| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,856,087 | T→A | 100% | P80P (CCT→CCA) | USA300HOU_2698 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,856,087 | 0 | T | A | 100.0% | 92.6 / ‑6.5 | 33 | P80P (CCT→CCA) | USA300HOU_2698 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (15/16); minor base C (0/1); total (16/17) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCTAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAATT > CP000730/2855952‑2856222 | gaTGTATGGCGTTTGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAAc < 1:118336/140‑1 (MQ=255) gCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGACAAAACGTc > 2:335879/1‑140 (MQ=255) cATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTca > 1:355407/1‑140 (MQ=255) gTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCACAACGTCAACaaaa > 1:162997/1‑139 (MQ=255) tGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGa > 2:350701/1‑140 (MQ=255) cTAAAGTTATTTCAATATTGGAACTAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGAt < 1:105441/140‑1 (MQ=255) tataTCAATATTGGAACAAGATTTATTTCAGAACCAACTAAATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAAAAGGGGATGTGTCATGTAGTCATGCGCTGGTCCGTTAACAAATGATGGTTTTTGTAg > 2:334422/1‑140 (MQ=255) tatCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAgtt > 1:225186/1‑140 (MQ=255) cAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAgttgtt > 2:325703/1‑140 (MQ=255) atatTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGACGTTCAGTAATCCTTCCCTTTTAAGTTAACCGGAGATGTGTCATGCAATCATGCGCAAACACATCTACAAATGAGAGATTTTATAGTTGTTAc > 2:336715/1‑140 (MQ=255) atTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCTGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTAcag < 2:188035/140‑1 (MQ=255) ttGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTAcagc < 1:165832/140‑1 (MQ=255) cAAGCTATACTTCAACATCAACTGCATTTTAAAACGGAAGCTCAGTCATCATTACCTTCTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCAtt > 2:254701/1‑140 (MQ=255) aTTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTg < 1:907/140‑1 (MQ=255) cAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAACGAGAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACg < 1:335879/140‑1 (MQ=255) gTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAatg > 2:125252/1‑139 (MQ=255) ttcatcaTTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAatttattt < 1:308204/139‑1 (MQ=255) tcatTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTc < 1:189476/140‑1 (MQ=255) ccTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTccc < 2:354778/140‑1 (MQ=255) tttagacaaCAGTGGATGTGTCATGCAATCATGCGCCAAAACGTCAACAACTCACAGATCTGACAGGGGTGACAGCATCAGTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTAGTTGGTCTGTCCCTGGc < 2:214857/133‑1 (MQ=255) ttACGTTAACATGAGAGGTGTCATGCAGTCAGGCGCCCATACGTCAACAAATGATATATTTTATCGTTGTTACATCAGCACGAGACACTAAATTATTACCATCACTGGTTACAACAGAATTTATTTTGTCAGTccttagc < 2:160192/140‑6 (MQ=255) tAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGcc < 2:237843/140‑1 (MQ=255) tAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGcc < 2:158581/140‑1 (MQ=255) ttAACAGGGGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCtt < 1:144387/140‑1 (MQ=255) aaCAGGAGATGTGTCATGCAATCATTCGCCAAAACGTCAACAAATGATAGATTTTATGGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTg > 2:57290/1‑140 (MQ=255) aaCAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTAGAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTg > 2:286501/1‑140 (MQ=255) aCAGGAGATGTGTCATGCAATCATGCGCCAAAGCGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGa < 2:309377/140‑1 (MQ=255) cAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGAc < 2:355407/140‑1 (MQ=255) gtCATACAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGAc > 1:342134/1‑140 (MQ=255) gCAATCATGCGCCATAACGTCAACAAATGGTAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCaa > 1:190484/1‑140 (MQ=255) aTCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCACAATTATATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAata < 1:334422/140‑1 (MQ=255) cATGCGACAGAACGTCAACAAATAATCGTCAGTATAGTTGATACAGCAGCATTAGATATTGAATTAGTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCATTGACATATACGGACCTCAAATAtt > 1:262088/1‑140 (MQ=255) cgcCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAAtt > 1:197300/1‑140 (MQ=255) | GATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCTAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAATT > CP000730/2855952‑2856222 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |