| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,864,675 | G→T | 13.7% | intergenic (+439/+1110) | USA300HOU_2709 → / ← USA300HOU_2710 | hypothetical protein/hypothetical membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,864,675 | 0 | G | T | 13.7% | 73.5 / 2.7 | 29 | intergenic (+439/+1110) | USA300HOU_2709/USA300HOU_2710 | hypothetical protein/hypothetical membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (17/8); new base T (2/2); total (19/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
ATTGTAGTGTATTTGTTTAGAATATCCTCTTTTTTAGTTATGAATTTGTTACAAATATTAAGTGCAAAAGCACACGGAGGTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTAGCGATGGCTACAAAACTTTAGAAGAAGGTCAAAAAGTTACTTTCGAAATCACTGAAGGTCAACGTGGAGACCAAGCAG > CP000730/2864541‑2864809 | aTTGTAGTGTATTTGTTTAGAATATCCTCTTTTTTAGTTATGAATTTGTTACAAATATTAAGTGCAAAAGCACACGGAGGTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCa > 1:240073/1‑140 (MQ=255) tGTAGTGTATTTGTTTAGAATATCCTTTTTTTTAGTTATGAATTTGTTACAAATATTAAGTGCAAAAGCACACGGAGGTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGTTTTCATc < 2:75760/140‑1 (MQ=255) gcttAGAATATCCTCTTTTTTAGTTATGAATTTGTTACAAATATTCAGTGCAAAATCACACGGAGGTTTTCTATATGAATAACTGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAAt < 2:324093/138‑1 (MQ=255) gTTTAGAATATCCTCTTTTTTAGTTATGAATTTGTTACAAATATTAAGTGCAAAAGCACACGGAGGTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAAt < 1:220078/140‑1 (MQ=255) aatagtGAATTTGTTAAAAATATTAATTGCAAAAGCACACGGAGGTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACAt < 1:132042/135‑1 (MQ=255) gTTATGAATTTGTTACAAATATTAAGTGCAAAAGCACACGGAGGTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACAtt > 2:237553/1‑140 (MQ=255) tGTTACAAATATTAAGTGCAAAAGCAGACGGAGGTTTTCTATATGAATAACGGTAGAGTAAGATGGTTTAACGCAGAAAAAGGAATTGGAATAATCGAACAAGAAAATGGCGGAAAAGTATTCGTACAGTTCTCAGGGAt > 2:226644/1‑140 (MQ=255) cAAATATTAAGTGCAAAAGCACACGGAGGTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATAGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTa > 1:2996/1‑140 (MQ=255) aaTATTAAGTGCAAAAGCACACGGAGGTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTAGc > 1:101798/1‑140 (MQ=255) aGTGCAAAAGCACACGGAGTTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTAGCGATGGCt < 2:240073/140‑1 (MQ=255) aGTGCAAAAGCACACGGAGGTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTAGCGATGGCt < 2:47863/140‑1 (MQ=255) gTGCAAAAGCACACGGAGGTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCACTAGCGATGGCTa > 1:341115/1‑140 (MQ=255) gCACACGGAGGTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTAGCGATGGCTACAAAACtt < 2:113332/140‑1 (MQ=255) cacaCGGAGGTTTTCTATATGAATACGGGCACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTAGCGATGGCTACAAAACttt > 1:353700/1‑140 (MQ=255) cGGAGGTTTTCTATATGCCGTCCGAGACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGTTTTCATCGAACAAGAAAATGGCGGAAACGTATTCGTACATTTCCCAGGTATCGCTAGCGATGGCTACAAAACTTTagaa > 2:164140/1‑140 (MQ=255) cGGAGGTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTATCAGGTATCGCTAGCGATGGCTACAAAACTTTagaa > 2:282131/1‑140 (MQ=255) cGGAGGTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGG‑TTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTAGCGATGGCTACAAAACTTTagaag > 2:318282/1‑140 (MQ=255) gAGGTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAAACGTATTCGTACATTTCTCAGGTATCACTAGCGAAGGCTACAAAAATTTaaaaga > 1:137448/1‑140 (MQ=25) ggTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGAAGTATTCGTACATTTCTCAGGTATCGATAGCAATGGCTACAAAACTTTAGaagaag > 1:166943/1‑140 (MQ=255) tACAGTAAAATGGTTTAACGCAGAAAAATGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTAGCGATGGCTACAAAACTTTAGAAGAAGGTCAAAAAGTTACTTTCGaaa < 2:130249/140‑1 (MQ=255) tACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGTTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTAGCGATGGCTACAAAACTTTAGAAGAAGGTCAAAAAGTTACTTTCGaaa > 2:179525/1‑140 (MQ=255) aGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTAGCGATGGCTACAAAACTTTAGAGGAAGGTCAAAAATTTGTTTTCGAAAtaa > 2:101250/1‑138 (MQ=25) gTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGGCGTATTCGTACATTTCTCAGGTATCGCTAGCGATGGCTACAAAACTTTAGAAGAAGGTCAAAAAGTTACTTTCGAAATCAc > 1:215006/1‑140 (MQ=255) ggTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTAGCGATGGCTACAAAACTTTAGAAGAAGGTCAAAAAGTTACTTTCGAAATCACTGAAGGt < 1:307048/140‑1 (MQ=255) ttAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTAGCGATGGCTACAAAACTTTAGAAGAAGGTCAAAAAGTTACTTTCGAAATCAATGAAGGTCaa > 1:30205/1‑140 (MQ=255) tAACTCATAAAAAGGTTTTGTTTTCATCGAACAAGAAAATTGCTTATACTTATTCTTACATTTCTCAGGTATCGCTAGCGATGGCTACAAAACTTTAGAAGAAGGTCAAAAAGTTACTTTCGAAATCACTGAAGGTCAAc < 1:276291/140‑1 (MQ=255) aaCGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTAGCGATGGCTACAAAACTTTAGAAGAAGGTCAAAAAGTTACTTTCGAAATCACTGAAGGTCAACg > 2:275777/1‑140 (MQ=255) aaaGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTAGCGATGGCTACAAAACTTTAGAAGAAGGTCCAAAAGTTACTTTCGAAATCACTGAAGGTCACCGTGGAGACCa > 2:106834/1‑140 (MQ=255) ttttGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTAGCGATGGCTACAAAACTTTAGAAGAAGGTCAAAAAGTTACTTTCGAAATCACTGAAGGTCAACGTGGAGAACAAgacg > 2:39307/1‑137 (MQ=255) | ATTGTAGTGTATTTGTTTAGAATATCCTCTTTTTTAGTTATGAATTTGTTACAAATATTAAGTGCAAAAGCACACGGAGGTTTTCTATATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATTCGTACATTTCTCAGGTATCGCTAGCGATGGCTACAAAACTTTAGAAGAAGGTCAAAAAGTTACTTTCGAAATCACTGAAGGTCAACGTGGAGACCAAGCAG > CP000730/2864541‑2864809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |