (MQ=255)
                         cTGGTTTATTCGTTACCGCGTTATTCATTGTCGGACTGTTTGGTGATGCATCTTCACTAGAATTTTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACta                                                                                                           <  2:336369/140‑1 (MQ=255)
                          tGGTTTACGAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGTTGAATATATACTAGGATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtat                                                                                                          <  2:126820/140‑1 (MQ=255)
                                aCTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTTTTTg                                                                                                    >  2:324579/1‑140 (MQ=255)
                                aCTAGTTACATCGTTATCCAATGTCGATTTGCTTCTGTAGGCTACTGCACAAGAATTGTTATTAGCTTGCCGTTTAGCATTTGCATCAACAGACTCTGATGGTACTGTTATTTCTCCTGTTGCCGCATATCTataattag                                                                                                    >  2:147939/1‑138 (MQ=255)
                                   aGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgtg                                                                                                 <  1:314982/140‑1 (MQ=255)
                                            gTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATTAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgag                                                                                        <  2:340864/140‑1 (MQ=255)
                                                  cATTGTCGGACTGTTTGTTGATGCATATACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATATTACTGTAGTGTCACCTTTTGCCGCATCCCTATTAATTGGTGTTGTCGGAGCAGGGt                                                                                  >  2:10240/1‑140 (MQ=255)
                                                    ttGTCGGACTGTTTGTTGATACATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGAGTTGTCGGAGAAGCGTCt                                                                                >  1:15436/1‑140 (MQ=255)
                                                     tGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTg                                                                               <  1:285937/140‑1 (MQ=255)
                                                       tCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCt                                                                             <  1:111957/140‑1 (MQ=255)
                                                       tCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCt                                                                             <  1:314998/140‑1 (MQ=255)
                                                        cGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCtt                                                                            >  1:309992/1‑140 (MQ=255)
                                                             tgtttgttGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGGATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCa                                                                       >  1:277604/1‑140 (MQ=255)
                                                             tgtttgttGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCa                                                                       <  2:24414/140‑1 (MQ=255)
                                                               tttgttGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCAtt                                                                     >  2:240202/1‑140 (MQ=255)
                                                                  gttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGc                                                                  <  1:88052/140‑1 (MQ=255)
                                                                    tgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCGTTGCCATTCGCTg                                                                >  2:34065/1‑140 (MQ=255)
                                                                        gCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCCGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGTTGTTTTCGGATAAGCGTCTGCTTTTCCCTTAGCTgtcgt                                                            >  1:133508/1‑140 (MQ=255)
                                                                        gCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcgt                                                            >  2:335542/1‑140 (MQ=255)
                                                                                    gAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGtt                                                <  2:218052/140‑1 (MQ=255)
                                                                                     aaTTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGGCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTa                                               <  1:245502/140‑1 (MQ=255)
                                                                                      aTTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAg                                              <  1:301539/140‑1 (MQ=255)
                                                                                          ttattaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCAGTAGCTGTCGTGTCCGGTACGTTAGGGTg                                          >  2:73741/1‑140 (MQ=255)
                                                                                                 cTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTa                                   <  1:239175/140‑1 (MQ=255)
                                                                                                      ggTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTc                              <  1:306532/140‑1 (MQ=255)
                                                                                                        tttATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCCCTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg                            >  1:248761/1‑140 (MQ=255)
                                                                                                        tttATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg                            >  2:319293/1‑140 (MQ=255)
                                                                                                                  gCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCAtt                  >  2:324904/1‑140 (MQ=255)
                                                                                                                       atcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAt             <  2:183646/140‑1 (MQ=255)
                                                                                                                         caGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAttt           >  1:39015/1‑140 (MQ=255)
                                                                                                                               cTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAttt     >  1:28113/1‑140 (MQ=255)
                                                                                                                                  aTGTTACTGTTGTTTCACCTTTCGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTATCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCt  <  2:221593/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCT  >  CP000730/2279570‑2279839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: